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[Construction of a 17 β-estradiol sensor based on a magnetic graphene oxide/aptamer separating material]

Se pu = Chinese journal of chromatography 2025年第1期43卷 87-95页

作者： Xin-Yu Jin Le-Yuan Chen ya-Nna liu Wen-Jing Xie Han-Yong Peng State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China. University of Chinese Academy of Sciences Beijing 100049 China. School of Environment Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences Hangzhou 310024 China.

17-Estradiol (E2) is a natural steroidal estrogen essential for a variety of physiological functions in organisms. However, external E2, which is renowned for its potent biological effects, is also considered to be an endocrine-disrupting compound (EDC) capable of disturbing the normal operation of the endocrine system, even at nanogram-per-liter (ng/L) concentrations. Studies have revealed that medical and livestock wastewater can be contaminated with E2, which poses potential risks to human health. Currently, the primary method for detecting E2 relies on liquid chromatography-mass spectrometry, which is limited with regard to on-site or large-scale sample testing due to instrumental constraints. Herein, we developed a magnetic graphene oxide (MGO)/aptamer separating material. The MGO was synthesized by creating a water-in-oil microemulsion at 90 ℃, an agarose hydrogel to load the FeO nanoparticles, and layered graphene oxide (GO). In contrast to conventional methods, such as chemical co-precipitation and solvothermal approaches, this method is more time-efficient and does not require high temperature or pressure. Moreover, the use of a physical encapsulation technique for enwrapping the FeO nanoparticles and layered GO eliminates the need for chemical modification. This approach reduces the use of harmful chemicals, ensures complete loading, and results in highly efficient encapsulation. The MGO was characterized using Fourier-transform infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM), as well as dynamic light scattering (DLS) and Zeta potential analyses, which revealed that the FeO nanoparticles had been successfully loaded onto the GO to produce MGO particles mainly around 5 μm in size. Additionally, this study demonstrated that the aqueous MGO dispersion is highly stable. This substance was used to develop a fluorescent biosensor that uses a "turn-on" mechanism to rapidly and highly sensitively detect E2. MGO is capable of adsorbing a fl

关键词： 17-estradiol (E2) fluorescence biosensor magnetic graphene oxide (MGO) magnetic solid phase separation

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绿原酸对中性粒细胞功能及胞外诱捕网形成的影响及机制

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中国药理学通报 2025年第1期41卷 101-107页

作者：杨运刘娅王翠彭春红贵州中医药大学第二临床医学院贵州贵阳550025 七星关区中医妇幼集团医院贵州毕节551700 毕节市中医医院贵州毕节551700 贵州医科大学临床医学院贵州贵阳550025 贵州省人民医院呼吸与危重医学科贵州贵阳550002 国家卫健委肺脏免疫性疾病重点实验室贵州贵阳550002

目的探究绿原酸(chlorogenic acid,CGA)对中性粒细胞(neutrophil,NEU)功能及中性粒细胞胞外诱捕网(neutrophil extracellular traps,NETs)形成的影响及机制。方法从小鼠骨髓中分离并纯化NEU;将NEU与CGA共培养,通过CCK-8法筛选共培养适合的干预浓度;检测NEU迁移能力、髓过氧化物酶(MPO)以及脱颗粒弹性蛋白酶(NE)、基质金属蛋白酶9(MMP-9)的水平观察CGA对NEU功能的影响;免疫荧光法观察NETs的主要结构组蛋白H3及DNA骨架,以及检测NEU释放的游离DNA(cf-DNA)含量,观察NETs的形成情况;检测NEU产生的活性氧(ROS)水平,以确定CGA是否影响NEU释放NETs过程中的ROS的生成。结果CGA在浓度为100、200、400μmol·L^(-1)时对NEU活性有明显抑制作用(P<0.01)。与空白组(Blank)相比,CGA(100、200、400μmol·L^(-1))干预后,细胞迁移能力及MPO、NE、MMP-9分泌量增加(P<0.05),组蛋白H3及DNA骨架、cf-DNA的释放量明显增加(P<0.01),细胞产生的ROS也明显增加(P<0.01),上述作用在一定范围内具有浓度依赖性。结论CGA能够增强NEU功能及促进NETs的形成,可能与促进细胞内ROS水平增加有关。

关键词：绿原酸中性粒细胞胞外诱捕网免疫抗菌活性氧

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内蒙古农牧交错区典型黑土坡耕地土壤侵蚀空间分布特征

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水土保持研究 2025年第1期32卷 13-20页

作者：邹惠杰刘刚舒成博孙波刘亚张琼郭珍中国科学院教育部水土保持与生态环境研究中心黄土高原土壤侵蚀与旱地农业国家重点实验室中国科学院水利部水土保持研究所中国科学院大学西北农林科技大学黄土高原土壤侵蚀与旱地农业国家重点实验室四川华标测检测技术有限公司

[目的]定量了解内蒙古典型黑土区土壤侵蚀速率，探究该区域土壤侵蚀空间分布特征，阐明土壤侵蚀坡面和区域空间分布规律，以期为区域水土流失治理提供参考。[方法]采集内蒙古农牧交错区（海拉尔区、额尔古纳市和牙克石市）典型黑土坡耕... 详细信息

