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Arabidopsis EXO70A1 recruits Patellin3 to the cell membrane independent of its role as an exocyst subunit

Journal of Integrative Plant Biology 2017年第12期59卷 851-865页

作者： Chengyun Wu Lu Tan Max van Hooren Xiaoyun Tan Feng Liu yan Li yanxue zhao Bingxuan Li Qingchen Rui Teun Munnik Yiqun Bao College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China Department of Plant Cell Biology Swammerdam Institute for Life Sciences(SILS)University of AmsterdamScience park 9o41o98 XHAmsterdamThe Netherlands

The exocyst is a well-known complex which tethers vesicles at the cell membrane before fusion. Whether an individual subunit can execute a unique function is largely unknown. Using yeast-two-hybrid （Y2H） analysis, we found that EXO7oA1 interacted with the GOLD domain of Patellin3 （PATL3）. The direct EXO7OA1-PATL3 interaction was supported by in vitro and in vivo experiments. In Arabidopsis, PATL3-GFP colocalized with EXO7oA1 predominantly at the cell membrane, and PATL3 localization was insensitive to BFA and TryA23. Remarkably, in the exo7oa1 mutant, PATL3 proteins accumulated as punctate structures within the cytosol, which did not colocalize with several endomembrane compartment markers, and was insensitive to BFA. Furthermore, PATL3 localization was not changed in the exo7oe2, PRsec6 or exo84b mutants. These data suggested that EXO7oA1, but not other exocyst subunits, was responsible for PATL3 localization, which is independent of its role in secretory/recycling vesicletethering/fusion. Both EXO7oA1 and PATL3 were shown to bind PI4P and PI（4,5）P2 in vitro. Evidence was obtained that the other four members of the PATL family bound to EXO7oA1 as well, and shared a similar localization pattern as PATL3. These findings offered new insights into exocyst subunitspecific function, and provided data and tools for further characterization of PATL family proteins.

关键词： Figure Arabidopsis EXO70A1 recruits Patellin3 to the cell membrane independent of its role as an exocyst subunit

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Study of organic solar cells with stacked bulk heterojunction structure

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Optoelectronics Letters 2008年第4期4卷 257-259页

作者： ZHANG Xin-fang XU Zheng zhao Su-ling ZHANG Fu-jun LI yan WU Chun-yu CHEN Yue-ning Institute of Optoelectronics Technology Key Laboratory for Luminescence and Optical Information of Ministry of Education Beijing Jiaotong University Beijing 100044 China Department of Physics Liaoning University Shenyang 110036 China

Organic solar cells with stacked bulk heterojutaction（BHJ） are investigated based on conjugated polymer. By using the solution spin-coating method, Poly[2-methoxy, 5-（2＇ -ethyl-hexyloxy） -1,4-phenylene vinylene] （MEH-PPV） and ZnO nanoparticles （50 nm） are mixed as the optical sense layer. Ag is used as inter-layer to connect the upper BHJ cell and the lower cell. The structures are ITO/PEDOT：PSS/MEH-PPV lAg / MEH-PPV：ZnO/Al. The open circuit voltage （Voc） of a stacked cell is about 3.7 times of that of an individual organic solar cell （ITO/PEDOT：PSS/MEH-PPV/Al）. The short circuit current （Jsc） of a stacked cell is increased by about 1.6 times of that of individual one.

关键词：有机太阳能电池异质结构共轭聚合体太阳能利用

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Identification of biomarkers for hepatocellular carcinoma by semiquantitative immunocytochemistry

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World Journal of Gastroenterology 2014年第19期20卷 5826-5838页

作者： Hong Mu Kai-Xuan Lin Hong zhao Shu Xing Cong Li Fang Liu Hai-Zhen Lu Ze Zhang Yu-Lin Sun Xi-Yun yan Jian-Qiang Cai Xiao-Hang zhao State Key Laboratory of Molecular Oncology Cancer Institute HospitalChinese Academy of Medical Sciences and Peking Union Medical College Center of Basic Medical Sciences Navy General Hospital of Chinese PLA Department of Abdominal Surgery Cancer Institute HospitalChinese Academy of Medical Science and Peking Union Medical College Key Laboratory of Protein and Peptide Pharmaceutical National Laboratory of BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences Department of Pathology Cancer Institute HospitalChinese Academy of Medical Sciences and Peking Union Medical College

AIM: To investigate the expression of key biomarkers in hepatoma cell lines, tumor cells from patients’ blood samples, and tumor tissues.

