检索结果-南通市图书馆

Simultaneous determination of tyrosine and dopamine in urine samples using excitation-emission matrix fluorescence coupled with second-order calibration

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2013年第3期24卷 239-242页

作者： Shan-Shan Li Hai-Long Wu ya-juan liu Hui-Wen Gu Ru-Qin Yu State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan University

A ＂green＂ and quick analytical method for complex compounds was developed for simultaneous determination of tyrosine （Tyr） and dopamine （DA） in urine samples in this paper. The three-way responsive data recorded by excitation-emission matrix fluorescence （EEM） spectrometer was analyzed using second-order calibration methods based on both parallel factor analysis （PARAFAC） and self- weighted alternating trilinear decomposition （SWATLD） algorithms. The EEM spectra of the analytes were overlapped with the background in urine samples. However the second-order advantage of both PARAFAC and SWATLD methods was exploited, even in the presence of unknown interferences and the satisfactory results can be obtained. Furthermore, the linear ranges of Tyr and DA were determined to be 0.042-6.42 p.g/mL and 0.18-4.43/ug]mL, respectively, and the accuracies of both methods were validated by the analytical figures of merit （FOM）.

关键词： Second-order calibrationPARAFACSWATLDTyrosineDopamine

Simultaneous determination of metronidazole and tinidazole in plasma by using HPLC-DAD coupled with second-order calibration

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2010年第10期21卷 1223-1226页

作者： Li Qun Ouyang Hai Long Wu ya juan liu Jian Yue Wang Yong Jie Yu Hong yan Zou Ru Qin Yu State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha 410082 China Zhoushan Municipal Center for Disease Control & Prevention Zhoushan 310062 China

A method using HPLC-DAD coupled with second-order calibration was developed to simultaneously determine metronidazole and tinidazole in plasma samples in this paper. The second-order calibration method based on APTLD （alternating penalty trilinear decomposition） algorithm was proposed to analyze the three-way HPLC-DAD data from both standard and prediction samples, which makes it possible that calibration can be performed even in the presence of unknown interferences with a simple and green chromatographic condition and short analysis time. The results showed that good recoveries were obtained although the chromatographic and spectral profiles of the analytes of interest as well as background were partially overlapped with each other in plasma samples.

关键词： HPLC-DAD Second-order calibration Alternating penalty trilinear decomposition (APTLD) Metronidazole Tinidazole Plasma

Effects of U0126 on growth and activation of mitogen-activated protein kinases in Aspergillus fumigatus

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2013年第2期126卷 220-225页

作者： MA Dong-mei JI ya-juan yaNG Fang liu Wei WAN Zhe LI Ruo-yu Department of Dermatology Peking University First HospitalResearch Center for Medical Mycology Peking University Beijing100034 China

Background Invasive aspergillosis （IA）, which is mainly caused by Aspergillus fumigatus （A. fumigatus）, is a major cause of morbidity and mortality in immunocompromised patients. Despite considerable progress in currently available antifungals the mortality still remains high in critically ill patients. U0126 which is a highly selective inhibitor of MEK1 and MEK2 in the RAF/MEK/ERK pathway in mammalian cells has been demonstrated to have an anti-proliferative role in cancer cells. The purpose of this study was to explore the role of U0126 on growth inhibition and activation of mitogen-activated protein kinases （MAPKs） in A. fumigatus. Methods Germination percentage and hyphae growth in A. fumigatus treated with U0126 were observed and compared with untreated controls. Western blotting analysis was used to detect changes in activation of SakA, MpkA and MpkB. Results U0126 inhibited germination and hyphae growth in A. fumigatus and enhanced the phosphorylation of SakA and MpkA under oxidative stress. U0126 at 10 pmol/L did not block the activation of MpkB during nitrogen starvation stress. Conclusion U0126 shows promise as an antifungal candidate and the MAPK pathway may be a possible antifungal drug target for A. fumigatus.

