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Effect of Directional Stretching on Properties of PVA-HA-PAA Composite Hydrogel

Journal of Bionic Engineering 2021年第5期18卷 1202-1214页

作者： Kai Chen Qin Chen Tian Zong Siyu Liu xuehui yang Yong Luo Dekun Zhang School of Materials Science and Physics China University of Mining and TechnologyXuzhou 221116China Department of Mechanical Engineering Purdue School of Engineering and TechnologyIUPUIIndianapolisIN 46202USA

Polyvinyl alcohol(PVA)hydrogels with excellent characteristics are considered as promising cartilage replacement ***,there are still some main issues to be solved for PVA hydrogel,such as poor mechanical strength and disordered *** by the highly ordered structure of biological soft tissues such as articular cartilage,here,we prepared a high-strength but anisotropic polyvinyl alcohol-nanohydroxyapatite-polyacrylic acid(PVA-HA-PAA)composite hydrogel by directional stretching,freezing–thawing,and annealing *** of an as-prepared isotropic PVA-HA-PAA composite hydrogel leads to the orientation of PVA crystallites and PVA chains,which enables the formation of ordered structure and more hydrogen bonds via freezing under *** microstructure,water content,swelling and creep performance,tensile and bio-tribology properties of the composite hydrogel are studied,the results indicated that the properties of the hydrogel are aff ected by stretching due to the formation of ordered structure in the anisotropic *** instance,the elastic modulus and tensile strength of the anisotropic hydrogel reach 5.703 MPa and 18.958 MPa,respectively,which is significantly enhanced by comparing with isotropic ***,the friction property is anisotropic,and the Coefficient Of Friction(COF)reduced in the parallel ***,this work provides a simple and practicable strategy to design strong and anisotropic hydrogels for potential applications in biomedical materials such as cartilage substitute.

关键词： PVA-HA-PAA hydrogel Directional stretching Anisotropic Mechanical properties Friction property

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Research on the Characteristics of Anomalies in ELF Electromagnetic Observations in Yunnan Province

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Earthquake Research in China 2013年第2期27卷 269-281页

作者： Sun Weihuai Tang Ji Zhang Ping yang xuehui Tonghai Seismic Station Earthquake Administration of Yunnan Province Institute of Geology China Earthquake Administration Precursory Observation Network Earthquake Administration of Yunnan Province

Located in an earthquake-prone region,the geological structures in Yunnan Province are complex. Taking into account that Tonghai county is located in the intersection of Xiaojiang fault and Honghe fault, an ACF-4M ELF electromagnetic instrument was installed at the Tonghai seismic station,which has produced continuous reliable data. The author collected the data and information for the year 2009 and 2010,and performed analysis on the variation characteristics of both geomagnetic fields and electrical resistivity. The result shows that the 1Hz and 39Hz electromagnetic power spectra are 0. 2 to 1. 4 orders of magnitude higher than the normal values immediately before many earthquakes. The anomalies are represented by the abrupt changes of the electric and magnetic field power spectra in earthquake and aftershock sequences,and the amplitude of change is related to the size of earthquake magnitude and epicentral distance. The electrical resistivity also obviously changes. Therefore, further research on the anomalous characteristics of ELF electromagnetic data will be meaningful to the future use of this instrument in earthquake prediction.

关键词： ELF Electromagnetic Power spectrum Apparent resistivity Short-impending anomaly

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The effects of the same target on malignant proliferation of human lung cancer cells with different expression levels of COX-2 protein

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The Chinese-German Journal of Clinical Oncology 2010年第3期9卷 125-132页

作者： Weiying Li Hui Wang Baitang Lai xuehui yang Chunyan Zhang Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing 101149 China

