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Spatially resolved transcriptomics: a comprehensive review of their technological advances, applications, and challenges

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Journal of Genetics and Genomics 2023年第9期50卷 625-640页

作者： Mengnan Cheng Yujia Jiang Jiangshan Xu Alexios-Fotios AMentis Shuai Wang Huiwen Zheng Sunil kumar sahu Longqi Liu Xun Xu BGI-Hangzhou HangzhouZhejiang 310012China BGI-Shenzhen ShenzhenGuangdong 518103China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Agricultural Genomics BGI-ShenzhenShenzhenGuangdong 518083China Guangdong Provincial Key Laboratory of Genome Read and Write ShenzhenGuangdong 518120China

The ability to explore life kingdoms is largely driven by innovations and breakthroughs in technology,from the invention of the microscope 350 years ago to the recent emergence of single-cell sequencing,by which the scientific community has been able to visualize life at an unprecedented *** recently,the Spatially Resolved Transcriptomics(SRT)technologies have filled the gap in probing the spatial or even three-dimensional organization of the molecular foundation behind the molecular mysteries of life,including the origin of different cellular populations developed from totipotent cells and human *** this review,we introduce recent progresses and challenges on SRT from the perspectives of technologies and bioinformatic tools,as well as the representative SRT *** the currently fast-moving progress of the SRT technologies and promising results from early adopted research projects,we can foresee the bright future of such new tools in understanding life at the most profound analytical level.

关键词： Spatially resolved transcriptomics Bioinformatic tools Spatial single-cell Cell communications Tissue dissection Three-dimensional organ

Platinum Difluoride: A Theoretical and Computational Based Study

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Advances in Biological Chemistry 2023年第6期13卷 236-246页

作者： Anil kumar Soni Vishnu kumar sahu Department of Chemistry Shia P. G. College Lucknow India Department of Chemistry Maharani Lal Kunwari P. G. College Lucknow India

Platinum on reaction with halogen forms three halides viz., platinum (II) chloride, platinum (II) bromide and platinum (II) iodide, except platinum (II) fluoride. In this research work, the not existence of PtF2 has been studied theoretically. For this thermodynamic, valence bond theory and molecular orbital theory based study have also been performed on this molecule. In order to obtain minimum energy structure we optimized the geometry of this halide by opting AM1 for thermodynamic work and EHT for population analysis. All the calculations were performed on CAChe software. The thermodynamic study supported the presumption of disproportion reaction: 2PtF2 →Pt + PtF4. V.B.T showed sd-hybridization rather than sp-hybridization. This was supported by our data as evaluated theoretically by adopting Landis concept, which showed negligible contribution of 5s-orbital of platinum. Mulliken’s population analysis based studies have pointed that the overlap is very poor due to the dis-similarity of energy of combining orbitals of Pt and F atom. The ΣΦ is very small that is 0.2. This also proved that PtF2 failed to match the criteria of overlapping and thus MOT too. Using eigenvalues and population analysis MO diagram has also been drawn, which clearly supported non-existence of PtF2 in nature but its existence in situ and thus also supported the presumption of disproportionation reaction.

关键词： PtF2 Molccecular Mechanics Quantum Mechanism VBT and MOT

Ferroelectric composite-based piezoelectric energy harvester for selfpowered detection of obstructive sleep

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Journal of Materiomics 2023年第4期9卷 609-617页

作者： Swati Panda Hyoju Shin Sugato Hajra Yumi Oh Wonjeong Oh Jeonghyeon Lee P.M.Rajaitha Basanta kumar Panigrahi Jyoti Shukla Alok kumar sahu Perumal Alagarsamy Hoe Joon Kim Department of Robotics and Mechatronics Engineering Daegu Gyeongbuk Institute of Science and Technology(DGIST)Daegu42988South Korea Department of Electrical Engineering Siksha‘O’Anusandhan UniversityBhubaneswar751030India Department of Electrical Engineering Poornima College of EngineeringJaipur303903India Department of Physics Indian Institute of Technology-GuwahatiAssam781039India Robotics and Mechatronics Research Center Daegu Gyeongbuk Institute of Science and Technology(DGIST)Daegu42988South Korea

Lead-free piezoelectric ceramic is a promising material for energy harvesters,as they have superior electromechanical,ferroelectric,and piezoelectric *** addition,piezoelectric ceramics can be blended with polymer to achieve high-flexibility polymer-ceramic composites,providing mechanical robustness and *** this context,a new lead-free ferroelectric material,having the chemical formula SrTi_(2)O_(5)(STO),was synthesized using a high-temperature solid-state *** analyses of the structural,morphological,and electrical properties of the synthesized material were *** crystallizes with orthorhombic symmetry and space group of *** frequency and temperature-dependent dielectric parameters were evaluated,and impedance spectroscopy shed light on the charge *** PDMS-STO composites at different mass fraction of the STO were prepared using a solvent casting route,and a corresponding piezoelectric nanogenerator(PENG)was *** electrical output of the different PENG by varying massfractions of STO in PDMS and varying force were *** 15%(in mass)PENG device delivered the highest peak-to-peak voltage,current,and power density of 25 V,92 nA,and 0.64 mW@500 MU,*** biomechanical energy harvesting using the PENG device by daily human motions,bending of the device,and attaching the device to laboratory equipment was *** the PENG device was attached to the human throat region,and snoring signals were recorded.A classification model was designed employing the convolutional neural network(CNN)*** have been laid to differentiate between normal and abnormal snores,which could help the patient with screening and early disease detection,contributing to self-powered healthcare applications.

