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9V型肺炎球菌多糖蛋白结合物中游离多糖去除方法的筛选及优化

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中国生物制品学杂志 2024年第5期37卷 593-597页

作者：陈倩陈中伟马浩亮詹远枫闫瑾孙述学北京成大天和生物科技有限公司北京101111

目的筛选一种有效去除9V型肺炎球菌(Pn9V)多糖蛋白结合物中游离多糖的方法,并进行工艺优化及稳定性验证,以期用于多价肺炎结合疫苗的制备。方法制备Pn9V-CRM197结合物,分别采用分子筛(Sepharose 4FF)、离子交换层析(DEAE Sepharose FF)... 详细信息

目的筛选一种有效去除9V型肺炎球菌(Pn9V)多糖蛋白结合物中游离多糖的方法,并进行工艺优化及稳定性验证,以期用于多价肺炎结合疫苗的制备。方法制备Pn9V-CRM197结合物,分别采用分子筛(Sepharose 4FF)、离子交换层析(DEAE Sepharose FF)、疏水层析(GP-Butyl)及硫酸铵(ammonium sulfate,AS)盐析法去除结合物中的游离多糖,通过比较游离多糖百分比、多糖回收率及蛋白回收率筛选最适方法,并对确定的方法进行工艺优化。连续制备3批结合物,验证工艺的稳定性。结果疏水层析和离子交换层析均可将Pn9V-CRM197结合物的游离多糖降至5%以下,疏水层析多糖及蛋白回收率(34.7%和50.1%)高于离子交换层析(16.2%和25.7%),选择疏水层析去除结合物中的游离多糖。优化的工艺为1.1 mol/L AS上样,注射用水洗脱,层析载量为1.81 mg蛋白/mL填料。连续制备的3批结合物游离多糖去除率、多糖回收率及蛋白回收率良好,且批间一致性高;2~8℃放置6个月,结合物稳定性良好,(25±2)℃放置6个月,游离多糖百分比显著增加(F=5.83 e-32,P=0.003),且>25%,不适宜高温保存。结论建立了有效去除Pn9V蛋白结合物中游离多糖的疏水层析工艺,该工艺稳定性良好,也为其他型别肺炎球菌多糖蛋白结合物游离多糖的去除提供了参考。

关键词：肺炎球菌多糖蛋白结合物游离多糖疏水层析

赣北与赣南地区典型中温温泉水文地球化学特征

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矿物岩石地球化学通报 2024年第3期43卷 521-532页

作者：施兴华白细民孙占学刘金辉叶海龙庞龙龙关燕鹤陈功新东华理工大学核资源与环境国家重点实验室南昌330013 东华理工大学水资源与环境工程学院南昌330013 江西省勘察设计研究院有限公司南昌330224 东华理工大学地球物理与测控技术学院南昌330013

为践行“双碳”目标,助力中国地热资源的可持续开发利用,本文采用同位素手段和多矿物平衡模拟法,研究了赣北的白岭温泉与赣南的汤湖温泉和白石温泉的水化学特征。结果表明,白岭温泉(85℃)为弱碱性HCO_(3)-Na型硅-氟水,其总溶解固体(TDS... 详细信息

为践行“双碳”目标,助力中国地热资源的可持续开发利用,本文采用同位素手段和多矿物平衡模拟法,研究了赣北的白岭温泉与赣南的汤湖温泉和白石温泉的水化学特征。结果表明,白岭温泉(85℃)为弱碱性HCO_(3)-Na型硅-氟水,其总溶解固体(TDS)含量、H_(2)SiO_(3)含量和主要离子浓度均低于汤湖温泉(89℃,弱碱性HCO_(3)-Na型硅-氟水)和白石温泉(85℃,弱碱性SO_(4)-HCO_(3)-Na型硅-氟水)。三个温泉虽均来源于大气降水,但汤湖温泉和白石温泉水的地下滞留时间更长,而白岭温泉的循环深度更大。白岭温泉和汤湖温泉的水岩作用强烈,均存在明显的氧漂移现象,而白石温泉则发生水气同位素交换作用,导致热水的δ^(18)O值降低。长石、方解石、石膏、黄铁矿的溶解和H_(2)S的氧化为温泉中主要离子来源。白岭温泉、汤湖温泉和白石温泉的平均热储温度分别为125、135和149℃,属于中温地热资源,其补给高程分别为1455、1790和1050 m。

关键词：中温温泉水化学特征水-岩相互作用同位素

Schisandra chinensis Oil Attenuates Aristolochic Acid Ⅰ-Induced Nephrotoxicity in vivo and in vitro

