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新型冠状病毒肺炎早期时空传播特征分析

物理学报 2020年第8期69卷 120-129页

作者：王聪严洁王旭李敏四川警察学院计算机科学与技术系泸州646000 四川警察学院四川警事科学研究院成都610200 四川警察学院道路交通管理系泸州646000 四川师范大学影视与传媒学院成都610068 四川师范大学计算机科学学院成都610068

通过最新公布的流行病学数据估计了易感者-感染者模型参数,结合百度迁徙数据和公开新闻报道,刻画了疫情前期武汉市人口流动特征,并代入提出的支持人口流动特征的时域差分方程模型进行动力学模拟,得到一些推论:1)未受干预时传染率在一般... 详细信息

通过最新公布的流行病学数据估计了易感者-感染者模型参数,结合百度迁徙数据和公开新闻报道,刻画了疫情前期武汉市人口流动特征,并代入提出的支持人口流动特征的时域差分方程模型进行动力学模拟,得到一些推论:1)未受干预时传染率在一般环境下以95%的置信度位于区间[0.2068,0.2073],拟合优度达到0.999;对应地,基本传染数R0位于区间[2.5510,2.6555];极限环境个案推演的传染率极值为0.2862,相应的R0极值为3.1465;2)百度迁徙规模指数与铁路发送旅客人数的Pearson相关系数达到0.9108,有理由作为人口流动的有效估计;3)提出的模型可有效推演疫情蔓延至外省乃至全国的日期,其中41.38%的预测误差≤1 d,79.31%的预测误差≤3 d,96.55%预测误差≤5 d,总体平均误差约为2.14 d.

关键词：新型冠状病毒肺炎流行病学模型交通流人口迁徙

远端缺血适应外泌体对大鼠心脏骤停后脑损伤的保护作用

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中华急诊医学杂志 2024年第7期33卷 991-998页

作者：翟恒王吉慧陈习张扬胡书群王军彭易根赵宁军燕宪亮苏成磊许铁徐州医科大学附属医院急诊医学科徐州221006 睢宁县人民医院急诊医学科睢宁221200 徐州医科大学第二临床医学院急诊医学实验室徐州221006 南京大学医学院附属鼓楼医院急诊医学科南京210008 南京市江宁医院急诊医学科南京211100 徐州医科大学江苏省卫生应急研究所徐州221006

目的探讨远端缺血适应来源的外泌体对大鼠心脏骤停后神经功能障碍的治疗作用。方法Sprague-Dawley大鼠在经历远端缺血适应后,从血浆中分离出外泌体用作干预药物。36只大鼠被随机分为假手术组、对照组和外泌体组(n=12)。对照组和外泌体... 详细信息

目的探讨远端缺血适应来源的外泌体对大鼠心脏骤停后神经功能障碍的治疗作用。方法Sprague-Dawley大鼠在经历远端缺血适应后,从血浆中分离出外泌体用作干预药物。36只大鼠被随机分为假手术组、对照组和外泌体组(n=12)。对照组和外泌体组大鼠使用窒息法诱导心脏骤停,恢复自主循环后分别静脉输注安慰剂或外泌体悬液(1×1010 Particles)。神经功能缺陷评分、旷场试验、Y迷宫和水迷宫用以评估神经功能结果。检测血浆透明质酸(hyaluronic acid,HA)和多配体蛋白聚糖-1(syndecan-1,Sdc-1)水平、检测海马CA1区基质金属蛋白酶2/9(matrix metalloproteinase-2/9,MMP-2/9)的表达水平。结果在经历远端缺血适应后,大鼠血浆中外泌体含量较正常大鼠升高。和对照组相比,外泌体组大鼠行为学实验明显改善,表现为水平运动距离增加(5.86±2.89 vs.17.53±5.51,P<0.05)、自发交替正确率升高(13.29±15.07 vs.42.63±10.25,P<0.05)、逃避潜伏期缩短(25.83±8.54 vs.13.49±4.55,P<0.05)。复苏后24 h的血浆HA和Sdc-1水平明显降低(HA:26.34±9.83 vs.14.84±6.26,P<0.05;Sdc-1:0.05±0.03 vs.0.02±0.02,P<0.05),同时海马组织中MMP-2/9水平明显降低。结论从经历远端缺血适应的大鼠血浆中提取出的外泌体能够改善大鼠心脏骤停后神经功能障碍,其机制可能与MMP-2/9的减少以内皮糖萼降解减少有关。

