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HAL1 mediate salt adaptation in Arabidopsis thaliana

Cell research 2001年第2期11卷 142-148页

作者： yang su xin, yan xiu zhao, quan zhang, yu ke he, hui zhang, da luo institute of plant physiology, shanghai institutes for biological sciences. chinese academy of science, 300 fenglin road, shanghai 2.0032. china key laboratory of plant stress research, sh 1institute of plant physiology Shanghai Institutes for Biological Sciences Chinese Academyof Science 300 Fenglin Road Shanghai 200032 China 2key laboratory of plant stress research Shandong Normal University Jinan 250014 China 2key laboratory of plant stress research Shandong Normal University Jinan 250014 China 1institute of plant physiology Shanghai Institutes for Biological Sciences Chinese Academyof Science 300 Fenglin Road Shanghai 200032 China 2key laboratory of plant stress research Shandong Normal University Jinan 250014 China 1institute of plant physiology Shanghai Institutes for Biological Sciences Chinese Academyof Science 300 Fenglin Road Shanghai 200032 China

The yeast HAL1 gene was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation with vacuum infiltration under the control of CaMV 35S promoter. Thirty-three individual kanamycin resistant plants were obtained from 75,000 seeds. Southern blotting analysis indicated that HAL1 gene had been integrated into all of the transgenic plants’ genomes. The copy number of HAL1 gene in transgenic plants was mostly 1 to 3 by Southern analysis. Phenotypes of transgenic plants have no differences with wild type plants. several samples of transformants were self-pollinated, and progenies from transformed and non-transformed plants (controls) were evaluated for salt tolerance and gene expression. Measurement of concentrations of intracellular K+ and Na+ showed that transgenic lines were able to retain less Na+ than that of the control under salt stress. Results from different tests indicated the expression of HAL1 gene promotes a higher level of salt tolerance in vivo in the transgenic Arabidopsis plants.

关键词： Salt-tolerant Arabidopsis HAL1 gene engineering.

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