关键词： Lead-free Ferroelectric Nanogenerator Sleepdisorder

Adaptive Tracking Control of an Autonomous Underwater Vehicle

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International Journal of Automation and computing 2014年第3期11卷 299-307页

作者： Basant kumar sahu Bidyadhar Subudhi Centre for Industrial Electronics and Robotics Department of Electrical Engineering National Institute of Technology

This paper presents the trajectory tracking control of an autonomous underwater vehicle(AUV). To cope with parametric uncertainties owing to the hydrodynamic effect, an adaptive control law is developed for the AUV to track the desired trajectory. This desired state-dependent regressor matrix-based controller provides consistent results under hydrodynamic parametric *** of the developed controller is verified using the Lyapunov s direct method. Numerical simulations are carried out to study the efficacy of the proposed adaptive controller.

关键词： Autonomous underwater vehicle(AUV) adaptive control law regressor matrix Lyapunovs stability path following

Structural Tuning of Low Band Gap Intermolecular Push/Pull Side-chain Polymers for Organic Photovoltaic Applications

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Chinese Journal of Polymer Science 2017年第9期35卷 1073-1085页

作者： Ansuman Nayak P.S. Rama Sreekanth Santosh kumar sahu Duryodhan sahu Department of Chemistry National Institute of Science and Technology Palur Hills Berhampur Odisha India 761008 Department of Chemistry Gandhi Institute of Engineering and Technology Gunpur Odisha India Department of Mechanical Engineering National Institute of Science and Technology Palur Hills Berhampur Odisha India 761008

A series of novel low band gap donor-acceptor （D-A） type organic co-polymers （BT-F-TPA, BT-CZ-TPA and BT-SI-TPA） consisting of electron-deficient acceptor blocks both in main chains （M1） and at the pendant （M2） were polymerized with different electron rich donor （M3-M5） blocks, i.e., 9,9-dihexyl-9H-fluorene, N-alkyl-2,7-carbazole, and 2,6-dithinosilole, respectively, via Suzuki method. These polymers exhibited relatively low band gaps （1.65-1.88 eV） and broad absorption ranges （680-740 nm）. Bulk heterojunction （BHJ） solar cells incorporating these polymers as electron donors, blended with [6,6]-phenyl-C61-butyric acid methyl ester （PC61BM） or [6,6]-phenyl-Cvl-butyric acid methyl ester （PC71BM） as electron-acceptors in different weight ratios were fabricated and tested under 100 mW/cm2 of AM 1.5 with white-light illumination. The photovoltaic device containing donor BT-SI-TPA and acceptor PC71BM in 1：2 weight ratio showed the best power conversion efficiency （PCE） value of 1.88%, with open circuit voltage （Voc） = 0.75 V, short circuit current density （Jsc） = 7.60 mA/cm2, and fill factor （FF） = 33.0%.

关键词： Polymer solar cells Low band gap Conducting polymers Charge transfer Donor-acceptor Bulk heterojunctionsolar cell

Novel design and composition optimization of self-lubricating functionally graded cemented tungsten carbide cutting tool material for dry machining

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Advances in Manufacturing 2021年第1期9卷 34-46页

作者： Rityuj Singh Parihar Raj kumar sahu Srinivasu Gangi Setti Department of Mechanical Engineering National Institute of Technology RaipurRaipurIndia

The functionally graded cemented tungsten carbide (FGCC) is a suitable material choice for cutting tool applications due to balanced hardness and fracture *** presence of cobalt and CaF2 composition gradient in FGCC may enhance mechanical as well as antifriction ***,structural design of selflubricating FGCC was proposed using Power law composition gradient model and thermal residual stresses (TRSs) as a key ***,*** and *** model was adopted for estimation of TRS,and optimum composition gradient was identified at Power law exponent n =*** designed material displayed compressive and tensile TRS at surface and core respectively;subsequently fabricated by spark plasma sintering and characterized via scanning electron microscope (SEM),indentation *** agreement between experimental and analytical values of TRS demonstrated the effectiveness of intended design model in the composition optimization of self-lubricating *** work will be helpful in implementation of dry machining for clean and green manufacturing.