Objective: The aim of the study was to explore the effects of the same target (si-10) on lung cancer cells with different expression levels of cyclooxygenase-2 (COX-2) protein by RNAi and malignant proliferation of these cells. Methods: COX-2 was selected as the target and one siRNA expression vector with the best effect was selected and thought as the subject from three COX-2 siRNA expression vectors with human U6 promoter. The siRNA expression vector (psi-10) and the vacant vector (pEGFP) were transfected into these cells with different COX-2 expression states (801D, A549 and LTEP-A2) with lipofectamine respectively and the transfected cell strains were constructed. The change of COX-2 expression levels was examined by Western blot and RT-PCR. The effects on the proliferation of lung cancer cells were studied by cell growth curve and clonogenic assay. Results: The siRNA and U6 promoter were validated by PCR, restriction endonucleases identification and DNA sequencing and BLAST alignment and cloned into the pEGFP vector. The cell strains transfected that 801D was used as maternal line were named as 801D-p and 801D-10 respectively. The cell strains transfected that A549 was used as maternal line were named as A549-p and A549-10 respectively. The cell strains transfected that LTEP-A2 was used as maternal line were named as LTEP-A2-p and LTEP-A2-10 respectively. These cells transfected pEGFP (801D-p, A549-p and LTEP-A2-p) had the expression of GFP and 801D-10, A549-10 and LTEP-A2-10 cells had not in 24, 48 and 72 hours after transfected. The results of RT-PCR and Western blot showed the siRNA expression vector produced marked effects in two cells (A549 and LTEP-A2) expressing COX-2 and the expression of COX-2 was inhibited. But the inhibited effects were differ- ent and the expression of COX-2 was more inhibited obviously in LTEP-A2 cells than in A549 cells though the expression of COX-2 was also inhibited obviously in A549 cells. In contract to their maternal line, t

关键词： cyclooxygenase-2 (COX-2) lung cancer cells RNAi malignant proliferation

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The effects of interfering in COX-2 gene expression on malignant proliferation of the human lung adenocarcinoma A2 cell in vitro

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The Chinese-German Journal of Clinical Oncology 2009年第9期8卷 498-503页

作者： Weiying Li Hui Wang Baitang Lai xuehui yang Chunyan Zhang Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing 101149 China

Objective:We explored the interfering effect of COX-2 gene expression and the influence on the malignant proliferation of A2 cells by RNAi after quenching COX-2 in vitro. Methods:COX-2 was selected as the subject. Three COX-2 siRNA expression vectors with human U6 promoter were constructed and three vectors and the vacant vector (pEGFP) were transfected into A2 cells with lipofectamine respectively. The cell strains transfected were constructed. The change of COX-2 expression levels was examined by Western blot and RT-PCR. The effects on the proliferation of A2 cells after silencing COX-2 were studied by cell growth curve and clonogenic assay in vitro. Results: The three siRNA and U6 promoter cloned into pEGFP plasmid were validated by PCR, restriction endonucleases identification, DNA sequencing and BLAST alignment. The cell strains transfected were named as A2-3, A2-7, A2-10 and A2-p respectively. Green fluorescence was seen in A2-p cells and not in A2-3, A2-7 and A2-10 cells in 24, 48 and 72 h after transfected. The results of RT-PCR and Western blot showed the three siRNA expression vectors produced effects and the expression of COX-2 was inhibited in different extent. In contrast to A2 cells, the levels of COX-2 mRNA of A2-3, A2-7 and A2-10 cells reduced 15.6%, 20.4% and 64.2% respectively; the levels of COX-2 protein of A2-3, A2-7 and A2-10 cells reduced 23.7%, 36.7% and 60.2% respectively. The results of cell growth curve and clonogenic assay showed the growth of A2-10 cell slowed and the colonial formation rate reduced but the growth of A2-3 and A2-7 cells had not obvious changes in contrast to the controls (A2 and A2-p). Conclusion: Silencing the COX-2 gene in vitro by RNAi technique can significantly inhibit the malignant proliferation of A2 cells.

