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Digital Gene Expression Analysis of Huanglongbing Affected Mandarins(Citrus reticulata Blanco)In Response to Thermotherapy

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Horticultural Plant Journal 2021年第1期7卷 1-12页

作者： Ruixuan Pan yanling chen Xiaoling Deng Meirong Xu Laboratory of Citrus Huanglongbing Research Guangdong Key Laboratory of Microbial Signals and Disease ControlSouth China Agricultural UniversityGuangzhou 510642China

Huanglongbing(HLB),which is mainly associated with“Candidatus Liberibacter asiaticus”(CLas),is currently threatening citrus production around the *** destructive century-old disease results in callose deposition in phloem plasmodesmata and starch overaccumulation in leaf *** treatment with high humidity has proven to be effective in eliminating the CLas titers in potted citrus *** study explored the curative mechanisms and impact of heat treatment(from 32 to 48°C with 75%±5%relative humidity,14h in light and 10h in dark for four days)on mandarin trees(Citrus reticulata Blanco)that were severely infected by CLas using Solexa/Illumina’s digital gene expression(DGE)profiling.A total of 838 differentially expressed genes(DEGs)were identified,of which 510 were *** interaction analysis of the DEGs indicated that the thermotherapy of HLB trees downregulated the circadian clock related genes in chloroplasts and upregulated the RNA-editing process in the mitochondria,characterized by 141 genes encoding pentatricopeptide repeatcontaining proteins(PPR)being all ***,the expression of phenylpropanoid and flavonoid biosynthesis genes,and auxininduced genes were *** defense response pathway of the thermotherapy treated diseased trees was apparently disrupted,as indicated by the downregulation of LRR receptor-like serine/threonine-protein kinase FLS2 *** study improves our understanding of the curative mechanisms of heat treatment on HLB trees and CLas biological ***,this provides new insights on the involvement of RNA modification by PPR genes and changes in the circadian clock after the heat stress treatment of CLas-infected citrus.

关键词： Citrus huanglongbing heat transcriptom differentially expressed genes

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(N,F)-codoped TiO_2 Nanocrystals as Visible Light-activated Photocatalyst

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Journal of Materials Science & Technology 2009年第1期25卷 73-76页

作者： yanling Meng Jiansong chen Ying Wang Hanming Ding Yongkui Shan Department of Chemistry East China Normal University Shanghai 200062 China

（N, F）-codoped anatase TiO2 nanocrystals with active visible light response were prepared by using a simple sol-gel approach. X-ray photoelectron spectroscopy measurements suggested that the substitutional N and F species replaced the lattice oxygen atoms in TiO2 nanocrystals. Such nanocrystals showed strong absorption from 400 to 550 nm, which was mainly induced by nitrogen doping. The phase transformation from anatase to rutile was hindered by fluorine doping at high calcination temperatures, which was verified by XRD patterns. The N2 adsorption-desorption isotherms revealed the absence of mesopores in these nanocrystals. The （N, F）- codoped TiO2 nanocrystals showed satisfying photocatalytic activity on the photo-degradation of methylene blue under visible light.

关键词： TiO2 nanocrystal (N, F)-codoping Sol-gel method Visible light photocatalyst

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Experimental Study on Impingement Processes of Fuel Sprays on Biomimetic Structured Surfaces

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Journal of Bionic Engineering 2024年第1期21卷 362-373页

作者： yanling chen Liang Guo Wanchen Sun Yuying Yan Rong Xuan Junfeng Zhang State Key Laboratory of Automotive Simulation and Control Jilin UniversityChangchun 130025China Faculty of Engineering University of NottinghamNottingham NG72RDUK

To improve the controllability of the wall-wetting process after the fuel spray-wall impingement in internal combustion engines,the methods of laser etching,chemical etching and surface free energy modification are used to prepare biomimetic structured surfaces with different *** impingement processes of diesel and n-butanol sprays on the walls under different conditions are experimentally *** the surface oleophilicity increases,the spreading radius of wall-impinging sprays *** about 5 s after the fuel injections,the fuel spray droplets hit the walls for the first time,and the secondary breakup and rebound *** mixture concentrations of different fuels hitting the various walls reach the peak *** a higher surface temperature,the peak value of the mixture concentration is mainly related to the heat flux to the fuel droplets in different boiling regimes from the metal *** concentration of the air–fuel mixture in the near wall region increases with increasing surface oleophilicity,increasing wall temperature and decreasing ambient *** with diesel,n-butanol presents a higher air–fuel mixture concentration in the near wall region.

