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埋入式电阻的热特性研究

电子元件与材料 2005年第12期24卷 61-63页

作者：陈清远容锦泉香港理工大学工业及系统工程学系

通过对电阻的热功率、表面积的分析,研究了PCB板中的埋入式电阻的热特性,给出了PCB板中埋入式电阻的温升的解析表达式。计算了埋入式电阻在PCB板中的不同位置的温升情况,详细讨论了多个埋入式电阻的温升与单个电阻温升的关系,得到了计... 详细信息

通过对电阻的热功率、表面积的分析,研究了PCB板中的埋入式电阻的热特性,给出了PCB板中埋入式电阻的温升的解析表达式。计算了埋入式电阻在PCB板中的不同位置的温升情况,详细讨论了多个埋入式电阻的温升与单个电阻温升的关系,得到了计算具有多个埋入式电阻的PCB板的温升的方法。在设计PCB板时,大功率埋入式电阻应安排在板的中部,埋入式电阻的相互距离要分散并远离PCB板表面。

关键词：电子技术埋入式电阻 PCB板热特性设计规则

Multi-drug Resistant Uropathogenic Escherichia coli and Its Treatment by Chinese Medicine

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Chinese Journal of Integrative Medicine 2017年第10期23卷 763-769页

作者： LIU Shi-wei XU Xiao-yang XU Jie yUAN Jiu-yun WU Wei-kang ZHANG Ning chen Ze-liang Department of Endocrinology Wangjing Hospital China Academy of Chinese Medical Sciences Institute of Disease Control and Prevention Academy of Military Medical Sciences Department of Clinical Laboratory The First Affiliated Hospital of Soochow University Institute of Integrative Medicine Sun Yat-sen University

Objectives： To investigate the resistance and virulence profiles of uropathogenic Escherichia coli（UPEC） and its treatment by Chinese medicine（CM） Fuzheng Qingre Lishi Formula（扶正清热利湿方, FQLF）. Methods： UPEC strains were isolated from recurrent urinary tract infections（UTIs） patients. Patient sensitivities to 17 antibiotics were tested by the disk diffusion method. Virulence genes were screened by plolymerase chain reaction. A mouse model was constructed using a multi-drug resistant and virulent UPEC strain and treated with FQLF or the antibiotic imipenem. The treatment efficacy was evaluated by bacterial clearance from urine and the urinary organs. Results： A total of 90 UPEC strains were collected, and 94.4% of the isolates were resistant to at least 1 antibiotic. Approximately 66.7% of the UPEC strains were multi-drug resistant. More than one virulence gene was found in 85.6% of the isolates. The extended-spectrum β-lactamases（ESBL）-positive strains were more resistant than the negative ones. The virulence gene number was positively correlated with the resistance number（P〈0.05）. A mouse model was successful y constructed using UPEC10. Treatment with either FQLF or antibiotics significantly cleared bacteria from the mouse urine after 14 days. In the untreated control, the bacteria lasted for 28 days. FQLF treatment of the UTI mouse model greatly reduced the bacterial number in the kidney and bladder, but could not completely clear the bacteria. Conclusions： Multi-drug resistance is common among UPEC isolates, and the resistance is positively related with virulence. FQLF could treat UPEC UTIs, but could not completely clear the bacteria from the host.

关键词： urinary tract infection uropathogenic Escherichia coli Chinese medicine multi-drug resistance

土壤盐分对Bt棉铃壳中Bt蛋白含量和氮代谢生理的影响

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应用生态学报 2018年第8期29卷 2583-2589页

作者：王永慧陈建平孙艳茹张祥陈德华江苏沿海地区农业科学研究所/农业部沿海盐碱地农业科学观测实验站江苏盐城224401 扬州大学江苏省作物遗传生理重点实验室江苏扬州225009

为探讨土壤盐分对转Bt基因抗虫棉棉铃抗虫性的影响,以2个Bt棉品种‘新棉33B’(盐敏感)和‘中07’(耐盐)为试验材料,设置5个土壤盐分水平(0、0.15%、0.30%、0.45%和0.60%),采用盆栽法研究了不同土壤盐分下Bt棉盛铃期铃壳Bt蛋白含量变化... 详细信息

