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Two-photon live imaging of direct glia-to-neuron conversion in the mouse cortex

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Neural Regeneration Research 2024年第8期19卷 1781-1788页

作者： Zongqin Xiang Shu He Rongjie Chen Shanggong Liu Minhui Liu Liang Xu Jiajun Zheng Zhouquan Jiang Long Ma ying Sun yongpeng qin yi Chen Wen Li Xiangyu Wang Gong Chen Wenliang Lei Guangdong-Hong Kong-Macao Institute of CNS Regeneration(GHMICR) Jinan UniversityGuangzhouGuangdong ProvinceChina Department of Neurosurgery The First Affiliated HospitalJinan UniversityGuangzhouGuangdong ProvinceChina Laboratory for Neuroimmunology in Health and Diseases Guangzhou First People’s HospitalSchool of MedicineSouth China University of TechnologyGuangzhouGuangdong ProvinceChina VIB-KU Leuven Center for Brain&Disease Research LeuvenFlemish RegionBelgium

Over the past decade,a growing number of studies have reported transcription factor-based in situ reprogramming that can directly conve rt endogenous glial cells into functional neurons as an alternative approach for n euro regeneration in the adult mammalian central ne rvous *** r,many questions remain regarding how a terminally differentiated glial cell can transform into a delicate neuron that forms part of the intricate brain *** addition,concerns have recently been raised around the absence of astrocyte-to-neuron conversion in astrocytic lineage-tra cing *** this study,we employed repetitive two-photon imaging to continuously capture the in situ astrocyte-to-neuron conversion process following ecto pic expression of the neural transcription factor NeuroD1 in both prolife rating reactive astrocytes and lineage-tra ced astrocytes in the mouse ***-lapse imaging over several wee ks revealed the ste p-by-step transition from a typical astrocyte with numero us short,tapered branches to a typical neuro n with a few long neurites and dynamic growth cones that actively explored the local *** addition,these lineage-converting cells were able to migrate ra dially or to ngentially to relocate to suitable ***,two-photon Ca2+imaging and patch-clamp recordings confirmed that the newly generated neuro ns exhibited synchronous calcium signals,repetitive action potentials,and spontaneous synaptic responses,suggesting that they had made functional synaptic connections within local neural *** conclusion,we directly visualized the step-by-step lineage conversion process from astrocytes to functional neurons in vivo and unambiguously demonstrated that adult mammalian brains are highly plastic with respect to their potential for neuro regeneration and neural circuit reconstruction.

关键词： astrocyte-to-neuron conversion Ca2+imaging direct lineage conversion glia astrocyte in vivo reprogramming lineage-tracing mice NeuroD1 neuron two-photon imaging

Reduced mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor contributes to neurodegeneration in a model of spinal and bulbar muscular atrophy pathology

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Neural Regeneration Research 2025年第9期20卷 2655-2666页

作者： yiyang qin Wenzhen Zhu Tingting Guo yiran Zhang Tingting Xing Peng yin Shihua Li Xiao-Jiang Li Su yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong–HongKong–Macao Institute of CNS RegenerationJinan UniversityGuangzhouGuangdong ProvinceChina

Spinal and bulbar muscular atrophy is a neurodegenerative disease caused by extended CAG trinucleotide repeats in the androgen receptor gene,which encodes a ligand-dependent transcription facto *** mutant androgen receptor protein,characterized by polyglutamine expansion,is prone to misfolding and forms aggregates in both the nucleus and cytoplasm in the brain in spinal and bulbar muscular atrophy *** aggregates alter protein-protein interactions and compromise transcriptional *** this study,we reported that in both cultured N2a cells and mouse brain,mutant androgen receptor with polyglutamine expansion causes reduced expression of mesencephalic astrocyte-de rived neurotrophic *** n of mesencephalic astrocyte-derived neurotrophic factor amelio rated the neurotoxicity of mutant androgen receptor through the inhibition of mutant androgen receptor *** down endogenous mesencephalic astrocyte-derived neurotrophic factor in the mouse brain exacerbated neuronal damage and mutant androgen receptor *** findings suggest that inhibition of mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor is a potential mechanism underlying neurodegeneration in spinal and bulbar muscular atrophy.

