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水流对斑马鱼体色和肥满度的影响

上海海洋大学学报 2024年第5期33卷 1064-1073页

作者：丁长晨黄亚娟司玉凤鲍宝龙上海海洋大学水产种质资源发掘与利用教育部重点实验室上海201306 上海海洋大学水产科学国家级实验教学示范中心上海201306

为了探究水流对鱼类体色的影响,比较了不同流速水流养殖下斑马鱼皮肤黑色素细胞数量和基因的表达,结果表明:相对于低流速(0.010 m/s),在高流速(0.022 m/s)下养殖斑马鱼3个月后,皮肤黑色素细胞数量显著增加,尤其是鱼体尾部区域;实时荧光... 详细信息

为了探究水流对鱼类体色的影响,比较了不同流速水流养殖下斑马鱼皮肤黑色素细胞数量和基因的表达,结果表明:相对于低流速(0.010 m/s),在高流速(0.022 m/s)下养殖斑马鱼3个月后,皮肤黑色素细胞数量显著增加,尤其是鱼体尾部区域;实时荧光定量PCR(RT-qPCR)结果显示高流速组斑马鱼皮肤黑色素细胞标记基因kita、mitfa和tyrp1a相对表达量显著升高,黄色素细胞标记基因csf1ra显著降低,虹彩细胞标记基因pnp4a无显著变化,表明高流速水流诱导黑色素细胞而抑制黄色素细胞的形成。RT-qPCR结果显示,相比于低流速组,高流速组斑马鱼皮肤中黑色素细胞形成抑制因子基因asip1的相对表达量显著减少,与黑色素细胞形成相关的黑皮质素受体基因mc1r、阿黑皮素原基因pomca无显著性变化,视黄酸合成相关的视黄醛脱氢酶基因raldh2和raldh3显著下调,提示水流刺激可通过降低asip1的表达来诱导黑色素细胞形成;水流刺激瞬时受体通道蛋白基因trpv4和压电式机械敏感通道蛋白基因piezo2的表达上调,而piezo1无显著性变化。以上结果表明,水流能诱导斑马鱼黑色素细胞的形成,可能是通过皮肤机械力感受蛋白Trpv4或Piezo2介导水流刺激减少asip1表达的结果。Asip1除了调控黑色素细胞形成以外还调控脂肪的积累,相比于低流速组,高流速组斑马鱼肥满度也显著下降,GO富集分析显示差异表达基因主要富集到甘油三酯分解代谢、高密度脂蛋白颗粒、脂质结合等条目,差异表达基因显著富集的KEGG通路有:氨基糖和核苷酸糖的代谢、PPAR信号通路、亚油酸代谢、花生四烯酸代谢等。RT-qPCR检测与脂肪代谢相关的脂肪酸过饱和酶2基因fads2显著上调,瘦素基因lep无显著性差异,提示水流可能通过Asip1影响Fads2进而影响脂肪降解。本研究首次报道了水流对鱼类体色的影响,为理解目前越来越普遍的流水养殖对鱼类体色和肥满度的影响提供了初步的理论基础。

关键词：水流斑马鱼体色肥满度机械力感受蛋白

镍氢、锂离子电池回收利用项目环境影响评价关键问题探讨

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当代化工研究 2024年第1期 74-76页

作者：黄雅娟刘光生蓝伟锋厦门市环境监测站福建361000 厦门理工学院环境科学与工程学院福建361024 福建省环安检测评价有限公司福建361000

随着新能源汽车产业的高速发展,作为绿色电池时代典型代表的镍氢和锂离子电池受到广泛的运用,也带来大量的报废动力电池。这些废旧电池若无法安全处理,将对社会环境安全造成重大影响。本文在实际开展镍氢和锂离子电池回收项目环境影响... 详细信息

随着新能源汽车产业的高速发展,作为绿色电池时代典型代表的镍氢和锂离子电池受到广泛的运用,也带来大量的报废动力电池。这些废旧电池若无法安全处理,将对社会环境安全造成重大影响。本文在实际开展镍氢和锂离子电池回收项目环境影响评价的基础上,分析项目污染物的产生情况,并据此探讨了环评中的几个关键问题--工程分析、清洁生产、环境经济效益,并提出此类项目关注的要点及可行的污染治理措施,以期为电池回收项目建设和环境评价管理提供依据。