关键词： Hepatocellular carcinoma Biomarker Immunocytochemistry Semiquantitative analysis Three-dimensional reconstruction

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Cosmogenic^(10)Be dating of the oldest moraine in the Hengduan Mountains

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Science China Earth Sciences 2023年第10期66卷 2211-2223页

作者： Zhigang ZHANG Jiahong ZHENG Haitao CUI Zhijun zhao Xinggong KONG Chaogang ZHENG Hong ZHANG Fei GUO Zhiyang CHANG Xue YUAN Mingyang yan Jie ZHANG Jian WANG School of Geography Nanjing Normal UniversityNanjing210023China Key Laboratory of Virtual Geographic Environment(Nanjing Normal University) Ministry of EducationNanjing210023China Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application Nanjing210023China Tourism and Social Administration College NanJing XiaoZhuang UniversityNangjing211171China

The Hengduan Mountains is in the transitional zone between the Qinghai-Tibet Plateau(QTP)and the YunnanGuizhou Plateau in China,and a key area for elucidating the Quaternary environmental changes in *** paleo-Daocheng ice cap was located on the Shaluli Hilly Plateau in the northeastern Hengduan Mountains,the oldest moraines in the Hengduan Mountains region were found in the ice cap *** glacial landforms provide key evidence to study the timing when this area entered the cryosphere with the uplift of the ***,it is difficult to collect suitable glacial boulders from these moraines for traditional terrestrial in-situ cosmogenic nuclide(TCN)exposure dating because of long-term severe moraine ***,we collected clast samples from the moraine surface and depth profile to constrain the age of the oldest moraine in Kuzhaori(moraine E)using TCN^(10)Be dating *** minimum^(10)Be ages of five clast samples from the moraine surface range from 187.4±1.5 to 576.8±4.3 ka,implying that the moraine has been seriously degraded since *** on the TCN^(10)Be concentrations of the samples from a depth profile and simulations,the exposure-erosion-inheritance history of the profile was *** fitting to the profile^(10)Be concentrations using the chi-square test,the simulations yielded a reliable age of 626.0±52.5 ka for the ***,the oldest moraine(moraine E)in Kuzhaori was most likely formed at about 0.63Ma ago,corresponding to the marine isotope stage(MIS)*** glaciation represents the maximum Quaternary glaciation after the QTP was elevated into the cryosphere by the Kunlun-Yellow River Tectonic Movement.

关键词： Hengduan Mountains Paleo-Daocheng ice cap Terrestrial in-situ cosmogenic nuclide^(10)Be Depth profile Exposure dating

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Overcoming genotypic dependency and bypassing immature embryos in wheat transformation by using morphogenic regulators

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Science China(Life Sciences) 2024年第7期67卷 1535-1538页

作者： Ziru Zhou Yawen yang Guo Ai Miaomiao zhao Baozhu Han Chunjie zhao Yiqian Chen Yuwei Zhang Hong Pan Caixia Lan Chao He Qiang Li Jieting Xu Wenhao yan National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China WIMI Biotechnology Co. Ltd.Changzhou 213025China

Dear Editor,Agrobacterium-mediated transformation technology is of vital importance for functional genomics studies and precision breeding in crops due to low cost and clear genetic manifestation.A morphogenic gene pa... 详细信息

关键词： breeding crops transformation

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Role of FK506-binding protein in Ca^(2+) spark regulation

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Science Bulletin 2017年第19期62卷 1295-1303页

作者： yan-Ting zhao Yun-Bo Guo Xue-Xin Fan Hua-Qian yang Peng Zhou Zheng Chen Qi Yuan Haihong Ye Guang-Ju Ji Shi-Qiang Wang State Key Laboratory of Membrane Biology College of Life SciencesPeking University National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of Sciences School of Basic Medical Sciences Beijing Institute for Brain Disorders Center of SchizophreniaCapital Medical University

The elementary Ca^2＋ release events, Ca2＋ sparks, has been found for a quarter of century. However, the molecular regulation of the spark generator, the ryanodine receptor （RyR） on the sarcoplasmic reticulum, remains obscure. Although each subunit of the RyR homotetramer has a site for FKS06-binding protein （FKBP）, the role of FKBPs in modifying RyR Ca^2＋ sparks has been debated for long. One of the reasons behind the controversy is that most previous studies detect spontaneous sparks, where the mixture with out-of-focus events and local wavelets prevents an accurate characterization of Ca^2＋ sparks. In the pre- sent study, we detected Ca^2＋ sparks triggered by single L-type Ca^2＋ channels （LCCs） under loose-seal patch clamp conditions in FKS06-treated or FKBPI2.6 knockout cardiomyocytes. We found that FKBP dissociation both by FKS06 and by rapamycin decreased the Ca^2＋ spark amplitude in ventricular cardiomyocytes. This change was neither due to decreased releasable Ca^2＋ in the sarcoplasmic reticulum, nor explained by changed RyR sensitivity. Actually FKS06 increased the LCC-RyR coupling probability and curtailed the latency for an LCC to trigger a RyR Ca^2＋ spark. FKBP12.6 knockout had similar effects as FKS06/rapamycin treatment, indicating that the decreased spark amplitude was attributable to the dissociation of FKBP12.6 rather than FKBP12. We also explained how decreased amplitude of spontaneous sparks after FKBP dissociation sometimes appears to be increased or unchanged due to inappropriate data processing. Our results provided firm evidence that without the inter-RyR coordination by functional FKBP12.6, the RyR recruitment during a Ca^2＋ spark would be compromised despite the sensitization of individual RyRs.

