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Effects of antibacterial compounds produced by Saccharomyces cerevisiae in Koumiss on pathogenic Escherichia coli O8 and its cell surface characteristics

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Journal of Integrative Agriculture 2017年第3期16卷 742-748页

作者： CHEN Yu-jie WANG Chun-jie HOU Wen-qian WANG Xiao-shuo GALI Bing-ga HUASAI si-mu-jide yang si-qin WU A-qi-ma ZHAO Yu-fei WU Ying-ga CHEN Ao-ri-ge-le Vocational and Technical College Inner Mongolia Agricultural University Baotou 014109 P.R. China College of Animal Science Inner Mongolia Agricultural university Hohhot 010018 P.R. China College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot 010018 P.R. China

The effects of antibacterial compounds produced by Saccharomyces cerevisiae in Koumiss on pathogenic Escherichia coli O8 and its cell surface characteristics were *** isolated from Koumiss produced antibacterial compounds which were active against pathogenic *** O8 as determined by the Oxford cup *** aqueous phases from *** at p H=2.0（S2）and pH=8.0（S8）were extracted and tested,*** organic acids of S2 and S8 were determined by high performance liquid chromatography（HPLC）,and the concentrations of killer toxins were determined by enhanced bicinchoninic acid（BCA）Protein Assay *** minimum inhibition concentration（MIC）and the minimum bactericidal concentration（MBC）of S2 and S8 on *** O8 were determined by the broth microdilution *** effects of S2 and S8 on the growth curve of *** O8 were determined by turbidimetry,and the hydrophobicities of *** O8 cell surface were determined using the microbial adhesion to solvents method,the permeation of *** O8 cell membrane were determined by the o-nitrophenyl-β-D-galactoside（ONPG）*** phases at pH 2.0 and 8.0had larger inhibition zones and then S2 and S8 were obtained by *** main component in S2 was citric acid and it was propanoic acid in *** organic acids and killer toxins were also *** the MICs of S2 and S8 on *** O8 were 0.025 g m L^-1,the MBCs were 0.100 and 0.200 g m L^-1,*** normal growth curve of *** O8was S-shaped,however,it changed after addition of S2 and *** O8 was the basic character,and had a relatively hydrophilic *** hydrophobicity of *** O8 cell surface and the permeation of *** O8 cell membrane were increased after adding S2 and *** present study showed that S2 and S8 inhibit the growth of pathogenic *** O8and influence its cell surface characteristics.

关键词： antibacterial compound Saccharomyces cerevisiae Escherichia coil hydrophobicity permeation

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Delivery room resuscitation intensity and associated neonatal outcomes of 24^(+0)-31^(+6) weeks'preterm infants in China:a retrospective cross-sectional study

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World Journal of Pediatrics 2024年第1期20卷 64-72页

作者： si-Lu Wang Chun Chen Xin-Yue Gu Zhao-qing Yin Le Su si-Yuan Jiang Yun Cao Li-Zhong Du Jian-Hua Sun Jiang-qin Liu Chuan-Zhong yang Department of Neonatology Shanghai Key Laboratory of Maternal Fetal MedicineShanghai Institute of Maternal-Fetal Medicine and Gynecologic OncologyShanghai First Maternity and Infant HospitalSchool of MedicineTongji UniversityNo.2699Gaoke Western RoadPudong DistrictShanghai 201204China Department of Neonatology Affiliated Shenzhen Maternity and Child Healthcare HospitalSouthern Medical UniversityNo.2004Hongli RoadFutian DistrictShenzhen 518028China NHC Key Laboratory of Neonatal Diseases Fudan UniversityChildren's Hospital of Fudan UniversityShanghaiChina Department of Neonatology People's Hospital of DehongKunming Medical UniversityDehongChina Department of Neonatology Children's Hospital of Fudan UniversityShanghaiChina Department of Neonatology Children's HospitalZhejiang University School of MedicineHangzhouChina Department of Neonatology Shanghai Children's Medical CenterSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina

Background The aim of this study was to review current delivery room(DR)resuscitation intensity in Chinese tertiary neonatal intensive care units and to investigate the association between DR resuscitation intensity and short-term outcomes in preterm infants born at 24+0_31+6 weeks gestation age(GA).Methods This was a retrospective cross-sectional *** source population was infants born at 24+0_31+6 weeks'GA who were enrolled in the Chinese Neonatal Network 2019 *** infants were categorized into five groups:(1)regular care;(2)oxygen supplementation and/or continuous positive airway pressure(O2/CPAP);(3)mask ventilation;(4)endotracheal intubation;and(5)cardiopulmonary resuscitation(CPR).The association between DR resuscitation and shortterm outcomes was evaluated by inverse propensity score-weighted logistic *** Of 7939 infants included in this cohort,2419(30.5%)received regular care,1994(25.1%)received O,/CPAP,1436(18.1%)received mask ventilation,1769(22.3%)received endotracheal intubation,and 321(4.0%)received CPR in the *** maternal age and maternal hypertension correlated with a higher need for resuscitation,and antenatal steroid use tended to be associated with a lower need for resuscitation(Psignificantly with increasing amounts of resuscitation in DR after adjusting for perinatal *** strategies vary widely between centers,with over 50%of preterm infants in eight centers requiring higher intensity *** Increased intensity of DR interventions was associated with increased mortality and morbidities in very preterm infants in *** is wide variation in resuscitative approaches across delivery centers,and ongoing quality improvement to standardize resuscitation practices is needed.

关键词： Cardiopulmonary resuscitation Delivery room Endotracheal intubation Neonatal resuscitation Preterm

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输卵管妊娠术后持续性异位妊娠的危险因素分析

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解放军医学杂志 2022年第2期47卷 157-161页

作者：于津杨思勤上海交通大学医学院附属国际和平妇幼保健院妇产科/上海市胚胎源性疾病重点实验室/上海市临床重点专科建设-“强主体”妇产科上海200030

目的探讨输卵管妊娠患者术后发生持续性异位妊娠(PEP)的危险因素。方法选取2000年1月-2020年12月在上海交通大学医学院附属国际和平妇幼保健院接受输卵管妊娠手术且术后发生PEP的38例患者为PEP组,同时选择同期行相同手术且手术成功、术... 详细信息

目的探讨输卵管妊娠患者术后发生持续性异位妊娠(PEP)的危险因素。方法选取2000年1月-2020年12月在上海交通大学医学院附属国际和平妇幼保健院接受输卵管妊娠手术且术后发生PEP的38例患者为PEP组,同时选择同期行相同手术且手术成功、术后恢复良好的152例患者为对照组。回顾性分析两组患者的临床资料,记录并分析比较所有患者的年龄、孕次、体重指数(BMI)、停经时间、既往输卵管手术史、术后血清β-人绒毛膜促性腺激素(β-hCG)下降率、输卵管妊娠部位、异位妊娠病灶情况、术中盆腔粘连情况等。分析两组的临床基线资料,采用χ;检验进行输卵管妊娠术后PEP危险因素的单因素分析,采用Poisson回归进行多因素分析,筛选输卵管妊娠术后PEP的独立危险因素,并分析输卵管妊娠术后PEP的治疗方法等。结果 PEP组的年龄、孕次、BMI、停经时间等与对照组比较差异均无统计学意义(P>0.05)。单因素分析结果显示,输卵管妊娠手术后PEP的发生与急诊/择期手术、盆腔粘连、输卵管妊娠病灶破裂/流产有关,差异有统计学意义(P<0.05),而与手术方式、输卵管妊娠部位、输卵管妊娠病灶大小等无明显相关。Poisson回归分析结果显示,盆腔粘连、输卵管妊娠病灶破裂/流产是输卵管妊娠术后发生PEP的独立危险因素。输卵管妊娠患者手术后发生PEP的患者95%可经药物治疗成功。结论盆腔粘连、输卵管妊娠病灶破裂/流产是输卵管妊娠术后发生PEP的独立危险因素。

关键词：输卵管妊娠术持续性异位妊娠危险因素

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Epitaxial quasi-2D/3D hybrid perovskite heterojunctions for photodetector with enhanced detectivity and stability

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Nano Research 2024年第7期17卷 6594-6602页

作者： Yifu Chen Lin Zhang Xinxin Peng Weiran qin Shiqing Li Yingwei Wang Zhihui Chen si Xiao Bin yang Biao Liu Junliang yang Han Huang Yun Lin Jun He Liming Ding Yongbo Yuan Hunan Key Laboratory of Super Microstructure and Ultrafast Process School of PhysicsCentral South UniversityChangsha 410083China Hunan Key Laboratory of Nanophotonics and Devices School of PhysicsCentral South UniversityChangsha 410083China College of Materials Science and Engineering Hunan UniversityChangsha 410082China Department of Photoelectric Information School of PhysicsCentral South UniversityChangsha 410083China Center for Excellence in Nanoscience Key Laboratory of Nanosystem and Hierarchical FabricationNational Center for Nanoscience and TechnologyChinese Academy of ScienceBeijing 100190China

