检索结果-南通市图书馆

经皮肾动脉去交感神经术在慢性收缩性心力衰竭患者中的应用研究

在线全文

学校读者我要写书评

暂无评论

临床心血管病杂志 2016年第10期32卷 1005-1008页

作者：郜俊清杨伟刘宗军上海市普陀区中心医院心内科上海200333

目的:研究经皮肾动脉去交感神经术(RDN)对慢性收缩性心力衰竭患者心功能改善的作用。方法:将60例慢性收缩性心力衰竭患者分为2组,其中RDN组接受双侧肾动脉去神经治疗,对照组仅接受药物治疗。对所有的患者进行6个月的随访,观察N末端脑钠... 详细信息

目的:研究经皮肾动脉去交感神经术(RDN)对慢性收缩性心力衰竭患者心功能改善的作用。方法:将60例慢性收缩性心力衰竭患者分为2组,其中RDN组接受双侧肾动脉去神经治疗,对照组仅接受药物治疗。对所有的患者进行6个月的随访,观察N末端脑钠肽前体(NT-pro BNP)、6min步行距离、心功能、血压及生化指标的变化。结果:6个月随访时,与对照组比较,RDN组左室射血分数(LVEF)显著提高[(39.1±7.3)%:(35.6±3.3)%,P<0.05],NT-proBNP显著下降[(440.1±226.5)pg/ml:(790.8±287.0)pg/ml,P<0.05],6min钟步行距离显著提高[(301.2±139.5)m:(227.2±65.0)m,P<0.05],血压显著下降[(123.3±68.5)mmHg:(139.8±80.2)mmHg,P<0.05]。结论:RDN可能是一种有效的治疗慢性心力衰竭的新技术,可有效提高患者LVEF,改善心功能,提高运动耐量。

关键词：肾去神经化交感神经系统心力衰竭血压

Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第11期20卷 3302-3316页

作者： Yali Chen Yiyong wei Jin liu Tao Zhu Cheng Zhou Donghang Zhang Department of Anesthesiology West China HospitalSichuan UniversityChengduSichuan ProvinceChina Laboratory of Anesthesia and Critical Care Medicine National-Local Joint Engineering Research Center of Translational Medicine of AnesthesiologyWest China HospitalSichuan UniversityChengduSichuan ProvinceChina Department of Anesthesiology Longgang District Maternity&Child Healthcare Hospital of Shenzhen City(Longgang Maternity and Child Institute of Shantou University Medical College)ShenzhenGuangdong ProvinceChina Department of Anesthesiology Affiliated Hospital of Zunyi Medical UniversityZunyiGuizhou ProvinceChina

Glial cells play crucial roles in regulating physiological and pathological functions,including sensation,the response to infection and acute injury,and chronic neurodegenerative *** cells include astrocytes,microglia,and oligodendrocytes in the central nervous system,and satellite glial cells and Schwann cells in the peripheral nervous *** the greater understanding of glial cell types and functional heterogeneity achieved through single-cell and single-nucleus RNA sequencing in animal models,few studies have investigated the transcriptomic profiles of glial cells in the human spinal ***,we used high-throughput single-nucleus RNA sequencing and spatial transcriptomics to map the cellular and molecular heterogeneity of astrocytes,microglia,and oligodendrocytes in the human spinal *** explore the conservation and divergence across species,we compared these findings with those from *** the human spinal cord,astrocytes,microglia,and oligodendrocytes were each divided into six distinct transcriptomic *** the mouse spinal cord,astrocytes,microglia,and oligodendrocytes were divided into five,four,and five distinct transcriptomic subclusters,*** comparative results revealed substantial heterogeneity in all glial cell types between humans and ***,we detected sex differences in gene expression in human spinal cord glial ***,in all astrocyte subtypes,the levels of NEAT1 and CHI3L1 were higher in males than in females,whereas the levels of CST3 were lower in males than in *** all microglial subtypes,all differentially expressed genes were located on the sex *** addition to sex-specific gene differences,the levels of MT-ND4,MT2A,MT-ATP6,MT-CO3,MT-ND2,MT-ND3,and MT-CO_(2) in all spinal cord oligodendrocyte subtypes were higher in females than in ***,the present dataset extensively characterizes glial cell heterogeneity and offers a valuable resource for explo

