检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

毛竹萜类合成酶基因家族序列鉴定与表达分析

植物科学学报 2018年第4期36卷 575-585页

作者：秦政郑永杰张文根张龙黎祖尧杨光耀江西农业大学江西省竹子种质资源与利用重点实验室南昌330045 江西省林业科学院南昌330032 江西特色林木资源培育与利用2011协同创新中心南昌330045

通过生物信息学方法,对毛竹(Phyllostachys edulis(Carrière)***)TPS基因家族的成员进行鉴定,并对其编码蛋白的理化性质、基因结构、进化关系、蛋白结构、启动子元件及表达模式进行了分析。结果表明,毛竹全基因组含有14个TPS候选基因,... 详细信息

通过生物信息学方法,对毛竹(Phyllostachys edulis(Carrière)***)TPS基因家族的成员进行鉴定,并对其编码蛋白的理化性质、基因结构、进化关系、蛋白结构、启动子元件及表达模式进行了分析。结果表明,毛竹全基因组含有14个TPS候选基因,大小为693~2439 bp。编码蛋白等电点为5.08~8.17。系统发育分析结果显示,毛竹含有TPS-a、TPS-b、TPS-e/f、和TPS-g 4个亚家族,成员数目分别为6、5、2、1个。TPS蛋白质二级结构中,α-螺旋和无规则卷曲所占比重较大;毛竹TPS基因家族各成员蛋白三维结构比较相似。基因启动子分析共获得50个调控元件,可分为6大类,其中光响应相关元件数量最多,共包含17个顺式调控元件。基于转录组测序数据构建的基因表达谱热图分析结果表明,Pe TPS在叶、花和笋等7个组织中的表达差异明显,表现出组织特异性,其中Pe TPS9仅在早花期花序中表达,Pe TPS8仅在叶中表达。

关键词：毛竹萜类合成酶进化分析表达模式

在线全文

学校读者我要写书评

暂无评论

燃油系统模糊神经网络故障诊断专家系统研究

内燃机 2006年第3期22卷 25-27,30页

作者：杨昆欧阳光耀海军工程大学动力工程学院湖北武汉430033

专家系统前景广阔,前人在该领域的理论和实践方面已做了大量的工作,但传统的专家系统存在许多缺陷,因此欲使其得到进一步发展就必须引入其它先进技术。为解决传统专家系统知识获取和经验性知识的不确定性等“瓶颈”问题,提出将模糊神经... 详细信息

专家系统前景广阔,前人在该领域的理论和实践方面已做了大量的工作,但传统的专家系统存在许多缺陷,因此欲使其得到进一步发展就必须引入其它先进技术。为解决传统专家系统知识获取和经验性知识的不确定性等“瓶颈”问题,提出将模糊神经网络技术引入到专家系统中,介绍了基于模糊神经网络的专家系统构成的原理、方法、结构和实现技术。将基于模糊多层感知器构成的专家系统应用于柴油机燃油系统故障的分类诊断中,收到了很好的效果。

关键词：神经网络模糊量化专家系统

预测脓毒症患者预后的两种简化SOFA评分方法比较

在线全文

学校读者我要写书评

暂无评论

解放军医学杂志 2020年第7期45卷 742-745页

作者：石齐芳盛鹰王树云蔡金芳杨光耀杨玉英上海市浦东医院/复旦大学附属浦东医院急危重症医学科上海201399

目的比较电子SOFA(eSOFA)评分和简化SOFA(sSOFA)评分对脓毒症患者预后的预测价值。方法回顾性分析2016年8月-2019年8月复旦大学附属浦东医院急危重症医学科收治的245例脓毒症患者的临床资料,以入院后28d患者生存状态为观察终点,使用受... 详细信息

目的比较电子SOFA(eSOFA)评分和简化SOFA(sSOFA)评分对脓毒症患者预后的预测价值。方法回顾性分析2016年8月-2019年8月复旦大学附属浦东医院急危重症医学科收治的245例脓毒症患者的临床资料,以入院后28d患者生存状态为观察终点,使用受试者工作特征曲线下面积(AUC)评价eSOFA评分、sSOFA评分、快速SOFA(qSOFA)评分、SOFA评分预测患者预后的价值。结果eSOFA评分、sSOFA评分、qSOFA评分、SOFA评分预测脓毒症休克患者预后的AUC分别为0.757(95%CI 0.686~0.828)、0.721(95%CI 0.665~0.787)、0.662(95%CI 0.586~0.738)、0.806(95%CI 0.747~0.866)。正态性Z检验结果显示,eSOFA评分优于sSOFA评分(Z=2.317,P=0.021)。结论eSOFA、sSOFA评分对脓毒症预后均有一定预测价值,eSOFA评分优于sSOFA评分。

