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Insecticidal activities and chemical components of alcohol extract from leaves of Rhodendron dauricum l.

Journal of Forestry Research 2011年第1期22卷 133-135页

作者： SUN mo-long WANG Tian-miao College of Science Northeast Forestry University Harbin 150040 P. R.China School of Chemistry and molecular Engineering East China Universityof Science and Technology Shanghai 200237 P. R. China

The extract from leaves of Rhododendron dauricum l. was extracted with 95% alcohol by common method for studying its insecticidal activities. The chemical components of the alcohol extract and relative contents were analyzed by GC-MS. The insecticidal activities of the alcohol extract were tested on the 2nd？3rd instar larvae of lymantria dispar l. for five days. Five concentrations of the extract samples were designed as 50, 10, 5, 1, and 0.5 g？l？1. The results show that the alcohol extracts from leaves of R. dauricum exhibited insecticidal activities against larvae of l. dispar. The corrected mortality of larvae of l. dispar for was over 50% for both contact toxicity and stomach toxicity at the extract concentration of ≥ 5 g·l？1 after five days of application. The insecticidal activity in contact toxicity is more effect than stomach toxicity for the alcohol extract. Twenty compounds, with total GC relative contents of 93.81% in the alcohol extract from leaves of R. dauricum were identified. The main chemical components in the extract are：（1） 4,5-Dihydro-5-oxo-3-（p-tolyl） isoxazole, with a relative content of 40.03%; （2） 1,3-Benzenediol, 5-methyl-2-（3,7,11-trimethyl-2,6,10- dodecatrienyl）-, （E,E）-, the relative content 18.27%; （3） 3,6-Diphenyl- 1,2,3,4,5,6,7,8-octahydro-1,8-acridinedione, the relative content 3.89%; （4） 6H-[1,2,4]Triazolo[1,5-a]indole, 4a,5,7,8,8a,9-hexahydro-9- methylene-, the relative content 2.99%; （5） 7-Amino-4-methyl-1,8- naphthyridin-2-ol, the relative content 2.64%; （6） 4-Methyl-2,6- dihydroxyquinoline, the relative content 2.63%; （7） 2,4,6- Triaminoquinazoline, the relative content 2.27%; （8） 2（1H）-Quinolinone, 4-hydroxy-1-methyl-, the relative content 2.02%.

关键词： Rhodendron dauricum l. insecticidal activities component

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Feasibility of establishing model of Parkinson disease by injecting 6-hydroxydopamine at different parts of the nigrostriatal pathway in the brain of rats

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Neural Regeneration Research 2006年第2期1卷 126-129页

作者： yuefei Shen Xuean mo Guifang long Department of Neurology First Affiliated Hospital GuangxiMedical University Nanning530021 Guangxi Zhuang Autonomous Region China

BACKGROUND： Previous researches found that animal models with Parkinson disease （PD） could be established by injecting 6-hydroxydopamine （6-OHDA） into medial forebrain bundle （MFB）, substantia nigra compacta （SNC） and caudate-putamen complex （CPU） of the nigrostriatal pathway. OBJECTIVE ： To compare behavioral, biochemica 6-OHDA injections in the areas of MFB, SNC and DESIGN： Controlled observational study and histological properties of these rats undergoing the CPU respectively. SEI-IING： Department of Neurology, First Affiliated Hospital of Guangxi Medical University MATERIAlS： A total of 64 adult female SD rats weighing 180-230 g were provided by the Animal Experimental Center of Guangxi Medical University. 6-OHDA （Sigma Company, USA）; Brain solid positioner （Standard model 51600, Stoelting Co., Il, USA）; rotational monitoring of little animal （type Ql-1, USA）; high liquid chromatography （HlC, Waters Company）. METHOOS： The experiment was carried out in the Medical Experimental Center of Guangxi Medical University from February to December 2005. ① According to digital table, 64 SD rats were divided into MFB group, SNC group, CPU group and control group with 16 in each group. On the basis of the brain atlas of Paxinos, rats in the first three groups were injected with 5 μl 6-OHDA into right MFB （0 mm of line of incisor tooth, A/P 4.4 mm, l/R 1.2 mm, ON -7.8 mm）, SNC （line of incisor tooth just equal to horizon, A/P -4.8 mm, l/R 1.6 mm, ON -7.8 mm） and CPU （0 mm of line of incisor tooth, A/P 1.2 mm, l/R 2.7 mm, ON -5.4 mm）, respectively. The rats in control group were injected with 5 μl ascorbic acid solution （2 g/l）. One week after operation, 0.1 g/l apomorphine （Apo, 0.05 mg/kg） was subcutaneously injected into neck and then rotational behavior induced by Apo was recorded once a week for 8 weeks. The PD models were considered successful only when rotational times more than or equal to 7 times per minute. Eight weeks after operation, micro-perfusion was used to