关键词： astrocyte glial cell human microglia oligodendrocyte sex differentiation single-nucleus RNA sequencing spatial transcriptomics species variation spinal cord

在线全文

学校读者我要写书评

暂无评论

甜菜树贝壳杉烯合酶基因的克隆与原核表达

食品与生物技术学报 2017年第5期36卷 479-485页

作者：刘洪伟王帅王强王伟杨艳芳刘锡葵邱德有林木遗传育种国家重点实验室中国林业科学研究院林业研究所北京100091 四川农业大学农学院成都611130 中国医学科学院/中国协和医科大学药物研究所北京100050 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室云南昆明650204

本文作者研究了赤霉素合成途径中的关键酶-贝壳杉烯合酶,对其功能的研究可以为以后优化品种提供基因层面的基础。首先通过RACE-PCR技术,从甜菜树叶片中克隆得到贝壳杉烯合酶基因(Ent-kaurene synthase gene,Yl KS)的全长c DNA,共2 512 b... 详细信息

本文作者研究了赤霉素合成途径中的关键酶-贝壳杉烯合酶,对其功能的研究可以为以后优化品种提供基因层面的基础。首先通过RACE-PCR技术,从甜菜树叶片中克隆得到贝壳杉烯合酶基因(Ent-kaurene synthase gene,Yl KS)的全长c DNA,共2 512 bp(Gen Bank登录号为KP872698),其ORF长度为2 232 bp,共编码743个氨基酸,预测蛋白质相对分子质量大小为84 987,蛋白质等电点为5.264,表明该蛋白质呈酸性。将该基因构建到表达载体pET32a中,得到重组质粒pET-32a-Yl KS。通过将p GG/An2、p IRS和重组质粒pET-32a-Yl KS 3个质粒共转入菌株BL21(DE3)中,得到重组菌株,并进行发酵。通过SDS-PAGE分析发现,重组蛋白成功得到了表达。最后通过对发酵产物进行萃取,并使用GC-MS进行检测,确定了该基因所编码的酶确实是贝壳杉烯合酶。

关键词：甜菜树贝壳杉烯合酶原核表达气质联用

Clinical experience of defect reconstruction after total glossectomy and total laryngectomy with pedicled pectoralis major myocutaneons flap

在线全文

学校读者我要写书评

暂无评论

中国口腔颌面外科杂志 2008年第B5期6卷 214-215页

作者： Lai Ping Zhong Zhi Yuan Zhang Chen Ping Zhang Han Guang Zhu Jian Sun Yong Jie Hu Tong Ji Jun Li wei Min Ye Yue He Wen Jun yang wei liu Qiu Department of Oral and Maxillofacial Surgery Ninth People'sHospital School of Medicine Shanghai Jiao Tong University200011 China

扑草净和扑灭通在土壤中吸附及其与色谱热力学函数的相关性

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

土壤学报 2002年第5期39卷 693-698页

作者：杨炜春刘维屏马云刘惠君浙江大学玉泉校区环境科学研究所杭州310027

本研究比较了除草剂扑草净和扑灭通在 6种土壤中的吸附 ,采用Freundlich方程对其吸附等温线进行描述 ,对Freundlich方程吸附常数Kaf与土壤理化性质的相关性进行了分析 ,由于反相高效液相色谱流动相组成与扑草净和扑灭通容量因子成线性关... 详细信息

本研究比较了除草剂扑草净和扑灭通在 6种土壤中的吸附 ,采用Freundlich方程对其吸附等温线进行描述 ,对Freundlich方程吸附常数Kaf与土壤理化性质的相关性进行了分析 ,由于反相高效液相色谱流动相组成与扑草净和扑灭通容量因子成线性关系 ,从而测定了扑草净和扑灭通的过量热力学函数ΔH ,证明扑草净和扑灭通在土壤中的吸附与ΔH 存在一定的关系 ,从而提出ΔH 和ΔS 可作为研究农药性质的重要参数之一。