关键词： SOFA评分脓毒症简化SOFA评分电子健康记录评分系统

在线全文

学校读者我要写书评

暂无评论

不同强度间伐对毛竹根系N、P含量的影响

江西农业大学学报 2020年第2期42卷 358-366页

作者：邱丽君汪子微张扬陈伏生李光敏杨光耀万松泽江西农业大学林学院江西南昌330045

【目的】间伐和林下植被剔除是常见的森林可持续经营措施,但间伐和林下植被剔除对毛竹根系氮(N)、磷(P)养分含量的影响鲜有报道。【方法】选择中亚热带广泛分布的毛竹人工林为研究对象,开展不同强度间伐(25%间伐、50%间伐)和林下植被剔... 详细信息

【目的】间伐和林下植被剔除是常见的森林可持续经营措施,但间伐和林下植被剔除对毛竹根系氮(N)、磷(P)养分含量的影响鲜有报道。【方法】选择中亚热带广泛分布的毛竹人工林为研究对象,开展不同强度间伐(25%间伐、50%间伐)和林下植被剔除对毛竹鞭根和篼根N、P养分含量及根际土壤理化特性的影响研究。【结果】林下植被剔除对毛竹根系养分含量无显著影响。25%间伐处理增加根际土壤P含量,加强篼根根际效应,促进根系对根际土壤N、P的吸收,显著增加篼根全N含量和鞭根全P含量(P<0.01),有利于促进毛竹根系的生长,提高毛竹生产力。50%间伐显著增加篼根N含量,但对篼根P含量、鞭根N、P含量均无显著影响。【结论】从长远来看,随着植物对土壤P需求的增加,土壤P输入的持续减少可能加剧毛竹人工林土壤P的限制作用,不利于毛竹林生产力的提高。此外,本研究结果表明,毛竹篼根和鞭根养分含量对间伐处理的响应差异明显,可能与毛竹篼根和鞭根的功能差异性有关,然而目前缺乏相关的研究,在今后的研究中有待补充加强。

关键词：间伐根系养分篼根鞭根毛竹

[Solid-phase extraction coupled with high performance liquid chromatography-triple quadrupole mass spectrometry for simultaneous determination of seven coumarins in water samples from drinking water treatment plants]

在线全文

学校读者我要写书评

暂无评论

Se pu = Chinese journal of chromatography 2025年第1期43卷 78-86页

作者： Wen-Mei Jiao Jing-Ming yang Ce Xu Fu-Kang Gao Lu-yao Shen Yu-Bo Yuan Zhi-Fen Guo guang Huang School of Life Sciences and Chemical Engineering Jiangsu Second Normal University Nanjing 211200 China. School of Stomatology Nanjing Medical University Nanjing 210029 China. School of Public Health Nanjing Medical University Nanjing 211166 China. Key Laboratory of Modern Toxicology Ministry of Education School of Public Health Nanjing Medical University Nanjing 211166 China.

Chlorinated coumarins, which are as cytotoxic as highly toxic halobenzoquinones toward CHO-K1 cells, have recently been identified as disinfection byproducts in drinking water disinfection processes. Therefore, detecting coumarins in water samples collected at various stages from drinking water treatment plants helps assess the formation of chlorinated coumarins in drinking water. Hence, a simple, rapid, accurate, and sensitive method for quantifying coumarins in water samples is required. In this study, a method was developed based on solid-phase extraction coupled with high performance liquid chromatography-triple-quadrupole mass spectrometry for analyzing seven coumarins in water samples from drinking water treatment plants, including 6,7-dihydroxycoumarin, 7-hydroxycoumarin, 6-hydroxy-4-methylcoumarin, 8-chloro-7-hydroxycoumarin, coumarin, 7-chloro-6-hydroxy-4-methylcoumarin, and 3,8-dichloro-7-hydroxycoumarin. Sample pretreatment involved solid-phase extraction using HLB columns, followed by elution with water and methanol, each containing 0.25% formic acid. The extracted solution was separated via gradient elution using a Phenomenex Luna C18 column (100 mm×2.0 mm, 3 mm) with 0.1% formic acid aqueous solution and methanol as the mobile phases, with analytes detected by triple-quadrupole mass spectrometry equipped with electrospray ionization source in multiple reaction monitoring mode. The matrix effect, precision, and accuracy of the developed method were investigated using raw and treated water as matrices. Matrix effects of 0.84-1.12 were recorded for the detection of 6,7-dihydroxycoumarin, 7-hydroxycoumarin, 6-hydroxy-4-methylcoumarin, and coumarin in raw water, while values of 0.67-0.70 were recorded for 8-chloro-7-hydroxycoumarin, 7-chloro-6-hydroxy-4-methylcoumarin, and 3,8-dichloro-7-hydroxycoumarin in finished water. The three chlorinated coumarins exhibited matrix effects above 0.80 after the finished water matrix had been diluted four times. These re

