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分离富集磷酸化/糖基化蛋白质/肽的磁固相萃取新材料

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分析测试学报 2025年第1期44卷 1-11页

作者：鲁艳张森张锋葛鸿延庄琬月乔俊琴练鸿振生命分析化学国家重点实验室南京大学化学化工学院南京大学现代分析中心江苏南京210023

蛋白质磷酸化和糖基化是蛋白质两种主要的翻译后修饰过程。磷酸化和糖基化蛋白质在细胞的各个生命周期内起着关键的作用,对于临床疾病的早期诊断等也具有重要的意义。但由于它们在实际生物样品中含量低、基质干扰大,选择合适的方法对其... 详细信息

蛋白质磷酸化和糖基化是蛋白质两种主要的翻译后修饰过程。磷酸化和糖基化蛋白质在细胞的各个生命周期内起着关键的作用,对于临床疾病的早期诊断等也具有重要的意义。但由于它们在实际生物样品中含量低、基质干扰大,选择合适的方法对其进行分离富集成为蛋白质组学研究中决定性的步骤之一。磁固相萃取(MSPE)是以磁性纳米颗粒为吸附剂的固相萃取技术(SPE),较之常规SPE具有操作简单、环境友好、材料易于回收利用等优势,因而被广泛应用于磷酸化和糖基化蛋白/肽段等分析前的分离富集。该文对近5年文献中用于磷酸化和糖基化蛋白质分离富集的MSPE材料进行系统的归纳和评述,并对此类分离富集材料的未来发展方向进行了展望和探讨。

关键词：磷酸化蛋白质糖基化蛋白质磁固相萃取磁性纳米颗粒

19.3% Efficiency ternary organic solar cells enabled by the alkyl side-chain effect of guest non-fullerene acceptors

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Science China Chemistry 2024年第8期67卷 2686-2693页

作者： Hongqian Wang Sijian Wu Daobin yang Xueliang Yu Shuncheng yang Pengfei Ding Pengyu yan Zaifei Ma Jianfeng Zhang Ziyi ge School of Materials Science and Chemical Engineering Ningbo UniversityNingbo 315211China Zhejiang Engineering Research Center for Energy Optoelectronic Materials and Devices Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Center for Advanced Low-dimension MaterialsCollege of Materials Science and EngineeringDonghua UniversityShanghai 201620China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

The effect of side-chain engineering of conjugated molecules on the morphology and device performance in binary organic solar cells has been widely investigated. However, this relationship has hardly been studied in the guest components of ternary organic solar cells. In this study, a family of non-fullerene guest acceptors, namely XY-3, XY-5 and XY-7, with hydrogen substituent,straight and branched alkyl chains on the bithiophene units, respectively, were designed and synthesized to understand their effects on aggregation properties and device performance. The straight and branched alkyl chains on the bithiophene units result in sightly blue-shifted absorption compared to the hydrogen substituent and the XY-7 demonstrates the most appropriate phase separation scale and the most balanced charge transport. Consequently, the OSCs based on D18:e C9:XY-7 achieve a high shortcircuit current density(JSC) and fill factor(FF), while maintaining the enhancement of the open-circuit voltage(VOC) achieving an efficiency of 19.32%, exceeding those of D18:e C9, D18:e C9:XY-3, D18:e C9:XY-5(PCE:18.28%, 19.04%, 18.75%, respectively). These results highlight that the side-chain engineering of Y series non-fullerene acceptors as the guest acceptors has great potential in optimizing morphology properties and promoting photovoltaic performance.

关键词： organic solar cells side chain engineering ternary strategy non-fullerene energy losses

Trace Projection Transformation： a new method for measurement of debris flow surface velocity fields

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Frontiers of Earth Science 2016年第4期10卷 761-771页

作者： yan yan Peng CUI Xiaojun GUO Yonggang ge Key Laboratory of Mountain Surface Process and Hazards/Institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu 610041 China University of Chinese Academy of Sciences Beijing 100049 China Center for Excellence in Tibetan Plateau Earth Sciences Chinese Academy of Sciences Beijing 100101 China

Spatiotemporal variation of velocity is impor- tant for debris flow dynamics. This paper presents a new method, the trace projection transformation, for accurate, non-contact measurement of a debris-flow surface velocity field based on a combination of dense optical flow and perspective projection transformation. The algorithm for interpreting and processing is implemented in C ＋＋ and realized in Visual Studio 2012. The method allows quantitative analysis of flow motion through videos from various angles （camera positioned at the opposite direction of fluid motion）. It yields the spatiotemporal distribution of surface velocity field at pixel level and thus provides a quantitative description of the surface processes. The trace projection transformation is superior to conventional measurement methods in that it obtains the full surface velocity field by computing the optical flow of all pixels. The result achieves a 90% accuracy of when comparing with the observed values. As a case study, the method is applied to the quantitative analysis of surface velocity field of a specific debris flow.