关键词：电池回收利用环境影响评价污染防治措施经济环境效益

Half Smooth Tongue Sole(Cynoglossus semilaevis) Under Low Salinity Stress Can Change Hepatic igf2 Expression Through DNA Methylation

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Journal of Ocean University of China 2020年第1期19卷 171-182页

作者： LI Siping HE Feng WEN Haishen SI Yufeng LIU Mingyuan huang yajuan WU Shuxian Fishery College the Key Laboratory of Mariculture of Ministry of EducationOcean University of ChinaQingdao 266003China

Salinity is a crucial environmental stress that severely affects fish growth and *** environmental stress,DNA methylation plays an important role in gene expression and genome *** better understand the epigenetic regulation mechanism of igf2 under low salinity stress,we analyzed the DNA methylation at 5’UTR,exon1,intron1,and exon2,and analyzed the relationship of DNA methylation with mRNA abundance as well as the special single CpG sites methylation patterns of igf2 in the liver of half smooth tongue sole under low salinity(15)for 0,7,and 60 *** exposed to low salinity,DNA methylation at 5’UTR and exon2 remained stable,while it was up-regulated firstly and then down-regulated at exon1 and *** single CpG sites of igf2 associated with low salinity,and most of these sites with significantly changed methylation levels(P<0.05)are located in intron1 *** discrepant variation of single CpG sites methylation levels and igf2 expression further revealed that females and males showed different response to low ***,the 38-CpG site of intron1 servers as a sexual ***,our integrative analysis demonstrated that regional DNA of igf2 methylation had highly complex interplay on gene *** single CpG sites in intron1 were indispensable epigenetic markers under external environmental *** all,to resist the low salinity stress,half smooth tongue sole liver can regulate the expression of igf2 through methylation of CpG sites in intron1.

关键词： salinity hepatic igf2 DNA methylation gene expression half smooth tongue sole

The role of nitric oxide and neuronal nitric oxide synthase in zebrafish(Danio rerio)shoaling

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Aquaculture and Fisheries 2021年第6期6卷 565-573页

作者： Rachit Penglee Lei Gao yajuan huang Liping Liu Sukkrit Nimitkul Baolong Bao Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University) Ministry of EducationInternational Research Center for Marine Biosciences at Shanghai Ocean UniversityMinistry of Science and TechnologyNational Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghai201306China Department of Aquaculture Faculty of FisheriesKasetsart UniversityChatuchakBangkok10900Thailand

Nitric oxide(NO)-the product of arginine metabolism catalyzed by nitric oxide synthases(NOS)-is a well-known neurotransmitter which plays an important role in metabolism and amino acid transportation in the nervous *** particular,it can inhibit monoamine neurotransmitter transportation which affects animal behavior,especially social ***-is a one kind of social *** is a behavior that individual fish choose to join with their group within two factors;food and predation *** fish has quickly responded to predator and increased the change in feeding *** addition,shoaling also effect to stress response on stock density of aquaculture *** effect of NO molecular signaling on the dopamine pathway was investigated using zebrafish(Danio rerio)as a model *** aim was to understand the role of NOS and NO in shoaling behavior,which is typical of *** concentration of NO in the zebrafish brain was modulated using a knockout for the neuronal NOS gene,and NO production was induced through treatment with *** existence of NO in the zebrafish brain was confirmed by using a fluorescent *** concentration in the brain was measured by UPLC tandem mass *** measured shoaling cohesion of all individual fish of ***,using average distance between all pairs of fish(nearest neighbor distance)and analyzed tracking by Zebralab ViewPoint ***,our results suggest that a lower level of NO was associated with a higher level of dopamine,which in turn leads to the shoaling behavior.