关键词： coumarins disinfection byproducts drinking water high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) solid-phase extraction (SPE)

Anomalous quality factor evolution of induced transparency in a virtualized coupled-oscillator system

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 148-155页

作者： Yunhan yang Han Jia Tuo Liu Yuzhen yang Jun yang Key Laboratory of Noise and Vibration Research Institute of Acoustics Chinese Academy of Sciences University of Chinese Academy of Sciences State Key Laboratory of Acoustics Institute of Acoustics Chinese Academy of Sciences

The phenomenon of induced transparency, characterized by a high quality(Q) factor, holds significant importance in the fields of high-precision sensing, energy storage and wave manipulation. Conventional transparency systems are often constrained by the limited experimentally accessible parameter space and the lack of independent control over intrinsic parameters, thereby hindering advanced research on high-Q effects. Here we propose a virtualized coupled-oscillator system that enables reconfigurable induced transparency in the reflection spectrum. By virtually incorporating a pair of coupled oscillators into an individual acoustic meta-atom, we achieve the manipulation of induced transparency in a decoupled, wide-ranging, and elaborate manner. Exploiting the diverse evolutions across the intrinsic parameter space, we show experimentally a counter-intuitive increase in the Q-factor of the transparency window, which is attributed to the enhanced dissipation of the bright ***, a constant group delay at the center frequency is observed accompanying this evolution. Our work demonstrates an intriguing platform as well as a novel strategy to engineer high-Q induced transparency phenomenon, and paves the way towards applications in programmable acoustic sensors, filters and slow-wave devices.

关键词： induced transparency acoustic meta-atom high quality-factor scattering programmable intrinsic parameter

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

厚壁毛竹展叶期竹秆内源激素特征

江西农业大学学报 2012年第1期34卷 54-58页

作者：方楷杨光耀杨清培施建敏于芬江西农业大学江西省竹子种质资源与利用重点实验室江西南昌330045

应用高效液相色谱(HPLC)技术测定了厚壁毛竹展叶期竹秆不同高度位置上内源激素的组成及含量。结果表明:展叶期竹秆中内源激素的平均含量(μg/g)从高到低依次为:GA3(77.30±5.81)、IBA(4.36±0.85)、IAA(3.21±0.73)、ZT(2.63±0.26)、2-... 详细信息

应用高效液相色谱(HPLC)技术测定了厚壁毛竹展叶期竹秆不同高度位置上内源激素的组成及含量。结果表明:展叶期竹秆中内源激素的平均含量(μg/g)从高到低依次为:GA3(77.30±5.81)、IBA(4.36±0.85)、IAA(3.21±0.73)、ZT(2.63±0.26)、2-ip(2.22±0.35)、6-BA(2.01±0.28)、ABA(1.40±0.10)。2-ip、GA3、ABA、6-BA和ZT的含量均随竹秆位置降低而不断下降,但2-ip、GA3和ABA的含量在中部下降不明显,而梢部和基部位置均呈明显下降趋势;6-BA含量随竹秆位置降低呈近线性减小;ZT含量在梢部下降较快,而中部和基部下降不明显。不同位置按IAA、IBA含量的高低均排列为:中部、梢部、基部。内源激素含量间有明显的相关性,GA3与2-ip的相关性达到极显著水平(R=0.854,P<0.01),ZT与GA3、ABA间、2-ip与ABA间的相关性均达到显著性水平(P<0.05)。

关键词：厚壁毛竹内源激素展叶期竹秆

Unraveling brain aging through the lens of oral microbiota

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si Wang Weiqi Zhang Jing Qu guang-Hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

在线全文

学校读者我要写书评

暂无评论

铁型覆砂磨球铸造工艺设计及模拟分析

铸造 2014年第9期63卷 891-893,899页

作者：杨光耀朱协彬王仲珏周道宏房文亮安徽工程大学机械与汽车工程学院安徽芜湖241000 安徽省宁国新宁实业有限公司安徽宁国242300

介绍了铁型覆砂磨球铸造工艺流程及质量要求。在铁型覆砂磨球铸造工艺设计的基础上,运用铸造模拟软件MAGMAsoft进行工艺仿真,分析工艺模拟的结果,提出工艺优化方案。在实际生产中验证工艺的合格性,从而达到缩短研发周期、提高铸件质量... 详细信息

介绍了铁型覆砂磨球铸造工艺流程及质量要求。在铁型覆砂磨球铸造工艺设计的基础上,运用铸造模拟软件MAGMAsoft进行工艺仿真,分析工艺模拟的结果,提出工艺优化方案。在实际生产中验证工艺的合格性,从而达到缩短研发周期、提高铸件质量和降低生产成本的目的。

关键词：铁型覆砂磨球铸造模拟分析工艺优化