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ZBTB7A governs estrogen receptor alpha expression in breast cancer

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Journal of Molecular Cell Biology 2018年第4期10卷 273-284页

作者： Mary Ellen Molloy Monika Lewinska Amanda K. Williamson Thanh Thao Nguyen Gamze Kuser-Abali lu Gong Jiawei yan John B. Little Pier Paolo Pandolfi Zhi-min Yuan Department of Environmental Health John B. Little Center for Radiation Sciences Harvard T.H. Chan School of Public Health Boston MA 02215 USA Biotech Research and Innovation Centre Department of Health and Medical Sciences University of Copenhagen Copenhagen Denmark Cancer Genetics Program Beth Israel Deaconess Cancer Center Departments of Medicine and Pathology Beth Israel Deaconess Medical Center Harvard Medical School Boston MA 02215 USA

ZBTB7A, a member of the POZ/BTB and Krüppel （POK） family of transcription factors, has been shown to have a context-dependent role in cancer development and progression. The role of ZBTB7A in estrogen receptor alpha （ERα）-positive breast cancer is largely unknown. Approximately 70% of breast cancers are classified as ERα-positive. ERα carries out the biological effects of estrogen and its expression level dictates response to endocrine therapies and prognosis for breast cancer patients. In this study, we find that ZBTB7A transcriptionally regulates ERα expression in ERα-positive breast cancer cell lines by binding to the ESR1 promoter leading to increased transcription of ERα. Inhibition of ZBTB7A in ERα-positive cells results in decreased estrogen responsiveness as demonstrated by diminished estrogen-response element-driven luciferase reporter activity, induction of estrogen target genes, and estrogen-stimulated growth. We also report that ERα potentiates ZBTB7A expression via a post-translational mechanism, suggesting the presence of a positive feedback loop between ZBTB7A and ERα, conferring sensitivity to estrogen in breast cancer. Clinically, we find that ZBTB7A and ERα are often co-expressed in breast cancers and that high ZBTB7A expression correlates with improved overall and relapse-free survival for breast cancer patients. Importantly, high ZBTB7A expression predicts a more favorable outcome for patients treated with endocrine therapies. Together, these findings demonstrate that ZBTB7A contributes to the transcriptional program maintaining ERα expression and potentially an endocrine therapy-responsive phenotype in breast cancer.

关键词： ZBTB7A ERα breast cancer endocrine therapies

Effects of sediment load on the abrasion of soil aggregate and hydraulic parameters in experimental overland flow

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Journal of Integrative Agriculture 2020年第4期19卷 1117-1126页

作者： WANG Jun-guang YU Bing NI Shi-min GUO Zhong-lu CAI Chong-fa Key Laboratory of Arable Land Conservation(Middle and Lower Reaches of yangtze River) Ministry of Agriculture and Rural Affairs/College of Resources and EnvironmentHuazhong Agricultural UniversityWuhan 430070.P.R.China Hubei Water Resources Research Institute Wuhan 430070.P.R.China

The breakdown of soil aggregates under rainfall and their abrasion in overland flow are important processes in water erosion due to the production of more fine and transportable particles and,the subsequent significant effect on the erosion ***,little is known about the effects of sediment load on the soil aggregate abrasion and the relationship of this abrasion with some related hydraulic ***,the potential effects of sediment load on soil aggregate abrasion and hydraulic parameters in overland flow were investigated through a series of experiments in a 3.8-m-long hydraulic flume at the slope gradients of 8.7 and 26.8%,unit flow discharges from 2×10^-3 to 6×10^-3 m^2 s^-1,and the sediment concentration from 0 to 110 kg *** the aggregates from Ultisols developed Quaternary red clay,Central *** results indicated that discharge had the most significant(Plues followed a power function decrease with increasing friction factor or shear stress in the sediment-laden flow,indicating that friction factor is the best hydraulic parameter for prediction of soil aggregate abrasion under different sediment load conditio