[目的]定量了解内蒙古典型黑土区土壤侵蚀速率，探究该区域土壤侵蚀空间分布特征，阐明土壤侵蚀坡面和区域空间分布规律，以期为区域水土流失治理提供参考。[方法]采集内蒙古农牧交错区（海拉尔区、额尔古纳市和牙克石市）典型黑土坡耕地的土壤样品，利用137Cs示踪技术计算各采样点土壤侵蚀速率，结合小波分析的方法研究土壤侵蚀在坡面的分布特征。[结果]（1） 3个坡面所有采样点137Cs含量均小于背景值（1 984.24 Bq/m2）,表现为所有坡面土壤流失。（2） 3个地区的采样坡面平均侵蚀速率表现为：海拉尔[6 533.37 t/（km2·a）]>额尔古纳[4 174.35 t/（km2·a）]>牙克石[2 765.87 t/（km2·a）]。相同坡面的3个纵向断面的平均侵蚀速率相近，同一断面侵蚀速率的变异程度较高。（3）土壤侵蚀速率沿坡长呈强弱交替的周期性变化，但周期长度并不固定。大部分纵向断面存在周期长度为80～160 m和160～200 m的大小两个周期。部分纵向断面只存在一个周期长度为100～120 m的周期。[结论]该地区土壤侵蚀在坡面尺度表现为全坡面侵蚀且在纵向呈现周期性特征，在区域尺度呈现从东到西逐渐增加趋势。

关键词：土壤侵蚀典型黑土农牧交错带小波分析

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Improving Solution of Euler Equations by a Gas-Kinetic BGK Method

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西北工业大学学报 2009年第1期27卷 1-5页

作者： liu, ya Gao, Chao liu, F. National Key Laboratory of Aerodynamic Design and Research Northwestern Polytechnical University Xi'an 710072 China Department of Mechanical and Aerospace Engineering University of California Irvine CA 92697-3975 United States

*** well known JST(Jameson-Schmidt-Turkel) scheme requires the use of a dissipation *** propose using gas-kinetic BGK(Bhatnagar-Gross-Krook) method,which is based on the more fundamental Boltzmann equation,in order to obviate the use of dissipation term and obtain,we believe,an improved *** 1 deals essentially with three things:(1) as analytical solution of molecular probability density function at the cell interface has been obtained by the Boltzmann equation with BGK model,we can compute the flux term by integrating the density function in the phase space;eqs.(8) and(11) require careful attention;(2) the integrations can be expressed as the moments of Maxwellian distribution with different limits according to the analytical solution;eqs.(9) and(10) require careful attention;(3) the discrete equation by finite volume method can be solved using the time marching *** are performed by the BGK method for the Sod′s shock tube problem and a two-dimensional shock reflection *** results are compared with those of the conventional JST scheme in Figs.1 and *** BGK method provides better resolution of shock waves and other features of the flow fields.

关键词： Euler equations Boltzmann equation gas-kinetic BGK(Bhatnagar-Gross-Krook) scheme JST(Jameson-Schmidt-Turkel) scheme shock tube shock reflection

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Reliability analysis on civil engineering project based on integrated adaptive simulation annealing and gray correlation method

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Frontiers of Structural and Civil Engineering 2016年第4期10卷 462-471页

作者： Xiao-ping BAI ya-nan liu System Engineering Research Institute School of Management Xi 'an University of Architecture & Technology Xi 'an 710055 China

Dynamic reliability is a very important issue in reliability research. The dynamic reliability analysis for the project is still in search of domestic and international research in the exploration stage. By now, dynamic reliability research mainly concentrates on the reliability assessment; the methods mainly include dynamic fault tree, extension of event sequence diagram and Monte Carlo simulation, and et al. The paper aims to research the dynamic reliability optimization. On the basis of analysis of the four quality influence factors in the construction engineering, a method based on gray correlation degree is employed to calculate the weights of factors affecting construction process quality. Then the weights are added into the reliability improvement feasible index （RIFI）. Furthermore, a novel nonlinear programming mathematic optimization model is established. In the Insight software environment, the Adaptive Simulated Annealing （ASA） algorithm is used to get a more accurate construction subsystem optimal reliability under different RIFI conditions. In addition, the relationship between construction quality and construction system reliability is analyzed, the proposed methods and detailed processing can offer a useful reference for improving the construction system quality level.