关键词：远端缺血适应外泌体心脏骤停神经保护糖萼透明质酸多配体蛋白聚糖-1 基质金属蛋白酶2 基质金属蛋白酶9

不同剂量环硅酸锆钠快速纠正肾移植等待人群围手术期高钾血症的疗效和安全性

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中华泌尿外科杂志 2024年第2期45卷 127-131页

作者：方翊灵罗佳夏仁飞严紫嫣王於尘曾文利邓文锋徐健苗芸南方医科大学南方医院器官移植科广州510515

目的探讨不同剂量环硅酸锆钠(SZC)快速纠正不同血钾水平的肾移植等待人群围手术期高钾血症的疗效和安全性。方法采用前瞻性研究,选取2023年6月至2024年1月于南方医科大学南方医院服用SZC治疗高钾血症(随机静脉血钾>5.0mmol/L)的患者。... 详细信息

目的探讨不同剂量环硅酸锆钠(SZC)快速纠正不同血钾水平的肾移植等待人群围手术期高钾血症的疗效和安全性。方法采用前瞻性研究,选取2023年6月至2024年1月于南方医科大学南方医院服用SZC治疗高钾血症(随机静脉血钾>5.0mmol/L)的患者。纳入标准:①诊断为慢性肾脏病(CKD)5期的首次肾移植术等待患者;②年龄18~65岁;③血清钾5.0~7.0mmol/L排除标准:①联合其他降钾治疗者;②妊娠或哺乳期妇女;③经研究者判断认为不适合参与本试验的患者。采用随机数字表法,随机予3种剂量(5g、10g、15g)SZC,分别于服药后0.5、1、2、3、4h检测静脉血钾,记录服药后4h内血钾降低幅度和恢复正常例数,观察不良反应发生情况。根据基线血钾水平将患者分为轻度高钾组(血钾5.0~5.5mmol/L)和中重度高钾组(血钾≥5.5mmol/L),记录两组服药后4h内血钾降低幅度和恢复正常例数。分析服药剂量、基线血钾水平与服药后4h血钾恢复情况的相关性。记录患者是否出现水肿、胃肠道症状等,评估药物安全性。结果本研究纳入34例患者,5g组10例,10g组13例,15g组11例,3组的年龄[(41.90±11.51)岁与(44.15±10.51)岁与(42.91±10.78)岁]、性别比(男/女:9/1例与9/4例与8/3例)、体质量指数[(23.08±3.54)kg/m^(2)与(23.03±2.92)kg/m^(2)与(23.47±2.99)kg/m^(2)]、估算肾小球滤过率[(4.80±2.13)ml/(min·1.73m^(2))与(5.26±1.14)ml/(min·1.73m^(2))与(4.85±1.48)ml/(min·1.73m^(2))、基线血钾[(5.65±0.43)mmol/L与(5.59±0.25)mmol/L与(5.94±0.64)mmol/L]差异均无统计学意义(P>0.05)。服药后4h,5、10、15g组的血钾水平分别为(5.20±0.65)、(5.03±0.35)、(5.10±0.46)mmol/L,与基线相比差异均有统计学意义(Pmin1.73m^(2))与(5.21±1.68)ml/(min·1.73m^(2))]和服药剂量(5g/10g/15g:4/4/4例与6/9/7例)差异无统计学意义(P>0.05)。服药后4h,轻度高钾组和中重度高钾组分别有9例(75.0%)和4例(18.2%)血钾恢复正常,差异有统计学意义(χ^(2)=10.614,P=0.002)。服药后4h血钾是否恢复正常与基线血钾显著相关(r=0.483,P=0.001),与服药剂量无显著相关性(r=0.118,P=0.474)。所有患者服药后4h内均未发生低钾血症、水肿、恶心、呕吐等不良反应。结论SZC用于并发轻度高钾血症的肾移植围手术期患者安全、有效,可达到4h内快速降低血钾水平的目的。

关键词：高钾血症慢性肾脏病5期环硅酸锆钠肾移植围手术期

Prim-O-glucosylcimifugin ameliorates aging-impaired endogenous tendon regeneration by rejuvenating senescent tendon stem/progenitor cells

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Bone Research 2023年第4期11卷 784-802页

作者： Yu Wang Shanshan Jin Dan Luo Danqing He min Yu Lisha Zhu Zixin Li Liyuan Chen Chengye Ding Xiaolan Wu Tianhao Wu Weiran Huang xuelin Zhao Meng xu Zhengwei Xie yan Liu Laboratory of Biomimetic Nanomaterials Department of OrthodonticsPeking University School and Hospital of Stomatology&National Center for Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental Materials&Translational Research Center for Orocraniofacial Stem Cells and Systemic HealthBeijing 100081China CAS Center for Excellence in Nanoscience Beijing Key Laboratory of Micro-nano Energy and SensorBeijing Institute of Nanoenergy and NanosystemsChinese Academy of SciencesBeijing 101400China Peking university International Cancer Institute Health Science CenterPeking UniversityBeijing 100083China department of Orthopedics the Fourth Medical Center of PLA General HospitalBeijing 100048China

