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秦川牛宰后成熟过程中线粒体Tu翻译延长因子对牛肉持水性的影响

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食品科学 2025年第1期46卷 1-10页

作者：司健芳张静高爽马佳荣曹松敏李亚蕾罗瑞明宁夏大学食品科学与工程学院宁夏银川750021

探究秦川牛宰后成熟过程中线粒体Tu翻译延长因子(mitochondrial Tu translation elongation factor,TUFM)表达对肉的持水性影响。以秦川牛背最长肌为研究对象,测定4℃不同成熟时间下的pH值、贮藏损失、离心损失、蒸煮损失、水分分布、... 详细信息

探究秦川牛宰后成熟过程中线粒体Tu翻译延长因子(mitochondrial Tu translation elongation factor,TUFM)表达对肉的持水性影响。以秦川牛背最长肌为研究对象,测定4℃不同成熟时间下的pH值、贮藏损失、离心损失、蒸煮损失、水分分布、肌原纤维蛋白等指标变化情况,测定不同成熟时间(0、96、192 h)下TUFM表达量及其含量、Beclin1蛋白表达量。结果显示:在秦川牛宰后成熟期间,肌原纤维蛋白发生降解,TUFM的表达量与Beclin1蛋白表达量和牛肉的持水性存在密切关系,其中蛋白质组学测定的TUFM表达量变化与TUFM含量变化趋势一致,Beclin1蛋白表达量、贮藏损失、离心损失、蒸煮损失整体均呈先上升后下降趋势,pH值呈先下降后上升趋势;Pearson相关性分析表明,牛背最长肌中TUFM表达量与低场核磁共振峰面积比P_(2b)、Beclin1蛋白表达量呈极显著正相关(P0.05)。通过蛋白质组学鉴定出23种与TUFM相关的差异蛋白,通过基因本体论、京都基因与基因组百科全书通路分析发现,差异蛋白可通过多种途径参与能量代谢,进而介导细胞自噬;对差异蛋白和持水性指标进行Pearson相关性分析发现,有5种差异蛋白(ATP5F1D、EEF1A2、GSPT1、NDUFB5、SUCLG1)与持水性指标具有显著相关性(P<0.05、P<0.01)。分析可知,包括TUFM在内,共6种蛋白主要通过能量代谢和氧转运等途径正向或负向影响细胞自噬,从而影响肉的持水性。

关键词：线粒体Tu翻译延长因子持水性蛋白质组学细胞自噬

秦川牛宰后成熟过程中细胞自噬与肉色变化的相关性

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食品科学 2025年第3期46卷 27-35页

作者：梁燕群司健芳马萌高爽李亚蕾罗瑞明宁夏大学食品科学与工程学院宁夏大学动物科技学院

为探究秦川牛宰后成熟过程中细胞自噬的发生情况及其对肉色的影响，以秦川牛背最长肌为研究对象，分析4℃环境下不同成熟时间段样品的肉色、肌红蛋白相对含量、高铁肌红蛋白还原酶活性（metmyoglobin reductase activity,MRA）、苄氯素1... 详细信息

为探究秦川牛宰后成熟过程中细胞自噬的发生情况及其对肉色的影响，以秦川牛背最长肌为研究对象，分析4℃环境下不同成熟时间段样品的肉色、肌红蛋白相对含量、高铁肌红蛋白还原酶活性（metmyoglobin reductase activity,MRA）、苄氯素1(Beclin1)、微管相关蛋白1轻链3(microtubule-associated protein light chain 3-Ⅱ,LC3-Ⅱ)、自噬相关蛋白7(autophagy-related protein 7,ATG7)、螯合体1(sequestosome 1,p62)、半胱天冬酶8(caspase 8)、线粒体膜电位（mitochondrial membrane potential,MMP）、活性氧（reactive oxygen species,ROS）等指标变化情况，结果发现随着成熟时间的延长，L*值、a*值和Beclin1、LC3-Ⅱ、ATG7蛋白表达量呈先上升后下降趋势，caspase 8、p62、ROS呈先下降后上升趋势，脱氧肌红蛋白、氧和肌红蛋白相对含量、MMP、MRA呈下降趋势，b*值、高铁肌红蛋白相对含量呈上升趋势。在宰后成熟过程中细胞自噬关键蛋白均不同程度地表达，肌肉细胞产生了自噬现象，其中96 h牛肉细胞自噬活动最强；对细胞自噬关键蛋白进行的相关性分析显示，各蛋白之间均呈极显著相关（Plin1、LC3-Ⅱ、ATG7、p62、caspase 8可作为检测宰后成熟过程中肉色变化的生物标记物。

