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Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

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Science China Chemistry 2025年第1期 308-316页

作者： Sili wang Jiancheng Huang yanan Du Wei Huang Yufeng Xue Xiaokun Xu Guannan Zhong Yuanyuan Shi Bin Hong Xiaoying Bian wen Liu State Key Laboratory of Chemical Biology Shanghai Institute of Organic Chemistry University of Chinese Academy of SciencesChinese Academy of Sciences State Key Laboratory of Microbial Technology Shandong University National Health Commission (NHC) Key Laboratory of Biotechnology for Microbial Drugs Institute of Medicinal BiotechnologyChinese Academy of Medical Science & Peking Union Medical College

Cobalamin(Cbl)-dependent radical S-adenosyl-L-methionine(SAM) proteins constitute the largest collection of the radical SAM superfamily that has hundreds of thousands of individual members. Many of these proteins are involved in the biosynthesis of pharmaceutically important natural products to catalyze chemically demanding reactions. In the biosynthetic pathway of chuangxinmycin(CXM), a unique indole alkaloid antibiotic with potent anti-infective activity, functionalization of the characteristic thiopyrano[4,3,2-cd]indole scaffold by regio-and stereoselective C3-methylation is believed to rely on a Cbl-dependent radical process, which, however, remained to be reconstituted biochemically. We here report the dissection of this enzymatic process, which requires the incorporation of Cxm8, a Cbl-dependent radical SAM protein, with Cxm9, a DUF5825family protein that shares no homology to any proteins of known functions. Cxm8 and Cxm9 function together by forming an unexpected heterodimeric complex that selectively catalyzes C3-methylation of the tricyclic indole-S-hetero ring system in a successive manner, achieving CXM and a recently identified, C3-dimethylated congener. Detailed biochemical characterization,isotope labeling, structural simulation and bioinformatics analysis rationalized the catalysis of the Cxm8/Cxm9 complex and particularly the necessity of the DUF5825 protein for C3-methylase activity. This is the first example that a Cbl-dependent protein acts with a partner to exhibit radical SAM activity.

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A Study on the Effect Factors of Community Participation in Tourism:Based on the Logistic Regression Analysis of Xingwen World Geopark

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四川地质学报 2009年第S1期29卷 120-123,132页

作者： SHUI Wei 1,XU Xue-yi 1,WEI ya-li 2,CHEN Yin 3,wang Xing-gui 4,ZHOU Hu 1,wang ya-wen 2,wang Li 5,OU yaNG Shu-jian 2(1.Department of Eco-agriculture and Rural Development,Sichuan Agricultural University,ya’an 625014,Sichuan 2.College of Resources and Environment,Sichuan Agricultural University,ya’an 625014,Sichuan 3.Xingwen Science and Technology Bureau,YIBin 644400,Sichuan 4.Environment and Life Science of Kangding Nationality Teachers College,Kingding 626001,Sichuan 5.School of Land and Resources,China West Normal University,Nanchong 637002,Sichuan.)

In order to find out the factors which influence community participation in tourism,several effect factors are summed up through the investigation of the local residents and the analysis of the logistic regression of the data gained from Xingwen world *** indicated there are several main influencing factors of community participation such as the comparative benefit of tourist industry,participant residents' subjective wishes,preference consciousness of working opportunity obtainment in the tourist enterprise and participant *** on above investigation data and field travel,the paper put forward several methods about how to enhance the residents' participant degree:increasing the comparative benefit of tourist industry,enhancing the attraction of tourism for boosting participant wishes,improving the preference consciousness on obtaining working opportunity in tourism industry and cultivating residents' participant skills through training.

关键词： Community participation Effect factors Logistic regression Xngwen World Geopark

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后交叉韧带胫骨附着点撕脱骨折:关节镜治疗中的材料、植入物及内固定技术

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中国组织工程研究 2025年第4期29卷 872-880页

作者：余铭王文贵州医科大学贵州省贵阳市550004 暨南大学附属广州市红十字会医院广东省广州市510220

背景:治疗后交叉韧带胫骨附着点撕脱骨折的最佳手术技术仍值得商榷。随着关节镜手术的应用与成熟,它在后交叉韧带胫骨附着点撕脱骨折的诊疗中有很大前景。目的:综述关节镜技术在后交叉韧带胫骨附着点撕脱骨折治疗中的应用与进展,包括不... 详细信息

