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HER2-Specific T Lymphocytes Kill both Trastuzumab-Resistant and Trastuzumab-Sensitive Breast Cell Lines In Vitro

Chinese Journal of Cancer Research 2012年第2期24卷 143-150页

作者： Xiao-lin Lin Xiao-li Wang BO Ma Jun Jia Ying yan Li-jun Di yan-hua yuan Feng-ling Wan yuan-li Lu Xu Liang Tao Shen Jun Ren Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education) Department of Breast CancerPeking University Cancer Hospital&Institute Beijing 100142China Key Laboratory of Geriatrics Beijing Hospital&Beijing Institute of GeriatricsMinistry of HealthBeijing 100730China

Objective： Although the development of trastuzumab has improved the outlook for women with human epidermal growth factor receptor 2 （HER2）-positive breast cancer, the resistance to anti-HER2 therapy is a growing clinical dilemma. We aim to determine whether HER2-specific T cells generated from dendritic cells （DCs） modified with HER2 gene could effectively kill the HER2-positive breast cancer cells, especially the trastuzumab-resistant cells. Methods： The peripheral blood mononuclear cells （PBMCs） from healthy donors, whose HLA haplotypes were compatible with the tumor cell lines, were transfected with reconstructive human adeno-association virus （rhAAV/HER2） to obtain the specific killing activities of T cells, and were evaluated by lactate dehydrogenase （LDH） releasing assay. Results： Trastuzumab produced a significant inhibiting effect on SK-BR-3, the IC50 was 100ng/ml. MDA-MB-453 was resistant to trastuzumab even at a concentration of 10,000 ng/ml in vitro. HER2-specific T lymphocytes killed effectively SK-BR-3 [（69.86±13.41）%] and MDA-MB-453 [（78.36±10.68）%] at 40：1 （effector：target ratio, E：T）, but had no significant cytotoxicity against HER2-negative breast cancer cell lines MDA-MB-231 or MCF-7 （less than 10%）. Conclusion： The study showed that HER2-specific T lymphocytes generated from DCs modified by rhAAV/HER2 could kill HER2-positive breast cancer cell lines in a HER2-dependent manner, and result in significantly high inhibition rates on the intrinsic trastuzumab-resistant cell line MDA-MB-453 and the tastuzumab-sensitive cell line SK-BR-3. These results imply that this immunotherapy might be a potential treatment to HER2-positive breast cancer.

关键词： HER2-positive breast cancer Trastuzumab-resistant Dendritic cells Immunotherapy Reconstructivehuman adeno-association virus

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Magnetic and Transport Properties of Bi_(0.5)Ca_(0.5–x)La_xFe_(0.3)Mn_(0.7)O_3(x=0, 0.05, 0.10, 0.15, 0.20, 0.25) Prepared by Molten Salt Method

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Chemical Research in Chinese Universities 2011年第1期27卷 15-18页

作者： DONG Zhong-ping HU Bin CHEN yan yuan Hong-ming FENG Shou-hua State Key Laboratory of Inorganic Synthesis ＆ Preparative Chemistry College of Chemistry Jilin University Changchun 130012 P. R. China

Polycrystallines Bi0.5Ca0.5-xLaxFe0.3Mn0.7O3（x=0, 0,05, 0.10, 0.15, 0.20, 0.25） were prepared by molten salt method and showed perovskite orthorhombic structure with space group Pnma. The magnetic measurements indicate that the compounds exhibit antiferromagnetic behavior in a temperature range of 4-300 K. The measurements of transport properties suggest that the substitution of La for Ca enhanced the conductivity, and a kink appeared on the curve of temperature dependence of resistivity at 275 K, which is related to the spin-reorientation in the samples.

关键词： Perovskite Doping Magnetism Spin-reorientation

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Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical Neurons Exposed to Cadmium in Vitro

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Biomedical and Environmental Sciences 2012年第2期25卷 172-181页

作者： yuan yan BIAN Jian Chun LIU Xue Zhong ZHANG Ying SUN Ya LIU Zong Ping College of Veterinary Medicine Yangzhou UniversityYangzhou 225009JiangsuChina