关键词：细胞自噬线粒体功能苄氯素1 微管相关蛋白1轻链3 肉色

Development of hedgehog pathway inhibitors by epigenetically targeting Gli through BET bromodomain for the treatment of medulloblastoma

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Acta Pharmaceutica Sinica B 2021年第2期11卷 488-504页

作者： Xiaohua liu Yu Zhang yalei li Juan Wang Huaqian Ding Wenjing Huang Chunyong Ding Hongchun liu Wenfu Tan Ao Zhang CAS Key Laboratory of Receptor Research Shanghai Institute of Materia Medica(SIMM)Chinese Academy of SciencesShanghai 201203China Department of Pharmacology School of PharmacyFudan UniversityShanghai 201203China State Key Laboratory of Drug Research Shanghai Institute of Materia Medica(SIMM)Chinese Academy of SciencesShanghai 201203China School of Pharmacy Shanghai Jiao Tong UniversityShanghai 200240China School of life Science and Technology ShanghaiTech UniversityShanghai 201210China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Esophageal Cancer Prevention and Treatment Ministry of Education of ChinaZhengzhou UniversityZhengzhou 450001China

Medulloblastoma(MB)is a common yet highly heterogeneous childhood malignant brain tumor,however,clinically effective molecular targeted therapy is *** of hedgehog(HH)signaling by epigenetically targeting the transcriptional factors Gli through bromodomain-containing protein 4(BRD4)has recently spurred new interest as potential treatment of HH-driven *** screening of current clinical BRD4 inhibitors for their inhibitory potency against glioma-associated oncogene homolog(Gli)protein,the BRD4 inhibitor 2 was selected as the lead for further structural optimization,which led to the identification of compounds 25 and 35 as the high potency HH *** profiling showed that both compounds suppressed HH signaling by interacting with the transcriptional factor Gli,and were equally potent against the clinical resistant mutants and the wild type of smoothened(SMO)receptor with IC50 values around 1 nmol/*** the resistant MB allograft mice,compound 25 was well tolerated and markedly suppressed tumor growth at both 5 mg/kg(TGI=83.3%)and 10 mg/kg(TGI=87.6%)*** further modification is needed to improve the pharmacokinetic(PK)parameters,compound 25 represents an efficacious lead compound of Gli inhibitors,possessing optimal safety and tolerance to fight against HH-driven MB.

关键词： Medulloblastoma Hedgehog signaling pathway Drug resistance Gli BRD4

A comparison between benthic gillnet and bottom trawl for assessing fish assemblages in a shallow eutrophic lake near the Changjiang River estuary

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Journal of Oceanology and limnology 2018年第2期36卷 572-586页

作者： li yalei liU Qigen CHEN liping ZHAO lianjie WU Hao CHEN liqiao HUT Zhongjun Key laboratory of Freshwater Fishery Germplasm Resources Ministry of Agriculture Shanghai Ocean University Shanghai 201306 China Xinyang College of Agriculture and Forestry Xinyang 464000 China Nanjing Institute of Environmental Science Ministry of Environmental Protection Nanjing 210042 China School of life Science East China Normal University Shanghai 200062 China