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Chinese Journal of Integrative Medicine 2022年第7期28卷 603-611页

作者： yanG yan GE Fei-lin zhan Xiao-yan MU Wen-qing LI Zhi-yong LIN Li WEI Zi-ying BAI Zhao-fang sun Qin XIAO Xiao-he School of Integrated Traditional Chinese and Western Medicine Southwest Medical UniversityLuzhouSichuan Province 646000China Senior Department of Hepatology the Fifth Medical Center of PLA General HospitalBeijing 100039China China Military Institute of Chinese Materia the Fifth Medical Center of PLA General HospitalBeijing 100039China

Objective: To investigate the protective effects of Schisandra chinensis oil(SCEO) against aristolochic acid Ⅰ(AAⅠ)-induced nephrotoxicity in vivo and in vitro and elucidate the underlying ***: C57BL/6 mice were randomly divided into 5 groups according to a random number table, including control group, AAⅠ group, and AAⅠ+SCEO(0.25, 0.5 and 1 g/kg) groups(n=5 per group). Pretreatment with SCEO was done for 2 days by oral administration, while the control and AAⅠ groups were treated with sodium carboxymethyl cellulose. Mice of all groups except for the control group were injected intraperitoneally with AAⅠ(5 mg/kg) from day 3 until day 7. Histopathological examination and apoptosis of kidney tissue were observed by hematoxylin and eosin and TdT-mediated dUTP nick-end labeling(TUNEL) staining, respectively. The levels of serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), blood urea nitrogen(BUN), and serum creatinine(SCr), as well as renal malondialdehyde(MDA), glutathione, r-glutamyl cysteingl+glycine(GSH), and superoxide dismutase(SOD) were analyzed using enzyme-linked immunosorbent assay(ELISA).Expressions of hepatic cytochrome P450 1A1(CYP1A1), CYP1A2, and nad(p)hquinonedehydrogenase1(NQO1) were analyzed using ELISA, quantitative real-time polymerase chain reaction(qPCR) and Western blot,respectively. In vitro, SCEO(40 μg/mL) was added 12 h before treatment with AAⅠ(40 μmol/mL for 48 h) in human renal proximal tubule cell line(HK-2), then apoptosis and reactive oxygen species(ROS) were analyzed by flow cytometry. Results: SCEO 0.5 and 1 g/kg ameliorated histopathological changes and TUNEL+ staining in the kidney tissues of mice with AAⅠ-induced nephrotoxicity, and reduced serum levels of ALT, AST, BUN and SCr(P<0.01 or P<0.05). SCEO 0.5 and 1 g/kg alleviated the ROS generation in kidney, containing MDA,GSH and SOD(P<0.01 or P<0.05). SCEO 1 g/kg increased the expressions of CYP1A1 and CYP1A2 and decreased NQO1 level in the liver tiss

关键词： aristolochic acidⅠ nephrotoxicity Schisandra chinensis oil metabolic enzymes apoptosis reactive oxygen species

Small Extracellular Vesicles Secreted by Peri-urethral Tissues Regulate Fibroblast Function and Contribute to the Pathogenesis of Female Stress Urinary Incontinence

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当代医学科学(英文) 2023年第4期43卷 803-810页

作者： Ning CHEN Xiao-yan sun Zhi-chen DING Jia-qi HU Wen-juan LI Li zhan Zhen-wei XIE Department of Gynecology Women's HospitalSchool of MedicineZhejiang UniversityHangzhou 310000China

Objective:This study aimed to explore the existence of small extracellular vesicles(sEVs)in peri-urethral tissues and the role of abnormal expression of sEVs in the pathogenesis of female stress urinary incontinence(SUI).Methods:sEVs were extracted from peri-urethral vaginal wall tissues using differential centrifugation and were observed by transmission electron microscopy(TEM).The number of sEVs and their protein contents were compared between SUI and control groups using nanoparticle tracking analysis(NTA)and bicinchoninic acid(BCA)protein *** were cultured separately with SUI(SsEVs group)and normal tissue sEVs(NsEVs group).Proliferation and migration of fibroblasts were compared between groups using CCK-8 and wound healing assays,*** levels of collagenⅠandⅢwere compared among blank control(BC),NsEVs,and SsEVs groups using real-time *** mass spectrometry was used to test the differentially expressed proteins contained in sEVs between ***:sEVs were extracted and found under the electron *** were significantly more sEVs extracted from the SUI group compared to the normal *** showed increased proliferative and decreased migratory abilities,and expressed more collagen in the SsEVs group compared to the NsEVs and BC *** spectrum analysis demonstrated several differentially expressed targets,including components of microfibrils,elastin polymer,and anti-inflammatory ***:sEVs were detected in the peri-urethral *** tissues expressed more sEVs than *** abnormal expression of sEVs and their protein contents may contribute to the pathogenesis and progression of SUI.