关键词： Feasibility of establishing model of Parkinson disease by injecting 6-hydroxydopamine at different parts of the nigrostriatal pathway in the brain of rats

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Application of Nanometer-Size Titanium Dioxide in Extreme-Trace V(Ⅴ) Analysis

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Wuhan University Journal of Natural Sciences 2006年第2期11卷 437-440页

作者： QIAN Shahua lUO yan mo Shaobo WENG yue College of Resources and Environmental Sciences Wuhan University Wuhan 430079 Hubei China College of Chemistry and molecular Sciences Wuhan University Wuhan 430072 Hubei China

The adsorption properties of nanometer-size TiO2 for V（Ⅴ ） were studied. The adsorption rate could reach above 99~ when the pH values were at the range of 4 10. The adsorption balance time was 10 min , the saturation capacity of adsorption of nanometer-size TiO2 to V（Ⅴ） was 6.43 mg per gram. Using 2 ml 1.5 mol · l^-1 NaOH as elution, we found the elution rate could reach 95%. A novel method of extreme-trace V（Ⅴ ） preconeentration with nanometer-size titanium dioxide and determination by graphite furnace atomic absorption spectroscopy （GFAAS） was advanced. The detection limit（3σ）of the method was 0.61μg ·l^-1 , and the relative standard deviation was 8.1% （n=6） of 2.5μg ·l-^1 V（Ⅴ）. Environmental samples experiments were also conducted to test the feasibility of the method, and it came out that the recovery rates were between 91.2% and 102.0%.

关键词： nanometer-size TiO2 V(Ⅴ) graphite furnace atomic absorption spectroscopy ( GFAAS ) extreme-trace analysis

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Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells

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World Journal of Gastroenterology 2010年第5期16卷 618-624页

作者： liang, Xue-Song Zhou, ying li, Chen-Zhong Wan, mo-Bin Second Mil Med Univ Changhai Hosp Dept Infect Dis Shanghai 200433 Peoples R China Second Mil Med Univ Dept Biochem & Mol Biol Shanghai 200433 Peoples R China

AIM:To investigate if and how programmed death type-1(PD-1)expression affects the natural course of hepatitis B virus(HBV)infection. METHODS:Sixty-four patients in different natural stages of chronic HBV infection were enrolled in this ***-1 expression in total T cells was detected by flow *** of total CD8+T cell responses and proliferation in relation to PD-1 expression levels were analyzed with intracellular staining and PD-1/ PD-l1 blockage. RESUlTS:The PD-1 expression in T cells was dynamically changed during the natural course of chronic HBV infection,did not significantly increase in the immune tolerance phase,and returned to normal in the inactive virus carrier *** of the PD-1/PD-l1 pathway could not affect the T-cell response in the immune tolerance and inactive virus carrier stages of chronic HBV ***,it could significantly restore the T-cell response in the immune clearance stage of chronic HBV ***,the PD-1 expression level in T cells was associated with the alanine aminotransferase level during the immune clearance stage of chronic HBV infection. CONClUSION:The PD-l/PD-l1 pathway plays a different role in T-cell response during the natural course of chronic HBV infection.

关键词： Programmed death type-1 Hepatitis B virus Chronic hepatitis B Natural stage CD8+T cell Serum viral load Programmed death ligand T cell response

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Evaluation of hyperreflective foci as a prognostic factor of visual outcome in retinal vein occlusion

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International Journal of Ophthalmology(English edition) 2017年第4期10卷 605-612页

作者： Bin mo Hai-ying Zhou Xuan Jiao Feng Zhang Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology & Visual Sciences Key Laboratory