关键词： androgen receptor mesencephalic astrocyte-derived neurotrophic factor mouse model neurodegeneration neuronal loss neurotrophic factor polyglutamine disease protein misfolding spinal and bulbar muscular atrophy transcription factor

MEIC-global-CO_(2):A new global CO_(2)emission inventory with highly-resolved source category and sub-country information

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Science China Earth Sciences 2024年第2期67卷 450-465页

作者： Ruochong XU Dan TONG qingyang XIAO Xinying qin Cuihong CHEN Liu yAN Jing CHENG Can CUI Hanwen HU Wenyu LIU Xizhe yAN Huaxuan WANG Xiaodong LIU Guannan GENG yu LEI Dabo GUAN Kebin HE Qiang ZHANG Department of Earth System Science Ministry of Education Key Laboratory for Earth System ModelingInstitute for Global Change StudiesTsinghua UniversityBeijing100084China State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing100084China Chinese Academy of Environmental Planning Beijing100041China Institute for Carbon Neutrality Tsinghua UniversityBeijing100084China

CO_(2)emission inventory provides fundamental data for climate research and emission ***,most global CO_(2)emission inventories were developed with energy statistics from International Energy Agency(IEA)and were available at country level with limited source ***,as the first step toward a high-resolution and dynamic updated global CO_(2)emission database,we developed a data-driven approach to construct seamless and highly-resolved energy consumption data cubes for 208 countries/territories,797 sub-country administrative divisions in 29 countries,42 fuel types,and 52 sectors,with the fusion of activity data from 24 international statistics and 65 regional/local *** CO_(2)emissions from fossil fuel combustion and cement production in 1970–2021 were then estimated with highly-resolved source category(1,484 of total)and sub-country information(797 of total).Specifically,73%of global CO_(2)emissions in 2021 were estimated with sub-country information,providing considerably improved spatial resolution for global CO_(2)emission *** the support of detailed information,the dynamics of global CO_(2)emissions across sectors and fuel types were presented,representing the evolution of global economy and progress of climate *** differences of sectoral contribution were found across sub-country administrative divisions within a given country,revealing the uneven distribution of energy and economic structure among different *** estimates were generally consistent with existing databases at aggregated level for global total or large emitters,while large discrepancies were observed for middle and small *** database,named the Multiresolution Emission Inventory model for Climate and air pollution research(MEIC)is publicly available through http://*** with highly-resolved information and timely update,which provides an independent carbon emission accounting data source for climate rese

关键词： CO_(2)emissions Data-driven approach Highly-resolved source category Sub-country information

Tuna dark muscle peptides modulate host iron metabolism in the intestine through the gut microbiota and ameliorate iron deficiency anemia

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Food Science and Human Wellness 2024年

作者： Jiaojiao Han Jiayi Xiong Zhixiang Bai yang qin Chenyang Lu Jun Zhou ye Li Tinghong Ming Zhen Zhang Xiurong Su State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products， Ningbo University School of Marine Science， Ningbo University

Iron deficiency anemia (IDA) is a nutritional deficiency disease with a high incidence rate worldwide. Bioactive peptides are safe and effective， have multiple functions and can serve as potential candidates for alleviating IDA. In this study， the anti-anemia effects of tuna dark muscle peptides were explored in a diet-induced IDA mouse model. The results showed that tuna dark muscle peptides alleviated the IDA phenotype， oxidative stress and iron metabolism. In addition， tuna dark muscle peptides reversed gut microbiota dysbiosis in IDA mice. Furthermore， the transplanted fecal microbiota from tuna dark muscle peptide-treated mice also alleviated IDA symptoms and regulated iron metabolism and the gut microbiota， indicating that the anti-anemic effects were at least partially mediated by the gut microbiota. Thus， we identified a new and safe prebiotic material to alleviate IDA and provided ideas for the development of peptides. At the same time， these data also provided a theoretical basis for fecal microbiota transplantation (FMT) to alleviate IDA.