关键词： collagen female stress urinary incontinence fibroblasts small extracellular vesicles

Reference values of carotid intima-media thickness and arterial stiffness in Chinese adults based on ultrasound radio frequency signal: A nationwide, multicenter study

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中华医学杂志(英文版) 2024年第15期137卷 2024 Jul 3页

作者： Xing Changyang Xie Xiujing Wu Yu Xu Lei Guan Xiangping Li Fan zhan Xiaojun yang Hengli Li Jinsong Zhou Qi Mu Yuming Zhou Qing Ding Yunchuan Wang Yingli Wang Xiangzhu Zheng Yu sun Xiaofeng Li Hua zhang Chaoxue Zhao Cheng Qiu Shaodong yan Guozhen yang Hong Mao Yinjuan zhan Weiwei Ma Chunyan Gu Ying Chen Wu Xie Mingxing Jiang Tianan Yuan Lijun Department of Ultrasound Medicine Tangdu Hospital Air Force Medical University Xi’an Shaanxi China Department of Ultrasound Medicine The First Affiliated Hospital Zhejiang University School of Medicine Hangzhou Zhejiang China Department of Ultrasound Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei China Department of Ultrasound Xi’an Hospital of Traditional Chinese Medicine Xi’an Shaanxi China Ultrasound Medical Diagnosis Center Shaanxi Provincial People’s Hospital Xi’an Shaanxi China Department of Ultrasound Hospital of Northwestern Polytechnical University Xi’an Shaanxi China Department of Ultrasound Xi’an No. Hospital The First Affiliated Hospital of Northwest University Xi’an Shaanxi China Department of Ultrasound Diagnosis The Second Affiliated Hospital Xi’an Medical College Xi’an Shaanxi China Department of Ultrasound Xi’an Gem Flower ChangQing Hospital Xi’an Shaanxi China Department of Ultrasound The Second Affiliated Hospital of Xi’an Jiaotong University China Xi’an Shaanxi China Department of Echocardiography First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang China Department of Ultrasound Renmin Hospital of Wuhan University Wuhan Hubei China Department of Ultrasound Yan’an Hospital The Affiliated Hospital of Kunming Medical University Kunming Yunnan China Department of Ultrasound Yan’an University Xianyang Hospital Xianyang Shaanxi China Department of Function Examination Beijing University of Chinese Medicine Third Affiliated Hospital Beijing China Department of Ultrasound Xi’an Central Hospital Xi’an Shaanxi China Cadre’s Wards Ultrasound Department Diagnostic Ultrasound Center First Hospital of Jilin University Changchun Jilin China Department of Echocardiography Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University Urumqi Xinjiang China Department of Ultrasound First Affiliated Hospital of Anhui Medical University Hefei

Background: Carotid intima-media thickness (IMT) and diameter, stiffness, and wave reflections, are independent and important clinical biomarkers and risk predictors for cardiovascular diseases. The purpose of the present study was to establish nationwide reference values of carotid properties for healthy Chinese adults and to explore potential clinical ***: A total of 3053 healthy Han Chinese adults (1922 women) aged 18-79 years were enrolled at 28 collaborating tertiary centers throughout China between April 2021 and July 2022. The real-time tracking of common carotid artery walls was achieved by the radio frequency (RF) ultrasound system. The IMT, diameter, compliance coefficient, β stiffness, local pulse wave velocity (PWV), local systolic blood pressure, augmented pressure (AP), and augmentation index (AIx) were then automatically measured and reported. Data were stratified by age groups and sex. The relationships between age and carotid property parameters were analyzed by Jonckheere-Terpstra test and simple linear regressions. The major clinical determinants of carotid properties were identified by Pearson’s correlation, multiple linear regression, and analyses of ***: All the parameters of carotid properties demonstrated significantly age-related trajectories. Women showed thinner IMT, smaller carotid diameter, larger AP, and AIx than men. The β stiffness and PWV were significantly higher in men than women before forties, but the differences reversed after that. The increase rate of carotid IMT (5.5 μm/year in women and 5.8 μm/year in men) and diameter (0.03 mm/year in both men and women) were similar between men and women. For the stiffness and wave reflections, women showed significantly larger age-related variations than men as demonstrated by steeper regression slopes (allP for age by sex interaction <0.05). The blood pressures, body mass index (BMI), and triglyceride levels were identified as major clinical determinants o

