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Super-Resolution Fluorescence Microscopy for Cilia Investigation and Ciliopathy Diagnosis（特邀）

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激光与光电子学进展 2024年第6期 270-281页

作者： Liu Zhen Wu yang The Hong Kong University of Science and Technology Division of Life Science

The last two decades have witnessed the invention and development of super-resolution microscopy(SRM) that breaks the diffraction limit of light and pushes the fluorescence microscopy resolution to several nanometers. While SRM is widely used in biological studies, such as resolving subdiffraction structures, molecular mapping, tracking single molecules, probing protein-protein interactions, and observing organelle dynamics, its direct application in translational medicine, such as disease diagnosis, is still preliminary. Despite their small size, cilia play a crucial role as organelles in cell signaling and motility, with defects in cilia leading to ciliopathy. Similar to other miniature organelles and macromolecular complexes, cilia are ideal for super-resolution imaging. In this review, we will 1) introduce cilia and ciliopathy, 2) show how SRM extends our knowledge of cilia, and 3) focus on how SRM improves the diagnosis of motile ciliopathies.

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POX1 regulates the formation of viable but non-culturable brewer’s yeast induced by iso-alpha acid from hops

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Food Science and Human Wellness 2024年

作者： Zengyan Wang yang Xiao Xuchen Li Zhenqing Li Yan Wang Wei He Jiayang Wang Li Liu Ting Ding Pengdong Sun Jingyuan Li yang Deng Shandong Technology Innovation Center of Special Food 4. Qingdao Special Food Research Institute Key Laboratory of Special Food Processing (Co-construction by Ministry and Province)， Ministry of Agriculture Rural Affairs， Qingdao Agricultural University School of Food Engineering， Qingdao Institute of Technology College of Food Science and Engineering， Qingdao Agricultural University Shandong Huifa Foodstuff Co.， Ltd.

In this study， we utilized gene knockout and overexpression techniques to generate brewer’s yeast strains with either a deletion or overexpression of the POX1 gene. The strains studied included the parental strain， the POX1 deletion strain， and the POX1 overexpression strain. These strains were exposed to iso-alpha acid from hops at a concentration of 300 mg/L， leading to the induction of a viable but non-culturable (VBNC) state. Our results indicated that the silencing of the POX1 gene rendered brewer’s yeast cells unable to withstand the high concentration of iso-alpha acid stress， ultimately leading to cell death. Conversely， the overexpression of POX1 accelerated the transition of yeast cells into the VBNC state compared to the parental strain. Furthermore， we evaluated the levels of reactive oxygen species (ROS)， catalase (CAT) activity， superoxide dismutase (SOD) activity， glutathione reductase (GR) activity， and the mRNA expression of genes that regulate these enzymes (SYM1， CTA1， SOD1， and GLR1) in brewer’s yeast cells at three distinct stages: normal， short-term stress， and VBNC states. Based on these findings， it can be inferred that the formation of the VBNC state in brewer’s yeast is associated with the response to oxidative stress.

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亚硝基苯参与的电化学串联环化反应构建喹啉/吡咯

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有机化学 2024年第3期44卷 1021-1030页

作者：杨帆方婷杨桂春高梦湖北大学化学化工学院、有机功能分子合成与应用教育部重点实验室武汉430062 武汉大学化学与分子科学学院武汉430072

报道了一种多取代喹啉/吡咯杂环化合物的电化学合成新方法.反应以亚硝基苯和贫电子内炔为起始物,在无需额外金属催化剂、室温条件下,通过阴极单电子还原的策略,选择性地还原亚硝基苯产生亚硝基自由基,随后与炔烃进行串联环化反应,绿色... 详细信息

报道了一种多取代喹啉/吡咯杂环化合物的电化学合成新方法.反应以亚硝基苯和贫电子内炔为起始物,在无需额外金属催化剂、室温条件下,通过阴极单电子还原的策略,选择性地还原亚硝基苯产生亚硝基自由基,随后与炔烃进行串联环化反应,绿色、高效地合成了一系列具有高附加值的多取代喹啉/吡咯杂环化合物.

关键词：电化学合成串联环化亚硝基苯

Permian tectonic evolution and continental accretion in the eastern Central Asian Orogenic Belt:A perspective from the intrusive rocks

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Geoscience Frontiers 2024年第2期15卷 433-451页

作者： Anzong Fu Hongyan Geng Changzhou Deng Chenglu Li Jishuang Ding Bizheng yang Wenpeng yang Heilongjiang Institute of Natural Resources Survey Harbin 150036China State Key Laboratory of Ore Deposit Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550002China Science Unit Lingnan UniversityHong KongChina College of Earth Sciences Jilin UniversityChangchun 130061China