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Immune mediated support of metastasis:Implication for bone invasion

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Cancer Communications 2024年第9期44卷 967-991页

作者： Zengfeng Xin Luying qin yang Tang Siyu Guo Fangfang Li yuan Fang Gege Li yihan yao Binbin Zheng Bicheng Zhang Dang Wu Jie Xiao Chao Ni Qichun Wei Ting Zhang Department of Orthopedic Surgery Second Affiliated HospitalZhejiang University School of MedicineZhejiang UniversityHangzhouZhejiangP.R.China Cancer Institute(Key Laboratory of Cancer Prevention and Intervention National Ministry of Education)Second Affiliated HospitalZhejiang University School of MedicineZhejiang UniversityHangzhouZhejiangP.R.China Department of Radiation Oncology Second Affiliated HospitalZhejiang University School of MedicineZhejiang UniversityHangzhouZhejiangP.R.China Department of Respiratory Medicine Ningbo Hangzhou Bay HospitalNingboZhejiangP.R.China Department of Orthopedic Surgery Second Affiliated Hospital(Jiande Branch)Zhejiang University School of MedicineHangzhouZhejiangP.R.China Department of Breast Surgery Second Affiliated HospitalZhejiang University School of MedicineZhejiang UniversityHangzhouZhejiangP.R.China

Bone is a common organ affected by metastasis in various advanced cancers,including lung,breast,prostate,colorectal,and *** a patient is diagnosed with bone metastasis,the patient’s quality of life and overall survival are significantly reduced owing to a wide range ofmorbidities and the increasing difficulty of *** studies have shown that bone metastasis is closely related to bone microenvironment,especially bone immune ***,the effects of various immune cells in the bone microenvironment on bone metastasis remain ***,we described the changes in various immune cells during bone metastasis and discussed their related ***,adipocytes,and other non-immune cells closely related to bone metastasis were also *** review also summarized the existing treatment methods and potential therapeutic targets,and provided insights for future studies of cancer bone metastasis.

关键词： bone metastasis bone microenvironment cancer immune cell therapy

异速轧制和常规轧制对7075铝板力学性能的影响

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有色金属科学与工程 2024年第3期15卷 400-406页

作者：武磊何兵覃文东覃铭运城职业技术大学智能制造与数智矿山学院山西运城044000 广西壮族自治区铝基新材料工程研究中心广西百色533000 百色学院材料科学与工程学院广西百色533000 吉利百矿研究院有限公司广西百色533000

为进一步开发更高效的7075铝板轧制工艺,本文围绕异速轧制和常规轧制对7075铝板力学性能的影响开展了研究,并采用了4个不同的轧制累积变形率。实验分2步进行,第1步为轧板弯曲程度随异速比变化实验,将轧板弯曲内面朝向水平底面放置,测量... 详细信息

为进一步开发更高效的7075铝板轧制工艺,本文围绕异速轧制和常规轧制对7075铝板力学性能的影响开展了研究,并采用了4个不同的轧制累积变形率。实验分2步进行,第1步为轧板弯曲程度随异速比变化实验,将轧板弯曲内面朝向水平底面放置,测量弯曲内面最高点距离水平底面距离y,以及弯曲内面与水平底面的两接触点距离x,用y/x值表征轧板的弯曲程度;第2步为异速和常规轧制实验。实验结果表明:当异速比为1.12时,轧板弯曲程度最大,y/x值最高达到0.22218;虽然采用了相同的设计累积变形率,但异速轧制和常规轧制的轧板实际累积变形率相差1%~2%;两种方式轧制的轧板在实际累积变形率达到76%时,T6热处理过程中再结晶进行得更加充分,再结晶生成的中小尺寸晶粒增多;经过6道次异速轧制和常规轧制的7075铝板,T6热处理后,断口中可见大量尺寸小于1μm的韧窝,并且在韧窝底部出现弥散析出的尺寸小于100 nm的微小第二相粒子,异速轧制7075铝板中韧窝尺寸较常规轧制7075铝板中更细小;与常规轧制相比,采用异速轧制的7075铝板的强度有一定提高,但提高幅度不大;常规轧制的7075铝板在达到一定变形量时会出现强度峰值,而异速轧制的7075铝板的强度随轧制累积变形率升高而持续提高。

