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RT-qPCR normalization of reference genes in different lifehistory stages of Gracilaria vermiculophylla(Rhodophyta)

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Journal of Oceanology and Limnology 2023年第5期41卷 1910-1917页

作者： Yingyue ZHANG Jinxin yang ze yang Cong QI Di XU Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China

The quantitative realtime polymerase chain reaction(RT-qPCR)is a powerful and sensitive method to measure expression of targeted gene but it highly relies on the use of suitable reference genes for data *** evaluated the expressions of 8 housekeeping genes:18S ribosomal rDNA(18S rDNA),28S ribosomal r DNA(28S rDNA),rubisco large subunit(rbc-L),β-actin(ACT),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),elongation factor 1(EF1),β-tubulin(Tub B),and P-phycoerythrin B(PEB),to select the suitable reference genes for different life-history stages(tetrasporophyte,carposporophyte,and male/female gametophyte)of Gracilaria vermiculophylla by absolute quantitative *** geNorm and BestKeeper were used to verify the results acquired from copy number *** show that the expression of identified reference genes varied in comparing groups composed of different type of life *** is suggested that 18S rDNA and TubB could be used for highly complex samples composed of mixed ploidy and phases.18S rDNA and 28S rDNA were also preferred for using among the matured isomorphic *** for samples with different maturities,TubB and ACT were recommended for tetrasporophytes and gametophytes respectively.

关键词： reference gene Gracilaria vermiculophylla life-history stage quantitative real-time polymerase chain reaction(RT-qPCR) red algae

Large-scale model test study on the water pressure resistance of construction joints of karst tunnel linings

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Frontiers of Structural and Civil Engineering 2023年第8期17卷 1249-1263页

作者： Meng HUANG Mingli HUANG ze yang Yuan SONG Zhien ZHANG School of Civil Engineering Beijing Jiaotong UniversityBeijing 100044China Enterprise Key Laboratory Tianjin Municipal Engineering Design and Research InstituteTianjin 300392China School of Civil Engineering and Architecture Anhui University of Science and TechnologyHuainan 232001China

Model tests and numerical calculations were adopted based on the New Yuanliangshan tunnel project to investigate the water pressure resistance of lining construction joints in high-pressure and water-rich karst tunnels.A large-scale model test was designed and conducted,innovatively transforming the external water pressure of the lining construction joint into internal water *** effects of the embedded position and waterstop type on the water pressure resistance of the construction joint were analyzed,and the reliability of the model test was verified via numerical *** results show that using waterstops can significantly improve the water pressure resistance of lining construction *** water pressure resistance of the lining construction joint is positively correlated with the lining thickness and embedded depth of the *** addition,the type of waterstop significantly influences the water pressure resistance of lining construction *** test results show that the water pressure resistance of the embedded transverse reinforced waterstop is similar to that of the steel plate waterstop,and both have more advantages than the rubber *** water pressure resistance of the construction joint determined via numerical calculations is similar to the model test results,indicating that the model test results have high accuracy and *** study provides a reference for similar projects and has wide applications.

关键词： karst tunnel lining construction joint water pressure resistance large-scale model test numerical calculations

Numerical Simulation of Dilute Phase Transportation in Pulse Riser

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China Petroleum Processing & Petrochemical Technology 2021年第4期23卷 130-140页

作者： An Xiaoxi yang ze Qiao Yingyun Tian Yuanyu Key Laboratory of Low Carbon Energy and Chemical Engineering Shandong University of Science and TechnologyQingdao 266590 State Key Laboratory of Heavy Oil Processing China University of PetroleumQingdao 266580

To study the hydrodynamic characteristics of gas-solid-phase flow in a pulse riser,a dense discrete particle model considering particle collisions and solid volume fraction is *** core-annular flow in the enlarged-diameter section of the pulse riser is described,which can be destroyed with a high superficial gas *** particle trajectory crossing effect and particle-particle and particle-wall collisions in the reduced-diameter section of the pulse riser can also destroy the core-annular flow and enhance the gas-solid *** solid volume fraction exhibits an S-type distribution at different solid mass *** distribution of axial velocity,radial velocity,and relative slip velocity is investigated by analyzing the simulated results at different pulse riser diameter ratios.A suitable pulse riser diameter ratio can improve the performance of the pulse riser.

