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Horticulture Research 2020年第1期7卷 2047-2052页

作者： Da-Gang Hu Cui-Hui Sun quan-yan zhang Kai-Di Gu Yu-Jin Hao National Key Laboratory of Crop Biology MOA Key Laboratory of Horticultural Crop Biology and Germplasm InnovationCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong 271018China

Basic helix−loop−helix(bHLH)domain-containing transcription factors are known for their roles in regulating various plant growth and developmental ***,we showed that MdbHLH3 from apple(Malus domestica)has multiple functions,modulating both anthocyanin biosynthesis and cell ***,we show that MdbHLH3 also regulates ethylene biosynthesis and leaf senescence by promoting the expression of dehydratase-enolasephosphatase complex 1(MdDEP1).Therefore,we propose a model whereby MdbHLH3 acts as a crucial factor that modulates anthocyanin biosynthesis and cell acidification in addition to fruit ripening and leaf senescence by regulating distinct target genes.

关键词： Basic processes complex

MdbHLH3 modulates apple soluble sugar content by activating phosphofructokinase gene expression

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Journal of Integrative Plant Biology 2022年第4期64卷 884-900页

作者： Jian-Qiang Yu Kai-Di Gu Li-Li zhang Cui-Hui Sun quan-yan zhang Jia-Hui Wang Chu-Kun Wang Wen-yan Wang Meng-Chi Du Da-Gang Hu National Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTai'an 271018China College of Resources and Environment Linyi UniversityLinyi 276005China MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation Tai'an 271018China Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency Tai'an 271018China

Sugars are involved in plant growth,fruit quality,and signaling ***,understanding the mechanisms involved in soluble sugar accumulation is essential to understand fruit ***,we report that Md PFPβ,a pyrophosphatedependent phosphofructokinase gene,regulates soluble sugar accumulation by enhancing the photosynthetic performance and sugar-metabolizing enzyme activities in apple(Malus domestica Borkh.).Biochemical analysis revealed that a basic helix-loop-helix(b HLH)transcription factor,Mdb HLH3,binds to the Md PFPβpromoter and activates its expression,thus promoting soluble sugar accumulation in apple *** addition,Md PFPβoverexpression in tomato influenced photosynthesis and carbon metabolism in the ***,we determined that Mdb HLH3 increases photosynthetic rates and soluble sugar accumulation in apple by activating Md PFPβ*** results thus shed light on the mechanism of soluble sugar accumulation in apple leaves and fruit:Mdb HLH3 regulates soluble sugar accumulation by activating Md PFPβgene expression and coordinating carbohydrate allocation.

关键词： apple bHLH fructose 6-phosphate fruit quality phosphofructokinase soluble sugars

Autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells

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Asian Journal of Andrology 2011年第6期13卷 881-888页

作者： Wei-Ren Li Liang Chen Zhi-Jie Chang Hua Xin Tao Liu yan-quan zhang Guang-Yong Li Feng Zhou yan-Qing Gong Zhe-Zhu Gao Zhong-Cheng Xin Andrology Center Peking University First Hospital Peking University Beijing 100034 China Department of Urology China Meitan General Hospital Beijing 100028 China Department of Biological Sciences and Biotechnology School of Medicine Tsinghua University Beijing 100084 China

Late-onset hypogonadism （LOH） is closely related to secondary androgen deficiency in aged males, but the mechanism remains unclear. In this study, we found that reduced testosterone production in aged rat Leydig cells is associated with decreased autophagic activity. Primary rat Leydig cells and the TM3 mouse Leydig cell line were used to study the effect of autophagic deficiency on Leydig cell testosterone production. In Leydig cells from young and aged rats, treatment with wortmannin, an autophagy inhibitor, inhibited luteinising hormone （LH）-stimulated steroidogenic acute regulatory （STAR） protein expression and decreased testosterone production. In contrast, treatment with rapamycin, an autophagy activator, enhanced LH-stimulated steroidogenesis in Leydig cells from aged, but not young, rats. Intracellular reactive oxygen species （ROS） levels were increased in both young and aged Leydig cells treated with wortmannin but decreased only in aged Leydig cells treated with rapamycin. Furthermore, an increased level of ROS, induced by H2O2, resulted in LH-stimulated steroidogenic inhibition. Finally, knockdown of Beclin 1 decreased LH-stimulated StAR expression and testosterone production in TM3 mouse Leydig cells, which were associated with increased intracellular ROS level. These results suggested that autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells, which might be influenced by intracellular ROS levels.