Objective To investigate the cytotoxic mechanism of cadmium（Cd） on cerebral cortical *** The primary cultures of rat cerebral cortical neurons were treated with different concentrations of cadmium acetate（0,5,10,and 20 μmol/L）,and then the cell viability,apoptosis,ultrastructure,intracellular [Ca2＋]i and reactive oxygen species（ROS） levels,mitochondrial membrane potential（ΔΨ）,activities of catalase（CAT） and superoxide dismutase（SOD） were *** A progressive loss in cell viability and an increased number of apoptotic cells were *** addition,Cd-induced apoptotic morphological changes in cerebral cortical neurons were also demonstrated by Hoechst 33258 ***,ultrastructural changes were distortion of mitochondrial cristae and an unusual ***,elevation of intracellular [Ca2＋]i and ROS levels,depletion of ΔΨ were revealed in a dose-dependent manner during the ***,CAT and SOD activities in the living cells increased *** Exposure of cortical neurons to different doses of Cd led to cellular death,mediated by an apoptotic mechanism,and the apoptotic death induced by oxidative stress may be a potential *** the disorder of intracellular homeostasis caused by oxidative stress and mitochondrial dysfunction may be a trigger for apoptosis in cortical neurons.

关键词： Cadmium Apoptosis Oxidative stress Cerebral cortical neurons

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Performance evaluation of the effect of humic acid on Anammox granular sludge:Apparent morphology,nitrogen removal and microbial community

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Journal of Environmental Sciences 2024年第10期144卷 148-158页

作者： Wenhui Huang Peiling Xu Xiang Li Yong Huang Hao Sun Wei Li Mao Zhang Miao Shi yan yuan School of Environmental Science and Engineering Suzhou University of Science and TechnologySuzhou 215009China Suzhou Hongyu Environmental Technology Company Limited by Shares Suzhou 215011China Suzhou Tianjun Environmental Technology Limited Company Suzhou 215011China Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment Suzhou University of Science and TechnologySuzhou 215009China

Humic acid(HA)is a typical refractory organicmatter,so it is of great significance to investigate its effect on the performance of Anammox granular *** the dosage ofHA≤50 mg/L,HA promotes the total nitrogen removal rate(NRR)to 1.45 kg/(m^(3)·day).When HA was between 50 and 100 mg/L,the NRR of Anammox was *** this time,the adsorption of HA causes the sludge to gradually turn fromred to brown,but the activities of heme and enzymes showed that its capacitywas not *** HA levels reached 250 mg/L,the NRR dropped to 0.11 kg/(m^(3)·day).Moderate HA levels promoted the release of extracellular polymeric substance(EPS),but excessive HA levels lead to a decrease in EPS *** inhibited Anammox activity,which indirectly hindered the transmission of substrate and accumulated substrate *** HA promoted the increase of heterotrophicmicrobial abundance in Anammox system,the microbial diversity decreased *** the increase of HA concentration,the abundance of Candidatus_Brocadia,the main functional microorganism of Anammox system,decreased gradually,while the abundance of Candidatus_Kuenenia increased gradually.

关键词： Anammox Landfill leachate Humic acid Heme Microbial community Enzyme activity

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Gene polymorphisms associated with sudden decreases in heart rate during extensive peritoneal lavage with distilled water after gastrectomy

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World Journal of Gastrointestinal Surgery 2023年第10期15卷 2154-2170页

作者： Shuang Yao yan yuan Jun Zhang yang Yu Guang-Hua Luo Clinical Medical Research Center The Third Affiliated Hospital of Soochow UniversityChangzhou 213000Jiangsu ProvinceChina Department of Anesthesiology The Third Affiliated Hospital of Soochow UniversityChangzhou 213000Jiangsu ProvinceChina

BACKGROUND Our previous study found that the telomerase-associated protein 1(TEP1,rs938886 and rs1713449)and homo sapiens RecQ like helicase 5(RECQL5,rs820196)single nucleotide polymorphisms(SNPs)were associated with changes in heart rate(HR)≥30%during peritoneal lavage with distilled water after *** study established a single tube method for detecting these three SNPs using two-dimensional(2D)polymerase chain reaction(PCR),and investigated whether SNP-SNP and SNP-environment interactions increase the risk of high HR variability(HRV).AIM To investigate whether genotypes,genetic patterns,SNP-SNP and SNP-environment interactions were associated with *** 2D PCR was used to establish a single-tube method to detect TEP1 rs938886 and rs1713449 and RECQL5 rs820196,and the results were compared with those of sanger *** adjusting for confounders such as age,sex,smoking,hypertension,and thyroid dysfunction,a nonconditional logistic regression model was used to assess the associations between the genotypes and the genetic patterns(codominant,dominant,overdominant,recessive,and additive)of the three SNPs and a risk≥15%or≥30%of a sudden drop in HR during postoperative peritoneal lavage in patients with gastric ***-gene and geneenvironment interactions were analyzed using generalized multifactor dimensionality *** The coincidence rate between the 2D PCR and sequencing was 100%.When the HRV cutoff value was 15%,the patients with the RECQL5(rs820196)TC genotype had a higher risk of high HRV than those who had the TT genotype(odds ratio=1.97;95%CI:1.05-3.70;P=0.045).Under the codominant and overdominant models,the TC genotype of RECQL5(rs820196)was associated with a higher risk of HR decrease relative to the TT and TT+CC genotypes(P=0.031 and 0.016,respectively).When the HRV cutoff value was 30%,patients carrying the GC-TC genotypes of rs938886 and rs820196 showed a higher HRV risk when compared with the GG–TT genotype carriers