关键词： Nitric oxide Neuronal nitric oxide synthase Shoaling Dopamine transporter Neurotransmitter

Methylation Status of the Follistatin Gene at Different Development Stages of Japanese Flounder(Paralichthys olivaceus)

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Journal of Ocean University of China 2018年第5期17卷 1243-1252页

作者： huang yajuan HU Nan SI Yufeng LI Siping WU Shuxian ZHANG Meizhao WEN Haishen LI Jifang LI Yun HE Feng The Key Laboratory of Mariculture of Ministry of Education Fishery CollegeOcean University of China

Follistatin （Fst） is a hyperplasia factor that plays a crucial role in muscle development. DNA methylation, a significant process, regulates gene expression. The aim of our study is to examine the DNA methylation and expression patterns ofFst gene at five different development stages of Japanese flounder （stage A, 7 dph; stage B, 90 dph; stage C, about 180 dph; stage D, about 24 months; stage E, about 36 months）. The muscle tissue of Japanese flounder was obtained at different development stages in dais experiment. DNA methylation levels in the promoter and exon 2 of Fst were determined by bisulfite sequencing, and the relative expression of the Fst gene at the five stages was measured by quantitative PCR. The results showed that the lowest methylation level was at stage A and the highest methylation level was at stage B. Moreover, the highest expression level of the Fst gene was observed at stage A. The mRNA abundance was negatively correlated with DNA methylation level. Three CpG islands in the promoter region and three CpG islands in exon 2 of Fst were found in the binding sequence of the putative transcription factor. These results offered a theoretical basis for the mechanism of Fst gene regulation to muscle development at different development stages.

关键词： Japanese flounder Follistatin DNA methylation gene expression muscle growth

Machine learning and neural network supported state of health simulation and forecasting model for lithium-ion battery

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Frontiers in Energy 2024年第2期18卷 223-240页

作者： Nan QI Kang YAN yajuan YU Rui LI Rong huang Lai CHEN Yuefeng SU Beijing Institute of Technology Chongqing Innovation Center Chongqing 401120China Department of Energy and Environmental Materials School of Materials Science and EngineeringBeijing Institute of TechnologyBeijing 100081China Beijing Electric Vehicle Co. Ltd.Beijing 100176China

As the intersection of disciplines deepens,the field of battery modeling is increasingly employing various artificial intelligence(AI)approaches to improve the efficiency of battery management and enhance the stability and reliability of battery *** paper reviews the value of AI methods in lithium-ion battery health management and in particular analyses the application of machine learning(ML),one of the many branches of AI,to lithium-ion battery state of health(SOH),focusing on the advantages and strengths of neural network(NN)methods in ML for lithium-ion battery SOH simulation and *** is one of the important branches of ML,in which the application of NNs such as backpropagation NN,convolutional NN,and long short-term memory NN in SOH estimation of lithium-ion batteries has received wide *** so far have shown that the utilization of NN to model the SOH of lithium-ion batteries has the advantages of high efficiency,low energy consumption,high robustness,and scalable *** the future,NN can make a greater contribution to lithium-ion battery management by,first,utilizing more field data to play a more practical role in health feature screening and model building,and second,by enhancing the intelligent screening and combination of battery parameters to characterize the actual lithium-ion battery SOH to a greater *** in-depth application of NN in lithium-ion battery SOH will certainly further enhance the science,reliability,stability,and robustness of lithium-ion battery management.

关键词： machine learning lithium-ion battery state of health neural network artificial intelligence

氧化焙烧—铅试金—电感耦合等离子体原子发射光谱法测定粗硒中金银铂钯

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黄金 2024年第3期45卷 94-97页

作者：黄雅娟刘芳美陈兴发甘聪钟坚海紫金铜业有限公司福建省铜绿色生产及伴生资源综合利用重点实验室厦门海关技术中心

为准确、快速测定粗硒中金、银、铂、钯的含量,建立了氧化焙烧除硒、铅试金法结合电感耦合等离子体原子发射光谱法测定粗硒中金、银、铂、钯的方法。实验采用二氧化硅铺底,阶段升温至600℃,焙烧时间2 h,除硒效果较好,且不会造成金、银... 详细信息

为准确、快速测定粗硒中金、银、铂、钯的含量,建立了氧化焙烧除硒、铅试金法结合电感耦合等离子体原子发射光谱法测定粗硒中金、银、铂、钯的方法。实验采用二氧化硅铺底,阶段升温至600℃,焙烧时间2 h,除硒效果较好,且不会造成金、银、铂、钯的损失;当试样量为10.0 g,试金硅酸度为1.0时,熔渣流动性良好,能够得到光滑圆润的贵金属合粒,经硝酸溶解后以盐酸沉淀分离银;选择Pt 265.945 nm、Pd 340.458 nm作为测定谱线进行电感耦合等离子体原子发射光谱法测定,铂和钯校准曲线的线性相关系数均大于0.999,检出限分别为0.0020 mg/L和0.0018 mg/L,测定下限分别为0.0067 mg/L和0.0061 mg/L。本方法测定结果的相对标准偏差(RSD,n=7)为0.47%~5.9%,加标回收率为97.3%~103.0%,准确度和精密度满足生产控制要求。