关键词： ageing autophagy late onset hypogonadism Leydig cell reactive oxygen species testosterone

Application and progress of artificial intelligence technology in the segmentation of hyperreflective foci in OCT images for ophthalmic disease research

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International Journal of Ophthalmology(English edition) 2024年第6期17卷 1138-1143页

作者： Jia-Ning Ying Hu Li yan-yan zhang Wen-Die Li quan-Yong Yi Ningbo Eye Hospital Wenzhou Medical UniversityNingbo 315042Zhejiang ProvinceChina Health Science Center Ningbo UniversityNingbo 315211Zhejiang ProvinceChina

With the advancement of retinal imaging,hyperreflective foci(HRF)on optical coherence tomography(OCT)images have gained significant attention as potential biological biomarkers for retinal ***,these biomarkers,represented by HRF,present pose challenges in terms of localization,quantification,and require substantial time and *** recent years,the progress and utilization of artificial intelligence(AI)have provided powerful tools for the analysis of biological *** technology enables use machine learning(ML),deep learning(DL)and other technologies to precise characterization of changes in biological biomarkers during disease progression and facilitates quantitative *** on ophthalmic images,AI has significant implications for early screening,diagnostic grading,treatment efficacy evaluation,treatment recommendations,and prognosis development in common ophthalmic ***,it will help reduce the reliance of the healthcare system on human labor,which has the potential to simplify and expedite clinical trials,enhance the reliability and professionalism of disease management,and improve the prediction of adverse *** article offers a comprehensive review of the application of AI in combination with HRF on OCT images in ophthalmic diseases including age-related macular degeneration(AMD),diabetic macular edema(DME),retinal vein occlusion(RVO)and other retinal diseases and presents prospects for their utilization.

关键词： artificial intelligence deep learning hyperreflective foci image analysis

Aerial refueling scheduling of multi-receiver and multi-tanker under spatial-temporal constraints for forest firefighting

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Chinese Journal of Aeronautics 2024年第5期37卷 71-91,I0001页

作者： Jing HUANG Jiaqi XING Jinrui REN quan quan Youmin zhang Department of Information and Control Engineering Xi’an University of TechnologyXi’an 710048China Jiujiang Measuring Test Technology Research Institute Jiujiang 332000China School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China School of Automation Xi’an University of Posts&TelecommunicationsShaanxiXi’an 710121China Department of Mechanical Industrial and Aerospace EngineeringConcordia UniversityMontreal H3G 1M8Canada

Forest fires pose a significant threat to human life and property,so the utilization of unmanned aircraft systems provides new ways for forest *** the constrained load capacities of these aircraft,aerial refueling becomes crucial to extend their operational time and *** order to address the complexities of firefighting missions involving multi-receiver and multi-tanker deployed from various airports,first,a fuel consumption calculation model for aerial refueling scheduling is established based on the receiver ***,two distinct methods,including an integrated one and a decomposed one,are designed to address the challenges of establishing refueling airspace and allocating tasks for *** methods aim to optimize total fuel consumption of the receivers and tankers within the aerial refueling scheduling *** optimization problem is established as nonlinear optimization models along with *** integrated method seamlessly combines refueling rendezvous point scheduling and tanker task allocation into unified *** has a complete solution space and excels in optimizing total fuel *** decomposed method,through the separation of rendezvous point scheduling and task allocation,achieves a reduced computational ***,this comes at the cost of sacrificing optimality by excluding specific feasible ***,numerical simulations are carried out to verify the feasibility and effectiveness of the proposed *** simulations yield insights crucial for the practical engineering application of both the integrated and decomposed methods in real-world *** comprehensive approach aims to enhance the efficiency of forest firefighting operations,mitigating the risks posed by forest fires to human life and property.

关键词： Aerial refueling Scheduling Planning Spatial-temporal constraint Firefighting

Initial costates derived by near-optimal reference sequence and least-squares method

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Chinese Journal of Aeronautics 2024年第5期37卷 377-391页

作者： Shaozhao LU Yao zhang quan HU School of Aerospace Engineering Beijing Institute of TechnologyBeijing 100081China

In this paper,we present a novel initial costates solver for initializing time-optimal trajectory problems in relative motion with continuous low *** proposed solver consists of two primary components:training a Multilayer Perceptron(MLP)for generating reference sequence and Time of Flight(TOF)to the target,and deriving a system of linear algebraic equations for obtaining the initial *** overcome the challenge of generating training samples for the MLP,the backward generation method is proposed to obtain five different training *** training database and sample form are determined by analyzing the input and output correlation using the Pearson correlation *** best-performing MLP is obtained by analyzing the training results with various hyper-parameter combinations.A reference sequence starting from the initial states is obtained by integrating forward with the near-optimal control vector from the output of ***,a system of linear algebraic equations for estimating the initial costates is derived using the reference sequence and the necessary conditions for *** results demonstrate that the proposed initial costates solver improves the convergence ratio and reduce the function calls of the shooting ***,Monte-Carlo simulation illustrates that the initial costates solver is applicable to different initial velocities,demonstrating excellent generalization ability.