Although numerous metal halide perovskite materials have been investigated in the field of optoelectronic,the development of perovskite heterojunctions with exotic structures is still ***,we report the epitaxial growth of quasi-two-dimensional(Q-2D)perovskites on methylammonium lead iodide(MAPbI_(3))single crystals to form perovskite heterojunctions with interfacial *** MAPbI_(3)adjacent to epitaxial Q-2D perovskite shows blue shifted photoluminescence with shortened lifetime,which becomes significant with the reduced layer number of the Q-2D *** findings suggest the presence of an interfacial strain gradient leading to enhanced photocarrier ***,compared to the MAPbI_(3)single crystal detector,the BA_(2)MAPb_(2)I_(7)/MAPbI_(3)(BA:n-butylamine)heterojunction-based photodetector demonstrates a bandpass detecting property and exhibits 5 times enhanced external quantum efficiency and 83 times enhanced specific detectivity(D*=3.26×10^(11)Jones).Remarkably,the unencapsulated BA_(2)MAPb_(2)I_(7)/MAPbI_(3)heterojunction is stable in ambient condition for>300 *** Q-2D/3D heterojunction shows suppressed ion inter-diffusion due to the presence of Q-2D phase.

关键词： epitaxial growth interfacial strain quasi-two-dimensional/three-dimensional(Q-2D/3D)heterojunctions ion inter-diffusion

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Measurement of the high energyγ-rays from heavy ion reactions usingČerenkov detector

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Nuclear Science and Techniques 2024年第2期35卷 123-130页

作者： Da-Wei si Yan Zhou Sheng Xiao Zhi qin Dong Guo Yu-Hao qin Yi-Jie Wang Bo-Yuan Zhang Bai-Ting Tian Zhi-Gang Xiao Department of Physics Tsinghua UniversityBeijing 100084China

The energetic bremsstrahlung photons up to 100 MeV produced in heavy ion collisions can be used as a sensitive probe for short-range correlation in atomic nuclei. The energy of the γ-rays can be measured by collecting the Čerenkov light in the medium induced by the fast electrons generated in the Compton scattering or electromagnetic shower of the incident γray. Two types of detectors based on pure water and lead glass as sensitive materials were designed for this purpose. The γresponse and optical photon propagation in the detectors were simulated based on electromagnetic and optical processes in Geant4. The inherent energy resolutions of 0.022(4) + 0.51(2)∕E^(1/2)_(γ) for water and 0.0026(3) + 0.446(3)∕E^(1/2)_(γ) for lead glass were obtained. The geometry sizes of the lead glass and water were optimized to 30 cm × 30 cm × 30 cm and 60 cm × 60 cm ×120 cm, respectively, to detect high-energy γ-rays at 160 MeV. The Hough transform method was applied to reconstruct the direction of the incident γ-rays, providing the ability to experimentally distinguish the high-energy γ-rays produced in the reactions on the target from random background cosmic-ray muons.

关键词： Bremsstrahlungγ-rays Čerenkov Geant4 Energy resolution Direction reconstruction Hough transform

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Anion exchange membranes with a semi-interpenetrating polymer network using 1,6-dibromohexane as bifunctional crosslinker

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Chinese Journal of Chemical Engineering 2024年第8期72卷 199-208页

作者： Aijie Li Zhanliang Wang Zhihao si Lu Lu Peipei Huang Jinhong Liu Songyuan Yao Peiyong qin Xinmiao Zhang National Energy R&D Center for Biorefinery College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijing 100029China Paris Curie Engineer School Beijing University of Chemical TechnologyBeijing 100029China Environmental Protection Research Institute Beijing Research Institute of Chemical IndustryBeijing 100000China

An anion exchange membrane(AEM)is generally expected to possess high ion exchange capacity(IEC),low water uptake(WU),and high mechanical strength when applied to electrodialysis *** different types of AEMs,semi-interpenetrating polymer networks(siPNs)have been suggested for their structural superiorities,i.e.,the tunable local density of ion exchange groups for IEC and the restrained leaching of hygroscopic groups by insolubility for ***,the conventional siPN AEMs still struggle to balances IEC,WU,and mechanical strength simultaneously,due to the lack of the compact crosslinking *** this work,we proposed a novel siPN structure of polyvinylidene difluoride/polyvinylimidazole/1,6-dibromohexane(PVDF/PVIm/DBH).On the one hand,DBH with two cationic groups of imidazole groups are introduced to enhance the ion conductivity,which is different from the conventional monofunctional modifier with only one cationic *** the other hand,DBH has the ability to bridge with PVIm,where the mechanical strength of the resulting AEM is increased by the increase of crosslinking *** show that a low WU of 38.1%to 62.6%,high IEC of 2.12—2.22 mmol·g^(-1),and excellent tensile strength of 3.54—12.35 MPa for PVDF/PVIm/DBH membrane are *** work opens a new avenue for achieving the high-quality AEMs.