关键词： Ca^2+ sparkFKSO6-binding protein Ryanodine receptorlntracellular calcium Excitation-contraction coupling

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Multi-locus variable-number tandem repeat analysis of Chinese Brucella strains isolated from 1953 to 2013

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Infectious Diseases of Poverty 2017年第1期6卷 778-785页

作者： Guo-Zhong Tian Bu-Yun Cui Dong-Ri Piao Hong-yan zhao Lan-Yu Li Xi Liu Pei Xiao Zhong-Zhi zhao Li-Qing Xu Hai Jiang Zhen-Jun Li State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Prevention and ControlBeijing 102206China National Institute of Occupational Health and Poison Control Chinese Center for Disease Prevention and ControlBeijing 100050China Department of Brucellosis Prevention and Control Qinghai Institute for Endemic Disease Prevention and ControlXining 811602China

Background:Brucellosis was a common human and livestock disease caused by Brucella strains,the category B priority pathogens by the US Center for Disease Control(CDC).Identified as a priority disease in human and livestock populations,the increasing incidence in recent years in China needs urgent control measures for this disease but the molecular background important for monitoring the epidemiology of Brucella strains at the national level is still ***:A total of 600 Brucella isolates collected during 60 years(from 1953 to 2013)in China were genotyped by multiple locus variable-number tandem repeat analysis(MLVA)and the variation degree of MLVA11 loci was calculated by the Hunter Gaston Diversity Index(HGDI)*** charts and map were processed by Excel 2013,and cluster analysis and epidemiological distribution was performed using BioNumerics(version 5.1).Results:The 600 representative Brucella isolates fell into 104 genotypes with 58 singleton genotypes by the MLVA11 assay,including *** biovars 2 and 3(five main genotypes),*** biovars 1 and 3(two main genotypes),*** biovars 1 and 3(three main genotypes),and ***(two main genotypes)*** most *** biovar 1 and biovar 3 were respectively found in northern provinces and southern provinces,*** and *** strains were dominant in *** Brucellosis was only found in animals without any human cases *** Brucellosis epidemic peaks emerged during the 60 years between 1953 and 2013:1955-1959,1962-1969,1971-1975,1977-1983,1985-1989,1992-1997,2000-2008 and 2010-2013 in ***:Brucellosis has its unique molecular epidemiological patterns with specific spatial and temporal distribution according to *** registration:IDOP-D-16-00101.

关键词： Brucella MLVA Molecular epidemiology

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卡车动载实测与分析

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湖南大学学报（自然科学版） 2014年第11期41卷 129-132页

作者：赵延庆白龙刘立安倪远宝大连理工大学交通运输学院辽宁大连116024 辽宁省交通规划设计院辽宁沈阳110166

通过在卡车各轴上安装了变形传感器,标定后得到变形传感器和轮胎接地压力之间的转化关系,进行了卡车动载实测,分析了路面平整度、车速及载重对卡车动载的影响.结果表明,当路面行驶质量指数为优、中、差时,平均动载系数分别为5%、15%和20... 详细信息

通过在卡车各轴上安装了变形传感器,标定后得到变形传感器和轮胎接地压力之间的转化关系,进行了卡车动载实测,分析了路面平整度、车速及载重对卡车动载的影响.结果表明,当路面行驶质量指数为优、中、差时,平均动载系数分别为5%、15%和20%左右,最大动载系数可达25%;最大动载相对于静载平均增加20%、55%和80%左右,最大动载变化率可达90%以上.动载系数、最大动载及其变化率均随车速的增加而增加,当行驶质量指数较差时,增加幅度更为明显.研究成果为在路面结构分析和设计中考虑动载影响提供了基础数据.