关键词： soil aggregate sediment load hydraulic parameters overland flow

设施园艺电驱移动平台轮毂电机设计及其磁-热耦合性能

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农业工程学报 2024年第13期40卷 15-24页

作者：鲁应涛姜逸菲刘逸龙罗志涛郑恩来鲁植雄沈成汪小旵南京农业大学工学院南京210031 农业农村部南京农业机械化研究所南京210014

针对设施园艺作业场景下电驱移动平台的低速大扭矩牵引作业需求,该研究设计了一种减速内转子轴向磁通轮毂电机,建立电机的电磁有限元模型并分析其电磁和损耗特性。考虑到绕组电阻率受温度变化影响,构建基于热网络法的轮毂电机磁-热耦合... 详细信息

针对设施园艺作业场景下电驱移动平台的低速大扭矩牵引作业需求,该研究设计了一种减速内转子轴向磁通轮毂电机,建立电机的电磁有限元模型并分析其电磁和损耗特性。考虑到绕组电阻率受温度变化影响,构建基于热网络法的轮毂电机磁-热耦合改进模型。与台架试验结果相比,基于改进模型获得的转矩预测值较传统模型的精度提高了5.33%,验证了模型的正确性。利用改进模型分析了温度和相电流对电机转矩特性的影响,仿真结果表明,输出转矩随电机温度增加而降低;在0~20 A,电机平均输出转矩与相电流呈线性关系。试验结果表明,轮毂电机功率随转速增加而增大,峰值功率可达4.59 kW,而转矩基本保持恒定;在转速450~900 r/min、转矩300~600 N·m时,电机效率高达93.2%。研究结果可为轴向磁通电机初步设计与多场耦合影响下电机性能分析提供思路。

关键词：农业电机模型热网络法磁-热耦合温升特性

从“肺玄府-气液-络脉”理论分期辨治脓毒症急性肺损伤探析

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中国中医急症 2024年第4期33卷 612-616页

作者：黄仁刘俊刘敏商燕柏正平湖南省中医药研究院附属医院湖南长沙410006

笔者认为脓毒症急性肺损伤的病机演变与“肺玄府-气液-络脉”理论关系密切,宜分期辨治,早期宜清肺调玄、活血化饮、通利肺络,以阻断病情进展;炎性渗出期当泻肺调玄、活血利水、行气通络;肺水肿期需宣肺通玄、祛瘀逐饮、益气通络;肺实变... 详细信息

笔者认为脓毒症急性肺损伤的病机演变与“肺玄府-气液-络脉”理论关系密切,宜分期辨治,早期宜清肺调玄、活血化饮、通利肺络,以阻断病情进展;炎性渗出期当泻肺调玄、活血利水、行气通络;肺水肿期需宣肺通玄、祛瘀逐饮、益气通络;肺实变期应益肺开玄、祛瘀化痰、补虚通络。提倡早期干预,中西医结合综合治疗,尽早阻断病情恶性循环,降低死亡率,为中医药防治脓毒症急性肺损伤提供新的思路。

关键词：脓毒症急性肺损伤肺玄府脉络气液中医辨治

基于炮制前后质量一致性的清半夏炮制新工艺评价

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天然产物研究与开发 2024年第3期36卷 485-498页

作者：颜雨豪殷莉丽黎智蒋孟莲杨转珍万子玉赖月月李敏成都中医药大学药学院/现代中药产业学院省部共建西南特色中药资源国家重点实验室成都611137

为研究半夏与清半夏的质量传递关系,并评价新工艺蒸法所得炮制品与清半夏的质量一致性,本研究检测了生半夏及3种炮制品的浸出物、有机酸、多糖、核苷、蛋白质含量,使用主成分分析等方法比较3种炮制品的差异。结果表明,与生半夏相比,浸... 详细信息

