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Different Transcription Profiles of SOCS-3,ob and IGF-I Genes and their Possible Correlations in Obese and Lean Pigs

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 305-310页

作者： Jiangwei WU Bo WANG Haowei ZHANG Taiyong YU Gongshe YANG* Laboratory of Animal Fat Deposition and Muscle Development.Northwest A&F University,yangling 712.00,China LaboratoryofAnimalFatDepositionandMuscleDevelopment NorthwestA&FUniversityYangling712100China

Pig breeds have significant differences in fat deposition and muscle development ***,the molecular mechanism behind these differences is still *** this study,the expressionpatterns of three candidate genes,suppressor of cytokine signaling 3(SOCS-3),obesity(oh)and insulin-likegrowth factor Ⅰ(IGF-1),which are involved in adipose metabolism or muscle development,were *** RNA was extracted from dorsal subcutaneous adipose tissue and longissimus of 8-month-old Bameiand Largewhite *** reverse transcription-polymerase chain reaction was used to deter-mine the expression levels of the SOCS-3 and ob genes in adipose tissue,and SOCS-3 and IGF-I genes inmuscle *** results showed that in adipose tissue the expression level of SOCS-3 was significantlyhigher in Bamei(obese)pigs than that in Largewhite(lean)pigs(P<0.01).However,in muscle tissue it wassignificantly lower in Bamei than that in Largewhite pigs(P<0.01).Furthermore,the expression of SOCS-3was positively correlated to that of ob in adipose tissue and that of IGF-I in muscle *** findingssuggest that the difference in SOCS-3 gene expression levels in adipose and muscle tissues,the relationshipbetween SOCS-3 and ob in adipose tissue,and that between SOCS-3 and IGF-I in muscle tissue,mightcontribute to the different fat deposition and muscle development ability between obese and lean pigs.

关键词： SOCS-3 ob IGF-I fat deposition muscle development obese pigs lean pigs

A hyper-chaos with only one nonlinear term and its adaptive synchronization and control

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A hyper-chaos with only one nonlinear term and its adaptive ...

运用灰色灾变理论对黄淮海流域旱情的长期预测

2010 Chinese Control and Decision Conference

作者： CHEN Di-yi Electric Department NorthWest A&F University Shanxi yangling 712.00,China MA Xiao-yi Electric Department NorthWest A&F University Shanxi yangling 712.00,China

＜正＞Chaos is widespread in natural session, which is one of typical nonlinear *** paper gave a four-dimensional nonlinear dynamic system with only one nonlinear *** it also analyzed its complex dynamic characteristics,including the space trajectory,the Lyapunov exponent,the Poincare map, the Time-domain waveform diagram and the Bifurcation *** characteristics indicate that the four-dimensional dynamical systems contain hyper-chaotic *** designed adaptive controller,and achieved the system’s synchronization and *** results show that using adaptive synchronization and control methods can be an ideal way to eliminate the chaos,so that the hyper-chaotic system can reach a stable state,and also show the validities and feasibilities of adaptive synchronization and control methods to control hyper-chaotic dynamical systems.

关键词： Single nonlinear term Four-dimensional hyper-chaos system adaptive synchronization control chaos control

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干旱地区农业研究 1989年第4期7卷 64-70页

作者：贾志宽西北农业大学

本文用灰色灾变理论对黄淮海流域地区的旱情进行了长期预测。12个预测模型中,经检验11个都达到了一级水平(GOOD)。预测结果表明:2000年前,1997年将是黄淮海流域地区最大的干旱年,不仅旱情重而且持续时间长;1990年春旱范围大,旱情将持续2... 详细信息

本文用灰色灾变理论对黄淮海流域地区的旱情进行了长期预测。12个预测模型中,经检验11个都达到了一级水平(GOOD)。预测结果表明:2000年前,1997年将是黄淮海流域地区最大的干旱年,不仅旱情重而且持续时间长;1990年春旱范围大,旱情将持续2—3个月;1993年春夏连旱;1998年为夏秋连旱。

关键词：灰色灾变理论黄淮海流域旱情

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无机营养与作物抗旱性的关系

干旱地区农业研究 1989年第4期7卷 77-84页

作者：徐萌中国科学院水利部西北水土保持研究所

本文总结概述了氮、磷、钾三种大量营养元素与作物抗旱性关系的研究结果,认为作物对干旱的适应方式是多途径的,然而无机营养对作物抗旱性各种机制的影响并不都是完全一致的,有的作用利于作物抗旱性提高,有的作用则不利于作物的抗旱性。... 详细信息