关键词： Intima-media thickness Arterial stiffness Reference values Ultrasound radio frequency signal Wave reflections

Early Permian sunidyouqi suprasubduction-zone ophiolites in the central Solonker suture zone(Inner Mongolia, China)

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Geoscience Frontiers 2019年第3期10卷 1101-1111页

作者： Guo Xu Huichuan Liu Yinglei Li zhan Xu yan Xie School of Earth Sciences China University of Geosciences Inner Mongolia Key Laboratory of Magmatic Mineralization and Ore-Prospecting Geological Survey Institute of Inner Mongolia School of Earth Science and Geological Engineering Sun Yat-Sen University

Different final closing ages have been proposed for the evolution of the Paleo-Asian Ocean(PAO),including Late Silurian, pre-Late Devonian, Early Permian, Late-Permian and Late Permian-Early *** represent fragments of ancient oceanic crust and play an important role in identifying the suture zone and unveiling the evolutionary history of fossil oceans. Our detailed geological, geochemical and geochronological investigations argue for the existence of Early Permian(297 Ma) SSZ type ophiolites in the sunidyouqi area of central Inner Mongolia, China. The gabbros and basalts show LREE depleted REE patterns and left-leaning primitive mantle-normalized spider diagrams with variable negative Nb-Ta anomalies(Nb~*= 0.24-1.28 and 0.29-0.55, respectively). The sunidyouqi ophiolites were generated in a mature back-arc basin. The sunidyouqi ophiolites share the same petrological,geochemical and geochronological characteristics with the other ophiolites along the Solonker suture zone, delineating a Late Paleozoic ocean and arc-trench system. This Late Paleozoic ocean and arc-trench system coincides with a Permian paleobiogeographical boundary, i.e. the boundary between the northern cold climate(Boreal faunal-Angaraland floral realm), and a southern warm climate(Tethys faunal-Cathaysian floral realm). A tectonic scenario was proposed at last for the closure of the SE PAO involving(1) Late Ordovician to Middle Permian continuous southward subduction beneath the northern margin of North China;(2) Carboniferous to Middle Permian continuous northward subduction the forming the Northern Accretionary Orogen;(3) Late Permian final closure of the SE PAO.

关键词： Paieo-Asian Ocean Solonker North China Craton Ophiolite SSZ

四川龙门山断裂带高精度地壳/岩石圈黏度结构及其动力学意义

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地球物理学报 2020年第1期63卷 196-209页

作者：朱涛詹艳孙翔宇张宇中国地震局地球物理研究所(中国地震局地震观测与地球物理成像重点实验室) 北京100081 中国地震局地质研究所地震动力学国家重点实验室北京100029 中国地震局地壳应力研究所北京100085

岩石圈黏度是大陆动力学研究中一个重要参数,但是岩石圈黏度,尤其是横向小尺度(<100 km)黏度结构的确定是一个挑战.本文根据电阻率和黏度与它们控制因素的相似关系,直接把一条跨过青藏高原东缘和四川龙门山断裂带的大地电磁(MT)探测的... 详细信息

岩石圈黏度是大陆动力学研究中一个重要参数,但是岩石圈黏度,尤其是横向小尺度(<100 km)黏度结构的确定是一个挑战.本文根据电阻率和黏度与它们控制因素的相似关系,直接把一条跨过青藏高原东缘和四川龙门山断裂带的大地电磁(MT)探测的电阻率剖面转换成黏度结构作为输入,在GPS速度和地表地形数据的约束下,利用地球动力学数值模拟获得了该剖面的二维地壳/岩石圈黏度结构.本文推断的黏度与前人获得的区域尺度的黏度值一致,但揭示出了更多的细节.本文的黏度结构揭示出研究区域内的地壳/岩石圈黏度存在较大的空间变化范围(约5量级),黏度值分布在1.48×10^17~8.44×10^22 Pa·s之间;龙门山断裂带下的黏度存在强烈的小尺度横向变化,其中、下地壳的黏度分别为1.99×10^18~8.21×10^20 Pa·s(平均1.17×10^20 Pa·s)和4.09×10^19~7.08×10^20 Pa·s(平均1.77×10^20 Pa·s).基于该黏度结构的地球动力学模型表明驱动青藏高原中-下地壳物质流动的可能是热-化学浮力,以及上地壳和中-下地壳可能处于解耦状态.本文获得的黏度结构可以为龙门山断裂带地震成因和机制、岩石圈小尺度变形和构造应力状态的深入研究提供重要的帮助.