关键词： Relative motion Expanding training database Multilayer perceptron Initial costates Least-squares method

Effect of Al content on modification behavior of non-metallic inclusions in high-strength steel treated with rare earth

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Journal of Iron and Steel Research International 2024年第8期31卷 1886-1899页

作者： Zhan-peng Tie Xue-yuan Jiang Hai-yan Tang ·Gen Li Yu-hang Wang Jia-quan zhang School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China Continuous Casting Business Section CISDI Engineering Co.Ltd.Chongqing 401122China Special Steel Institute Central Iron and Steel Research Institute Co.Ltd.Beijing 100081China

The influence of Al content(0.0053,0.0171,and 0.0578 wt.%)on the modification behavior of non-metallic inclusions in 20CrMoVTiB steel treated with rare earth elements was studied through high-temperature experiments and thermodynamic *** results showed that the modification products varied with the Al content in steel under 0.01 wt.%of Ce *** formation sequence of typical rare earth inclusions in steel with the increase in Al content was Ce_(2)O_(3)→CeAlO_(3)→CeAl_(11)O_(18),and the final stable products were highly Al content *** the Al content was 0.0053 wt.%,the stable phase in steel was Ce2O3;while the[Al]reached 0.0171 wt.%,the stable phase became CeA1O_(3).As the A1 content reached 0.0578 wt.%,CeAl_(11)O_(18) became the stable *** a result,increasing the Al content could inhibit the precipitation of Ce_(2)O_(3) inclusions in steel while promote the formation of CeAIO3 and CeAl_(11)O_(18) *** addition,both Ca treatment and Ce treatment could effectively refine the size of inclusions in steel by changing their ***,the feeding amount of Ca wire into molten steel should be appropriately reduced under the condition of adding Ce simultaneously,which is expected to be beneficial for an improved Ce treatment effect.

关键词： Rare earth Al content Steel Inclusion Thermodynamics Ca treatment

BTB-BACK-TAZ domain protein MdBT2-mediated MdMYB73 ubiquitination negatively regulates malate accumulation and vacuolar acidification in apple

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Horticulture Research 2020年第1期7卷 827-838页

作者： quan-yan zhang Kai-Di Gu Jia-Hui Wang Jian-Qiang Yu Xiao-Fei Wang Shuai zhang Chun-Xiang You Da-Gang Hu Yu-Jin Hao National Key Laboratory of Crop Biology Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong 271018China College of Chemistry and Material Science Shandong Agricultural UniversityTai-AnShandong 271018China

As an important primary metabolite,malate plays a key role in regulating osmotic pressure,pH homeostasis,stress tolerance,and fruit quality of *** R2R3-MYB transcription factor(TF)MdMYB73 was identified as a protein that plays a critical role in determining malate accumulation and vacuolar acidification by directly regulating the transcription of aluminum-activated malate transporter 9(MdALMT9),vacuolar ATPase subunit A(MdVHA-A),and vacuolar pyrophosphatase 1(MdVHP1)in *** addition,the bHLH TF MdCIbHLH1 interacts with MdMYB73 and enhances the transcriptional activity of *** previous studies demonstrated that the BTB-BACK-TAZ domain protein MdBT2 can degrade MdCIbHLH1 to influence malate accumulation and vacuolar ***,the potential upstream regulators of MdMYB73 are currently *** this study,we found that MdBT2 directly interacts with and degrades MdMYB73 through the ubiquitin/26S proteasome pathway to regulate malate accumulation and vacuolar acidification.A series of functional assays with apple calli and fruit showed that MdBT2 controls malate accumulation and vacuolar acidification in an MdMYB73-dependent ***,our findings shed light on the mechanism by which the BTB-BACK-TAZ domain protein MdBT2 regulates malate accumulation and vacuolar acidification by targeting MdMYB73 and MdCIbHLH1 for ubiquitination in *** information may help guide traditional breeding programs and fruit tree molecular breeding,and lead to improvements in fruit quality and stress tolerance.