为探讨土壤盐分对转Bt基因抗虫棉棉铃抗虫性的影响,以2个Bt棉品种‘新棉33B’(盐敏感)和‘中07’(耐盐)为试验材料,设置5个土壤盐分水平(0、0.15%、0.30%、0.45%和0.60%),采用盆栽法研究了不同土壤盐分下Bt棉盛铃期铃壳Bt蛋白含量变化及氮代谢生理特征.结果表明:铃壳中Bt蛋白含量随土壤盐分的升高而降低,与对照相比(0%),盐敏感型品种在土壤盐分0.15%、耐盐型品种在0.30%以上时,铃壳中Bt蛋白含量显著下降.相同土壤盐分水平下,花后30 d(DPA)铃壳Bt蛋白含量下降幅度明显大于10 DPA.在铃壳Bt杀虫蛋白含量下降显著时,其可溶性蛋白含量明显下降,硝酸还原酶(NR)和谷氨酸丙酮酸转氨酶(GPT)活性显著下降;游离氨基酸含量、蛋白酶和肽酶活性在土壤盐分0.30%以上时显著增加.以上结果说明,土壤盐分影响铃壳的氮代谢,从而引起Bt蛋白合成减弱,中度以上土壤盐分水平会引起Bt蛋白分解加强,从而导致杀虫蛋白表达水平进一步下降.

关键词： Bt蛋白土壤盐分 Bt棉氮代谢生理

Exogenous GSH protects tomatoes against salt stress by modulating photosystem Ⅱ efficiency, absorbed light allocation and H2O2-scavenging system in chloroplasts

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Journal of Integrative Agriculture 2018年第10期17卷 2257-2272页

作者： ZHOU yan DIAO Ming CUI Jin-xia chen Xian-jun WEN Ze-lin ZHANG Jian-wei LIU Hui-ying Department of Horticulture Agricultural College Shihezi University Shihezi 832003 P.R. China Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction Crops Shihezi 832003 P.R.China

The effects of exogenous GSH（reduced glutathione）on photosynthetic characteristics,photosystem Ⅱ efficiency,absorbed light energy allocation and the H2O2-scavenging system in chloroplasts of salt-stressed tomato（Solanum lycopersicum L.）seedlings were studied using hydroponic experiments in a *** of exogenous GSH ameliorated saline-induced growth inhibition,the disturbed balance of Na＋ and Cl- ions and Na＋/K＋ ratios,and the reduction of the net photosynthetic rate（Pn）.GSH also increased the maximal photochemical efficiency of PSⅡ（Fv/Fm）,the electron transport rate（ETR）,the photochemical quenching coefficient（qP）,and the non-photochemical quenching coefficient（NPQ）.In addition,GSH application increased the photochemical quantum yield（y（Ⅱ））and relative deviation from full balance between the photosystems（β/α-1）and decreased the PSⅡ excitation pressure（1-qP）and quantum yield of non-regulated energy dissipation（y（NO））in leaves of salt-stressed tomatoes without BSO（L-buthionine-sulfoximine,an inhibitor of key GSH synthesis enzymeγ-glutamylcysteine synthetase）or with ***,the addition of GSH depressed the accumulation of H2O2 and malondialdehyde（MDA）,induced the redistribution of absorbed light energy in PSⅡ reaction centers,and improved the endogenous GSH content,GSH/GSSH ratio and activities of H2O2-scavenging enzymes（including superoxidase dismutase（SOD）,catalase（CAT）,peroxidase（POD）and key enzymes in the AsA-GSH cycle and Grx system）in the chloroplasts of salt-stressed plants with or without ***,GSH application alleviates inhibition of salt-induced growth and photosynthesis mainly by overcoming stomatal limitations,improving the PSⅡ efficiency,and balancing the uneven distribution of light energy to reduce the risk of ROS generation and to mediate chloroplast redox homeostasis and the antioxidant defense system to protect the chloroplasts from oxidative ***,GSH may be used as a potential tool for alleviating salt stress in to

关键词： tomato reduced glutathione NaCl stress chloroplast photosynthesis antioxidant enzyme

AMPK activation by peri-sciatic nerve administration of ozone attenuates CCl-induced neuropathic pain in rats

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Journal of Molecular Cell Biology 2017年第2期9卷 132-143页