关键词： COX-2 RNAi malignant proliferation in vitro

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Control Effect of Trichoderma harzianum against Potato Early Dying in China

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Plant Diseases and Pests 2013年第4期4卷 17-19,24页

作者： Chen Xiaojun Yuan Jie Sopone Wongkaew Wu Shiping He Haiyong yang xuehui Natthiya Buensanteai School of Crop Production Technology of Agriculture Technology Suranaree University of Technology Guizhou Institute of Plant Protection Guizhou Technological Center for Pollution-free Plant Protection Engineering School of Crop Production Technology of Agriculture Technology Suranaree University of TechnologyNakhon Ratchasima 30000Thailand

[Objective] The paper was to explore the optimum dosage and application pattern of Trichoderma harzianum for controlling Potato Verticillium wilt. [ Method] Two trials, sowing seed tubers in bacterial manure, sowing seed tubers in bacterial manure ＋ root irrigation after seedling emergence, were conducted in the paper. [Result] *** could effectively control potato early dying and promote potato growth, thereby increasing potato yield. The control effects of sowing tuber in 300 times of bacterial manure ＋ root irrigation with 300 times of conidia suspension after seedling emergence were ideal ; the highest control effect and the highest saved yield loss were 72.60% and 33.33%, respectively. [ Conclusion] The paper provides reference for field application of T. harzianum.

关键词： Trichoderma harzianum Potato early dying

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Isolation Technology of Single Spore and Optimization of Conidia Culture Condition for Ustilaginoidea virens

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Plant Diseases and Pests 2012年第5期3卷 21-23页

作者： Haiyong HE Qingqun TAN Xiaojun CHEN xuehui yang Shiping WU Lishuang WANG Wongkaew Sopone Jie YUAN Guizhou Institute of Plant Protection School of Crop Production Technology Institute of Agricultural TechnologySuranaree University of TechnologyNakon 30000Thailand

The single spores were isolated from chlamydospores of Ustilaginoidea virens with three different maturities by PSA. The isolated single spores were cul- tured on different media at different temperatures under natural light for inducing conidia to explore the optimum isolation technique of single spore and optimum cul- ture condition of conidia. The results showed that the successful isolating rate of single spore from yellow rice false smut balls reached 90.00%. The sporulatina quantities of isolated single spores cultured on PSD and PDB media at 22 -29 ~C （variable temperature under natural light） or 28 ℃ （constant temperature under dark condition） for 12 d were up to 6.3× 107 and 1.1× 106 spore/mL, respectively. PSA was the most effective method to isolate single spores from yellow rice false smut balls of U. virens. The optimum conidia culture condition included PSD or PDB medium, 22 -29℃ or 28℃, natural light and vibration culture. Key words Ustilaginoidea virens; Single spore isolation; Conidia; Culture condition

关键词： Ustilaginoidea virens Single spore isolation Conidia Culture condition

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杭州亚运档案资源体系建设探究

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档案学通讯 2023年第6期 44-51页

作者：胡广成杨雪辉杭州远大档案技术有限公司杭州310005 杭州市档案馆杭州310024

杭州亚运档案作为杭州亚运会全周期的真实记录,是杭州亚运会的宝贵遗产,具有重要的历史和文化价值。厘清杭州亚运档案收集范围、资源体系建设内容,是开展亚运档案资源体系建设的必要前提。杭州亚运档案具有形成主体众多、领域涉及广泛... 详细信息

杭州亚运档案作为杭州亚运会全周期的真实记录,是杭州亚运会的宝贵遗产,具有重要的历史和文化价值。厘清杭州亚运档案收集范围、资源体系建设内容,是开展亚运档案资源体系建设的必要前提。杭州亚运档案具有形成主体众多、领域涉及广泛、时间跨度长、地区分布广的鲜明特点。在梳理杭州亚运会申办、筹办、赛时、“后亚运时代”各阶段所形成的档案基础上,提出多措并举确保杭州亚运档案收集齐全完整、合理区分杭州亚运档案归属、统一杭州亚运档案管理规范、建立杭州亚运档案专题数据库,以探究构建全要素、全生命周期的大亚运档案资源体系的路径。

关键词：杭州亚运会亚运档案档案资源体系

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激光选区熔化高强铝合金热行为模拟研究

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激光与光电子学进展 2022年第23期59卷 179-185页

作者：杨雪慧张争艳王顺河北工业大学机械工程学院天津300130

针对高强铝合金利用选区激光熔化技术成形困难的问题,基于ANSYS软件进行有限元分析研究激光选区熔化变形铝合金,揭示了成形过程中温度场分布与基本特征,并通过温度分布云图研究了扫描道的温度梯度变化。同时,研究了激光功率和扫描速度... 详细信息