本文总结概述了氮、磷、钾三种大量营养元素与作物抗旱性关系的研究结果,认为作物对干旱的适应方式是多途径的,然而无机营养对作物抗旱性各种机制的影响并不都是完全一致的,有的作用利于作物抗旱性提高,有的作用则不利于作物的抗旱性。在某种程度上无机营养也影响作物的生理抗旱机制。在干旱条件下,一般高营养水平作物叶片气孔导度的下降幅度比低营养水平作物叶片气孔导度的下降幅度大。无机营养对作物渗透节调影响的研究结果不一致,然而无机营养水平提高能够促进游离脯氨酸积累,这一结果是共同的。在耐脱水性方面,高营养水平作物原生质的耐脱水性和原生质膜的稳定性都增强。

关键词：无机营养作物抗旱性

cDNA Cloning of Goat DNA Methyltransferase 1,Screening of shRNA Vectors and Influences to Development of Nuclear Transfer Embryos

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Agricultural Sciences in China 2010年第7期9卷 1035-1040页

作者： LAN Jie,SONG Yong-li,HUA Song,LIU Yong-gang,LIU Jun,ZHANG Hai-lin and ZHANG Yong Key Laboratory of Animal Reproductive Physiology & Embryo Technology,Institution of Biotechnology,College of Veterinary Medicine,Northwest A&F University,yangling 712.00,P.R.China [a] Key Laboratory of Animal Reproductive Physiology & Embryo Technology Institution of Biotechnology College of Veterinary Medicine Northwest A&F University Yangling 712100 P.R. China

This study was designed to clone cDNA of goat DNA methyltransferase 1（DNMT1） gene,to screen an effective shRNAproducing vector targeting goat DNA methyltransferase 1 and to improve the developmental competence of goat nuclear transfer embryos by decreasing the DNMT1 expression in donor *** this study,PCR primers were designed against regions of high homology between bovine and sheep sequences and then used to amplify the larger portions of the coding ***,3 RNAi oligonucleotides were designed based on the cloned sequences and inserted into pRNAT-U6.1/Neo vector,acquiring 3 new vectors,respectively termed pRNAD1,pRNAD2 and *** the positive cells were sorted by flow cytometry after transfection and detected by real-time PCR analysis and sodium bisulfite genomic ***,the developmental rates of nuclear transfer（NT） embryos generated using donor cells with and without the effective shRNA vector respectively,as well as in vitro fertilization（IVF） embryos were observed and *** results showed that the coding regions of goat DNA methyltransferase 1 gene was successfully cloned（GenBank ***617538）.Furthermore,an effective interfering shRNA（pRNAD2） was obtained,with its interference effect being 47.88%.Finally,NT embryos with shRNA vector harbored better developmental competence during morula and blastocyst stage compared to controls（P 〈 0.05）,reaching the similar rates to IVF embryos（P 〉 0.05）.In conclusion,goat DNA methyltransferase 1 gene cDNA was cloned and sequenced,an effective shRNA vector responsible for inhibiting DNA methyltransferase 1 expression was developed and the developmental competence of goat nuclear transfer morulae and blastcysts was significantly improved,which provided a feasible pathway for improving goat nuclear transfer embryo development competence by decreasing the methylation level in donor cells through RNAi-mediated manner.

关键词： cDNA DNA methyltransferase 1 goat methylation shRNA

A Novel Chemical Hybridized Agent and Its Male Sterility-inducing Mechanism in Rapeseed

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A Novel Chemical Hybridized Agent and Its Male Sterility-ind...

Immune regulation of hydrogen sulfide in children with acute lymphoblastic leukemia

第十二届全国植物基因组学大会

作者： Yufeng Cheng,Huixian Zhao~* Northwest A&F University,yangling,Shaanxi 712.00,China

Significant heterosis for seed yield and other agronomic traits is well documented in oilseed *** hybridizing agent(CHA) is becoming a promise way for this heterosis.A great deal of achievements has been made in *** group focused on development of new CHAs and its male sterility-inducing mechanism with the finical support of the earmarked fund for Modern Agro-industry Technology Research System. After several years’ work,a new CHA1 were successfully identified from herbicides developed in *** male sterility inducing-ability and its molecular mechanism of CHA1 are briefly introduced in this report.