作者： Lijuan Lu Cailong Pan Lu chen Liang Hu Chaoyu Wang yuan Han yanjing yang Zhixiang cheng Wen-Tao Liu Department of Pain Affiliated Drum Tower Hospital of Medical School of Nanjing University Nanjing 210008 China Jiangsu Key Laboratory of Neurodegeneration Department of Pharmacology Nanjing Medical University Nanjing 210029 China State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing 210009 China Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University Nanjing 210029 China Department of Pain Management & Cancer Biotherapy Center The Second Affiliated Hospital of Nanjing Medicat University Nanjing 210029 China

Neuropathic pain is a debilitating clinical condition with few efficacious treatments, warranting development of novel therapeu- tics. Ozone is widely used as an alternative therapy for many different pain conditions, with exact mechanisms still elusive. In this study, we found that a single peri-sciatic nerve injection of ozone decreased mechanical aUodynia and thermal hyperalgesia, and normalized the phosphorylation of protein kinase C y, N-methyI-D-aspartate receptor, and extracellular signal-regulated kinase in a chronic constriction injury （CCI） model in rat sciatic nerve. Meanwhile, ozone significantly suppressed CCI-induced activation of spinal microgUa. More importantly, the anti-nociceptive effect of ozone depended on the activation of 5＇-adenosine monophosphate （AMP）-activated protein kinase （AMPK）, which was proved by the fact that the phosphorylated AMPK level increased during the ozone therapy and AMPK antagonist abolished the effect of ozone in vivo and in vitro. In addition, direct injection of AMPK agonist could replicate the anti-nociceptive effect of ozone in CCI rats. In conclusion, our observations indicate that peri-sciatic nerve injection of ozone activates AMPK to attenuate CCI-induced neuropathic pain.

关键词： AMPK ozone therapy neuropathic pain

Synergistic Effect of Schwann Cells and Retinoic Acid on Differentiation and Synaptogenesis of Hippocampal Neural Stem Cells in vitro

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Biomedical and Environmental Sciences 2006年第3期19卷 219-224页

作者： XUE-BAO ZHANG yUAN-SHAN ZENG WEI ZHANG yA-yUN chen yI XIONG SUI-JUN chen Division of Neuroscience Department of Histology and Embryology Zhongshan Medical College Sun Yat-Sen University Guangzhou 510080 Guangdong China

Objective To investigate the synergistic effect of Schwann cells （SCs） and retinoic acid （R.A） on differentiation and synaptogenesis of neural stem cells （NSCs） derived from hippocampus of neonatal rats. Methods The classical method for 2×2 factorial analysis experiment was used to assess synergistic action of SCs and RA. NSCs were treated with R.A, SCs, and SCs ＋ RA in DMEM/F12 with 0.5% fetal bovine serum for six days, respectively. Double immunofluorescent staining was used to detect the differentiation of NSCs including nestin, glial fibrillary acidic protein （GFAP） and Map2. The expression of PSD95 was used to demonstrate synaptogenesis. Results After NSCs were treated with RA or SCs, the expression of nestin and GFAP was significantly decreased while the expression of Map2 and PSD95 was significantly increased in comparison with the control. Factorial ANOVA showed that interactions between SCs and RA could induce the expression of Map2 and PSD95. Conclusion SCs and RA could promote synergistically the neuronal differentiation and synaptogenesis of hippocampal neural stem cells in vitro while they decreased the astrocytes and nestin positive NSCs.

关键词： Neural stem cell Neuronal differentiation PSD95 Schwann cells Retinoic acid

Study of a 560-mm-long stripline kicker for HIT 10 MeV electron accelerator

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Radiation Detection Technology and Methods 2021年第3期5卷 373-378页

作者： Jianbin Zhu Hua Shi Bowen Bai Shu Zhang Zhandong Zhang Bin chen yunlong Chi Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China

Purpose A stripline kicker is required for Harbin Institute of Technology(HIT)10 MeV electron accelerator to scan electrons horizontally in two directions,and the maximum deflecting angle is 65 *** this study,a 560-mm-long stripline kicker was designed to achieve good field uniformity and odd mode impedance *** In order to reduce power reflections and meet field uniformity requirement,we optimized the cross section of the kicker main *** and optimization of the stripline kicker were finished by *** The field uniformity is 2%in the 60 mm(x)×80 mm(y)region,as *** impedance matching and manufacturing,the transmission odd mode impedance was finally chosen to 45Ω,which indicated a reflecting loss of less than−50 dB below 1 *** maximum field is less than 7 MV/m with a maximum pulse voltage of±22 kV between the two electrodes when the electrons pass through the *** The physical design of the stripline kicker has been finished,and the simulation results satisfied the requirements.