为研究半夏与清半夏的质量传递关系,并评价新工艺蒸法所得炮制品与清半夏的质量一致性,本研究检测了生半夏及3种炮制品的浸出物、有机酸、多糖、核苷、蛋白质含量,使用主成分分析等方法比较3种炮制品的差异。结果表明,与生半夏相比,浸、煮制清半夏的浸出物减少,而蒸制品浸出物增加了264.76%;总酸、多糖含量均显著增加,其中蒸制品的增幅最大;核苷、蛋白质含量均大幅降低;综合分析方法均表明蒸制品与传统方法所得清半夏存在较大的质量差异。由此可知,浸、煮制清半夏质量一致性较好,而蒸法炮制品仍需对其加工工艺、药效做进一步研究。

关键词：半夏清半夏炮制工艺质量传递主成分分析一致性

Ethanol Increases Mechanical Pain Sensitivity in Rats via Activation of GABA_A Receptors in Medial Prefrontal Cortex

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Neuroscience Bulletin 2016年第5期32卷 433-444页

作者： Kai-Wen Geng Ting He Rui-Rui Wang Chun-Li Li Wen-Jun luo Fang-Fang Wu yan Wang Zhen Li Yun-Fei lu Su-min Guan Jun Chen Institute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders Tangdu Hospital The Fourth Military Medical University Xi'an 710038 China Key Laboratory of Brain Stress and Behavior People's Liberation Army Xi'an 710038 China Beijing Institute for Brain Disorders Beijing 100069 China School of Stomatology The Fourth Military Medical University Xi'an 710032 China

Ethanol is widely known for its ability to cause dramatic changes in emotion, social cognition, and behavior following systemic administration in *** neuroimaging studies suggest that alcohol dependence and chronic pain may share common mechanisms through amygdala-medial prefrontal cortex（m PFC） interactions. However, whether acute administration of ethanol in the m PFC can modulate pain perception is *** we showed that bilateral microinjections of ethanol into the prelimbic and infralimbic areas of the m PFC lowered the bilateral mechanical pain threshold for 48 h without influencing thermal pain sensitivity in adult ***, bilateral microinjections of artificial cerebrospinal fluid into the m PFC or bilateral microinjections of ethanol into the dorsolateral PFC（also termed as motor cortex area 1 in Paxinos and Watson＇s atlas of The Rat Brain. Elsevier Academic Press, Amsterdam, 2005） failed to do so, suggesting regional selectivity of the effects of ethanol. Moreover, bilateral microinjections of ethanol didnot change the expression of either pro-apoptotic（caspase-3 and Bax） or anti-apoptotic（Bcl-2） proteins, suggesting that the dose was safe and validating the method used in the current study. To determine whether c-aminobutyric acid A（GABA_A） receptors are involved in mediating the ethanol effects, muscimol, a selective GABA_Areceptor agonist, or bicuculline, a selective GABA_A receptor antagonist, was administered alone or co-administered with ethanol through the same route into the bilateral m PFC. The results showed that muscimol mimicked the effects of ethanol while bicuculline completely reversed the effects of ethanol and muscimol. In conclusion, ethanol increases mechanical pain sensitivity through activation of GABA_A receptors in the m PFC of rats.

关键词： Ethanol mPFC Pain perception GABAA receptor Empathy

Expression of Soluble Vascular Endothelial Growth Factor Receptor-2 and Its Effect on Proliferation of Vascular Endothelial Cells

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Chemical Research in Chinese Universities 2012年第2期28卷 245-248页

作者： WANG Song lu Jian-nan XU yan LIU lu-cheng WU Jiang SHAO Guo-guang SUN Xin The Seconal Hospital of Jilin University Changchun 130041P.R.China China-Japan Union Hospital of Jilin University Changchun 130033P.R.China The First Hospital of Jilin University Changchun 130021P.R.China