关键词： Homo sapiens RecQ like helicase 5 Telomerase-associated protein 1 Polymorphism Peritoneal lavage Heart rate variability Two-dimensional polymerase chain reaction

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Performance Diagnosis of Controller Based on Eigenvector Subspace K-mean Clustering 36

Performance Diagnosis of Controller Based on Eigenvector Sub...

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第36届中国控制会议

作者： Man Hao Wei-Hua Cao Min Wu yan yuan Zhen-Tao Liu School of Automation China University of Geosciences Hubei key Laboratory of Advanced Control and Intelligent Automation for Complex Systems

ISBN: (纸本)9781538629185

To diagnose the specific reasons that lead to deterioration of controller performance and improve the diagnostic accuracy,a performance diagnosis method based on eigenvector subspace K-mean clustering is ***,the number of standard deterioration performance subspace with different degrees of deterioration information is increased on the basis of the eigenvector subspace distance diagnosis method,in which multiple distance values between eigenvector subspaces are ***,K-mean clustering is used to divide these distance values into two classes,and the clustering center with a maximum number of points is selected as the representative ***,current performance degradation factor is attributed to the category that represented by the nearest standard performance *** simulation,a double-tank model is established,and simulation result shows that average accuracy of controller performance diagnosis reaches 99.4%approximately.

关键词： controller performance performance diagnosis eigenvector subspace K-mean clustering

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X-ray detection based on complementary metal-oxide-semiconductor sensors

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Nuclear Science and Techniques 2019年第1期30卷 43-48页

作者： Qian-Qian Cheng Chun-Wang Ma yan-Zhong yuan Fang Wang Fu Jin Xian-Feng Liu College of Physics and Materials Science Henan Normal University College of Electronic and Electrical Engineering Henan Normal University Chongqing Cancer Hospital

Complementary metal-oxide-semiconductor(CMOS) sensors can convert X-rays into detectable signals; therefore, they are powerful tools in X-ray detection applications. Herein, we explore the physics behind X-ray detection performed using CMOS sensors. X-ray measurements were obtained using a simulated positioner based on a CMOS sensor, while the X-ray energy was modified by changing the voltage, current, and radiation time. A monitoring control unit collected video data of the detected X-rays. The video images were framed and filtered to detect the effective pixel points(radiation spots).The histograms of the images prove there is a linear relationship between the pixel points and X-ray energy. The relationships between the image pixel points, voltage, and current were quantified, and the resultant correlations were observed to obey some physical laws.

关键词： X-ray detection Simulated positioner Complementary metal-oxide-semiconductor sensor Effective pixel points

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Star-shaped conjugated oligoelectrolyte for bioimaging in living cells

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Chinese Science Bulletin 2013年第21期58卷 2570-2575页

作者： SONG WenLi JIANG RongCui yuan yan LU XiaoMei HU WenBo FAN QuLi HUANG Wei Key Laboratory for Organic Electronics & Information Displays (KLOEID) and Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications (NJUPT)

A new star-shaped oligoelectrolyte (TEFCOONa) with triphenylamine as the core, acetylene as linkage and anionic fluorenes as arms was obtained and used for direct imaging in living PANC-1 cells. Because of the hydrophobic conjugated groups of the oli-goelectrolyte, TEFCOONa can form nanospheres with an average diameter of^75 nm in 10 mmol/L PBS. These nanospheres possess a relatively high absolute quantum yield (16.5% in PBS), low cytotoxicity and can penetrate into the nucleus through the cytoplasm, which is essential for living cellular imaging. Collectively, these results validate our rational design of conjugated oligoelectrolyte and even hyper branched polymers-copolyelectrolyte as effective nanovectors for bioimaging and other clinical applications.

关键词：活细胞成像生物成像共轭星形超支化聚合物直接成像平均直径纳米微球

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Can albumin administration relieve lung injury in trauma/hemorrhagic shock?