关键词： debris flow surface velocity field spatiotem-poral variation dense optical flow perspective projectiontransformation

Stability Analysis and Control of DC Distribution System with Electric Vehicles

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Energy Engineering 2023年第3期120卷 633-647页

作者： Zhijie Zheng Song Zhang Xiaolei Zhang Bo yang Fang yan Xiaoning ge State Grid Shandong Electric Power Company Economic and Technological Research Institute JinanChina State Grid Shandong Electric Power Company Limited JinanChina State Nuclear Electric Power Planning Design&Research Institute Company Limited BeijingChina

The DC distribution network system equipped with a large number of power electronic equipment exhibits weak damping characteristics and is prone to low-frequency and high-frequency unstable *** current interpretation of the oscillation mechanism has not been ***,this paper established the complete statespace model of the distribution system consisting of a large number of electric vehicles,characteristic equation of the distribution network system is derived by establishing a state-space model,and simplified reduced-order equations describing the low-frequency oscillation and the high-frequency oscillation are ***,based on eigenvalue analysis,the oscillation modes and the influence of the key system parameters on the oscillation mode are ***,impacts of key factors,such as distribution network connection topology and number of dynamic loads,have been discussed to suppress oscillatory instability caused by inappropriate design or dynamic ***,using the DC distribution example system,through model calculation and time-domain simulation analysis,the correctness of the aforementioned analysis is verified.

关键词： DC distribution network system oscillation instability reduced-order equivalent model damping control sensitivity analysis

Rheological, Thermal and Mechanical Properties of Biodegradable Poly(propylene carbonate)/Polylactide/Poly(1,2-propylene glycol adipate) Blown Films

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Chinese Journal of Polymer Science 2015年第12期33卷 1702-1712页

作者： yan-ping Hao Hui-li yang Gui-bao Zhang 张会良 ge Gao Li-song Dong College of Chemistry Jilin University Key Laboratory of Polymer Ecomaterials Chinese Academy of Sciences Changchun Institute of Applied Chemistry

Poly（propylene carbonate）（PPC） was blended with polylactide （PLA） and poly（1,2-propylene glycol adipate）（PPA） using a twin screw extruder. Then the PPC/PLA/PPA films were prepared using the blown film technique. DMA results showed that PPA could act as a plasticizer and improve the miscibility between PPC and PLA. Crystal morphology displayed that blending PLA with the amorphous PPC led to a decrease of the spherulite size of PLA. The results of mechanical tests indicated that PPC-rich films showed high elongation at break and PLA-rich films showed high tear strength and good optical properties. The content of PPC and PLA significantly affected the physical properties of the films. With increasing PPC content, the melt strengths of the PPC/PLA/PPA films were enhanced. These findings contributed to the biodegradable materials application for designing and manufacturing polymer packaging.

关键词： Poly(propylene carbonate) Polylactide Plasticizer Blown films.

DNN-Based Speech Enhancement Using Soft Audible Noise Masking for Wind Noise Reduction

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China Communications 2018年第9期15卷 235-243页

作者： Haichuan Bai Fengpei ge Yonghong yan Key Laboratory of Speech Acoustics and Content Understanding Institute of Acoustics Chinese Academy of SciencesBeijing 100190 China University of Chinese Academy of Sciences Beijing 100049 China Xinjiang Laboratory of Minority Speech and Language Information Processing Xinjiang Technical Institute of Physics and ChemistryChinese Academy of Sciences Urumqi 830011 China

This paper presents a deep neural network(DNN)-based speech enhancement algorithm based on the soft audible noise masking for the single-channel wind noise reduction. To reduce the low-frequency residual noise, the psychoacoustic model is adopted to calculate the masking threshold from the estimated clean speech spectrum. The gain for noise suppression is obtained based on soft audible noise masking by comparing the estimated wind noise spectrum with the masking threshold. To deal with the abruptly time-varying noisy signals, two separate DNN models are utilized to estimate the spectra of clean speech and wind noise components. Experimental results on the subjective and objective quality tests show that the proposed algorithm achieves the better performance compared with the conventional DNN-based wind noise reduction method.

关键词： wind noise reduction speech enhancement soft audible noise masking psychoacoustic model deep neural network

Effects of high modes on the wind-induced response of super high-rise buildings

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Earthquake Engineering and Engineering Vibration 2012年第3期11卷 427-434页

作者： Feng Ruoqiang Guirong yan ge Jinming The Key Laboratory of Concrete and Prestressed Concrete Structures of Ministry of Education Southeast University Nanjing 210096 China State Key Lab of Subtropical Building Science South China University of Technology China Department of Civil Engineering University of Texas at El Paso E1 Paso Texas 79968 USA

For super high-rise buildings, the vibration period of the basic mode is several seconds, and it is very close to the period of the fluctuating wind. The damping of super high-rise buildings is low, so super high-rise buildings are very sensitive to fluctuating wind. The wind load is one of the key loads in the design of super high-rise buildings. It is known that only the basic mode is needed in the wind-response analysis of tall buildings. However, for super high-rise buildings, especially for the acceleration response, because of the frequency amplification of the high modes, the high modes and the mode coupling may need to be considered. Three typical super high-rise projects with the SMPSS in wind tunnel tests and the random vibration theory method were used to analyze the effect of high modes on the wind-induced response. The conclusions can be drawn as follows. First, for the displacement response, the basic mode is dominant, and the high modes can be neglected. Second, for the acceleration response, the high modes and the mode coupling should be considered. Lastly, the strain energy of modes can only give the vibration energy distribution of the high-rise building, and it cannot describe the local wind-induced vibration of high-rise buildings, especially for the top acceleration response.