关键词：氧化焙烧粗硒铅试金电感耦合等离子体原子发射光谱法金银铂钯

Wearable pressure sensors based on antibacterial and porous chitosan hydrogels for full-range human motion detection

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Science China(Technological Sciences) 2024年第8期67卷 2475-2484页

作者： ZHANG YingXin LIU HanGuang JIN ShunYu LI yajuan ZHANG Wan ZHOU Hang NIE ZanXiang huang Yuan School of Microelectronics Science and Technology Sun Yat-sen UniversityGuangzhou 510275China Hefei National Research Center for Physical Sciences at the Microscale University of Science and Technology of ChinaHefei 230022China Department of Clinical Laboratory the First Affiliated Hospital of Anhui Medical UniversityHefei 230022China School of Electronic and Computer Engineering Peking University Shenzhen Graduate SchoolShenzhen 518055China Zinergy Shenzhen Ltd. Shenzhen 518109China

Wearable pressure sensors made from conductive hydrogels hold significant potential in health ***,limited pressure range(Pa to hundreds of kPa)and inadequate antibacterial properties restrict their practical applications in diagnostic and health ***,a wearable high-performance pressure sensor was assembled using a facilely prepared porous chitosan-based hydrogel,which was constructed from commercial phenolphthalein particles as a sacrificial *** relationship between the porosity of hydrogels and sensing performance of sensors was systematically ***,the wearable pressure sensor,featuring an optimized porosity of hydrogels,exhibits an ultrawide sensing capacity from 4.83 Pa to 250 k Pa(range-to-limit ratio of 51,760)and high sensitivity throughout high pressure ranges(0.7 kPa~(-1),120–250 kPa).The presence of chitosan endows these hydrogels with outstanding antibacterial performance against *** and ***,making them ideal candidates for use in wearable *** features allow for a practical approach to monitor full-range human motion using a single device with a simple structure.

关键词： porous hydrogels antibacterial performances wearable pressure sensors full-range human motion chitosan

Dynamic modulation of nodulation factor receptor levels by phosphorylation-mediated functional switch of a RING-type E3 ligase during legume nodulation

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Molecular Plant 2024年第7期17卷 1090-1109页

作者： Hao Li yajuan Ou Jidan Zhang Kui huang Ping Wu Xiaoli Guo Hui Zhu Yangrong Cao National Key Lab of Agricultural Microbiology Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China National Key Lab of Agricultural Microbiology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China

The precise control of receptor levels is crucial for initiating cellular signaling transduction in response to specific ligands;however,such mechanisms regulating nodulation factor(NF)receptor(NFR)-mediated perception of NFs to establish symbiosis remain *** this study,we unveil the pivotal role of the NFR-interacting RING-type E3 ligase 1(NIRE1)in regulating NFR1/NFR5 homeostasis to optimize rhizobial infection and nodule development in Lotus *** demonstrated that NiRE1 has a dual function in this regulatory *** associates with both NFR1 and NFR5,facilitating their degradation through K48-linked polyubiquitination before rhizobial ***,following rhizobial inoculation,NFR1 phosphorylates NIRE1ata conserved residue,Tyr-109,inducing a functional switch in NIRE1,which enables NIRE1tomediateK63-linkedpolyubiquitination,thereby stabilizing NFR1/NFR5 in infected root *** introduction of phospho-dead NIRE1Y1osF leads to delayed nodule development,underscoring the significance of phosphorylation at Tyr-1o9 in orchestrating symbiotic ***,expression of the phospho-mimic NIRE1Y0E results in the formation of spontaneous nodules in ***,further emphasizing the critical role of the phosphorylation-dependent functional switch in *** summary,these findings uncover a fine-tuned symbiotic mechanism that a single E3 ligase could undergo a phosphorylationdependent functional switch to dynamically and precisely regulate NF receptor protein levels.

关键词： nodule symbiosis nodulation factor receptors E3 ligase ubiquitination phosphorylation protein degradation protein stabilization Lotus japonicus