背景:治疗后交叉韧带胫骨附着点撕脱骨折的最佳手术技术仍值得商榷。随着关节镜手术的应用与成熟,它在后交叉韧带胫骨附着点撕脱骨折的诊疗中有很大前景。目的:综述关节镜技术在后交叉韧带胫骨附着点撕脱骨折治疗中的应用与进展,包括不同关节镜治疗方法、手术入路、胫骨隧道设计、缝合材料选择以及内固定植入物选择等。方法:通过计算机对中国知网、PubMed、Web of Science及ScienceDirect等数据库中的相关文献进行检索,检索时间为2003年1月至2023年11月,中文检索词为“后交叉韧带,后十字韧带,撕脱骨折,关节镜”;英文检索词为“posterior cruciate ligament,avulsion,fracture,tibia,arthroscopic,operation,fixation,treatment”。共纳入97篇文献进行综述。结果与结论:关节镜技术提供了一种可靠的治疗方式来治疗后交叉韧带胫骨附着点撕脱骨折。根据入路、缝合材料类型以及用于缝合的入路和胫骨隧道数量等不同,关节镜技术可以分为关节镜下缝线固定结合自体移植物增强重建、关节镜下多交叉带缝合桥固定、关节镜下高强度缝线固定以及关节镜下直接前后缝合悬吊固定等几类。在各种研究中,常用的临床结果评估指标包括关节活动度、Lysholm评分、国际膝关节文献委员会评分及KT-2000关节测量仪差等,研究显示关节镜手术后末次随访时上述指标检测结果较术前显著改善,影像学随访结果显示关节镜手术都取得了令人满意的结果。在随访过程中,接受关节镜技术治疗后的各类交叉韧带胫骨附着点撕脱骨折患者都未出现严重并发症,例如创伤性关节炎、神经血管损伤、围手术期伤口感染、血栓形成以及骨折不愈合等。

关键词：后交叉韧带胫骨撕脱骨折关节镜手术缝合材料内固定治疗

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Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： Song He Li Li Sai wang Shao-Jiang wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

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Local Well-posedness for Linearized Degenerate MHD Boundary Layer Equations in Analytic Setting

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Acta Mathematica Sinica,English Series 2019年第8期35卷 1402-1418页

作者： ya Jun LI wen Dong wang School of Mathematical Sciences Dalian University of Technology

In this note we derive MHD boundary layer equations according to viscosity and resistivity coefficients. Especially, when these viscosity and resistivity coefficients are of different orders, it leads to degenerate MH... 详细信息

关键词： MHD equations degenerate boundary layer equations local well-posedness analytic spaces

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Simulating Alpine Vegetation Net Primary Productivity by Remote Sensing in Qinghai Province,China

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Journal of Mountain Science 2014年第4期11卷 967-978页

作者： WEI ya-xing wang Li-wen Center for Marine Economic and Sustainable Development Liaoning Normal University Liaoning Key Laboratory of Physical Geography and Geomatics Liaoning Normal University College of Urban and Environmental Science Liaoning Normal University

Primary productivity of ecosystem is important indicator about ecological assessment. Remote sensing technology has been used to monitor net primary productivity （NPP） of ecological system for several years. In this paper, the remotely sensed NPP simulation model of alpine vegetation in Qinghai Province of Tibet Plateau was set up based on the theory of light use efficiency. Firstly a new approach based on mixed pixels and Support Vector Machine （SVM） algorithm were used to correct simulated NPP values derived from Moderate Resolution Imaging Spectroradiometer （MODIS） data. Finally, spatial distribution and monthly variation characteristics of NPP in Qinghai Province detail. The result showed in 2006 were analyzed in that NPP of vegetation in Qinghai Province in 2006 ranged from o to 422 gC/m2/a and the average NPP was 151 gC/m2/a. NPP gradually increased from northwest to southeast. NPP of different vegetation types were obviously different. The average NPP of broad-leaved forest was the largest （314 gC/m2/a）, and sparse shrub was the smallest （101 gC/m2/a）. NPP in Qinghai Province significantly changed with seasonal variation. The accumulation of NPP was primarily in the period （from April to September） with better moist and heat conditions. In July, the average NPP of vegetation reached the maximum value （43 gC/m2）. In our model, the advantage of traditional LUE models was adopted, and our study fully considered typicalcharacteristics of alpine vegetation light use efficiency and environmental factors in the study area. Alpine vegetation is the most important ecological resource of Tibet Plateau, exactly monitoring its NPP value by remote sensing is an effective protection measure.

关键词： Net primary productivity Remotesensing Light use efficiency model Contexturalapproach Support Vector Machine

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Rational modulation of fluorophosphate cathode by anionic groups to reduce the polarization behavior for fast-charging sodium-ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 509-521页

作者： Xinyuan wang Fan Zhang Xingyu Zhou Qian wang Changyu Liu yangyang Liu Hui wang Xiaojie Liu Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education College of Chemistry & Materials Science Northwest University

Na3V2（PO4）2O2F （VP） is recognized as a promising cathode material for sodium-ion batteries due to its stable structural framework and high specific *** functional theory （DFT） and finite element simulations show that incorporating SO42-into VP decreases its band gap,lowers the migration energy barrier,and ensures a uniform Na+concentration gradient and stress distribution during charge and discharge ***,the average Na+diffusion coefficient of Na3V2（PO4）1.95（SO4）0.05O2F（VPS-1） is roughly double that of VP,leading to enhanced rate capability (80 C,75.5 mAh g-1) and cycling stability (111.0 mAh g-1capacity after 1000 cycles at 10 C current density) for ***-1 exhibits outstanding fast-charging capabilities,achieving an 80%state of charge in just 8.1 *** assembled VPS-1//SbSn/NPC full cell demonstrated stable cycling over 200 cycles at a high 5 C current,maintaining an average coulombic efficiency of 95.35%.