关键词：异速轧制 7075铝板变形率异速比力学性能

中国体育旅游精品项目的空间分布特征及其影响因素

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经济地理 2024年第1期44卷 185-196页

作者：赵书虹李琴云南大学工商管理与旅游管理学院云南昆明650000

体育旅游精品项目是体育运动的重要空间载体和体育旅游产业发展的重要依托。基于ArcGIS空间分析方法从总体和区域层面对中国1079个体育旅游精品项目的空间分布特征进行分析,结合地理探测器、叠置分析方法剖析影响精品项目空间分布的主... 详细信息

体育旅游精品项目是体育运动的重要空间载体和体育旅游产业发展的重要依托。基于ArcGIS空间分析方法从总体和区域层面对中国1079个体育旅游精品项目的空间分布特征进行分析,结合地理探测器、叠置分析方法剖析影响精品项目空间分布的主要因子。研究发现:(1)从总体层面来看,体育旅游精品项目呈“东多西少,南多北少”的空间结构特征;空间分布类型为集聚型;空间密度呈“单极多核”分布形态。(2)从区域层面来看,省域尺度精品项目分布极不均衡,空间分布密度差异较大,上海、北京、天津的分布密度最高;分布具有明显空间自相关特征,热点区集中于长三角地区和西南地区。市域尺度精品项目的区域不平衡程度大于省域尺度,热点区主要分布于长三角、京津冀、黔中城市群及青海东部、甘肃西北部等地区。(3)体育旅游精品项目的空间分布受地理环境、社会经济发展、资源禀赋、体育旅游政策环境、交通建设与住宿接待水平等5个维度因素的共同作用和影响,各影响因素对精品项目空间分异的解释度差异明显。

关键词：体育旅游精品项目空间分布体育产业社会经济发展资源禀赋体育旅游政策

On the solution of Stokes equations for plane boundaries

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Acta Mechanica Sinica 2017年第1期33卷 62-64页

作者： y. qin R. Sun Department of Engineering Mechanics Shanghai Jiao Tong University Shanghai 200240 China

In a recent paper (Li et al., Acta Mech. Sin. 31, 32-44, 2015), the authors claimed that the general solution of steady Stokes flows can be compactly expressed using only two harmonic functions. They present two cases of a flat plate translating through a viscous fluid. The present paper shows that such a two-harmonic solution does not describe the rotation of a circular plate in an unbounded fluid and thus confirms that at least three independent harmonics are required to express the general solution of Stokes equations.

关键词： Stokes flow General solution Harmonics

基于离心机实验的海上风力机大直径单桩桩土作用研究

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太阳能学报 2024年第8期45卷 537-545页

作者：柳涛秦梦飞施伟中国电建集团华东勘测设计研究院有限公司杭州311122 大连理工大学建设工程学部大连116024

地基基础是海上风电结构所受载荷的最终承载者,针对中国沿海软弱超固结软土地基开展力学特性研究。通过离心模型试验方法对单桩基础形式的静力特性进行分析和对比。研究结果揭示通过离心模型试验模拟桩基础的有效性,该方法能准确捕捉现... 详细信息

地基基础是海上风电结构所受载荷的最终承载者,针对中国沿海软弱超固结软土地基开展力学特性研究。通过离心模型试验方法对单桩基础形式的静力特性进行分析和对比。研究结果揭示通过离心模型试验模拟桩基础的有效性,该方法能准确捕捉现场地基的应力分布及桩与土体之间的相互作用特性。在软黏土环境中,针对不同直径的单桩进行的分析表明,其变形行为及桩土相互作用机制呈现出显著的差异性。规范中的p-y曲线不适用于中国近海土质,据试验结果提出地基无量纲水平抗力系数随埋深变化和与循环应力幅值相关的循环弱化因子计算模型。

关键词：海上风电风力机桩土作用离心模型试验循环加载 p-y模型土抗力系数

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