关键词： Spray-wall impingement Morphological development Air-fuel mixture concentration Surface wettability Wall temperature:Ambient pressure

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A new sampler for simulating aerosol deposition in the respiratory tract

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Nuclear Science and Techniques 2008年第3期19卷 169-173页

作者： LI Dehong ZHUO Weihai YI yanling chen Bo LIU Haikuan Institute of Radiation Medicine Fudan University Shanghai 200032 China

For estimation of the deposition fractions of radon progeny in different regions of the respiratory tract, a new sampler consisting of three different configurations of sampling heads was developed. The deposition fractions of aerosols on the wire screens inside the sampling heads were calculated with the fan model of filtration theory. The deposition fractions of aerosols in different regions of the respiratory tract were calculated with the lung dose evaluation program (LUDEP)developed by National Radiological Protection Board (NRPB) as references. In general indoor and mine environments, the deviation between the deposition fractions of attached aerosol on the wire screens designed in this study and its reference values in the respiratory tract is less than 5%. It is possible to accurately estimate the deposition fractions of radon progeny in different regions of the respiratory tract through mimic measurements of radon progeny collected with the new sampler.

关键词：放射性污染环境放射学气溶胶呼吸过程

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Root growth in response to nitrogen supply in Chinese maize hybrids released between 1973 and 2009

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Science China(Life Sciences) 2011年第7期54卷 642-650页

作者： WU QiuPing chen FanJun chen yanling YUAN LiXing ZHANG FuSuo MI GuoHua Key Laboratory of Plant-Soil Interaction MOE College of Resources and Environmental Science China Agricultural University Beijing 100193 China

Root growth has a fundamental role in nitrogen （N） use efficiency. Nevertheless, little is known about how modem breeding progress has affected root growth and its responses to N supply. The root and shoot growth of a core set of 11 representative Chinese maize （Zea mays L.） hybrids released between 1973 and 2009 were investigated under high N （4 mmol L^-1, HN） and low N （0.04 mmol L^-1, LN） levels in a solution culture system. Compared with LN, HN treatment decreased root dry weight （RDW）, the root： shoot ratio （R/S）, and the relative growth rate for root dry weight （RGRroot）, but increased the total root length （TRL） and the total lateral root length （LRL）. The total axial root length （ARL） per plant was reduced under HN, mostly in hybrids released before the 1990s. The number of seminal roots （SRN） was largely unaffected by different N levels. More recently released hybrids showed higher relative growth rates in the shoot under both HN and LN. However, the roots only showed increased RGR under HN treatment. Correspondingly, there was a positive linear relationship with the year of hybrid release for TRL, LRL and ARL under HN treatment. Together, these results suggest that while shoot growth of maize has improved, its root growth has only improved under high N conditions over the last 36 years of selective breeding in China. Improving root growth under LN conditions may be necessary to increase the N use efficiency of maize.

关键词： Root R/S ratio nitrogen relative growth rate maize breeding

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Automatic selection of permanent scatterers-based GCPs for refinement and reflattening in InSAR DEM generation

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International Journal of Digital Earth 2022年第1期15卷 954-974页

作者： Yongjiu Feng Yilun Zhou yanling chen Pengshuo Li Mengrong Xi Xiaohua Tong College of Surveying & Geo-Informatics Tongji UniversityShanghaiPeople’s Republic of China The Shanghai Key Laboratory of Space Mapping and Remote Sensing for Planetary Exploration Tongji UniversityShanghaiPeople’s Republic of China Shanghai Astronomical Observatory Chinese Academy of SciencesShanghaiPeople’s Republic of China