关键词： numerical simulation fluidized bed entrained flow bed pyrolysis-gasification

First-Principles Study of Structural,Elastic and Electronic Properties of OsSi

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Communications in Theoretical Physics 2009年第10期52卷 701-706页

作者： LI Jin LINGHU Rong-Feng yang ze-Jin CAO yang yang Xiang-Dong Institute of Atomic and Molecular Physics of Sichuan University Chengdu 610065 China College of Material and Chemical Engineering Hainan University Haikou 570228 China Hainan Provincial Key Laboratory of Research on Utilization of Si-Zr-Ti Resources Haikou 570228 China School of Physics and Electronic Science Guizhou Normal University Guiyang 550001 China

First-principles study of structural, elastic, and electronic properties of the B20 structure OsSi has been reported using the plane-wave pseudopotential density functional theory method. The calculated equilibrium lattice and elastic constants are in good agreement with the experimented data and other theoretical results. The dependence of the elastic constants, the aggregate elastic modulus, the deviation from the Cauchy relation, the elastic wave velocities in different directions and the elastic anisotropy on pressure have been obtained and discussed. This could be the first quantitative theoretical prediction of the elastic properties under high pressure of OsSi compound. Moreover, the electronic structure calculations show that OsSi is a degenerate semiconductor with the gap value of 0.68 eV, which is higher than the experimental value of 0.26 eV. The analysis of the PDOS reveals that hybridization between Os d and Sip states indicates a certain covalency of the Os-Si bonds.

关键词： OsSi elastic constants electronic properties high pressure effect

Effect of Guide Vane on Pressure Loss and Heat Transfer Characteristics of Supercritical CO_(2)in U-Shaped Channel

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Journal of Thermal Science 2022年第3期31卷 701-711页

作者： LUO Xiaobo yang ze-Nan ZHANG Jianwu CHEN Wei CHYU Minking School of Aeronautics and Astronautics Sichuan UniversityChengdu610065China Sichuan Gas Turbine Establishment Aero Engine Corporation of ChinaMianyang621703China

U-shaped channel is a common unit structure in heat *** pressure loss and heat transfer characteristics of U-shaped channel have important influence on the comprehensive performance of heat *** this paper,combined with the thermo-physical property of supercritical CO_(2)(SCO_(2)),the influence of the guide vanes on the pressure loss and heat transfer characteristics of SCO_(2)in U-shaped channel was studied *** four different guide vanes included integral guide vane,two-stage symmetric guide vane,two-stage asymmetric guide vane and discrete guide ***_(2)Real Gas Property file(RGP)was imported into ANSYS CFX commercial software for calculation,and the turbulence model was SST k-ω.At the same time,the influence of different inlet Reynolds numbers and heating conditions of channel wall were also studied.

关键词： supercritical CO_(2) horizontal U-shaped channel guide vane pressure loss heat transfer

Transcriptome Analysis During Tetrasporogenesis of Gracilariopsis lemaneiformis and Preliminary Study of the Expressions of Its Meiotic Genes

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Journal of Ocean University of China 2023年第2期22卷 541-554页

作者： ZHU Weifang yang ze XU Di yang Jinxin ZHANG Yingyue PAN Xiaohui The Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China

The transcriptomes of three different parts of the fertile tetrasporophyte of Gracilariopsis lemaneiformis,including tip(T),middle(M),and subjacent(S)parts,with a gradual tetrasporangium maturity were analyzed and compared to identify the genes involved in the process of *** number of differentially expressed genes(DEGs)for the Gple-S versus Gple-T comparison was 10296,and the numbers of DEGs for the Gple-S versus Gple-M and Gple-T versus Gple-M comparisons were 7435 and 1337,*** Ontology and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed,and the results showed the enrichment of 132 KEGG pathways(corrected P<0.05).A total of 58 DEGs related to meiosis were screened and blasted against 18 meiosis-related genes(dmc1,mlh1,mnd1,msh4,msh2,msh6,mre11,pds5,pms1,rad21,rad50,rad51,smc1,smc2,smc4,smc5,smc6,and spo11),including four meiosis-specific *** transcriptome comparison indicated that in the T part,the meiosis,ribosome,and RNA transport-related genes were mostly up-regulated compared with those in the other two *** the M part,the genes related to ribosomes and the endoplasmic reticulum were also up-regulated compared with those in the lower ***,in the S part,the genes associated with photosynthesis were mostly up-regulated,which might be helpful to the recovery from spore formation and release.