Two fishing methods including gillnetting and trawling to estimate attributes of fish assemblage were compared in Dianshan Lake from August 2009 to July 2010. Species composition dif fered significantly between the gears, with four significant contributors in gillnet catches and one in trawl catches. Trawling collected more proportions of benthic species by number and biomass than gillnetting. Size distribution was significantly influenced by fishing technique; gillnetting captured relatively less small-sized fishes and trawling captured less large-sized individuals. Trawling produced species richness closer to the one expected than gillnetting. On the whole, trawl catch was a quadratic polynomial function of gillnet catch and a significantly negative correlation was found between them, both of which varied as dif ferent polynomial functions of temperature. However, trawl and gillnet catches were significantly correlated only in one of five month groups. It is concluded that single-gear-based surveys can be misleading in assessments of attributes of fish assemblages, bottom trawling is a more ef fective gear for assessing fish diversity than benthic gillnetting, and using gillnet catches as an indicator of fish density depends on fishing season in the lake.

关键词： fishing gear expected species richness size structure catch per unit effort temperature

Application of Multicast-based Video Conference on CERNET Backbone

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计算机工程 2002年第S1期28卷 133-136页

作者： ZHANG Xuan, WANG yalei, li Chongrong(Network Research Center of Tsinghua University, Beijing 100084,China) Network Research Center of Tsinghua University Beijing 100084China

Multicast-based video conference is a representative application in advanced network. In multi-point video conference using multicast can get better efficiency facilitated by inner-group broadcast mechanism. In the application, the multicast-based network resources assignment, management and security should be considered together. This paper presents a framework model of multicast-based video conferencing application with three layers. And a practical multicast-based video conferencing is implemented in CERNET(China Education and Research Network) backbone. The practice is valuable for the development of multicast-based video conferencing application in China.

关键词： Multicast Video conference Network application

宰后贮藏期间氧化磷酸化对秦川牛肉色泽的影响

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中国食品学报 2023年第10期23卷 249-260页

作者：张萌李亚蕾宁夏大学食品与葡萄酒学院银川750021

为揭示宰后贮藏过程中牛肉肉色变化机制,以25月龄秦川牛背最长肌为研究对象,测定其贮藏0,4,8 d时pH值、肉色和肌红蛋白相对含量的变化情况。基于4D-非标记蛋白质组学技术研究贮藏期间氧化磷酸化代谢通路蛋白质表达量与肌红蛋白表达量之... 详细信息

为揭示宰后贮藏过程中牛肉肉色变化机制,以25月龄秦川牛背最长肌为研究对象,测定其贮藏0,4,8 d时pH值、肉色和肌红蛋白相对含量的变化情况。基于4D-非标记蛋白质组学技术研究贮藏期间氧化磷酸化代谢通路蛋白质表达量与肌红蛋白表达量之间的关系。结果表明:随着贮藏时间的延长,pH值呈先降低后升高的趋势。a^(*)值逐渐降低,b^(*)值逐渐升高,L^(*)值呈先上升后下降的趋势。氧合肌红蛋白相对含量显著下降(P<0.05),高铁肌红蛋白相对含量显著上升(P<0.05),脱氧肌红蛋白相对含量变化不显著。肌红蛋白表达量在0~4 d上调,4~8 d下调。氧化磷酸化代谢通路上的关键蛋白质ATP5F1D贮藏初期上调,NDUFA4、NDUFB5贮藏后期下调,且氧化磷酸化代谢通路上的关键蛋白对牛背最长肌pH值、肉色均有显著影响。L*值与DeoMb相对含量呈极显著正相关(P<0.01),与OxyMb相对含量呈极显著负相关(P<0.01),与ATP5F1D呈极显著正相关(P<0.01)。NDUFA5与高铁肌红蛋白相对含量呈显著负相关(P<0.05)。因此,探究氧化磷酸化代谢通路关键蛋白质对肉色的影响,对于宰后肉品品质形成具有重要调控作用。

关键词：秦川牛背最长肌品质氧化磷酸化 4D-非标记蛋白质组学冷藏

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单细胞转录组测序及其在神经系统研究中的应用

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基因组学与应用生物学 2019年第10期38卷 4490-4496页

作者：李亚雷南昌大学生命科学学院

哺乳动物神经系统的转录组学研究,可以从分子水平分析基因表达模式,并作为细胞形态学和电生理学等传统研究方法的补充。传统的多细胞转录组测序只能获取细胞群体的平均特征,掩盖了单个细胞之间的异质性。单细胞转录组测序可以分析神经... 详细信息

