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Depletion of phosphatidyl-glycerol head-group induces changes in oxygen evolution and protein secondary structures of photosystem Ⅱ

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chinese Science Bulletin 2002年第10期47卷 824-829页

作者： yang zhenle, WANG Zeneng, JIANG Guizhen, XU Yinong, li liangbi & kuang tingyunphotosynthesis research center, institute of botany, chinese academy of sciences, beijing 100093. china Photosynthesis research center Institute of Botany Chinese Academy of Sciences Beijing China

The techniques of oxygen electrode polarogra-phy and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and structural aspects of photosystem Ⅱ (PS Ⅱ) through enzymatic approach. It was shown that the depletion of PG by treatment of phospholipase C (PLC) on PS Ⅱ particles caused the inhibition of oxygen evolving activity in PS Ⅱ. This effect also gave rise to changes in the protein secondary structures of PS Ⅱ, that is, an increase in a-helical conformation which is compensated by the loss of p-strand structures. It revealed that the head-group of PG molecules plays an important structural role in the maintenance of normal structure of PS Ⅱ proteins, which is required to maintain the appropriate physiological activity of the PS Ⅱ complex such as the oxygen evolving activity. It is suggested that there most probably exist hydrogen-bonding interactions between PG molecules and PS Ⅱ proteins.

关键词： photosystem II polar head-group of phosphatidylglyc-erol phospholipase C oxygen evolution protein secondary struc-ture.

Phosphatidylglycerol effect on oxygen-evolving activity in Ca^(2+)-depleted photosystem Ⅱ

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chinese Science Bulletin 2000年第21期45卷 1964-1968页

作者： yang zhenle, li liangbi, XU Yinong & kuang TingyunLaboratory of Photosynthesis Basic research, institute of botany, chinese academy of sciences, beijing 100093. china Laboratory of Photosynthesis Basic research Institute of Botany Chinese Academy of Sciences Beijing China

The ettect of anionic phosphatidylglycerol (HG) on oxygen evolution in a photosystem Ⅱ (PS Ⅱ) particle depleted of Ca2+ (designated dCaPS Ⅱ) has been investigated. The major finding is the observation of a new role of PG in the PS Ⅱ function. That is, PG restores nearly the lost oxygen evolution in dCaPS Ⅱ particle owing to Ca2+ depletion to the levels in intact PS Ⅱ . Furthermore, there is a stimulation of oxygen-evolving activity in the dCaPSⅡ complexed with PG in the presence of exogenous CaCI2, which PG enhances increasingly oxygen evolution with increasing CaCI2 concentration. It is suggested that PG-induced oxygen evolution recovery of dCa PS Ⅱresumption of normal structure in protein by PG effect, whereas the enhancement of oxygen evolution in complex subject to CaCI2 is ascribed to the optimization of such a structure due to coordination complex formation of Ca2+ ions with PG.

关键词： photosystem Ⅱ Ca2+-depleted photosystem Ⅱ phosphatidylglycerol Ca2+ oxygen-evolving activity.

Increase in electron transfer activity in photosystemⅡof spinach thylakoids caused by conversion of phosphatidyl-glycerol to phosphatidic acid molecules

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chinese Science Bulletin 2003年第15期48卷 1581-1585页

作者： WU Feng, yang zhenle, li liangbi & kuang Tingyun Key Laboratory of Photosynthesis and Environmental Molecular Physiology & Photosynthesis research center, institute of botany, chinese academy of sciences, beijing 100093. china Correspondence should be addressed to yang zhenle (e-mail: yangzhl @***) Key Lab. of Photosyn. and Environmental Molec. Physiology and Photosynthesis research center Institute of Botany Chinese Academy of Sciences Beijing 100093 China

The techniques of oxygen electrode polarography, sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) and thin layer chromatography (TLC) were employed to investigate the effect of phospholipase D treatment on physiological function of spinach thylakoids. It was shown that the phospholipase D treatment on thylakoid resulted in the degradation of phosphatidylglycerol (PG) and occurrence of phosphatidic acid (PA). The changes of PG to PA molecules caused an increase in oxygen evolution in photosystem Ⅱ (PSⅡ), which was accompanied by an uncoupling effect on thylakoid membrane. It was revealed that the head-groups of PG molecules play an important role in the maintenance of the appropriate physiological activity of thylakoid membrane.

关键词：电子转移活性类囊体光合作用菠菜磷脂酰甘油

The structural model of P_(680)

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chinese Science Bulletin 1999年第9期44卷 862-863页

作者： ZHANG Chunxi , ZHANG Xiaodong , li liangbi , ZHANG Qiyuan , ZHANG Xingkang and kuang tingyunphotosynthesis research center and Laboratory of Photosynthesis Basic research , institute of botany , chinese academy of sciences , beijing 100093. china State Key Laboratory for Structural Chemistry of Unstable and Stable Species , institute of Chemistry, chinese academy of sciences , beijing 100080, china Photosynthesis research center and Laboratory of Photosynthesis Hasic research Institute of Botany Chinese Academy of Sciences Beijing China State Key Laboratory for Structural Chemistry of Unstable and Stuble Species. institute of Chemistry Chinese Academy of Sciences Beijing China

INVESTIGATION on photosystem Ⅱ （PS Ⅱ ） of high plants is one of the most important aspects in photosynthesis research. P680 is the primary electron donor in reaction center of PS Ⅱ , which is a crucial component for sun-light conversion and water split in PS Ⅱ. So study on its structure and function is very important. So far, a few structural models of P（680） have been proposed. Typical models are monomolecular model and bimolecular model. There are two types of bimolecular model: bimolecular parallel model and bimolecular angular model. (Note: There is a kind of multiple model. Because it can be obtained from the organization of two bimolecular models, this model is considered as the same sort of bi-

关键词： The structural model of P

The excitation energy transfer between β-Car and Chla molecules in PS Ⅱ core antenna complexes CP43 and CP47

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chinese Science Bulletin 2000年第17期45卷 1579-1583页

作者： SHAN Jixiu, WANG Jushuo, li liangbi,ZHAO Nanming & kuang Tingyun1. Laboratory of Photosynthesis Basic research, institute of botany, chinese academy of sciences, beijing 100093. china 2. Department of Biological sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, beijing 100084, chinaCorrespondence should be addressed to kuang Tingyun Laboratory of Photosynthesis Basic research Institute of Botany Chinese Academy of Sciences Beijing China Department of Biological sciences and Biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China

The excitation energy transfer efficiency between p-Car and Chla molecules in purified CP43 and CP47 was calculated by comparing absorption and fluorescence excitation after normalization at 550 nm, CP43 had an energy transfer efficiency of 29.1% while the CP47 had an energy transfer efficiency of 62.8%, proving that excitation energy was transferred between p-Car and Chla molecules in CP43 and CP47 at normal conditions. The excitation energy transfer between p-Car and Chla molecules in CP43 and CP47 may occur through the "Dexter" mechanism and the distance between these two kinds of pigments should be less than 1 nm. In addition, the results were also used to discuss the conformational relationship between p-Car and Chla molecules in CP43 and CP47.

关键词： CP43 CP47 β-Car Chla excitation energy transfer.

Mutagenesis of Ser^(24) of cytochrome b559 α subunit affects PSII activities in Chlamydomonas reinhardtii

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chinese Science Bulletin 2007年第7期52卷 896-902页

作者： MA JingJing li liangbi JING YuXiang kuang TingYun Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Graduate University of chinese academy of sciences Beijing 100049 China

In order to study the functions of cytochrome b559 (Cyt b559) in photosystem two (PSII) activity, mutant S24F of Chlamydomonas reinhardtii was constructed using site directed mutagenesis, in which Serine24 (Ser24) locating downstream of Histidine23 (His23) in α subunit of Cyt b559 was replaced by Phenylalanine (Phe). Physiological and biochemical analysis showed that mutant S24F could be grown photoautotrophically or photoheterotrophically. However, their growth rate was slower either on HSM or TAP medium than that of the control; Analysis of PSII activity revealed that its oxygen evolution was about 71% of wild type (WT); The Photochemical efficiency of PSII (Fv/Fm) of S24F was reduced 0.23 compared with WT; S24F was more sensitive to strong light irradiance than the wild type; Furthermore, SDS-PAGE and Western-blotting analysis indicated that the expression levels of α subunit of Cyt b559, LHCII and PsbO of S24F were a little less than those of the wild type. Overall, these data suggests that Ser24 plays a significant role in making Cyt b559 structure maintain PSII complex activity of oxygen evolution although it is not directly bound to heme group.

关键词：莱茵衣藻细胞色素b559α亚基丝氨酸残基 Ser24 突变光系统II 光合活性

Simulation for the primary electronic donor of photosystem II in vitro

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chinese Science Bulletin 2000年第10期45卷 914-920页

作者： PAN Jie ZHANG Chunxi GONG Yandao li liangbi ZHAO Nanming kuang Tingyun Photosynthesis research center Institute of BotanyChinese Academy of SciencesBeijing 100093China National Laboratory of Biomembranes and Membrane Biotechnology Department of Biological Science and BiotechnologyTsinghua UniversityBeijing 100084China Laboratory of Optical Physics Institute of PhysicsChinese Academy of SciencesBeijing 100080China

Histidine coordinated to Chl a is a distinct characteristic of Chl a in vivo. By usinghistidine analogue of 1-methylimidazole (C4H6N2) and measuring the UV/vis absorption, CD and MCD spectra of the interaction between C4H6N2and Chl a in CCI4, we have obtained that: (i) In pure CCI4 solvent, Chl a molecule is in five-coordinate state, and two Chl a molecules form an asymmetric compact-dimer with strong coupling interaction. We propose that the two Chl a molecules are connected by two unequally coordinated Mg-O bonds (the two oxygen atoms come from the C=O of C131 keto and C17 ester, respectively); (ii) when the molar ratio of C4H6N2/Chl a is 0.5 or 1 (corresponding to 2Chl a #1C4H6N2 and 2Chl a ·2C4H6N2, respectively), significant changes have been observed in the absorption, CD and MCD spectra, which indicate that the Chl a remains in dimer form, but the coupling interaction between them reduces greatly. We postulate that C4H6N2 replaces the ligation of C=O of C17 ester and C131 keto to Mg atoms

关键词： photosynthesis Chl a histidine coordination P680 simulation spectroscopy research.

Positional distribution of fatty acids on the glycerol backbone during the biosyn-thesis of glycerolipids in Ectocarpus fasciculatus

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chinese Science Bulletin 2002年第21期47卷 1802-1806页

作者： XU Yinong WANG Zeneng VAN Xiaojun liN Wei li liangbi kuang Tingyun Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Key Laboratory of Experimental Marine Biology Institute of Marine BiologyChinese Academy of SciencesQingdao 266071China

The biosynthesis of glycolipids in ***-tus was studied by 14C label and chase. The fatty acids in sulphoquinovosyl diacylglycerol (SQDG) were almost 16-car-bon and 18-carbon ones. In addition to the two fatty acids, monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) contained 8.5 mol% and 31.0 mol% of eicosapentaenoic acid (20 : 5), respectively, and this fatty acid was usually distributed in the sn-1 position of the glyc-erol backbone. When plants were incubated with [2-14C] acetate, differences existed in the positional distribution of the labeled fatty acids in sn-1 and sn-2 among the three glycerolipids. In SQDG, 14C-labeled fatty acids were distributed uniformly in the sn-1 and sn-2 positions. In DGDG, 14C-labeled fatty acids were mainly distributed in the sn-2 position. In MGDG, the radioactivity of fatty acids in sn-1 position was far greater than that in sn-2 position after a 30 min pulse label, and the difference in radioactivity between the two positions

关键词： E. fasciculatus glycerolipid fatty acid isotope label.

The photodamage process of pigments and proteins of PSI complexes from Spinacia Oleracea L.

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chinese Science Bulletin 2001年第21期46卷 1812-1816页

作者： WEI Jie YU Hui li liangbi kuang Tingyun WANG Jushuo GONG Yandao ZHAO Nanming Laboratory of Photosynthesis Basic research Institute of BotanyChinese Academy of SciencesBeijing 100093China Department of Biological Science and Biotechnology Tsinghua UniversityBeijing 100084 China

Purified PSI complexes from Spinacia Oleracea L. were exposed to the strong light (PFD=2300μmol m-2s-1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complexes was investigated using absorption, fluorescence, circular dichroism (CD) spectroscopy and SDS-PAGE. It was found from the optical absorption spectra that the maximal ab-sorbance of PSI complexes decreased and maximal peaks blue-shifted during the illumination, and the forth derivative spectra demonstrated that the absorbance decreasing at red region mainly resulted from the aborbance decreasing of the long wavelength Chla, implying that the long-wavelength Chla was readily to be bleached. The CD signals contributed by LHCI decreased more rapidly than other CD signals con-tributed by Chla and Carotenoid, indicating that the LHCI was more sensitive to light than core complexes. It was observed by SDS-PAGE that some small polypeptides of PSI complexes were damaged earlier than reaction

关键词： PSI pigment-protein complex photodamage LHCI absorption spectra CD spectra.