关键词： civil engineering dynamic reliability grey relational degree adaptive simulated annealing algorithm

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ABRUPT DEFLECTED SUPERCRITICAL WATER FLOW-REVISED THEORY OF SHOCK WAVE

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Journal of Hydrodynamics 2006年第1期18卷 32-39页

作者： NI Han-gen liu ya-kun State Key Laboratory of Coastal and Offshore Engineering Dalian University of Technology Dalian 116024 China

The shock wave angle and depth ratio of the abrupt deflected supercritical water flow due to deflector was investigated experimentally and theoretically. A correction coefficient of the hydro-dynamic pressure ξ was introduced to generalize the momentum equation in the perpendicular direction to the shock front. An extensive series of tests were conducted in a 1 m wide flume with the Froude number ranging from 1.70 to 8.37, the deflection angle ranging from 5° to 40° and the length of deflector ranging from 0.28 m to 1 m. A dimensionless parameter K was defined to depict the ratio of the flow height to flow thickness. Test results show that the val ue of ~, the correction coefficient of the non-hydrostatic pres- sure distribution, decreases with the increase of the value of K An empirical relationship between the value of ξ and the val ue of K was proposed. It is indicated that the relative errors of the results calculated by the revised theory is much smaller than that obtained from the Ippen theory. Finally, a simple explicit expression was suggested to calculate the shock wave height ratio in consideration of the effect of the non-hydrostatic pressure distribution.

关键词： theory of shock wave abrupt deflection of supercritical flow trajectory type energy dissipater

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MODIFIED LOG-WAKE LAWS FOR TURBULENT FLOW OF THE OUTER AND INNER REGIONS IN SMOOTH PIPES

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Journal of Hydrodynamics 2007年第2期19卷 188-194页

作者： liu ya-kun NI Han-gen State key Laboratory of Coastal and offshore Engineering Dalian University of Technology Dalian 116024 china

Based on theoretical analysis two modified log-wake laws for turbulent flow in smooth pipes are obtained, one is applicable to the outer region and other one to inner region, the new law for the outer region fits the velocity profile measured in smooth pipes by Zagarola very well and the effect of Reynolds number can be taken into consideration, the velocity profile for inner region satisfies the wall boundary conditions ,equals zero at the pipe wall and smoothly joins up with the velocity profile in outer region, the adopted eddy viscosity model is consistent with Laufer＇s, Nunner＇s and Reichardt＇s experimental data.

关键词： turbulent pipe flow eddy viscosity log-wakelaw outer and inner regions

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Computational Bioluminescence

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Chinese Journal of Chemistry 2021年第1期39卷 43-49页

作者： Yi-Qi Tang ya-Jun liu College of Chemistry Beijing Normal UniversityBeijing 100875China

Bioluminescence(BL)is an amazing natural phenomenon whose visible light is pro-duced by living *** to its high sensitivity,high selectivity and high signal-to-noise ratio,BL has been applied broadly in biotechnology and biomedical ***,for centuries,we can only receive some sporadic and static information from experimental observations,the mechanism of most BL is *** the past 14 years,we have been performing theoretical study on all kinds of bioluminescent systems,and have uncovered the mechanism and details of several *** as an example,we qualitatively introduce our theoretical study of firefly BL in this account.

关键词： Bioluminescence Reaction mechanisms Density functional calculations Ab initio calculations Molecular dynamics

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Theoretical analysis of the coupling between Feshbach states and hyperfine excited states in the creation of 23Na40K molecule

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Chinese Physics B 2020年第2期29卷 218-223页

作者： ya-Xiong liu Bo Zhao Hefei National Laboratory for Physical Sciences at Microscale and Department of Modern Physics University of Science and Technology of ChinaHefei 230026China Shanghai Branch CAS Center for Excellence and Synergetic Innovation Center in Quantum Information and Quantum PhysicsUniversity of Science and Technology of ChinaShanghai 201315China

We present an intensive study of the coupling between different Feshbach states and the hyperfine levels of the excited states in the adiabatic creation of 23Na40K ground-state *** use coupled-channel method to calculate the wave function of the Feshbach molecules,and give the short-range wave function of triplet *** energies of the hyperfine excited states and the coupling strength between the Feshbach states and the hyperfine excited states are *** results can be used to prepare a specific hyperfine level of the rovibrational ground state to study the ultracold collisions involving molecules.

关键词： Feshbach resonance stimulated Raman adiabatic passage ultracold molecule

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Perturbed magnetic field of an infinite plate with a centered crack

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Acta Mechanica Sinica 2011年第2期27卷 259-265页

作者： Fei Qin yang Zhang ya-Nan liu College of Mechanical Engineering and Applied Electronics Technology Beijing University of Technology 100124 Beijing China

Deforming a cracked magnetoelastic body in a magnetic field induces a perturbed magnetic field around the crack. The quantitative relationship between this perturbed field and the stress around the crack is crucial in developing a new generation of magnetism-based nondestructive testing technologies. In this paper, an analytical expression of the perturbed magnetic field induced by structural deforma- tion of an infinite ferromagnetic elastic plate containing a centered crack in a weak external magnetic field is obtained by using the linearized magnetoelastic theory and Fourier transform methods. The main finding is that the perturbed magnetic field intensity is proportional to the applied tensile stress, and is dominated by the displacement gradient on the boundary of the magnetoelastic solid. The tangential component of the perturbed magnetic-field intensity near the crack exhibits an antisymmetric distribution along the crack that reverses its direction sharply across its two faces, while the normal component shows a symmetric distribution along the crack with singular points at the crack tips.

关键词： Magnetoelasticity Crack Perturbed magnetic field Fourier transform method Nondestructive testing

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