Vascular endothelial growth factors（VEGFs） respectively bind to each of three receptor tyrosine kinases（RTKs）,known as Flt-1,KDR and *** VEGFs and their respective families of receptor tyrosine kinases（VEGFRs） are critical proteins which can regulate vascular development during angiogenesis,we decided to explore the inhibitory effects of soluble kinase insert domain-containing receptor（sKDR） on endothelial cells and *** RNA was extracted from human umbilical vein endothelial cells（HUVEC）,and cDNA of extracellular domains 1―4 was amplified and recombined with pQE40 *** being expressed,affinity purified,renatured and analyzed by Western blot,the sKDR was assayed for its effects on endothelial cells by [3-（4,5-dimethylthiazol-2-yl）2,5-diphenyltetrazolium bromide]（MTT）,and on angiogenesis by chick chorioallantoic membrane（CAM） *** cDNA of 1150 bp was obtained via real-time polymerase chain reaction（RT-PCR）,and sKDR was expressed by pQE40 procaryotic expression system,purified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） analysis with only one band and proved by Western *** assay demonstrateds that sKDR could inhibit the VEGF-stimulated HUVEC from proliferation,and CAM experiment showed sKDR could block the VEGF-induced *** has the biological activity to bind with VEGF ligands and is a potential target for tumor anti-angiogenesis therapy.

关键词： Kinase insert domain-containing receptor(KDR) Angiogenesis Human umbilical vein endothelial cell(HUVEC) Chick chorioallantoic membrane(CAM)

Effect of interaction between loop bases and ions on stability of G-quadruplex DNA

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Chinese Physics B 2021年第1期30卷 605-611页

作者： Han-Zhen Qiao Yuan-yan Wu Yusong Tu Cong-min Ji College of Physics Science and Technology Yangzhou UniversityYangzhou 225009China

G-quadruplexes(GQs) are guanine-rich, non-canonical nucleic acid structures that play fundamental roles in biological processes. The topology of GQs is associated with the sequences and lengths of DNA, the types of linking loops, and the associated metal cations. However, our understanding on the basic physical properties of the formation process and the stability of GQs is rather limited. In this work, we employed ab initio, molecular dynamics(MD), and steered MD(SMD)simulations to study the interaction between loop bases and ions, and the effect on the stability of G-quadruplex DNA, the Drude oscillator model was used in MD and SMD simulations as a computationally efficient manner method for modeling electronic polarization in DNA ion solutions. We observed that the binding energy between DNA bases and ions(K^(+)/Na^(+))is about the base stacking free energies indicates that there will be a competition among the binding of M^(+)-base, H-bonds between bases, and the base-stacking while ions were bound in loop of GQs. Our SMD simulations indicated that the side loop inclined to form the base stacking while the loop sequence was Thy or Ade, and the cross-link loop upon the G-tetrads was not easy to form the base stacking. The base stacking side loop complex K+was found to have a good stabilization synergy. Although a stronger interaction was observed to exist between Cyt and K+, such an interaction was unable to promote the stability of the loop with the sequence Cyt.

关键词： G-quadruplex loop DNA base metalion

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氢燃料混合动力机车能量管理策略

内燃机与配件 2024年第10期 122-124页

作者：谢嘉欣郭婉露秦庆民中车株洲电力机车有限公司湖南株洲412000

氢燃料电池在国内外轨道交通领域的应用日益增加,能量管理策略作为氢燃料电池应用中的关键内容,受到越来越多的关注。本文针对某一种氢燃料电池混合动力机车的能量管理策略设计进行阐述,着重分析混合动力系统的能量平衡控制、分级电压... 详细信息

氢燃料电池在国内外轨道交通领域的应用日益增加,能量管理策略作为氢燃料电池应用中的关键内容,受到越来越多的关注。本文针对某一种氢燃料电池混合动力机车的能量管理策略设计进行阐述,着重分析混合动力系统的能量平衡控制、分级电压控制策略以及功率分配策略,基于运行工况及规则确定具体的控制策略,控制简单明确,可实现混合动力系统的稳定输出,充分发挥氢燃料电池和动力电池的优势,保障机车动态性能,满足不同牵引功率下机车运行需求,对推动混合动力机车能量管理技术的发展具有一定意义。

关键词：氢燃料电池混合动力系统能量管理