针对高强铝合金利用选区激光熔化技术成形困难的问题,基于ANSYS软件进行有限元分析研究激光选区熔化变形铝合金,揭示了成形过程中温度场分布与基本特征,并通过温度分布云图研究了扫描道的温度梯度变化。同时,研究了激光功率和扫描速度对熔池热行为的影响,借助仿真研究了扫描工艺参数对熔池形态变化的影响规律。结果发现:在仿真成形过程中,熔池尺寸与扫描速度呈非线性变化关系,与激光输入的线能量密度呈正相关,随着能量密度的增加,尺寸增加。通过试验对该模拟结果进行验证,此研究可以为后续的成形分析提供理论参考。

关键词：激光光学选择性激光熔化数值模拟高强铝合金熔池形态热行为

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预应力带肋板与叠合板抗弯性能试验

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沈阳建筑大学学报（自然科学版） 2017年第1期33卷 77-85页

作者：孟宪宏沙连凯佟林杨学会沈阳建筑大学土木工程学院辽宁沈阳110168 沈阳市城市基础设施建设投资发展有限公司辽宁沈阳110013

目的研究预应力带肋板和预应力带肋叠合板的抗弯受力性能,为在实际工程中的应用以及数值模拟分析提供参考依据.方法采用自行设计的预应力带肋板与叠合板,分别对预应力带肋板与预应力带肋叠合板进行均布加载试验,并采用ABAQUS软件进行模... 详细信息

目的研究预应力带肋板和预应力带肋叠合板的抗弯受力性能,为在实际工程中的应用以及数值模拟分析提供参考依据.方法采用自行设计的预应力带肋板与叠合板,分别对预应力带肋板与预应力带肋叠合板进行均布加载试验,并采用ABAQUS软件进行模拟与试验数据对比,通过试验数据、试验现象分析力学性能.结果净跨4 640 mm的预应力(肋宽:120 mm、肋高:70 mm)带肋板在预应力度λ=0.47,施工荷载2 k Pa作用时,施工期间不需要加设支撑,可以满足承载力、挠度和变形要求;预应力带肋叠合板的最大跨度通过计算可以达到5 100 mm.预应力带肋叠合板的挠度、裂缝在达到使用荷载时均满足要求.预应力带肋叠合板的结合面没有出现起皮、错层现象.结论新型的预应力带肋叠合板具有制作工序简单、整体性能良好的优点,且板上所凸起的肋取代了常见叠合板结构中的桁架筋,既节约了大量钢筋,又能够满足施工过程中的吊装要求.

关键词：预应力带肋板预应力带肋叠合板抗弯性能数值模拟分析

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白细胞介素-17在骨代谢与炎症性骨疾病中的作用

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遵义医学院学报 2016年第5期39卷 441-448页

作者：杨学辉美国缅因医学研究中心研究所分子医学部

骨稳态取决于破骨细胞的溶骨活性与成骨细胞的成骨作用之间的相对平衡。在慢性炎症情况下,如风湿性关节炎、牙周炎、银屑病等,由于炎性因子的作用致使破骨细胞溶骨作用相对增强打破骨代谢平衡,导致骨溶解丢失。白细胞介素-17(IL-17)是... 详细信息

骨稳态取决于破骨细胞的溶骨活性与成骨细胞的成骨作用之间的相对平衡。在慢性炎症情况下,如风湿性关节炎、牙周炎、银屑病等,由于炎性因子的作用致使破骨细胞溶骨作用相对增强打破骨代谢平衡,导致骨溶解丢失。白细胞介素-17(IL-17)是近年来发现的新型细胞因子家族,参与机体多种免疫防御。研究发现,IL-17可影响成骨细胞和破骨细胞的增殖与分化,对骨代谢有着重要调节作用。深入了解IL-17在骨代谢中的生物学作用有助于探寻治疗炎症性骨疾病的有效方案,本文就IL-17对正常骨代谢调节及病理情况下与骨疾病发生发展的关系作一综述。

关键词：白细胞介素-17(IL-17) IL-17受体(IL-17R) RANKL 破骨细胞成骨细胞炎症性骨疾病

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