哺乳动物神经系统的转录组学研究,可以从分子水平分析基因表达模式,并作为细胞形态学和电生理学等传统研究方法的补充。传统的多细胞转录组测序只能获取细胞群体的平均特征,掩盖了单个细胞之间的异质性。单细胞转录组测序可以分析神经系统中的基因表达,发现其中的可变剪切和新转录本,为神经系统的分子分类以及探究神经退化机制等方面带来更深层次的见解。本综述从不同的角度综述了最新的单细胞转录组测序方法,讨论了各个方法的适用性,着重对单细胞转录组测序在神经系统中的应用现状进行了详细阐述。

关键词：单细胞转录组测序神经细胞异质性分子分类神经性疾病

不同激光脉冲作用下ZnS晶体非线性光学特性

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红外与激光工程 2015年第5期44卷 1534-1538页

作者：常青李亚蕾黑龙江大学电子工程学院黑龙江哈尔滨150080

用Z-scan技术在532nm的皮秒激光脉冲和800nm飞秒激光脉冲作用下分别研究了ZnS晶体的非线性吸收及非线性折射特性。实验结果表明,ZnS晶体在532nm的皮秒激光脉冲作用下非线性吸收为双光子吸收,其非线性吸收系数为5.3×10-11m/W,在800nm的... 详细信息

用Z-scan技术在532nm的皮秒激光脉冲和800nm飞秒激光脉冲作用下分别研究了ZnS晶体的非线性吸收及非线性折射特性。实验结果表明,ZnS晶体在532nm的皮秒激光脉冲作用下非线性吸收为双光子吸收,其非线性吸收系数为5.3×10-11m/W,在800nm的飞秒激光脉冲作用下非线性吸收为三光子吸收,非线性吸收系数为0.59×10-21m2/W2;在532nm皮秒激光脉冲作用下,ZnS晶体的非线性折射率符号为负,自由载流子产生的非线性折射率的改变占主导,而800nm飞秒激光脉冲作用下,ZnS晶体的非线性折射率符号为正,束缚电子产生的非线性折射率的改变为主要因素。

关键词： ZnS晶体非线性吸收非线性折射

Effect of rare earth element cerium on preparation of tungsten powders

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Journal of Rare Earths 2015年第5期33卷 561-566页

作者：何文谭敦强李亚蕾杨欣陆磊陆德平 School of Materials Science and Engineering Nanchang University Institute of Applied Physics Jiangxi Academy of Sciences

Tungsten powders and Ce doped powders were prepared by hydrogen reduction combined with the liquid-solid doping method. The phase composition, particle size and powder morphology of Ce doped tungsten powders were analyzed by X-ray diffrac-tion, scanning electron microscopy and transmission electron microscopy, respectively. The results indicated that 10000 ppm Ce doped tungsten oxide powders were consisted of WO3 phase and Ce4W9O33 phase. The hydrogen reduction of Ce doped tungsten powders was basically accomplished at 800 oC for 3 h. The size of Ce doped W powders was remarkably decreased compared to the undoped W powders. The phase of Ce4W9O33 was reduced to Ce2 （WO4）3 phase and Ce2W2O9 phase during the process of hydrogen reduction. Moreover, Ce2 （WO4）3 phase and Ce2W2O9 phase were observed form their morphologies, where the doping content of Ce was more than 100 ppm. The ternary phase embedding into W particles was assigned to Ce2 （WO4）3, while the ternary phase distrib-uting among W particles corresponded to Ce2W2O9. The phase of Ce2 （WO4）3 might be the nucleus of W particles and increase the number of the nucleus. And the particles of Ce2W2O9 covered WO2 particles and might inhibit the growth of W particles. These two reasons resulted in the decrease of the size of Ce doped W particles. Uniform fine W powders were fabricated with the doping content of Ce more than 100 ppm.

关键词： cerium tungsten oxide hydrogen reduction tungsten powders rare earths