AIM：To evaluate the potential role of hyperreflective foci（HF） as a prognostic indicator of visual outcome in patients with macular edema（ME） due to retinal vein occlusion（RVO）.METHODS：We retrospectively reviewed 50 eyes of 50 patients with ME due to ischemic central retinal vein occlusion（CRVO）,non-ischemic CRVO and branch retinal vein occlusion（BRVO） who were treated with anti-vascular endothelial growth factor（anti-VEGF） at Beijing Tongren Eye Center from January 2013 to July *** patients underwent best-corrected visual acuity（BCVA）,spectral domain optical coherence tomography（SD-OCT） at baseline and *** factors were evaluated and compared among three groups as baseline and final BCVA,central retinal thickness（CRT）,external limiting membrane（ElM） status and the numbers of HF in different *** linear regression analysis was employed to analyze the relationship between baseline HF and final *** of HF before and after treatment were evaluated ***：Among three groups,HF could be located in each retinal layers,as well as in vitreous *** mean HF in outer retinal layer（ORl） at baseline was 5.29±8.48 in ischemic CRVO with intact ElM,1.93±2.76 in non-ischemic CRVO,and 1.75±2.05 in *** disrupted ElM,the mean HF in ORl *** was statistically difference of HF in ORl between intact and disrupted *** numbers of HF in ORl were associated with poor visual outcome among three ***,HF in inner retinal layer（IRl） and vitreous cavity were not associated with poor visual ***,the baseline HF in ORl and vitreous cavity reduced significantly in nonischemic CRVO and BRVO after anti-VEGF ***：The numbers of HF in ORl are prognostic factors associated with the final BCVA in patients with ME due to RVO after anti-VEGF treatment.

关键词： hyperreflective foci retinal vein occlusion treatment optical coherence tomography

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Fabrication and characterization of curcumin-loaded silk fibroin/P（llA-Cl） nanofibrous scaffold

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Frontiers of Materials Science 2014年第4期8卷 354-362页

作者： yuan lIAN Jian-Chao ZHAN Kui-Hua ZHANG Xiu-Mei mo College of Materials and Textile Engineering Jiaxing University Jiaxing 314001 China Biomaterials and Tissue Engineering laboratory College of Chemistry and Chemical Engineering and Biological Engineering Donghua University Shanghai 201620 China

Curcumin exhibited excellent properties including antioxidant, anti- inflammatory, antiviral, antibacterial, antifungal, anticancer, and anticoagulant activities. In this study, curcumin was incorporated into silk fibroin （SF）/poly（l-lactic acid-co-e- caprolactone）（P（llA-Cl）） nanofibrous scaffolds via electrospinning, and changes brought about by raising the curcumin content were observed： SEM images showed that the average nanofibrous diameter decreased at the beginning and then increased, and the nanofibers became uniform; FTIR showed that the conformation of SF transforming from random coil form to β-sheet structure had not been induced, while SF conformation converted to β-sheet after being treated with 75% ethanol vapor; XRD results confirmed that the crystal structure of （P（llA-Cl）） had been destroyed; The mechanical test illustrated that nanofibrous scaffolds still maintained good mechanical properties. Further, curcumin-loaded nanofibrous scaffolds were evaluated for drug release, antioxidant and antimicrobial activities in vitro. The results showed that curcumin presented a sustained release behavior from nanofibrous scaffolds and maintained its free radical scavenging ability, and such scaffolds could effectively inhibit S. aureus growth （〉 95%）. Thus, curcumin-loaded SF/P（llA-Cl） nanofibrous scaffolds might be potential candidates for wound dressing and tissue engineering scaffolds.

关键词： curcumin SF/P(llA-Cl) nanofibrous scaffold control release

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Impact of Fenton and ozone on oxidation of wastewater containing nitroaromatic compounds

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Journal of Environmental Sciences 2008年第6期20卷 675-682页

作者： Fares Al momani mo’ayyad Shawaqfah Ahmad Shawaqfeh mohammad Al-Shannag Chemical Engineering Department Mutah University Civil and Environmental Engineering Department Mu tah University Faculty of Engineering Technology Al-Balqa Applied University