关键词：扑草净扑灭通土壤过量热力学函数吸附脱附除草剂高效液相色谱

Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： wei Zhang Yubao Lu Ruoqi Shen Yingjie Wu Chenrui liu Xingxing Fang Liangming Zhang Bin liu Limin Rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Cell Products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

A novel active-passive protection strategy endows carbonyl iron with excellent intelligent anti-corrosion and microwave absorption dual functional characteristics

在线全文

学校读者我要写书评

暂无评论

Nano Research 2025年第1期18卷 487-500页

作者： wei Tian Quanyuan Feng Linbo Zhang Yinfan liu Xian Jian Longjiang Deng Institute of Microelectronics School of Information Science and TechnologySouthwest Jiaotong UniversityChengdu 611756China National Engineering Researching Centre of Electromagnetic Radiation Control Materials Key Laboratory of Multi-Spectral Absorbing Materials and Structures of Ministry of EducationState Key Laboratory of Electronic Thin Films and Integrated DevicesSchool of Electronic Science and EngineeringUniversity of Electronic Science and Technology of ChinaChengdu 611731China School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731China The yangtze Delta Region Institute(Huzhou)&School of Electronic Science and Engineering University of Electronic Science and Technology of ChinaHuzhou 313001China

Carbonyl iron absorbers(CI)face significant challenges in practical applications,such as corrosion,interface bonding failure,detachment,and high maintenance ***,we have developed intelligent self-healing technology based on proactive/passive mechanisms via in situ synthesis of self-healing factors(polydopamine/benzotriazole(PDA/BTA))and physical barrier layers(SiO_(2)/1,1,1,3,3,3-hexamethyl disilazane,SiO_(2)/HMDS)to enhance corrosion resistance,while also being compatible with efficient microwave absorption *** unique multiscale structure gives full play to the utilization of the roles of each functional layer,including the intelligent self-healing features of PDA/BTA,physical shielding and spatial confinement characteristics of SiO_(2)/HMDS,and magnetic-dielectric synergistic mechanism resulted from the good impedance matching characteristics,the conduction loss,the interfacial polarization loss and natural *** asfabricated composites achieved an exceptional minimum reflection loss value of-55.4 dB at 10.7 GHz and the effective absorption band of 7.6 ***,it still exhibits obvious self-healing and corrosion resistance characteristics after 360 h corrosion treatment,ascribed to the self-healing mechanism of PDA/BTA and the blocking intervention effect of SiO_(2)/*** work is considered to pave the way for the synthesis of high-performance magnetic absorbers,especially in enhancing their intelligent self-healing ability in corrosive environments.

关键词： microwave absorption intelligent self-healing technology carbonyl iron multiscale structure magneticdielectric synergistic mechanism

我国农业上市公司财务竞争力综合评价实证研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

科技与管理 2008年第5期10卷 36-39页

作者：杨位留山东经济学院会计学院山东济南250014

财务竞争力是现代财务学科体系中一个新的领域,当前财务管理理论中新的研究方向,以我国沪深两市A股农业类上市公司为研究样本,采用了企业效绩评价体系中的部分财务指标,运用因子分析方法对企业财务竞争力进行综合评价与分析,以期对上市... 详细信息

财务竞争力是现代财务学科体系中一个新的领域,当前财务管理理论中新的研究方向,以我国沪深两市A股农业类上市公司为研究样本,采用了企业效绩评价体系中的部分财务指标,运用因子分析方法对企业财务竞争力进行综合评价与分析,以期对上市公司的财务竞争力综合评价提供参考,为投资者进行投资决策和行政职能部门制定宏观管理策略提供支持。

关键词：财务竞争力因子分析综合评价

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang Xin liu Jian Zhang Ya Gao Shuyue Li Cui Chang Xiang liu Guofeng yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein