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Depletion of phosphatidyl-glycerol head-group induces changes in oxygen evolution and protein secondary structures of photosystem Ⅱ

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chinese Science Bulletin 2002年第10期47卷 824-829页

作者： yang zhenle, wang zeneng, jiang guizhen, xu yinong, li liangbi & kuang tingyunphotosynthesis research center, institute of botany, chinese academy of sciences, beijing 100093. china Photosynthesis research center Institute of Botany Chinese Academy of Sciences Beijing China

The techniques of oxygen electrode polarogra-phy and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and structural aspects of photosystem Ⅱ (PS Ⅱ) through enzymatic approach. It was shown that the depletion of PG by treatment of phospholipase C (PLC) on PS Ⅱ particles caused the inhibition of oxygen evolving activity in PS Ⅱ. This effect also gave rise to changes in the protein secondary structures of PS Ⅱ, that is, an increase in a-helical conformation which is compensated by the loss of p-strand structures. It revealed that the head-group of PG molecules plays an important structural role in the maintenance of normal structure of PS Ⅱ proteins, which is required to maintain the appropriate physiological activity of the PS Ⅱ complex such as the oxygen evolving activity. It is suggested that there most probably exist hydrogen-bonding interactions between PG molecules and PS Ⅱ proteins.

关键词： photosystem II polar head-group of phosphatidylglyc-erol phospholipase C oxygen evolution protein secondary struc-ture.

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Positional distribution of fatty acids on the glycerol backbone during the biosyn-thesis of glycerolipids in Ectocarpus fasciculatus

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chinese Science Bulletin 2002年第21期47卷 1802-1806页

作者： xu yinong wang zeneng VAN Xiaojun liN Wei li liangbi kuang Tingyun Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Key Laboratory of Experimental Marine Biology Institute of Marine BiologyChinese Academy of SciencesQingdao 266071China

The biosynthesis of glycolipids in ***-tus was studied by 14C label and chase. The fatty acids in sulphoquinovosyl diacylglycerol (SQDG) were almost 16-car-bon and 18-carbon ones. In addition to the two fatty acids, monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) contained 8.5 mol% and 31.0 mol% of eicosapentaenoic acid (20 : 5), respectively, and this fatty acid was usually distributed in the sn-1 position of the glyc-erol backbone. When plants were incubated with [2-14C] acetate, differences existed in the positional distribution of the labeled fatty acids in sn-1 and sn-2 among the three glycerolipids. In SQDG, 14C-labeled fatty acids were distributed uniformly in the sn-1 and sn-2 positions. In DGDG, 14C-labeled fatty acids were mainly distributed in the sn-2 position. In MGDG, the radioactivity of fatty acids in sn-1 position was far greater than that in sn-2 position after a 30 min pulse label, and the difference in radioactivity between the two positions

关键词： E. fasciculatus glycerolipid fatty acid isotope label.

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