关键词： super high-rise building wind tunnel test mode couple wind-induced response

Capacities and Failure Modes of Suction Bucket Foundation with Internal Bulkheads

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Journal of Ocean University of China 2017年第4期16卷 627-634页

作者： XIAO Zhong ge Borui WANG yan State Key Laboratory of Hydraulic Engineering Simulation and Safety Tianjin University Tianjin 300072 P. R. China

Suction bucket foundations can be divided into four compartments by cruciform internal bulkheads,thereby yielding better capacity in certain conditions than those without internal *** yet,no systematic study has been conducted regarding the effects of cruciform internal bulkheads on the capacities of suction bucket *** this study,we established a large number of finite element models of suction bucket foundations with and without cruciform internal bulkheads and of solid embedded circular *** found the uniaxial capacities and failure modes of suction bucket foundations with various depth ratios to remain basically unaffected by internal bulkheads in uniform ***,in inhomogeneous clay with high strength heterogeneity,we observed the uniaxial moment and horizontal capacities and corresponding failure modes of suction bucket foundations with a low depth ratio to be obviously affected by internal *** this case,the uniaxial moment capacities,in particular,as well as the horizontal capacities of suction bucket foundations with cruciform internal bulkheads become obviously greater than those without internal *** combined loading,we found the failure envelopes of suction bucket foundations with and without cruciform internal bulkheads and of solid circular foundation to also be basically consistent in uniform ***,in inhomogeneous clay with high strength heterogeneity,cruciform internal bulkheads can obviously change the shapes of the failure envelopes of bucket foundations with a small depth *** conclude that when the acting vertical load or foundation depth is relatively small,suction bucket foundations with cruciform internal bulkheads can be subjected to larger moment and horizontal loads in soft clays with high strength heterogeneity.

关键词： capacity bucket foundation internal bulkheads undrained clay failure modes

MicroRNA-208a silencing against myocardial ischemia/reperfusion injury mediated by reversibly camouflaged biomimetic nanocomplexes

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Nano Research 2023年第8期16卷 11176-11185页

作者： Jianhui Lu Jiaheng Zhang Wen yan Chenglong ge yang Zhou Rongying Zhu Shanzhou Duan Lichen Yin Yongbing Chen Department of Thoracic Surgery Suzhou Key Laboratory of Thoracic OncologyThe Second Affiliated Hospital of Soochow UniversitySuzhou 215004China Center of Stomatology The Second Affiliated Hospital of Soochow UniversitySuzhou 215004China Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices Institute of Functional Nano&Soft Materials(FUNSOM)Collaborative Innovation Center of Suzhou Nano Science&TechnologySoochow UniversitySuzhou 215123China

MicroRNA-208a(miR-208a)plays critical roles in the severe fibrosis and heart failure post myocardial ischemia/reperfusion(IR)***-208a inhibitor(mI)with complementary RNA sequence can silence the expression of miR-208a,while it is challenging to achieve efficient and myocardium-targeted ***,biomimetic nanocomplexes(NCs)reversibly coated with red blood cell membrane(RM)were developed for the myocardial delivery of *** construct the NCs,membrane-penetrating helical polypeptide(PG)was first adopted to condense mI and form the cationic inner core,which subsequently adsorbed catalase(CAT)via electrostatic interaction followed by surface coating with *** membrane-coated NCs enabled prolonged blood circulation after systemic administration,and could accumulate in the injured myocardium via passive *** the oxidative microenvironment of injured myocardium,CAT decomposed H_(2)O_(2)to produce O_(2)bubbles,which drove the shedding of the outer RM to expose the positively charged inner core,thus facilitated effective internalization by cardiac *** on the combined contribution of mI-mediated miR-208a silencing and CAT-mediated alleviation of oxidative stress,NCs effectively ameliorated the myocardial microenvironment,hence reducing the infarct size as well as fibrosis and promoting recovery of cardiac *** study provides an effective strategy for the cytosolic delivery of nucleic acid cargoes in the myocardium,and it renders an enlightened approach to resolve the blood circulation/cell internalization dilemma of cell membrane-coated delivery systems.

关键词： myocardial ischemia/reperfusion(IR)injury microRNA-208a silencing red blood cell membrane reversible membrane coating H_(2)O_(2)responsiveness membrane-penetratingα-helical polypeptide