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Quantification of microcystin-producing and non-microcystin producing Microcystis populations during the 2009 and 2010 blooms in Lake Taihu using quantitative real-time PCR

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Journal of Environmental Sciences 2012年第2期24卷 284-290页

作者： Daming Li Fanxiang Kong Xiaoli Shi Linlin Ye yang Yu zhen yang State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and Limnology Chinese Academy of Sciences Nanjing 210008 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

Lake Taihu,a large,shallow hypertrophic freshwater lake in eastern China,has experienced lake-wide toxic cyanobacterial blooms annually during summer season in the past *** changes in the abundance of hepatotoxin microcystin-producing and nonmicrocystin producing Microcystis populations were investigated in the lake in August of 2009 and *** monitor the densities of the total Microcystis population and the potential microcystin-producing subpopulation,we used a quantitative real-time PCR assay targeting the phycocyanin intergenic spacer（PC-IGS） and the microcystin synthetase gene（mcyD）,*** the basis of quantification by real-time PCR analysis,the abundance of potential toxic Microcystis genotypes and the ratio of the mcyD subpopulation to the total Microcystis varied significantly,from 4.08×104 to 5.22×107 copies/mL,from 5.7% to 65.8%,*** analysis showed a strong positive relationship between chlorophyll-a,toxic Microcystis and total Microcystis;the abundance of toxic Microcystis correlated positively with total phosphorus and ortho-phosphate concentrations,but negatively with TN：TP ratio and nitrate *** the proportion of potential toxic genotypes within Microcystis population showed positive correlation with total phosphorus and ortho-phosphate *** data suggest that increased phosphorus loading may be a significant factor promoting the occurrence of toxic Microcystis bloom in Lake Taihu.

关键词： microcystin-producing Microcystis real-time PCR toxic cyanobacterial bloom microcystin synthetase gene cluster

Effects of Ni Content and Ball Milling Time on the Hydrogen Storage Thermodynamics and Kinetics Performances of La–Mg–Ni Ternary Alloys

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Acta Metallurgica Sinica(English Letters) 2019年第8期32卷 961-971页

作者： zhen-yang Li Sheng-Li Li Ze-Ming Yuan yang-Huan Zhang School of Materials Science and Engineering Shandong UniversityJinan 250061China Key Laboratory of Integrated Exploitation of Baiyun Obo Multi-Metal Resources Inner Mongolia University of Science and TechnologyBaotou 014010China Department of Functional Material Research Central Iron and Steel Research InstituteBeijing 100081China

The eifects of Ni content and ball milling time on the hydrogen storage thermodynamics and kinetics performances of asmilled La5Mg95-xNix(x=5,10,15)ternary alloys have been *** evolution of microstructure and phase of experimental alloys in the absorption/desorption process has been characterized by XRD,SEM and *** hydrogen storage kinetics and thermodynamics performances and PCI curves have been tested using the Sievert *** is found that the rising of Ni content remarkably improves the hydrogen storage kinetic performance,but reduces hydrogen storage *** with the increase in milling time,hydrogen desorption activation(Ea)value decreases firstly and then increases;the minimum value is 47.6 kJ/mol,and the corresponding milling time is 10 h for La5Mg85Ni10 *** for the thermodynamics properties,the hydrogenation enthalpy(△H)and hydrogenation entropy(△S)both decrease firstly and then increase with the rising of Ni content and milling *** composite La5Mg85Ni10 alloy milled for 10 h exhibits the best thermodynamics and kinetics performances,the lowest Ea of 47.6 kJ/mol,absorption of 5.4 wt.%within 5 min and desorption of 5.2 wt.%within 3 min at 360℃and the lowest△H and△S of 72.1 kJ/mol and 123.2 J/mol/K.

关键词： Hydrogen storage Mg-based alloys Thermodynamics and kinetics Ni content Milling time

A New Approach for Controlling Chaos Based on Direct Optimizing Predictive Control

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Communications in Theoretical Physics 2006年第3期45卷 473-476页

作者： LI Dong-Mei yang Shao-zhen School of Economy and Management Hebei University of Science and Technology Shijiazhuang 050018 China

We introduce the predictive control theory into predictive control algorithm based on a neural network model the study of chaos control and propose a direct optimizing The proposed control system stabilizes the chaotic motion in an unknown chaotic system onto the desired target trajectory. Compared with the existing similar algorithms, the proposed control system has faster response, so it requires much shorter time for the stabilization of the chaotic systems. The proposed approach can control hyperchaos and the algorithm is simple. The convergence of the control algorithm and the stability of the control system can be guaranteed. The theoretic analysis and simulations demonstrate the effectiveness of the algorithm.