关键词： Functionally graded cemented tungsten carbide(FGCC) Thermal residual stress(TRS) Solid lubricant Power law

Histological difference in ligament flavum between degenerative lumbar canal stenosis and non-stenotic group:A prospective,comparative study

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World Journal of Orthopedics 2022年第9期13卷 791-801页

作者： Mantu Jain Mukund Sable Amit Purushottam Tirpude Rabi Narayan sahu Sudeep kumar Samanta Gurudip Das Department of Orthopaedics All India Institute of Medical Sciences BhubaneswarBhuabneswar 751019OdishaIndia Department of Pathology All India Institute of Medical Sciences BhubaneswarBhubaneswar 751019OdishaIndia Department of Anatomy All India Institute of Medical Sciences RaipurRaipur 492009ChattisgarhIndia Department of Neurosurgery All India Institute of Medical Sciences BhubaneswarBhubaneswar 751019OdishaIndia

BACKGROUND Ligament flavum(LF)hypertropy is the main etiopathogenesis of lumbar canal stenosis(LCS).The purely elastic LF undergoes a morphological adaptation including a reduction in the elastic fibers and a consequent increase in the collagen content,fibrosis,cicatrization,and ***,the morphometric analysis can delineate the LF in patients with LCS from those without LCS,which would help in better understanding LCS *** To compare the histopathological changes in LF between the degenerative LCS and non-stenotic(non-LCS)*** The present prospective study was conducted in 82 patients who were divided into two groups,namely LCS and *** details of the patients such as duration of symptoms,level of involvement,and number of segments were *** LF obtained from both groups was histopathologically examined for the fibrosis score,elastic fiber degeneration,calcification,and chondroid *** details included a change in elastin and collagen percentages,elastin/collagen ratio,elastic fiber fragmentation,and ligamentocyte *** parameters were compared between the two groups by using the independent t test,Chi-square test,and Pearson’s correlation *** Out of 82 cases,74 were analysed,34 in LCS and 40 in non-LCS *** mean±SD age of presentation in LCS and non-LCS group was 49.2±8.9 and 43.1±14.3 *** LCS group(n=34)exhibited significant differences in fibrosis(P=0.002),elastic fiber degeneration(P=0.01),%elastic fragmentation(66.5±16.3 vs 29.5±16.9),%elastic,content(26.9±6.7 vs 34.7±8.4),%collagen content(63.6±10.4 vs 54.9±6.4),reduction of elastic/collagen(0.4±0.1 vs 0.6±0.1),and ligamentocyte number(39.1±19.1 vs 53.5±26.9)as compared to non-LCS group(n=40).The calcification(P=0.08)and Pearson’s correlation between duration and loss of elastin was not *** difference in LF morphology is consistent in patient’s≥40 years of age among the groups as found in

关键词： Spinal stenosis Lumbar spine Ligamentum flavum histopathology Morphometry

Combustion Characteristics of Sewage Sludge and Algae

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Natural Resources 2016年第4期7卷 205-213页

作者： yaman.kumar sahu Pravin kumar sahu Suryakant Chakradhari Khageshwar Singh Patel School of Studies in Chemistry/Environmental Science Pt. Ravishankar Shukla University Raipur India

Sewage sludge is a good source of energetically desirable compounds and green algae in developing country like India. In this work, the combustion characteristics of sewage sludge and green algae are described. The calorific value (CV) of dried sewage sludge (n = 7) and green algae (n = 11) was ranged from 1800 to 6250 and 3700 to 6280 kcal/kg with mean value of 3999 ± 1347 and 4428 ± 547 kcal/kg, respectively. The effect of proximate variables and water quality parameters in the CV of sludge and algae are discussed.

关键词： Sewage Sludge Algae Water Quality Caloric Value

An Approach to Optimize the Path of Humanoids using Adaptive Ant Colony Optimization

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Journal of Bionic Engineering 2018年第4期15卷 623-635页

作者： Chinmaya sahu Dayal R. Parhi Priyadarshi Biplab kumar Robotics Laboratory Mechanical Engineering Department National Institute of Technology Rourkela-769008 Odisha India

In the emerging area of humanoid robotics, path planning and autonomous navigation have evolved as one of the most promising area of research. This paper deals with the design and development of a novel navigational controller to guide humanoids in cluttered envi- ronments. The basic parameters of the ant colony optimization technique have been modified to have enhanced control as Adaptive Ant Colony Optimization （AACO）. The controller that has been implemented in the humanoids receives sensory information about obstacle distances as inputs and provides required turning angle as output to reach the specified target position. The proposed controller has been tested in both simulated and experimental environments created trader laboratory conditions, and a good agreement has been observed between the simulation and experiment results. Here, both static and dynamic path planning have been attempted. Finally, the proposed controller has also been tested against other existing techniques to validate the efficiency of the AACO in path planning problems.

关键词： navigation path planning humanoid robot AACO Petri-net bionics