In DEM generation using interferometric synthetic aperture radar(InSAR),the ground control points(GCPs)for refinement and reflattening are usually selected by manual selection,field surveying,GPS points and existing basemaps,which may not be completely suitable for consequent *** proposed a new method(auto-PS-GCP)of GCP selection based on permanent scatterers,which automatically defines the thresholds for the coherence,amplitude,and amplitude dispersion index to select permanent scatterer as the *** GCP thinning(auto-PS-GCP-Thin)was further conducted considering the point density,distances among points and terrain *** used a three-stage assessment that includes:(1)phase stability and intensity of the GCPs,(2)RMSEs of the elevations between GCPs and homonymous points in the reference DEM,and(3)generated DEM *** areas respectively in the plain,hilly and mountainous regions were selected to verify the proposed *** assessment using both SRTM DEM andICESat-2 points shows that the DEM accuracy of auto-PS-GCP-Thin was improved by 20%∼30%for different areas compared to the manual,where the best DEM accuracy of 4.71 m was found in the plain *** is concluded that the proposed methods are effective and reliable in various areas with different terrain conditions.

关键词： InSAR permanent scatterers automatic threshold definition ground control points DEM generation

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Precise absolute positioning for a single-frequency user

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Science China(Physics,Mechanics & Astronomy) 2013年第8期56卷 1591-1597页

作者： LI HaoJun TANG GuiFeng HE Feng WU Bin HU XiaoGong chen yanling Shanghai Astronomical Observatory Chinese Academy of Sciences Guangxi Key Laboratory of Spatial Information and Geomatics Guilin University of Technology Shanghai Key Laboratory of Space Navigation and Position Techniques Beijing Satellite Navigation Center

A new strategy to realize precise absolute positioning for a single-frequency user is presented. In the presented strategy, the receiver clock and ambiguities are removed using the satelliteand epoch-differenced (SDED) algorithm. As a further development of the SDED algorithm, a regional augmentation network is used to generate the SDED atmospheric delays at the user. The weakened mathematic property due to the epoch-differenced operation is improved by adding the generated atmospheric delays and applying the robust estimation. To test the new approach, the 24-h data at 5 Continuous Operation Reference Station (CORS) stations in Shanghai is processed. The results show a more than 96% success rate, defined as the case where three directions achieve the desired positioning accuracy of 10 cm, when the observation is longer than 20 min. The 20-min static results show that the new method can reach an accuracy of 3.42, 4.76 and 9.26 cm in the North, East and Up directions, respectively. An experiment was carried out to assess the kinematic positioning. The results show that the kinematic positioning accuracy is 4.11, 5.31 and 4.05 cm in the north-south, east-west and height directions,respectively.

关键词： absolute positioning single-frequency user correction information

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Evaporation Characteristics and Morphological Evolutions of Fuel Droplets After Hitting Different Wettability Surfaces

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Journal of Bionic Engineering 2023年第2期20卷 734-747页

作者： yanling chen Liang Guo Ningning Cai Wanchen Sun Yuying Yan Degang Li Han Wang Rong Xuan State Key Laboratory of Automotive Simulation and Control Jilin UniversityChangchun 130025China School of Business and Management Jilin UniversityChangchun130012China Faculty of Engineering University of NottinghamNottinghamNG72RDUK

To solve the wall-wetting problem in internal combustion engines,the physical and chemical etching methods are used to prepare different wettability surfaces with various *** evaporation characteristics and morphological evolution processes of diesel and n-butanol droplets after hitting the various surfaces are *** results show that the surface microstructures increase the surface roughness(Ra),enhancing the oleophilic property of the oleophilic *** with n-butanol droplets,the same surface shows stronger oleophobicity to diesel *** a droplet hits an oleophilic property surface with a lower temperature,the stronger the oleophilicity,the shorter the evaporation *** oleophilic surfaces,larger Ra leads to a higher Leidenfrost temperature(TLeid).The low TLeid caused by enhanced oleophobicity,dense microstructures and increased convex dome height facilitates droplet rebound and promotes the evaporation of the wall-impinging droplets into the *** evaporation rate of the droplets is not only related to the characteristics of the solid surfaces and the fuel droplets but also affected by the heat transfer rate to the droplets in different boiling *** spreading diameter of a droplet on an oleophobic surface varies significantly less with time than that on an oleophilic surface under the same surface temperature.