Adult tendon stem/progenitor cells(TSPCs)are essential for tendon maintenance,regeneration,and repair,yet they become susceptible to senescence with age,impairing the self-healing capacity of *** this study,we employ a recently developed deep-learning-based efficacy prediction system to screen potential stemness-promoting and senescence-inhibiting drugs from natural products using the transcriptional signatures of *** top-ranked candidate,prim-O-glucosylcimifugin(POG),a saposhnikovia root extract,could ameliorate TPSC senescent phenotypes caused by long-term passage and natural aging in rats and humans,as well as restore the self-renewal and proliferative capacities and tenogenic potential of aged *** vivo,the systematic administration of POG or the local delivery of POG nanoparticles functionally rescued endogenous tendon regeneration and repair in aged rats to levels similar to those of normal ***,POG protects TSPCs against functional impairment during both passage-induced and natural aging by simultaneously suppressing nuclear factor-κB and decreasing mTOR signaling with the induction of ***,the strategy of pharmacological intervention with the deep learning-predicted compound POG could rejuvenate aged TSPCs and improve the regenerative capacity of aged tendons.

关键词： tendon endogenous progenitor

Functional Cure of Chronic Hepatitis B with Antiviral Treatment in Children having High-level Viremia and normal or Mildly Elevated Serum Aminotransferase

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Journal of Clinical and Translational Hepatology 2023年第5期11卷 1011-1022页

作者： Jing Li Peiyao Fan Zhiqiang xu Yi Dong Fuchuan Wang Weiguo Hong Jinfang Zhao Yinjie Gao Jianguo yan Lili Cao Chao Zhang Shishu Zhu Fu-Sheng Wang min Zhang Peking university 302 Clinical Medical School BeijingChina Senior department of Infectious Diseases The Fifth Medical Center of Chinese PLA General HospitalNational Clinical Research Center for Infectious DiseasesBeijingChina Senior department of Liver Diseases The Fifth Medical Center of Chinese PLA General HospitalBeijingChina

Background and Aims:There is a lack of data supporting the notion that antiviral treatments can beneft children with chronic hepatitis B(CHB)having high viremia and normal or mildly elevated serum alanine aminotransferase(ALT)*** aimed t analyze the efficacy of antiviral treatments in children with CHB and explore the factors associated with functional ***:Forty-eight children with CHB having high viremia and normal or mildly elevated serum ALT levels were screened in this real-world ***-two children received either interferon-alpha(IFN-α)monother-apy,IFN-a therapy with a nucleoside analog(NA)add-on,or IFN-α and NA combination *** 16 children in the control group did not receive antiviral *** 48 chil-dren were available for follow-up assessments for the entire 36-month study *** identified a functional cure with respect to hepatitis B virus(HBV)DNA loss,loss/seroconver-sion of circulating hepatitis B e antigen(HBeAg),and loss of hepatitis B surface antigen(HBsAg)with or without *** regression analysis was employed to evaluate the factors that may have influenced the functional ***-sults:After 36 months,the cumulative functional cure rate was 56.25%(18/32)in the treated group and 0%(0/16)in the control group(prum HBV DNA levels declined rapidly at the end of a 6-month visit and the cured children achieved a loss rate of 100%(18/18)within 16 months of beginning treatment,compared with 64.29%(9/14)of the uncured children(prum HBV DNA and were positive for both serum HBeAg and HBsAg during the entire 36 months of the study *** tional cure was associated with younger ages(1-6 vs.7-14 years,p=0.013),CD8^(+)T lymphocyte counts(p=0.013),and B lymphocyte counts(p=0.003).