关键词： Gracilariopsis lemaneiformis transcriptome tetrasporogenesis meiosis related genes

The Effect of Retardation on the Spontaneous Breaking of Chiral Symmetry in GCM

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Communications in Theoretical Physics 2001年第11期36卷 589-592页

作者： yang Sheng-Dong ZHAI Chen-yang ZHOU Zhi-Ning yang ze-Sen Department of Physics Peking UniversityBeijing 100871China Department of Technical PhysicsPeking UniversityBeijing 100871China Department of PhysicsPeking UniversityBeijing 100871China Department of PhysicsPeking UniversityBeijing 100871China

An effective Hamiltonian including current-current coupling from the global color symmetry model is -R2 *** effects are introduced by the factor ( R/ ) e ,instead of δ (r) in the correlation kernel,from which the retardation gap equation with α-α coupling in the 3po vacuum is obtained,qq condensations of different retardation parameters R with or without the α-α term are *** results show the effects of retardation,and indicate that the typical value of R is about 2 fm-1 at reasonable value of qq *** while taking typical value 1 fm-1 of R,the condensation 1/3 is about 13% larger than that with no retardation *** the α-αterms,the condensation (qq) 1/3 is about 17%o larger than that without it for all values of the parameter *** shows that the retardation effects and the α-α terms are important for further studying in the Iow-energy region.

关键词： effect of retardation,condensation,spontaneous breaking of chiral symmetry

Chemical Properties,Low-Temperature Performance,and Burning Performance of Bio-Jet Fuel

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China Petroleum Processing & Petrochemical Technology 2023年第4期25卷 23-30页

作者： Zhang Zongwei Wang Zihan yang ze Li Junqi Liu Haonan College of Aeronautical Engineering Civil Aviation University of ChinaTianjin300300China Institute for Environment and Development Civil Aviation University of ChinaTianjin300300China Transportation Science and Engineering Civil Aviation University of ChinaTianjin300300China

The effects of composition of bio-jet fuels on freezing point,smoke point,distillation range,and flash point are *** tests using petroleum-based jet fuel with different compositions are conducted in line with standard test *** results show that alkylbenzenes reduce the freezing point,with the amount of reduction becoming greater with increasing alkylbenzene *** a 25%content of the C_8 aromatic xylene,the freezing point is in the range from-55.5℃to-60.1℃.The presence of monocyclic aromatic hydrocarbons leads to an increase in carbon deposition performance,especially when alkyl side chains are *** cycloalkanes,carbon deposition is reduced,although alkyl side chains weaken this beneficial *** hydrocarbons are beneficial in reducing carbon *** ASTM D7566 specifications for the distillation range are based on the average properties of petroleum-based aviation fuel,and if all the parameters governing the distillation range of an alternative jet fuel are at their lower limits,it is necessary to check whether other performance standards are *** components affect the flash point greatly,with 5%of benzene reducing the flash point by 17℃,whereas intermediate components(e.g.,xylene and ethylbenzene)and heavier components have less *** alcohols provide better combustion performance,their content must be strictly limited owing to their effects in reducing the flash point.

关键词： sustainable aviation fuel freezing point smoke point distillation range flash point

Molecular Dynamics Simulation Studies on the Cooling Process of Polyvinyl Chloride

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Chemical Research in Chinese Universities 2006年第1期22卷 80-84页

作者： yang Hua LI ze-sheng yang Yong-biao ZHANG Xiu-bin SUN Chia-chung State Key Laboratory of Theoretical and Computational Chemistry Institute of Theoretical Chemistry Jilin University Changchun 130023 P. R. China

By means of molecular dynamics simulation, the transition of the conformations of polyvinyl chloride during a cooling process from 600 to 300 K was studied. The results show that the amorphous polyvinyl chloride chain experiences the melting state, elastic state and glass state and the conformations can be characterized by the increases of the trans-state of C--C--C--C and the near gauche-state of C--C--C--C1 with the decrease of temperature. It is found that the transition of the conformations is driven mainly by the Coulomb interaction between chain segments.

关键词： Molecular dynamics simulation Polyvinyl chloride Cooling Conformation