关键词： Fuel droplets Wettability Evaporation time Leidenfrost temperature Morphological evolutions

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Coordinated Transcriptional Regulation by the UVB Photoreceptor and Multiple Transcription Factors for Plant UV-B Responses

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Molecular Plant 2020年第5期13卷 777-792页

作者： Chongzhen Qian Zhiren chen Qing Liu Weiwei Mao yanling chen Wei Tian Yan Liu Jiupan Han Xinhao Ouyang Xi Huang State Key Laboratory of Cellular Stress Biology School of Life SciencesXiamen UniversityXiamen 361102China

Non-damaging ultraviolet B(UV-B)light promotes photomorphogenic development and stress acclimation through UV-B-specific signal transduction in ***-B irradiation induces monomerization and nuclear translocation of the UV-B photoreceptor UV RESISTANCE LOCUS 8(UVR8).However,it is not clear how the nuclear localization of UVR8 leads to changes in global gene ***,we reveal that nuclear UVR8 governs UV-B-responsive transcriptional networks in concert with several previously known transcription factors,including ELONGATED HYPOCOTYL 5(HY5)and PHYTOCHROME INTERACTING FACTOR 4(PIF4).Based on the transcriptomic analysis,we identify MYB13 as a novel positive regulator in UV-B-induced cotyledon expansion and stress ***13 is UV-B inducible and is predominantly expressed in the *** results demonstrate that MYB13 protein functions as a transcription factor to regulate the expression of genes involved in auxin response and flavonoid biosynthesis through direct binding with their *** addition,photoactivated UVR8 interacts with MYB13 in a UV-B-dependent manner and differentially modulates the affinity of MYB13 with its *** together,our results elucidate the cooperative function of the UV-B photoreceptor UVR8 with various transcription factors in the nucleus to orchestrate the expression of specific sets of downstream genes and,ultimately,mediate plant responses to UV-B light.

关键词： UV-B UVR8 MYB13 transcription factor transcriptional regulation

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Jiaweisinisan facilitates neurogenesis in the hippocampus after stress damage

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Neural Regeneration Research 2013年第12期8卷 1091-1102页

作者： Lili Wu Chuanlian Ran Shukao Liu Lizhen Liao yanling chen Hualei Guo Weikang Wu Can Yan Department of Basic Theory of Chinese Medicine Guangzhou University of Chinese Medicine Department of Traditional Chinese Medicine Luzhou Medicine College Institute of Pharmaceutical Research Yangtze River Pharmaceutical Group Company Limited Department of Pathophysiology Zhongshan School of Medicine Sun Yat-sen University

The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 IJM corticosterone. Cells were treated with 10% （v/v） Jiaweisinisan drug-containing serum and the corticosterone antagonist RU38486. Results of the 3-（4,5-dimethylthiazol-2-yl）-3,5-di-phenytetrazoliumromide assay showed that both Jiaweisinisan drug-containing serum and RU38486 promoted the proliferation of neural precursor cells after corticosterone exposure. Immunofluorescence detection showed that after Jiaweisinisan drug-containing serum and RU38486 treatment, the 5-bromo-2-deoxyuridine/terminal deoxynucleotidyl transferase dUTP nick end labeling ratio in hippocampal neural precursor cells significantly increased, and the apoptotic rates of glial cells reduced, and neuron-like cell differentiation from neural precursor cells significantly increased. Our experimental findings indicate that Jiaweisinisan promotes hippocampal neurogenesis after stress damage.

关键词： neural regeneration traditional Chinese medicine Jiaweisinisan corticosterone hippocampalneural precursor cells proliferation differentiation apoptosis grants-supported paper neuroregeneration

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