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World Journal of Gastroenterology 2006年第42期12卷 6884-6888页

作者： Zuo-Bing Chen Zi-Wei Wang Chen-yan Ding Jian-Hua yan yuan Gao Yun Zhang Lin-Mei Ni Yong-Qing Zhou Department of Emergency Surgery First Affiliated Hospital College of Medicine Zhejiang University Hangzhou 310003 Zhejiang Province China

AIM: To study the effect of albumin administration on lung injury in trauma/hemorrhagic shock (T/HS). METHODS: Sixty experimental animals were randomly divided into three groups: rats undergoing laparotomy without shock (T/SS); rats with T/HS and resuscitation with blood plus twice the volume of shed blood as Ringer’s lactate (RL), and rats with T/HS and resuscitation with blood plus additional 3 mL of 50 g/L human albumin. Expression of polymorphonuclear neutrophil (PMN) CD11b/CD18, intercellular adhesion molecule-1 (ICAM-1) of jugular vein blood and the severity of lung injuries [determined mainly by measuring activity of lung tissue myeloperoxidase (MPO) and lung injury score (LIS)] were measured after a 3-h recovery period. RESULTS: All three groups showed a significant difference in the expressions of CD11b/CD18, ICAM-1, and severity of lung injury. The expressions of CD11b/ CD18 in T/SS group, T/HS + RL group, T/HS + albumin group were 17.76% ± 2.11%, 31.25% ± 3.48%, 20.36% ± 3.21%, respectively (F = 6.25, P < 0.05). The expressions of ICAM-1 (U/mL) in T/SS group, T/ HS + RL group, T/HS + albumin group were 258.76 ± 98.23, 356.23 ± 65.6, 301.01 ± 63.21, respectively (F = 5.86, P < 0.05). The expressions of MPO (U/g) in T/SS group, T/HS + RL group, T/HS + albumin group were 2.53 ± 0.11, 4.63 ± 1.31, 4.26 ± 1.12, respectively (F = 6.26, P < 0.05). Moreover, LIS in T/HS + RL group, T/HS + albumin group was 2.62 ± 0.23, 1.25 ± 0.24, respectively. The expressions of CD11b/CD18, ICAM-1 and MPO in T/HS + RL group were significantly increased compared to T/SS group (P = 0.025, P = 0.036, P = 0.028,respectively). However, administration of 3 mL of 50 g/L albumin significantly down-regulated the expressions of CD11b/CD18, ICAM-1 and lung injury index (MPO and LIS) when compared with the T/HS + RL rats (P = 0.035, P = 0.046, P = 0.038, P = 0.012, respectively). CONCLUSION: The infusion of albumin during resuscitation period can protect lung from injury and decrease the e

关键词： Albumin CD11b/CD18 Intercellular adhesion molecule-1 Lung injury Trauma/hemorrhagic shock

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New furo[3,2-h]isochroman from the mangrove endophytic fungus Aspergillus sp. 085242

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Chinese Journal of Natural Medicines 2020年第11期18卷 855-859页

作者： GUO Hui-Xian HUANG Cui-Ying yan Zhang-yuan CHEN Tao HONG Kui LONG Yu-Hua School of Chemistry Guangzhou Key Laboratory of Analytical Chemistry for BiomedicineSouh China Normal UniversiyGuangzhou 510006China Key Laboratory of Combinatorial Biosynthesis and Drug Discovery(Wuhan University) Ministry of Educationand Wuhan University School of Pharmaceutical SciencesWuhan430071China

Four new compounds, asperisocoumarin G(1), asperisocoumarin H(2),(±)-asperisocoumarin I [(±)-3], along with the known pergillin(4) and penicisochroman L(5) were isolated from a mangrove endophytic fungus Aspergillus sp. 085242 by further chemical investigation. The structures of the new compounds, including their absolute configurations, were established by analysis of HR-ESI-MS and NMR spectroscopic data, and ECD calculation. Asperisocoumarins G-I(1-3) were new isocoumarins belonging to the class of furo[3, 2-h]isocoumarins which are rarely found in natural sources. All of the isolated compounds were evaluated for theirα-glucosidase inhibitory effects, and compounds 1 and 4 showed moderate α-glucosidase inhibitory activity, respectively. In an antimicrobial test, the racemate of 3 showed antibacterial activity against Salmonella.

关键词： Aspergillus Furo[3 2-h]isocoumarins α-Glucosidase inhibitory activity Antimicrobial activity

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