关键词：黏度结构大地电磁测深电阻率地球动力学模拟

Neat1 decreases neuronal apoptosis after oxygen and glucose deprivation

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Neural Regeneration Research 2022年第1期17卷 163-169页

作者： Wei-Na Chai Yi-Fan Wu Zhi-Min Wu yan-Feng Xie Quan-Hong Shi Wei Dan yan zhan Jian-Jun Zhong Wei Tang Xiao-Chuan sun Li Jiang Department of Neurosurgery First Affiliated Hospital of Chongqing Medical UniversityChongqingChina

Studies have shown that downregulation of nuclear-enriched autosomal transcript 1(Neat1)may adversely affect the recovery of nerve function and the increased loss of hippocampal neurons in *** Neat1 has protective or inhibitory effects on neuronal cell apoptosis after secondary brain injury remains ***,the effects of Neat1 on neuronal apoptosis were observed.C57 BL/6 primary neurons were obtained from the cortices of newborn mice and cultured in vitro,and an oxygen and glucose deprivation cell model was established to simulate the secondary brain injury that occurs after traumatic brain injury in *** level of Neat1 expression in neuronal cells was regulated by constructing a recombinant adenovirus to infect neurons,and the effects of Neat1 expression on neuronal apoptosis after oxygen and glucose deprivation were *** experiment was divided into four groups:the control group,without any treatment,received normal culture;the oxygen and glucose deprivation group were subjected to the oxygen and glucose deprivation model protocol;the Neat1 overexpression and Neat1 downregulation groups were treated with Neat1 expression intervention techniques and were subjected to the in oxygen and glucose deprivation *** protein expression levels of neurons p53-induced death domain protein 1(PIDD1,a pro-apoptotic protein),caspase-2(an apoptotic priming protein),cytochrome C(a pro-apoptotic protein),and cleaved caspase-3(an apoptotic executive protein)were measured in each group using the western blot *** observe changes in the intracellular distribution of cytochrome C,the expression levels of cytochrome C in the cytoplasm and mitochondria of neurons from each group were detected by western blot *** in the cell viability and apoptosis rate between groups were detected by cell-counting kit 8 assay and terminal deoxynucleotidyl transferase dUTP nick-end labeling assay,*** results showed that the apoptosis rat

关键词： apoptosis caspase-2 caspase-3 cytochrome C LncRNA-Neat1 mitochondria neuron OGD PIDD1 traumatic brain injury

Urinary metabolomics analysis identifies key biomarkers of different stages of nonalcoholic fatty liver disease

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World Journal of Gastroenterology 2017年第15期23卷 2771-2784页

作者： Shu Dong Zong-Ying zhan Hong-yan Cao Chao Wu yan-qin Bian Jian-Yuan Li Gen-Hong Cheng Ping Liu Ming-Yu sun Key Laboratory of Liver and Kidney Diseases Institute of Liver Diseases Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine Department of Microbiology Immunology and Molecular Genetics David Geffen School of Medicine University of California

AIM To identify a panel of biomarkers that can distinguish between non-alcoholic fatty liver disease(NAFLD) and non-alcoholic steatohepatitis(NASH), and explore molecular mechanism involved in the process of developing NASH from *** Biomarkers may differ during stages of NAFLD. Urine and blood were obtained from non-diabetic subjects with NAFLD and steatosis, with normal liver function(n = 33), from patients with NASH, with abnormal liver function(n = 45), and from healthy age and sex-matched controls(n = 30). Samples were subjected to metabolomic analysis to identify potential non-invasive biomarkers. Differences in urinary metabolic profiles were analyzed using liquid chromatography tandem mass spectrometry with principal component analysis and partial least squares-discriminate *** Compared with NAFLD patients, patients with NASH had abnormal liver function and high serum lipid concentrations. Urinary metabonomics found differences in 31 metabolites between these two groups, including differences in nucleic acids and amino acids. Pathway analysis based on overlapping metabolites showed that pathways of energy and amino acid metabolism, as well as the pentose phosphate pathway, were closely associated with pathological processes in NAFLD and *** These findings suggested that a panel of biomarkers could distinguish between NAFLD and NASH, and could help to determine the molecular mechanism involved in the process of developing NASH from NAFLD. Urinary biomarkers may be diagnostic in these patients and could be used to assess responses to therapeutic interventions.

关键词： Urinary metabonomics Nonalcoholic fatty liver disease Steatohepatitis