关键词： Anion exchange membrane Polyvinylidene difluoride Electrodialysis Semi-interpenetrating polymer networks

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Aging hallmarks of the primate ovary revealed by spatiotemporal transcriptomics

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Protein & Cell 2024年第5期15卷 364-384页

作者： Huifen Lu Ying Jing Chen Zhang Shuai Ma Weiqi Zhang Daoyuan Huang Bin Zhang Yuesheng Zuo Yingying qin Guang-Hui Liu yang Yu Jing Qu si Wang Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China Institute for Stem cell and Regeneration CASBeijing 100101China China National Center for Bioinformation Beijing 100101China The Fifth People’s Hospital of Chongqing Chongqing 400062China sino-Danish College University of Chinese Academy of SciencesBeijing 101408China sino-Danish Center for Education and Research Beijing 101408China Department of Obstetrics and Gynecology Center for Reproductive MedicinePeking UniversityThird HospitalBeijing 100191China Clinical Stem Cell Research Center Peking UniversityThird HospitalBeijing 100191China Center for Reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education National Research Center for Assisted Reproductive Technology and Reproductive GeneticsShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthJinan 250012China Key Laboratory of Organ Regeneration and Reconstruction Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Biomarker Consortium Beijing 100101China

The ovary is indispensable for female reproduction,and its age-dependent functional decline is the primary cause of ***,the molecular basis of ovarian aging in higher vertebrates remains poorly ***,we apply spatiotemporal transcriptomics to benchmark architecture organization as well as cellular and molecular determinants in young primate ovaries and compare these to aged primate *** a global view,somatic cells within the non-follicle region undergo more pronounced transcriptional fluctuation relative to those in the follicle region,likely constituting a hostile microenvironment that facilitates ovarian ***,we uncovered that inflammation,the senescent-associated secretory phenotype,senescence,and fibrosis are the likely primary contributors to ovarian aging(PCOA).Of note,we identified spatial co-localization between a PCOA-featured spot and an unappreciated MT2(Metallothionein 2)highly expressing spot(MT2^(high))characterized by high levels of inflammation,potentially serving as an aging hotspot in the primate ***,with advanced age,a subpopulation of MT2^(high)accumulates,likely disseminating and amplifying the senescent signal *** study establishes the first primate spatiotemporal transcriptomic atlas,advancing our understanding of mechanistic determinants underpinning primate ovarian aging and unraveling potential biomarkers and therapeutic targets for aging and age-associated human ovarian disorders.

关键词： spatial transcriptome primate ovary aging senescence inflammation

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Global status and trends of metabolomics in diabetes: A literature visualization knowledge graph study

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World Journal of Diabetes 2024年第5期15卷 1021-1044页

作者： Hong Li Liu Li Qiu-qing Huang si-Yao yang Jun-Ju Zou Fan Xiao qin Xiang Xiu Liu Rong Yu College of Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina College of International Education Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Science and Technology Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Hunan Key Laboratory of TCM Prescription and Syndromes Translational Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina College of Graduate Hunan University of Chinese MedicineHunan ChangshaHunan ProvinceChina

BACKGROUND Diabetes is a metabolic disease characterized by hyperglycemia,which has increased the global medical burden and is also the main cause of death in most *** To understand the knowledge structure of global development status,research focus,and future trend of the relationship between diabetes and metabolomics in the past 20 *** The articles about the relationship between diabetes and metabolomics in the Web of Science Core Collection were retrieved from 2002 to October 23,2023,and the relevant information was analyzed using CiteSpace6.2.2R(CiteSpace),VOSviewer6.1.18(VOSviewer),and Bibliometrix software under R *** A total of 3123 publications were included from 2002 to *** the past two decades,the number of publications and citations in this field has continued to *** United States,China,Germany,the United Kingdom,and other relevant funds,institutions,and authors have significantly contributed to this *** Reports and PLoS One are the journals with the most publications and the most *** keyword co-occurrence and cluster analysis,the closely related keywords are"insulin resistance","risk","obesity","oxidative stress","metabolomics","metabolites"and"biomarkers".Keyword clustering included cardiovascular disease,gut microbiota,metabonomics,diabetic nephropathy,molecular docking,gestational diabetes mellitus,oxidative stress,and insulin *** detection analysis of keyword depicted that"Gene","microbiota","validation","kidney disease","antioxidant activity","untargeted metabolomics","management",and"accumulation"are knowledge frontiers in recent *** The relationship between metabolomics and diabetes is receiving extensive *** nephropathy,diabetic cardiovascular disease,and kidney disease are key diseases for future research in this *** microbiota,molecular docking,and untargeted metabolomics are key research directions in the *** a