关键词： malate ubiquitin homeostasis

Self-Sustaining of Critical Park Microgrids Integrating Mobile Emergency Generators Subjective to Major Outage

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CSEE Journal of Power and Energy Systems 2024年第4期10卷 1441-1453页

作者： quan Sui Lei zhang School of Electrical and Information Engineering Zhengzhou UniversityZhengzhou 450001China School of Electrical and New Energy Three Gorges UniversityYichang 443002China

In the event of a major power outage,critical park microgrids(PMGs)could be self-sustaining if mobile emergency generators(MEGs)are stationed to share ***,the need for privacy protection and the value of flexible power support on minute-time scales have not been given enough *** address the problem,this paper proposes a new self-sustaining strategy for critical PMGs integrating ***,to promote the cooperation between PMG and MEG,a bi-level benefit distribution mechanism is designed,where the participants'multiple roles and contributions are identified,and good behaviors are also ***,to increase the alliance benefits,three loss coordination modes are presented to guide the power exchange at the minute level between the MEG and PMG,considering the volatility of renewable generation and *** this basis,a multi-time scale power-energy scheduling strategy is formulated via the alternating direction method of multipliers(ADMM)to coordinate the PMG and ***,a dimensionality reduction technology is designed to equivalently simplify the optimization problem to facilitate the adaptive-step-based ADMM *** studies indicate that the proposed strategy achieves the self-sustaining of PMGs integrating MEGs while increasing the economy by no less than 3.1%.

关键词： Distributed optimization loss coordination mode mobile emergency generator park microgrid power-energy self-sustaining privacy protection

Monotropein Induced Apoptosis and Suppressed Cell Cycle Progression in Colorectal Cancer Cells

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Chinese Journal of Integrative Medicine 2024年第1期30卷 25-33页

作者： GAO quan LI Lin zhang Qi-man SHENG Qin-song zhang Ji-liang JIN Li-jun SHANG Rui-yan School of Pharmacy and Department of Medical Oncology the Affiliated Hospital of Hangzhou Normal UniversityHangzhou Normal UniversityHangzhou(311121)China Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines Engineering Laboratory of Development and Application of Traditional Chinese MedicinesCollaborative Innovation Center of Traditional Chinese Medicines of Zhejiang ProvinceHangzhou Normal UniversityHangzhou(311121)China Department of Colorectal Surgery the First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouChina Beijing Tong Ren Tang Chinese Medicine Co. Ltd.Beijing(100000)China Department of Traditional Chinese Medicine Hangzhou Shangcheng District People's HospitalHangzhou(310006)China Department of Gynecology Hangzhou Women's HospitalHangzhou(310008)China

Objective: To determine whether monotropein has an anticancer effect and explore its potential mechanisms against colorectal cancer(CRC) through network pharmacology and molecular docking combined with experimental verification. Methods: Network pharmacology and molecular docking were used to predict potential targets of monotropein against CRC. Cell counting kit assay, plate monoclonal assay and microscopic observation were used to investigate the antiproliferative effects of monotropein on CRC cells HCT116, HT29 and LoVo. Flow cytometry and scratch assay were used to analyze apoptosis and cell cycle, as well as cell migration, respectively in HCT116, HT29, and LoVo cells. Western blotting was used to detect the expression of proteins related to apoptosis, cell cycle, and cell migration, and the expression of proteins key to the Akt pathway. Results: The Gene Ontology and Reactome enrichment analyses indicated that the anticancer potential of monotropein against CRC might be involved in multiple cancer-related signaling pathways. Among these pathways, RAC-beta serine/threonine-protein kinase(Akt1, Akt2), cyclin-dependent kinase 6(CDK6), matrix metalloproteinase-9(MMP9), epidermal growth factor receptor(EGFR), cell division control protein 42 homolog(CDC42) were shown as the potential anticancer targets of monotropein against CRC. Molecular docking suggested that monotropein may interact with the 6 targets(Akt1, Akt2, CDK6, MMP9, EGFR, CDC42). Subsequently, cell activity of HCT116, HT29 and LoVo cell lines were significantly suppressed by monotropein(P<0.05). Furthermore, our research revealed that monotropein induced cell apoptosis by inhibiting Bcl-2 and increasing Bax, induced G_1–S cycle arrest in colorectal cancer by decreasing the expressions of CyclinD1, CDK4 and CDK6, inhibited cell migration by suppressing the expressions of CDC42 and MMP9(P<0.05), and might play an anticancer role through Akt signaling pathway. Conclusions: Monotropein exerts its antitumor

关键词： monotropein Chinese medicine anticancer activity colorectal cancer cells network pharmacology