关键词： Child HBV Chronic hepatitis B Antiviral therapy Functional cure

α-fetoprotein involvement during glucocorticoid-induced precocious maturation in rat colon

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World Journal of Gastroenterology 2011年第24期17卷 2933-2940页

作者： min Chen Peng Sun Xiao-yan Liu Dan Dong Jun Du Luo Gu Ying-Bin Ge Lian Yun Gang Higher Vocational Technical College of Traditional Chinese Medicine Lianyungang 222006 Jiangsu Province China department of Physiology Nanjing Medical University Nanjing 210029 Jiangsu Province China The Laboratory Center for Basic Medical Sciences Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM： TO investigate the role of a-fetoprotein （AFP）, a cancer-associated fetal glycoprotein, in glucocorticoidinduced precocious maturation in rat colon. METHODS： Colons from suckling Sprague-Dawley rats were used in this study. Corticosterone acetate at a dose of 100 μg/g body weight was given to normal pups on days 7, 9 and 11 after birth to induce hypercorticoidism. Control animals were injected with identical volumes of normal saline. Some rats receiving corticosterone 7 d after birth were also treated with mifepristone （ru38486）, a glucocorticoid cytoplasm receptor antagonist to investigate the effects of glucocorticoids （GCs）. The morphological changes of the crypt depth and villous height of the villous zone in colon were observed as indicesof colon maturation. Expression levels of AFP in colons were detected by reverse transcriptase polymerase chain reaction and Western blotting. To identify the cellular lo- calization of AFP in developing rat colons, double-immu- nofluorescent staining was performed using antibodies to specific mesenchymal cell marker and AFP. RESULTS： Corticosterone increased the crypt depth and villous height in the colon of 8- and 10-d-old rats with hypercorticoidism compared with that in the control ani- mals （120% in 8-d-old rats and 118% in 10-d-old rats in villous height, P = 0.021; 145% in 8-d-old rats and 124% in 10-d-old rats in crypt depth, P = 0.017）. These increases were accompanied by an increase of AFP ex- pression in both mRNA and protein （2.5-folds in 8-d- old and 2.5-folds in 10-d-old rats higher than in control animals, P = 0.035; 1.8-folds in 8-d-old and 1.3-folds in 10-d-old rats higher than in control animals, P = 0.023）. Increased crypt depth and villous height and increased expression of AFP in the colon of rats with hypercorti- coidism were blocked by mifepristone. Both had positive staining for AFP or vimentin, and overlapped in mesen- chymal cells at each tested colon. CONCLUSION： GCs promote the development of ra

关键词： Glucocorticoids a-fetoprotein Precociousmaturation Colon Rat

Guidelines for the diagnosis and treatment of chronic pancreatitis in china(2018 edition)

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Hepatobiliary & Pancreatic Diseases International 2019年第2期18卷 103-109页

作者： Wen-Bin Zou Nan ru Hao Wu Liang-Hao Hu xu Ren Gang Jin Zheng Wang Yi-Qi Du Ya-Nan Cao Lei Zhang Xiao-yan Chang Rong-Chun Zhang Xiao-Bin Li yan Shen Peng Li Zhao-Shen Li Zhuan Liao Pancreatitis Group of Chinese Medical Doctor Association department of Gastroenterology Digestive Endoscopy CenterChanghai HospitalNaval Medical UniversityShanghai 200433China department of General Surgery Peking Union Medical College HospitalBeijing 100730China department of Hepatobiliary Pancreatic Surgery First Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310003China department of Digestive Endoscopy Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China 不详 Shanghai Institute of Pancreatic Diseases Shanghai 200433China department of Gastroenterology Heilongjiang Provincial HospitalHarbin 150030China department of Hepatobiliary Pancreatic Surgery Changhai HospitalNaval Medical UniversityShanghai 200433China department of Hepatobiliary Surgery First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061China Shanghai Clinical Center for Endocrine and Metabolic Diseases Ruijin HospitalSchool of MedicineShanghai Jiaotong UniversityShanghai 200025China department of Radiology Shanghai First People’s HospitalShanghai 200080China department of Pathology Peking Union Medical College HospitalBeijing 100730China department of Gastroenterology Xijing HospitalFourth Military Medical UniversityXi’an 710032China

Chronic pancreatitis(CP)is a progressive inflammatory disease of pancreas that alters the organ’s normal structure and *** seriously affects the quality of life of patients and greatly increases the public medical burden of *** recent years,the incidence of CP has increased *** concepts of etiology and therapies have been continuously updated.

关键词： Guidelines diagnosis and treatment pancreatitis

A Bayesian Stepwise Discriminant Model for Predicting Risk Factors of Preterm Premature rupture of Membranes： A Case-control Study

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Chinese Medical Journal 2017年第20期130卷 2416-2422页