The tectonic evolution and history of continental accretion of the eastern Central Asian Orogenic Belt(CAOB)are not yet fully *** this study,we investigate Permian intrusive rocks from the Jiamusi Block of the eastern CAOB to constrain the tectonic evolution and continental accretion of this region during the late-stage evolution of the Paleo-Asian *** new data show that Early Permian gabbro-diorites were derived from the partial melting of depleted mantle metasomatized by oceanic-slab-released *** Permian adakitic granites have low Na2O and MgO and high K2O contents,indicating a thickened-lower-crust *** Permian S-type granites were derived from the partial melting of continental crust.A compilation of the available geochronological data for Permian intrusive rocks(including adakitic and A-,S-,and I-type granites and mafic rocks)from the eastern CAOB reveals that the A-type granites formed mainly during the Early–Middle Permian,S-type and adakitic granites mostly during the Middle–Late Permian,and I-type granites and mantle-derived mafic rocks throughout the *** A-type granites,which are proposed to have been sourced from thinned continental crust,indicate an extensional setting in the eastern CAOB during the Early *** Middle–Late Permian adakitic granites imply a thickened continental crust,which indicates a compressional ***,the eastern CAOB underwent a transition from extension to compression during the Middle Permian,which was probably triggered by the late-stage subduction of Paleo-Asian oceanic *** the petrogenesis of the intrusive rocks and inferred regional tectonic evolution of the eastern CAOB,we propose that vertical underplating of mantle-and oceanic-slabderived magmas contributed the materials for continental crust accretion.

关键词： Permian Intrusive rocks Eastern Central Asian Orogenic Belt Paleo-Asian Ocean Tectonic evolution

Modeling the performance of perovskite solar cells with inserting porous insulating alumina nanoplates

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Chinese Physics B 2024年第3期33卷 668-671页

作者：潘赵耀杨金彭沈小双 College of Physical Science and Technology Yangzhou UniversityYangzhou 225009China

Peng et al.[Science 379683(2023)]reported an effective method to improve the performance of perovskite solar cells by using thicker porous insulator contact(PIC)-alumina *** method overcomes the trade-off between the open-circuit voltage and the fill factor through two mechanisms:reduced surface recombination velocity and increased bulk recombination lifetime due to better perovskite *** arguments of drift-diffusion simulations,we find that an increase in mobility and carrier recombination lifetime in bulk are the key factors for minimizing the resistance-effect from thicker PICs and achieving a maximum power conversion efficiency(PCE)at approximately 25%reduced contact ***,the partially replacement of perovskite films with thicker PICs would result in a reduction in short-current density,but the relative low refractive index of the PICs imbedded into the high refractive index perovskite creates light trapping structures that compensate for this loss.

关键词： perovskite solar cells nanostructure crystalline mobility

Rhizosphere microbial markers (micro-markers): A new physical examination indicator for traditional Chinese medicines

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Chinese Herbal Medicines 2024年第2期16卷 180-189页

作者： Kailin yang Yaping Zheng Kangmeng Sun Xinyan Wu Zheng Zhang Chunnian He Peigen Xiao Institute of Medicinal Plant Development Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of EducationBeijing 100193China

Rhizosphere microorganisms,as one of the most important components of the soil microbiota and plant holobiont,play a key role in the medicinal plant-soil ecosystem,which are closely related to the growth,adaptability,nutrient absorption,stress tolerance and pathogen resistance of host *** recent years,with the wide application of molecular biology and omics technologies,the outcomes of rhizosphere microorganisms on the health,biomass production and secondary metabolite biosynthesis of medicinal plants have received extensive ***,whether or to what extent rhizosphere microorganisms can contribute to the construction of the quality evaluation system of Chinese medicinal materials is still *** on the significant role of rhizosphere microbes in the survival and quality formation of medicinal plants,this paper proposed a new concept of rhizosphere microbial markers(micro-markers),expounded the relevant research methods and ideas of applying the new concept,highlighted the importance of micro-markers in the quality evaluation and control system of traditional Chinese medicines(TCMs),and introduced the potential value in soil environmental assessment,plant pest control and quality assessment of *** provides reference for developing ecological planting of TCMs and ensuring the production of high quality TCMs by regulating rhizosphere microbial communities.

关键词： physical examination indicator quality evaluation quality formation of medicinal plants rhizosphere microorganisms rhizosphere microbial markers

基于模型预测的膝关节置换手术机器人柔顺控制

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工程科学学报 2024年第9期46卷 1638-1646页

作者：胡飘张丽杨闳竣杨妍中国矿业大学(北京)人工智能学院北京100083 中国科学院自动化研究所多模态人工智能系统全国重点实验室北京100190 北京科技大学智能科学与技术学院北京100083

针对半主动型膝关节置换手术机器人的柔顺跟随医生意图运动和手术安全范围约束问题,提出了一种模型预测导纳控制算法.首先,为了提高算法运行效率,使用斯特林插值方法对机械臂动力学模型进行线性化,并作为预测模型,该方法计算简单且求解... 详细信息