关键词： Aspergillus fumigatus protein kinase inhibitors mitogen-activated protein kinases

Intrathecal dexamethasone and methotrexate treatment of neoplastic meningitis from solid tumors

在线全文

学校读者我要写书评

暂无评论

Neuroimmunology and Neuroinflammation 2015年第1期2卷 162-166页

作者： Wen-Jing Lv Jun-Ying He Yue-Li Zou ya-juan liu Qin-Qin Zhang Xin liu Hui Bu Department of Neurology The Second Hospital of Hebei Medical UniversityShijiazhuang 050000HebeiChina

Aim:Neoplastic meningitis(NM)from solid tumors is an advanced malignancy with poor *** chemotherapy is a reliable treatment,and we have obtained some experiences in the treatment of NM with intrathecal dexamethasone and methotrexate(IT DXM and MTX).Methods:Retrospective study of 23 patients with NM from lung cancer(n=11),breast cancer(n=3),gastric cancer(n=1),malignant melanoma(n=1),unknown cancer(n=7)was *** these patients,eight received IT DXM and MTX treatment,and 15 patients were placed into a palliative care *** survival(OS)was compared,and the patients’characteristics,symptoms,and some laboratory examinations were analyzed to find the risk factors affecting ***:OS of IT DXM and MTX group was significantly longer than that of the palliative care group(P=0.01).The median survival(MS)of palliative care group was 7.53 weeks(5.50-9.57;n=15),and of the IT DXM and MTX group,28.63 weeks(12.50-44.75;n=8);IT DXM and MTX prolonged the OS of NM patients(regression coefficient=−2.923),with odds ratio(OR)being 0.054(0.09-0.323).Spinal nerves damage decreased the OS(regression coefficient=1.595),with OR being 4.928(1.382-17.579).Conclusion:IT DXM and MTX have prolonged the patients’MS,which could be used as a fundamental treatment of *** of induction treatment should be flexible and individualized,and induction treatment could restart when central nervous system *** with spinal nerves damage are apt to live shorter.

关键词： Clinical experiences intrathecal dexamethasone and methotrexate neoplastic meningitis

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang Xin liu Jian Zhang ya Gao Shuyue Li Cui Chang Xiang liu Guofeng yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

Double [5]carbohelicene: facile synthesis, chiroptical properties,isomerization study, and lasing application

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2025年第1期68卷 233-240页

作者： Peipei liu yantong Li Meng-Xiang Wu Hao Kang Xiao-Li Zhao Lin Xu Linlin liu Xiaodong Li Junfeng Fang Zhiwei Fang ya Cheng Hai-Bo yang Huakang Yu Xueliang Shi State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical ProcessesSchool of Chemistry and Molecular Engineering East China Normal University School of Physics and Optoelectronics South China University of Technology Institute of Polymer Optoelectronic Materials & Devices State Key Laboratory of Luminescent Materials & Devices South China University of Technology School of Physics and Electronic Science Engineering Research Center of Nanophotonics & Advanced Instrument Ministry of EducationEast China Normal University State Key Laboratory of Precision Spectroscopy School of Physics and Electronic Science East China Normal University

Double carbohelicenes have attracted considerable attention due to their aesthetic structures, distinct π-conjugation extension, inherent chirality, and intriguing optical and electronic properties. Herein, the concise de novo synthesis of a new double[5]carbohelicene 1 together with its chiroptical properties, isomerization process and lasing application is presented. 1 was synthesized by a simple protocol involving the formation of a 6,6′-bipentacene as the key intermediate. It is worth noting that1 existed as two diastereomers 1-PP/MM and 1-PM, which were successfully isolated and unambiguously confirmed by Xray crystallography. The optical resolution of the racemic 1-PP/MM was successfully achieved by chiral-phase highperformance liquid chromatography（HPLC）, and 1-PP and 1-MM were characterized by circular dichroism. Interestingly, it was found that 1-PP/MM could be completely isomerized to 1-PM upon heating, and the detailed thermodynamics and kinetics of the isomerization process were systematically investigated. 1-PM exhibited deep-red luminescence with emission maximum at 723 nm and fluorescence quantum yield as high as 32.95%. Consequently, we can showcase the lasing application of 1-PM by doping with epoxy resin to assemble a bottle microlaser spontaneously, which the lasing wavelength is 796.6 nm at a threshold of 0.8 m J cm-2. We believe that our studies, including the facile synthesis methodology, detailed isomerization studies, and lasing application, will shed some light on the design and synthesis of novel carbohelical systems with more functions.