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Chinese Medical Journal 2014年第21期127卷 3695-3699页

作者： Du Shuxu Jia Yongrui Tang Hong Sun yangling Wu Wanshui Sun Liming Du Junbao Geng Bin Tang Chaoshu Jin Hongfang Department of Pediatrics Shijitan Hospital Capital Medical University Beijing 100038 China Health Science Center Peking University Beijing 100191 China Department of Pediatrics Peking University First Hospital Beijing 100034 China Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100191 China

Background Acute lymphoblastic leukemia （ALL） and chemotherapy can cause immune imbalance,and gaseous molecule hydrogen sulfide （H2S） can participate in the process of immune *** study aimed to investigate the immune regulation of H2S in pediatric *** Children （n=78） with ALL admitted during 2010-2013 were included in this *** blood samples were collected in period of before chemotherapy,bone marrow remission and two days after chemotherapy,*** contents of H2S and cytokines,including interleukin-1β （IL-13）,interleukin-2 （IL-2）,interferon-γ （IFN-γ）,tumor necrosis factor （TNF-α）,interleukin-4 （IL-4）,interleukin-6 （IL-6）,interleukin-10 （IL-10） and macrophage inflammatory protein-1α （MIP-1a）,were detected using ELISA *** regression was used to analyze the correlation between H2S and ***,human Jurkat cells were cultured in vitro,and nucleoprotein of Jurkat cells and peripheral blood mononuclear cells （PBMCs） were collected,contents of cystathionine γ-lyase （CSE） and certain cytokines were measured by Western *** Serum concentrations of H2S,IL-13,IL-6,IL-10 and MIP-1α in children with ALL were increased significantly （P ＜0.01）,while concentrations of IL-2,TNF-α,IFN-γ and IL-4 decreased obviously （P ＜0.01）.In patients after chemotherapy,concentrations of H2S and IL-10 were decreased significantly （P ＜0.05）,but IL-4 and IFN-γ concentrations increased markedly （P ＜0.05）.At remission stage,H2S,IL-13,IL-4,IL-6,IL-10 and MIP-1α concentrations were further decreased markedly （P ＜0.05）,but concentrations of IL-2,TNF-α and IFN-γ increased again （P ＜0.05）.Protein contents of CSE,IL-10,IL-4 and IL-2 of PBMCs also increased markedly in children with ***,changes of CSE protein contents of PBMCs were consistent with serum H2S contents,and there were significant correlation between H2S and certain cytokines based on stepwise regression ***,compared with those of PBMCs group,in vit

关键词： child acute lymphoblastic leukemia hydrogen sulfide cytokine immune

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渭北旱塬麦田水分循环及水分亏缺研究

水土保持学报 1994年第3期8卷 87-91页

作者：毛瑞洪陕西省农业科学院

渭北旱塬麦田土壤水分支出的主要途径是蒸散作用，收入则靠降水。经在本区的永寿县民丰村定点定位采用仪器观测，所取结果用平衡公式计算，麦田水分的收支基本入不敷出（降水量－麦田蒸散量）。因此，干旱缺水是该区小麦生产的主要限制... 详细信息

渭北旱塬麦田土壤水分支出的主要途径是蒸散作用，收入则靠降水。经在本区的永寿县民丰村定点定位采用仪器观测，所取结果用平衡公式计算，麦田水分的收支基本入不敷出（降水量－麦田蒸散量）。因此，干旱缺水是该区小麦生产的主要限制因素，因而抗旱保墒也就成为当地小麦增产的主要措施之一。

关键词：麦田水分蒸发量土壤水分平衡

THE CHAIN DEPENDENCE OF DAILY PRECIPITATION OCCURRENCE IN SHAANXI PROVINCE OF CHINA

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Acta meteorologica Sinica 1989年第2期3卷 250-259页

作者：钱允祺 The Northwestern Agricultural University YanglingShaanxi Province

Daily precipitation records for summer months at fourteen stations in Shaanxi Province,China,have been analysed to determine the dependence of daily precipitation occurrence by use of MAICE and MBICE *** results show that dependence is statistically significant at all fourteen stations located in different climatic *** of Markov chain models gives a satisfactory description of the distributions of wet and dry sequences,including long-length spells.

关键词： AIC THE CHAIN DEPENDENCE OF DAILY PRECIPITATION OCCURRENCE IN SHAANXI PROVINCE OF CHINA OO BIC OU 口心