针对半主动型膝关节置换手术机器人的柔顺跟随医生意图运动和手术安全范围约束问题,提出了一种模型预测导纳控制算法.首先,为了提高算法运行效率,使用斯特林插值方法对机械臂动力学模型进行线性化,并作为预测模型,该方法计算简单且求解精度高;其次,基于导纳模型力柔顺控制机理识别操作医生的力意图生成机械臂期望运动轨迹;然后,设计虚拟状态,提高模型预测控制算法的显式处理约束能力,利用模型预测控制的滚动优化和反馈校正特性提高控制鲁棒性;最后,结合成机械臂模型预测导纳控制器.设计三环PID(Proportional integral derivative)控制对照试验,验证了模型预测控制算法的轨迹跟踪性能更好,可以更好的实现期望的导纳动态,从而得到更优的柔顺效果.在此基础上,进一步验证模型预测导纳控制器在具有强耦合性、复杂系统参数结构特性的机械臂上的主动约束效果.结果表明模型预测导纳控制算法能实现比传统三环PID控制更好的柔顺性,且具备满足膝关节置换手术需求的安全性.本文有望促进半主动手术机器人的实际应用.

关键词：手术机器人人机交互模型预测导纳控制安全约束

Surface-to-bulk engineering with high-valence W6+ enabling stabilized single-crystal LiNi0.9Co0.05Mn0.05O2 cathode

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Journal of Energy Chemistry 2024年第11期 67-76页

作者： Jun-Ke Liu Xue-Rui yang Chuan-Wei Wang Zu-Wei Yin Yi-yang Hu Li Deng Zhen Wang Yao Zhou Jun-Tao Li College of Energy Xiamen University School of Physics and Materials Science Nanchang University College of Chemistry and Chemical Engineering Xiamen University

Single-crystal Nickel-rich layered oxides has been recognized as one of the promising cathodes for nextgeneration lithium batteries on account of their high capacity,while its practical application was hindered by structural instability and slow Li+transfer ***,a surface-to-bulk engineered single-crystal LiNi0.9Co0.05Mn0.05O2（Ni90） cathode,which features W-doped bulk and Li2WO4surface layer,was successfully achieved by a one-step high-valence W6+*** as-obtained W-modified Ni90 delivers excellent cycling stability（89.8% capacity retention after 300 cycles at 0.5 C）and rate *** enhanced electrochemical performance was ascribed to the doped-W induced stabilized lattice oxygen,reduced Li+/Ni2+mixing and inhibited H2-H3 phase transition in the bulk,and Li2WO4layer generated stabilized cathode/electrolyte *** addition,the thinner LiF-rich cathode electrolyte interphase（CEI） on surface and smaller grain size for W-modified Ni90 benefit to its Li+diffusion *** effect of high-valence W6+on single-crystal Ni-rich cathode was firstly revealed in detail,which deepens the understanding of electrochemical behavior of Ni-rich cathode with high-valence cations modification,and provides clues for design of high-performance layered cathodes.

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Structural engineering of Fe single-atom oxygen reduction catalyst with high site density and improved mass transfer

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Journal of Energy Chemistry 2024年第11期 634-644页

作者： Jiawen Wu Yuanzhi Zhu An Cai Xiaobin Fan Wenchao Peng yang Li School of Chemical Engineering and Technology Tianjin University Faculty of Chemical Engineering Yunnan Provincial Key Laboratory of Energy Saving in Phosphorus Chemical Engineering and New Phosphorus MaterialsKunming University of Science and Technology Institute of Shaoxing Tianjin University

Fe-N-C catalysts are widely considered as promising non-precious-metal candidates for electrocatalytic oxygen reduction reaction（ORR）,Yet despite their high catalytic activity through rational modulation,challenges remain in their low site density and unsatisfactory mass transfer ***,we present a structural engineering approach employing a soft-template coating strategy to fabricate a hollow and hierarchically porous N-doped carbon framework anchored with atomically dispersed Fe sites（FeNCh） as an efficient ORR *** combination of hierarchical porosity and high exterior surface area is proven crucial for exposing more active sites,which gives rise to a remarkable ORR performance with a half-wave potential of 0.902 V in 0.1 m KOH and 0.814 V in 0.1 m HClO4,significantly outperforming its counterpart with solid structure and dominance of micropores（FeNC-s）.The mass transfer property is revealed by in-situ electrochemical impedance spectroscopy（EIS） *** distribution of relaxation time（DRT） analysis is further introduced to deconvolve the kinetic and mass transport processes,which demonstrates an alleviated mass transport resistance for FeNC-h,validating the effectiveness of structural *** work not only provides an effective structural engineering approach but also contributes to the comprehensive mass transfer evaluation on advanced electrocatalyst for energy conversion applications.

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