关键词： double carbohelicene optical resolution organic laser isomerization X-ray diffraction

Salsolinol as an RNA m~6A methylation inducer mediates dopaminergic neuronal death by regulating yaP1 and autophagy

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第3期20卷 887-899页

作者： Jianan Wang Yuanyuan Ran Zihan Li Tianyuan Zhao Fangfang Zhang juan Wang Zongjian liu Xuechai Chen Beijing International Science and Technology Cooperation Base for Antiviral Drugs College of Chemistry and LifeBeijing University of TechnologyBeijingChina Department of Rehabilitation Beijing Rehabilitation HospitalCapital Medical UniversityBeijingChina

Salsolinol(1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline,Sal)is a catechol isoquinoline that causes neurotoxicity and shares structural similarity with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,an environmental toxin that causes Parkinson's ***,the mechanism by which Sal mediates dopaminergic neuronal death remains *** this study,we found that Sal significantly enhanced the global level of N~6-methyladenosine(m~6A)RNA methylation in PC12 cells,mainly by inducing the downregulation of the expression of m~6A demethylases fat mass and obesity-associated protein(FTO)and alk B homolog 5(ALKBH5).RNA sequencing analysis showed that Sal downregulated the Hippo signaling *** m~6A reader YTH domain-containing family protein 2(YTHDF2)promoted the degradation of m~6A-containing Yes-associated protein 1(yaP1)mRNA,which is a downstream key effector in the Hippo signaling ***,downregulation of yaP1 promoted autophagy,indicating that the mutual regulation between yaP1 and autophagy can lead to *** findings reveal the role of Sal on m~6A RNA methylation and suggest that Sal may act as an RNA methylation inducer mediating dopaminergic neuronal death through yaP1 and *** results provide greater insights into the neurotoxic effects of catechol isoquinolines compared with other studies and may be a reference for assessing the involvement of RNA methylation in the pathogenesis of Parkinson's disease.

关键词： ALKBH5 autophagy FTO Hippo pathway m~6A Parkinson's disease RNA methylation salsolinol yaP1 YTHDF2

The Selection of the Dempster’s Rule Based on Evidence Trap Problem in D-S Theory

在线全文

学校读者我要写书评

暂无评论

The Selection of the Dempster’s Rule Based on Evidence Trap...

Enhanced rate capability and cycle stability of Ti2C MXene for sodium storage through an aniline molecules welding strategy

第24届中国控制与决策会议

作者： Haixin Zhang, Bingyi Kang, Daijun Wei, ya Li, juan liu and Yong Deng the School of Computer and Information Sciences, Southwest University,Chongqing, 400715, China

Dempster’s rule may not handle the conflicting belief structures in several situations. The Dempster’s combination rule and its alternatives have been under the microscope. This paper focus on the conflicting belief structure problem and comes up with an evidence trap problem. A method of determining whether to select the Dempster’s rule of combination or not when there is an evidence trap is proposed. The method deploys the conflict coefficient and the distance between belief structures as a two-dimensional measure with two thresholds settled. Numeric examples show the efficiency of the proposed method.

关键词： Evidence trap D-S theory Conflict Alternative combination rule

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Tungsten 2025年第1期7卷 161-171页

作者： Hao Chen ya-Qin Wang Dong-Ting Zhang Bei Zhao Min-Peng Li Chen-yang Li Tian-Peng Xu Mao-Cheng liu State Key Laboratory of Advanced Processing and Recycling of Non-Ferrous Metals Lanzhou University of Technology School of Materials Science and Engineering Lanzhou University of Technology School of Computer and Communication Technology Lanzhou University of Technology

MXenes obtained significant attention in the field of energy storage devices due to their characteristic layered structure,modifiable surface functional groups,large electrochemically active surface,and regulable interlayer ***,the self-restacking and sluggish ions diffusion kinetics performance of MXenes during the alkali metal ions insertion/extraction process severely impedes their cycle stability and rate *** paper proposes an aniline molecule welding strategy for welding p-phenylenediamine（PPDA） into the interlayers of Ti2C through a dehydration condensation *** welded PPDA molecules can contribute pillar effect to the layered structure of *** pillar effect effectively maintains the structural stability during the sodium ions insertion/extraction process and effectively expands the interlayer spacing of Ti2C from 1.16 to 1.38 nm,thereby enhancing ions diffusion kinetics performance and improving the long-term cycle *** Ti2C-PPDA demonstrates outstanding Na+storage capability,exhibiting a specific capacity of 100.2 mAh·g-1at a current density of 0.1 A·g-1over 960 cycles and delivering a remarkable rate capability 81.2 mAh·g-1at a current density of 5 A·*** study demonstrates that expanding interlayer spacing is a promising strategy to enhance the Na+storage capacity and improve long-term cycling stability,which provides significant guidance for the design of two-dimensional Na+storage materials with high-rate capability and cycle stability.

关键词： Diffusion kinetics Expanding interlayer spacing Rate capability Sodium ions storage Ti2C