关键词：道路工程卡车路面平整度动载系数标定

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First-line penpulimab(an anti-PD1 antibody)and anlotinib(an angiogenesis inhibitor)with nab-paclitaxel/gemcitabine(PAAG)in metastatic pancreatic cancer:a prospective,multicentre,biomolecular exploratory,phase II trial

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Signal Transduction and Targeted Therapy 2024年第7期9卷 2960-2969页

作者： Huizi Sha Fan Tong Jiayao ni Yi Sun Yahui Zhu Liang Qi Xiaoqin Li Wei Li yan yang Qing Gu Xing Zhang Xiaoxuan Wang Chan Zhu Dongsheng Chen Baorui Liu Juan Du The Comprehensive Cancer Center Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing University&Clinical Cancer Institute of Nanjing UniversityNanjingChina The Comprehensive Cancer Center Nanjing Drum Tower HospitalClinical College of Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjingChina Department of Oncology The Affiliated Hospital of Jiangsu UniversityZhenjiangChina Department of Oncology The First Affiliated Hospital of Soochow UniversitySuzhouChina Department of Oncology The Affiliated Jiangning Hospital of Nanjing Medical UniversityNanjingChina National Institute of Healthcare Data Science at Nanjing University NanjingChina State Key Laboratory of Neurology and Oncology Drug Development Jiangsu Simcere Diagnostics Co.Ltd.Nanjing Simcere Medical Laboratory Science Co.Ltd.NanjingChina

Metastatic pancreatic cancer(mPC)has a dismal ***,we conducted a prospective,multicentre,single-arm,phase II trial evaluating the efficacy and safety of penpulimab and anlotinib in combination with nab-paclitaxel/gemcitabine(PAAG)in patients with first-line mPC(NCT05493995).The primary endpoints included the objective response rate(ORR)and disease control rate(DCR),while secondary endpoints encompassed progression-free survival(PFS),overall survival(OS),and *** 66 patients analysed for efficacy,the best response,indicated by the ORR,was recorded at 50.0%(33/66)(95%CI,37.4–62.6%),with 33 patients achieving partial response(PR).Notably,the DCR was 95.5%(63/66,95%CI,87.3–99.1%).The median PFS(mPFS)and OS(mOS)were 8.8(95%CI,8.1–11.6),and 13.7(95%CI,12.4 to not reached)months,*** 3/4 treatment-related adverse events(TRAEs)were reported in 39.4%of patients(26/66).In prespecified exploratory analysis,patients with altered SWI/SNF complex had a poorer ***,low serum CA724 level,high T-cell recruitment,low Th17 cell recruitment,and high NK CD56dim cell scores at baseline were potential predicative biomarkers for more favourable *** conclusion,PAAG as a first-line therapy demonstrated tolerability with promising clinical efficacy for *** biomolecular findings identified in this study possess the potential to guide the precise clinical application of the triple-combo regimen.

关键词： CA724 prospective centre

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Dissecting the genetic basis of grain color and pre-harvest sprouting resistance in common wheat by association analysis

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Journal of Integrative Agriculture 2023年第9期22卷 2617-2631页

作者： yan Sheng-nan YU zhao-yu GAO Wei WANG Xu-yang CAO Jia-jia LU Jie MA Chuan-xi CHANG Cheng ZHANG Hai-ping Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow&Huai River Valley Ministry of Agriculture and Rural Affairs/College of AgronomyAnhui Agricultural UniversityHefei 230036P.R.China

Pre-harvest sprouting(PHS) adversely affects wheat quality and yield, and grain color(GC) is associated with PHS resistance. However, the genetic relationship between GC and PHS resistance remains unclear. In this study, 168 wheat varieties(lines) with significant differences in GC and PHS resistance were genotyped using an Illumina 90K iSelect SNP array. Genome-wide association study(GWAS) based on a mixed linear model showed that 67 marker-trait associations(MTAs) assigned to 29 loci, including 17 potentially novel loci, were significantly associated with GC, which explained 1.1–17.0% of the phenotypic variation. In addition, 100 MTAs belonging to 54 loci, including 31 novel loci, were significantly associated with PHS resistance, which accounted for 1.1–14.7% of the phenotypic variation. Subsequently, two cleaved amplified polymorphic sequences(CAPS) markers, 2B-448 on chromosome 2B and 5B-301 on chromosome 5B,were developed from the representative SNPs of the major common loci ***-2B.3/***-2B.4controlling GC/PHS resistance and PHS resistance locus ***-5B.4, respectively. Further validation in 171 Chinese mini-core collections confirmed significant correlations of the two CAPS markers with GC and PHS resistance phenotypes under different environments(P<0.05). Furthermore, the wheat public expression database, transcriptomic sequencing, and gene allelic variation analysis identified TraesCS5B02G545100, which encodes glutaredoxin, as a potential candidate gene linked to ***-5B.4. The new CAPS marker CAPS-356 was then developed based on the SNP(T/C) in the coding sequences(CDS) region of TraesCS5B02G545100. The high-density linkage map of the Jing 411/Hongmangchun 21 recombinant inbred lines(RILs) constructed based on specific locus amplified fragment sequencing markers showed that CAPS-356 collocated with a novel QTL for PHS resistance, supporting the role of TraesCS5B02G545100 as the potential candidate gene linked to ***-5B.4. These r

关键词： common wheat grain color PHS resistance GWAS 90K SNP CAPS marker

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