关键词： Diabetes Metabolomics Bibliometric CiteSpace VOSviewer

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Study on the mechanism of near-field underwater explosion on supported thin plates with prefabricated holes

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Acta Mechanica sinica 2024年第8期40卷 32-44页

作者： si Chen Jian qin Xiangyao Meng Zhichao Lai Yanbo Wen Ruiyuan Huang School of Mechatronical Engineering Beijing Institute of TechnologyBeijing100081China Naval Academy Beijing100161China College of Civil Engineering Fuzhou UniversityFuzhou350116China

In this study,underwater explosion tests with 2.5 g trinitrotoluene explosive under different fixed plates with prefabricated holes were *** experimental results showed that the air inflow from the prefabricated hole caused the bubble to collapse earlier with an increase in the hole *** addition,the deformation mode of the thin plate transitioned from“convex”to“concave”(up to down).Next,the coupled Eulerian-Lagrangian method was used to perform the corresponding numerical *** accuracy of the numerical simulation method was verified through a comparison with the experimental *** addition,a series of numerical simulations were conducted with different prefabricated-hole diameters,blast distances,and prefabricated-hole *** results showed that the bubble-pulsating water jet substantially influenced the deformation of the thin plate when the diameter of the prefabricated hole was within the theoretical maximum bubble *** the blast distance was within the theoretical maximum bubble radius,the thin plate was subjected to only a single bubble pulsation owing to the air inflow from the prefabricated hole.

关键词： Underwater explosion Hole High-speed camera Bubble pulsation Fixed plate

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Ultrasound blood flow characteristics changes in fetal umbilical artery thrombosis:A retrospective analysis

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World Journal of Clinical Cases 2024年第2期12卷 240-248页

作者： si-Jie Hong Li-Wei Hong Xiao-qin He Xiao-Hong Zhong Department of Ultrasound Women and Children's HospitalSchool of MedicineXiamen UniversityXiamen 361000Fujian ProvinceChina Ministry of Science and Education Women and Children's HospitalSchool of MedicineXiamen UniversityXiamen 361000Fujian ProvinceChina

BACKGROUND Umbilical artery thrombosis(UAT)is extremely uncommon and leads to adverse perinatal *** of blood in pregnant women is suspected to be an important risk for *** is an effective way to detect *** mother can monitor her own fetal health using ultrasound,which enables her to take preventative action in case of *** To investigate ultrasonic blood signal after UAT in the umbilical artery,and evaluate the relationship between hypercoagulability and *** We described a case of a newly formed UAT with markedly altered ultrasonic indices of umbilical artery blood flow,and retrospectively studied it with 18 UAT patients confirmed by histopathology from October 2019 and March 2023 in Xiamen Women and Children's ***’information was collected from medical archives,including maternal clinical data,neonatal outcomes,pathological findings and ultrasonic indices of umbilical artery blood flow,such as systolic-diastolic duration ratio(S/D),resistance index(RI),pulsatility index(PI)and peak systolic velocity(PSV).Ultrasound and coagulation indices were analyzed with matched samples t-test and Wilcoxon rank sum test using the statistical packages in R(version 4.2.1)including car(version 3.1-0)and stats(version 4.2.1),and visualized by ggplot2 package(version 3.3.6).RESULTS A patient with normal findings in second and third-trimester routine ultrasound scan developed UAT with severe changes in ultrasonic indices of umbilical artery blood flow(within 2.5th of reference ranges)in a short period of *** analysis of umbilical artery blood flow ultrasound indices for 19 patients with UAT showed that the decrease in S/D,RI,and PI and increase of PSV during the disease process was greater than that of *** 18 patients delivered in our hospital showed characteristic manifestations of UAT on histological examination after delivery,most of which(16/18)showed umbilical cord abnormalities,with 1

关键词： Umbilical artery thrombosis Obstetric ultrasonography Peak systolic velocity Hypercoagulation Umbilical cord abnormalities Fetus

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