关键词： Anionic group modulation Polarization behavior Fast-charging Sodium-ion battery Fluorophosphate Cathode

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球结膜瓣下植入生物羊膜在难治性青光眼滤过手术中的应用

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国际眼科杂志 2008年第6期8卷 1248-1249页

作者：张娇王雅文陈宾李若溪中国辽宁省沈阳市第四人民医院眼科 110031

目的：观察难治性青光眼小梁切除术中不同位置植入生物羊膜对滤过泡及眼压的影响。方法：随机选择我院住院45例难治性青光眼患者，随机分为三组，A组为小梁切除术后球结膜下植入生物羊膜（15眼），B组为小梁切除术后巩膜瓣下植入生物羊... 详细信息

目的：观察难治性青光眼小梁切除术中不同位置植入生物羊膜对滤过泡及眼压的影响。方法：随机选择我院住院45例难治性青光眼患者，随机分为三组，A组为小梁切除术后球结膜下植入生物羊膜（15眼），B组为小梁切除术后巩膜瓣下植入生物羊膜（15眼）。C组为单纯小梁切除术（15眼）术后观察眼压，滤过泡，以及术后并发症。结果：随访6mo，A组术后眼压平均波动为11．80～21．22mmHg，B组术后眼压平均波动为12．48～21．52mmHg，C组术后眼压平均波动为10．60～28．48mmHg，术后6mo条件成功率A、B、C三组分别为93．3％，86．7％，66．7％。结论：生物羊膜能有效提高难治性青光眼滤过手术的条件成功率。球结膜下植入羊膜操作简单，条件成功率高于其他两组，同时加固了球结膜，减少薄壁滤过泡的发生。

关键词：青光眼小梁切除术羊膜

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Single Mode Simulation Calculation of Oscillating Buoy Wave Energy Converter with A Slider

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China Ocean Engineering 2020年第4期34卷 547-557页

作者： XIAO Lei YOU ya-ge wang Zhen-peng ZHANG ya-qun HUANG Shuo wang wen-sheng Guangzhou Institute of Energy Conversion Chinese Academy of SciencesGuangzhou 510640China Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development Guangzhou 510640China Southern Marine Science and Engineering Guangdong Laboratory Guangzhou 511458China Key Laboratory of Renewable Energy Chinese Academy of SciencesGuangzhou 510640China Innovation Academy of South China Sea Ecology and Environmental Engineering Chinese Academy of SciencesGuangzhou 510640China University of Chinese Academy of Sciences Beijing 100049China

This paper presents an oscillating slider wave energy device which is based on a seabed anchoring and uses eagle beak as the *** self-compiled program uses the boundary element theory based on the simple Green’s function to solve the wave forces and hydrodynamic *** the equation of motion,the oscillation of the float and the capture width ratio are obtained by the modal *** influences of the shape of the eagle beak,the angle of the slider and the wave heading on the capture ability of the device are *** to the calculation results and the wave resources in the sea area,the optimal shape of the eagle beak and external damping can be selected to maximize the wave energy capture capability.

关键词： wave energy converter(WEC) Sharp Eagle hydrodynamic calculation eagle beak

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Effect of nozzle type on fluid flow, solidification, and solute transport in mold with mold electromagnetic stirring

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Journal of Iron and Steel Research International 2022年第2期29卷 237-246页

作者： ya-dong wang Li-feng Zhang wen yang Ying Ren School of Metallurgical and Ecological Engineering University of Science and Technology Beijing(USTB)Beijing100083China State Key Lab of Metastable Materials Science and Technology School of Mechanical EngineeringYanshan UniversityQinhuangdao066004HebeiChina

The mathematical model of coupling fluid flow,heat transfer,solidification,solute transport,and the electromagnetic field of the bloom in the upper part of the strand was established with three nozzle ***,the flow field,distribution of the temperature,solidification,and macrosegregation of carbon were investigated and compared by numerical *** the case of the straight submerged entry nozzle(SEN),the molten steel flows down deep into the liquid pool,and the depth of the jet flow reaches about 1.0 m beneath the *** jetting zone is the high-temperature *** the case of two-port SEN and four-port SEN,the flow patterns and distribution of temperature in the central longitudinal section are *** jet flow impinges directly on the initially solidified shell and then it is divided into two longitudinal *** heat of molten steel is dissipated along with the longitudinal *** negative segregation band was generated near the bloom surface due to the washing effect by the rotating flow at the solidification front with three nozzle *** negative segregation deteriorates gradually with the number of ports decreasing.

关键词： Submerged entry nozzle Fluid flow Solidification Solute transport Mold electromagnetic stirring

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