关键词： Linac Stripline kicker Field uniformity

Effects of chilling tolerance induced by spermidine pretreatment on antioxidative activity,endogenous hormones and ultrastructure of indica-japonica hybrid rice seedlings

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Journal of Integrative Agriculture 2016年第2期15卷 295-308页

作者： ZENG yan-hua ZAHNG yu-ping XIANG Jing WU Hui chen Hui-zhe ZHANG yi-kai ZHU De-feng College of Agronomy Nanjing Agricultural University State Key Laboratory of Rice Biology China National Rice Research Institute

Spermidine（Spd） is known to be involved in the regulation of plant responses to chilling stress and counteract the adverse effect of stress *** activities,endogenous hormones and ultrastructure change under chilling stress were investigated in indica-japonica hybrid rice seedlings.12-d-old seedlings were subjected to exogenous Spd（1 mmol L^（-1）） and then a chilling stress（6℃,4 d） was induced,followed by a subsequent recovery（25℃,4 d）.Results showed that malondialdehyde（MDA） and proline content were enhanced significantly,whereas shoot fresh and dry weights decreased during chilling stress and after recovery;chlorophyll content of chilling-stressed seedlings increased slightly but declined after recovery;additionally,total soluble sugar,sucrose,fructose and starch contents increased significantly during chilling stress,and only soluble sugar and fructose contents were observed in increase after recovery;chilling stress-induced increases in superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT） activities,but declined after recovery,and the level of ascorbate peroxidase was lower during chilling stress and after recovery;however,endogenous indole-3-acetic acid（IAA）,zeatin riboside（ZR）,gibberellic acid（GA_3）,and abscisic acid（ABA） levels were induced decreased compared with Spd *** microscopic analysis revealed that chilling stress-induced destruction of the chloroplast envelope during chilling stress and increased the number of plastoglobuli along with aberrations in thylakoid membranes after *** contrast,exogenous Spd protected rice seedlings from chilling-induced injuries in terms of lower malondialdehyde,proline and carbohydrates accumulation coupled with increased endogenous hormones *** recovery,Spd pretreatment chilling-exposed seedlings showed higher activities of antioxidant enzymes and normal physiological function of *** results suggest that Spd could promote effectively chilling tolera

关键词： polyamines chilling stress antioxidative activity endogenous hormones ultrastructure indica-japonica hybrid rice(Oryza sativa L.)

Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus(BmNPV)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第2期13卷 111-117页

作者： Xing-wei XIANG Rui yANG Lin chen Xiao-long HU Shao-fang yU Cui-ping CAO Xiao-feng WU College of Animal Sciences Zhejiang UniversityHangzhou 310058China

In the late phase of Bombyx mori nucleopolyhedrovirus(BmNPV)infection,a large amount of polyhedra appear in the infected cell nucleolus,these polyhedra being dense protein crystals protecting the incorporated virions from the harsh *** investigate whether the foreign protein could be immobilized into the polyhedra of BmNPV,two recombinant baculoviruses were generated by a novel BmNPV polyhedrin-plus(polh +)Bac-to-Bac system,designated as vBmBac(polh +)-enhanced green fluorescent protein(EGFP)and vBmBac(polh +)-LacZ,which can express the polyhedrin and foreign protein *** microscopy analysis showed that all viruses produced polyhedra of normal *** fluorescence can be apparently detected on the surface of the vBmBac(polh +)-EGFP polyhedra,but not the BmNPV *** analysis and anti-desiccation testing confirmed that EGFP was embedded in the *** expected,the vBmBac(polh +)-LacZ polyhedra contained an amount of LacZ and had a higherβ-galactosidase *** dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting were also performed to verify if the foreign proteins were immobilized into *** study provides a new inspiration for efficient preservation of useful proteins and development of new pesticides with toxic proteins.

关键词： Bombyx mori nucleopolyhedrovirus Polyhedra Foreign protein Protein immobilization