关键词： vchaos control predictive control chaotic system neural networks

An Alternative Scheme for Transferring Quantum States and Preparing a Quantum Network in Cavity QED

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Communications in Theoretical Physics 2007年第6期47卷 1037-1040页

作者： yang zhen-Biao SU Wan-Jun Department of Electronic Science and Applied Physics Fuzhou University Fuzhou 350002 China

An alternative scheme is proposed to transfer quantum states and prepare a quantum network in cavity QED. It is based on the interaction of a two-mode cavity field with a three-level V-type atom. In the scheme, the atom-cavity field interaction is resonant, thus the time required to complete the quantum state transfer process is greatly shortened, which is very important in view of decoherence. Moreover, the present scheme does not require one mode of the cavities to be initially prepared in one-photon state, thus it is more experimentally feasible than the previous ones.

关键词： quantum states transfer quantum network cavity QED

Effects of elevated CO_2 on dynamics of microcystin-producing and non-microcystin-producing strains during Microcystis blooms

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Journal of Environmental Sciences 2015年第1期27卷 251-258页

作者： Li Yu Fanxiang Kong Xiaoli Shi zhen yang Min Zhang yang Yu State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and Limnology Chinese Academy of Sciences University of Chinese Academy of Sciences

In an attempt to elucidate the effects of different CO2concentrations（270, 380, and 750 μL/L） on the competition of microcystin-producing（MC-producing） and non-MC-producing Microcystis strains during dense cyanobacteria blooms, an in situ simulation experiment was conducted in the Meiliang Bay of Lake Taihu in the summer of 2012. The abundance of total Microcystis and MC-producing Microcystis genotypes was quantified based on the 16 S r DNA and mcy D gene using real-time PCR. The results showed that atmospheric CO2 elevation would significantly decrease the p H value and increase the dissolved inorganic carbon（DIC） *** in CO2 concentration did not show significant influence on the abundance of total Microcystis population. However, CO2 concentrations may be an important factor in determining the subpopulation structure of Microcystis. The enhancement of CO2 concentrations could largely increase the competitive ability of non-MC-producing over MC-producing Microcystis, resulting in a higher proportion of non-MC-producing subpopulation in treatments using high CO2 concentrations. Concurrently, MC concentration in water declined when CO2 concentrations were elevated. Therefore, we concluded that the increase of CO2 concentrations might decrease potential health risks of MC for human and animals in the future.

关键词： Microcystis Microcystin Inorganic carbon Real-time PCR Harmful cyanobacterial blooms

Exosome-mediated transfer of circRNA563 promoting hepatocellular carcinoma by targeting the microRNA148a-3p/metal-regulatory transcription factor-1 pathway

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World Journal of Gastroenterology 2023年第46期29卷 6060-6075页

作者： Zhuo-zhen Lyu Meng Li Ming-Yu yang Mei-Hong Han zhen yang Department of Infectious Diseases Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina

BACKGROUND Mesenchymal stem cells(MSCs)exert anti-oncogenic effects via exosomes containing non-coding RNA(ncRNA),which play important roles in tumor *** preliminary study identified the interaction of the ncRNA hsa_-circ_0000563(circ563)and the circ563-associated miR-148a-3p in exosomes,as miR-148a-3p and its target metal-regulatory transcription factor-1(MTF-1)are implicated in hepatocellular carcinoma(HCC)*** To identify the clinical significance,functional implications,and mechanisms of circ563 in *** The expression levels of miR-148a-3p and MTF-1 in exosomes derived from MSC and HCC cells were compared,and their effects on HCC cells were *** a dual-luciferase reporter assay,miR-148a-3p was identified as an associated microRNA of circ563,whose role in HCC regulation was assessed in vitro and in *** The silencing of circ563 blocked the HCC cell proliferation and invasion and induced ***-culturing of HCC cells with MSC-derived exosomes following circ563 overexpression promoted cell proliferation and metastasis and elicited changes in miR-148a-3p and MTF-1 *** tumor-promoting effects of circ563 were partially suppressed by miR-148a-3p overexpression or MTF-1 *** experiments performed in nude mice confirmed that circ563-enriched exosomes facilitated tumor growth by upregulating the expression of *** HCC tissues,circ563 expression was negatively correlated with miR-148a-3p expression but positively correlated with MTF-1 *** MSCs may exhibit anti-HCC activity through the exosomal circ563/miR-148a-3p/MTF-1 pathway,while exosomes can transmit circ563 to promote oncogenic behavior by competitively binding to miR-148a-3p to activate MTF-1.