作者： Li-Xia Zhang yang Sun Hai Zhao Na Zhu Xing-De Sun Xing Jin Ai-min Zou yang Mi Ji-ru xu department of Microbiology and Immunology Health Science Center Xi'an Jiaotong University Xi'an Shaanxi 710061 China Deparment of Clinical Laboratory Shaanxi Provincial People's Hospital Xi'an Shaanxi 710068 China department of Medical Statistics Shaanxi Provincial People's Hospital Xi'an Shaanxi 710068 China Deparment of Clinical Laboratory Xi'an Fourth Hospital Xi'an Shaanxi 710004 China Deparment of Clinical Laboratory Xi'an Gaoxin Hospital Xi'an Shaanxi 710075 China Deparment of Clinical Laboratory Chang'an Hospital Xi'an Shaanxi 710018 China department of Obstetrics and Gynecology The Northwest Women and Children Hospital Xi'an Shaanxi 710061 China

Background： Preterm premature rapture of membrane （PPROM） can lead to serious consequences such as intrauterine infection, prolapse of the umbilical cord, and neonatal respiratory distress syndrome. Genital infection is a very important risk which closely related with PPROM. The preliminary study only made qualitative research on genital infection, but there was no deep and clear judgment about the effects of pathogenic bacteria. This study was to analyze the association of in fections with PPROM in pregnant women in Shaanxi, china, and to establish Bayesian stepwise discriminant analysis to predict the incidence of PPROM. Methods： In training group, the 112 pregnant women with PPROM were enrolled in the case subgroup, and 108 normal pregnant women in the control subgroup using an unmatched case-control method. The sociodemographic characteristics of these participants were collected by face-to-face interviews. Vaginal excretions fiom each participant were sampled at 28 36-6 weeks of pregnancy using a sterile swab. DNA corresponding to Chlamrdia trachomalix （CT）, Ureaplasma urealyticwn （UU）, Candida albicans, group B streptococci （GBS）, herpes simplex virus- 1 （HSV-1）, and HSV-2 were detected in each participant by real-time polymerase chain reaction. A model of Bayesian discriminant analysis was established and then verified by a mull）center validation group that included 500 participants in the case subgroup and 5（10 participants in the control subgroup from five different hospitals in the Shaanxi province, respectively. Results： The sociological characteristics were not significantly different between the case and control subgroups in both training and validation groups （all P 〉 0.05）. In training group, the infection rates of UU （11.6% vs. 3.7%）, CT （17.0% vs. 5.6%）, and GBS （22.3% vs. 6.5%） showed statistically different between the case and control subgroups （all P 〈 0.05）, Iog-transfomacd quantification of UU, CE GBS, and HSV-2 showed statistically different

关键词： Bayesian Stepwise Discfiminant Analysis EtiologicalFactors Infection Preterm Premature rupture of Membranes

Proteolipidprotein 1 gene mutation in nine patients with Pelizaeus-Merzbacher disease

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Chinese Medical Journal 2008年第17期121卷 1638-1642页

作者： WANG Jing-min WU Ye WANG Hui-fang DENG yan-hua yanG yan-ling QIN Jiong LI Xin-yi WU Xi-ru JIANG Yu-wu department of Pediatrics Peking University First Hospital Beijing 100034 China department of Neurology First Hospital Affiliated to Shanxi Medical University Taiyuan Shanxi 030001 China

Background Pelizaeus-Merzbacher disease （PMD） is a rare X-linked recessive disorder with symptoms including nystagmus, impaired motor development, ataxia, and progressive spasticity. The proteolipidprotein 1 （PLP1） gene is the only pathogenic gene of PMD. Duplication of the PLP1 gene is the most frequent gene defect, accounting for 50%-70% of PMD cases, whereas point mutations in the coding sequence or the splice sites account for 10%-25% of PMD cases. This study aimed to identify PLP1 mutations in nine unrelated Chinese patients （P1-9） with PMD, and 14 subjects from the family of patient 2 were also described. Methods Genomic DNA was extracted from peripheral multiplex ligation-dependent probe amplification （MLPA）. AI amplified and analyzed using direct DNA sequencing. blood samples. Gene dosage was determined using the 7 exons and exon-intron boundaries of the PLP1 gene were Results Of these nine patients, there were four transitional, four classical, and one connatal PMD according to their clinical and radiological presentations. PLP1 duplications were identified in patients 1-7 with PMD. Their mothers were PLPI duplications carriers as well. Both duplication carriers and normal genotypes of PLP1 were identified in the family members of patient 2. A c.517C〉T （p. P173S） hemizygous missense mutation in exon 4 was found in patient 8 with PMD, and his mother was shown to be a heterozygote of this mutation. Conclusions We identified seven genomic duplications and one missense mutation （p. P173S） of the PLP1 gene in eight Chinese patients with PMD. This is the report about PLP1 mutations in PMD patients from the mainland of china.

关键词： Pelizaeus-Merzbacher disease proteolipid protein 1 gene multiplex ligation-dependent probeamplification mutation