关键词： Exosome Cell communication Noncoding RNA Metal-regulatory transcription factor-1 Mesenchymal stem cells

Sum-Rate Maximization in Active RIS-Assisted Multi-Antenna WPCN

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China Communications 2024年第6期21卷 23-39页

作者： Jiang Jie Lyu Bin Chen Pengcheng yang zhen School of Communications and Information Engineering Nanjing University of Posts and TelecommunicationsNanjing 210003China Jiangsu Provincial Key Laboratory of Photonic and Electronic Materials Sciences and Technology Nanjing UniversityNanjing 210003China

In this paper,we propose an active reconfigurable intelligent surface(RIS)enabled hybrid relaying scheme for a multi-antenna wireless powered communication network(WPCN),where the active RIS is employed to assist both wireless energy transfer(WET)from the power station(PS)to energyconstrained users and wireless information transmission(WIT)from users to the receiving station(RS).For further performance enhancement,we propose to employ both transmit beamforming at the PS and receive beamforming at the *** formulate a sumrate maximization problem by jointly optimizing the RIS phase shifts and amplitude reflection coefficients for both the WET and the WIT,transmit and receive beamforming vectors,and network resource *** solve this non-convex problem,we propose an efficient alternating optimization algorithm with the linear minimum mean squared error criterion,semidefinite relaxation(SDR)and successive convex approximation ***,the tightness of applying the SDR is *** results demonstrate that our proposed scheme with 10 reflecting elements(REs)and 4 antennas can achieve 17.78%and 415.48%performance gains compared to the single-antenna scheme with 10 REs and passive RIS scheme with 100 REs,respectively.

关键词： active reconfigurable intelligent surface beamforming sum-rate maximization wireless powered communication network

Robust Generation of Qutrit-Qutrit Entanglement through a Single Resonant Interaction Assisted by Moderate Driving

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Chinese Physics Letters 2013年第12期30卷 49-53页

作者： WU Huai-Zhi yang zhen-Biao Lab of Quantum Optics Department of PhysicsFuzhou UniversityFuzhou 350002

We propose to engineer qutrit-qutrit entanglement through resonant atom-cavity interaction assisted by moderate laser driving,study two parameter regimes,respectively,of asymmetric atom-laser coupling and of asymmetric atom-cavity coupling,and find that both the coupling regimes possess the advantage of short operation time,compared with the previous ones achieved by dispersive interaction,adiabatic passage,and even quantum Zeno *** check numerically the influences of the parameter fluctuations and dissipation on the scheme and show it to be *** scheme can also be generalized to other physical systems such as the ion trap.

关键词： dynamics coupling scheme

Chemical reprogramming:the CiPSCs and the CiNs

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National Science Review 2017年第1期4卷 7-10页

作者： Xiang Li yang yang zhen yang Hongkui Deng The MOE Key Laboratory of Cell Proliferation and Differentiation College of Life SciencesPeking-Tsinghua Center for Life SciencesPeking University Department of Cell Biology School of Basic Medical SciencesPeking University Stem Cell Research CenterCenter for Molecular and Translational MedicineState Key Laboratory of Natural and Biomimetic DrugsPeking University Health Science Center Shenzhen Stem Cell Engineering Laboratory Key Laboratory of Chemical GenomicsPeking University Shenzhen Graduate School Laboratory of Chemical Genomics School of Chemical Biology and BiotechnologyPeking University Shenzhen Graduate School Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education and Beijing National Laboratory for Molecular Science(BNLMS)Peking-Tsinghua Center for Life SciencesPeking University Key Laboratory of Marine Drugs Chinese Ministry of EducationSchool of Medicine and PharmacyOcean University of China

TRANSGENIC APPROACH TO INDUCE CELL FATE REPROGRAMMING To generate desirable cells and to restore biological function,manipulating somatic cell fates is one of fundamental scientiic goals in regenerative *** study of Myod-converted myoblasts in 1987 demonstrated the concept to convert cell fates by introducing a single transcription factor[1].he development of induced pluripotent stem cells(i PSCs)demonstrated the feasibility of using a combination of

关键词： PSCs Chemical reprogramming:the CiPSCs and the CiNs cell