Fenton and ozone treatment was investigated at laboratory scale for the degradation of aqueous solutions of nitrobenzene （NB）. Effects of reactants concentration （O3, H2O2, and Fe（Ⅱ））, temperature, and pH on NB degradation were monitored. Reaction kinetic of these processes was also assessed. A rapid reaction took place for Fenton process at higher initial concentration of H2O2, higher temperatures, and more acidic conditions （pH 3）. Similarly, ozonation reaction exhibited rapid rates for higher ozone dose, higher temperatures, and more basic conditions （pH 11）. Complete NB degradation in 65 min was achieved using Fenton process. The conditions of complete elimination of 100 mg/l of initial NB concentration, were 250 mg/l of H202 concentration, pH 3, and 10 mg/l of Fe（Ⅱ） concentration. Under these conditions, 55% organic carbon elimination was achieved. Total organic carbon mineralization was attained in 240 rain reaction time by Fenton process with 900 mg/l of H202 concentration, and 30 mg/l of Fe（Ⅱ） concentration. Fenton reaction showed a pseudo-first order kinetic; the reaction rate constant was ranged from 0.0226 to 0.0658 min^-1. Complete NB degradation was also achieved for an ozone dose of the order of 2.5 g/l. The ozonation was studied at different ozone doses, different initial pH （7-11） and at different temperatures （15-35℃）. NB ozonation kinetic was represented by a bi-molecular kinetic model which was reduced to pseudo-first order kinetic. The pseudo-first order reaction rate constant was determined to increase at 20℃ from 0.004 to 0.020 min^-1 as the used ozone increased from 0.4 to 1.9 g/l.

关键词： nitro-aromatics chemical oxidation organic matter content

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Thirty-eight spider species (Arachnida: Araneae) from China, Indonesia, Japan and Vietnam

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Zoological Systematics 2024年第1期49卷 4-98页

作者： yejie lin Shuqiang li Haolin mo Xihao Wang Institute of Zoology Chinese Academy of SciencesBeijing 100101China Key laboratory of Wildlife Evolution and Conservation in mountain Ecosystem of Guangxi Nanning Normal UniversityNanning 530001China Jinan licheng No.2 High School Jinan 250109China

Four new genera in three spider families are reported, namely langlibaitiao lin &li, gen. nov., of Dictynidae O. Pickard-Cambridge, 1871, with lathys inaffecta li, 2017(♂♀,China: Guangxi) as the type species;Huoyanluo lin & li, gen. nov., of Macrobunidae Petrunkevitch, 1928, with Huoyanluo ruanxiaoqi lin & li, sp. nov.(♂, China: yunnan) as the type species and Gushangzao lin & li, gen. nov. and Gyro lin & li, gen. nov., of Theridiidae Sundevall, 1833, with Gushangzao shiqian lin & li, sp. nov.(♂♀, China: Xizang) and Gyro zeppeli lin & li, sp. nov.(♀, China: yunnan) as the type species, respectively. Further 31 new species in 19 spider families are reported, including Agelenidae C.l. Koch, 1837:Troglocoelotes doul lin & li, sp. nov.(♀, China: Guangxi) and T. ruanxiaowu lin & li, ***.(♀, China: Guangxi);Dictynidae: langlibaitiao zhangshun lin & li, sp. nov.(♂, China:Hainan);Hahniidae Bertkau, 1878: Hahnia zhuyifani lin & li, sp. nov.(♀, China: yunnan);liocranidae Simon, 1897: Platnick xintongi lin & li, sp. nov.(♂♀, China: Xizang);Macrobunidae: Funny yanqing lin & li, sp. nov.(♂♀, China: Xizang) and Huoyanluo zhangzezhongi lin & li, sp. nov.(♂, China: yunnan);Mysmenidae Petrunkevitch, 1928:Phricotelus yangxiong lin & li, sp. nov.(♂♀, China: Chongqing);Nemesiidae Simon, 1889:Raveniola shixiu lin, Wang & li, sp. nov.(♀, China: Guangxi) and R. xiezhen lin, Wang & li,sp. nov.(♂♀, China: yunnan);Nesticidae Simon,1894: Speleoticus sicet lin & li, sp. nov.(♂♀,China: Sichuan);Ochyroceratidae Fage, 1912: Speocera yiduoi lin & li, sp. nov.(♂, China:Guangdong);Oecobiidae Blackwall, 1862: Uroctea chenyui lin & li, sp. nov.(♂♀, China:Guangxi);Oonopidae Simon, 1890: Orchestina xiebao lin & li, sp. nov.(♀, China:Chongqing);Philodromidae Thorell, 1869: Apollophanes lujiani lin & li, sp. nov.(♂♀, China:Guangxi) and Psellonus dawanqu lin & li, sp. nov.(♂♀, China: Guangdong);Salticidae Blackwall, 1841: Coccorchestes spark lin & li, sp. nov.(♂♀, Indonesia, West Papua),Onomastus zhuwu

关键词： Diagnosis new combination new genus new species Asia.

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Electrospinning nanofiber scaffolds for soft and hard tissue regeneration

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Journal of Materials Science & Technology 2020年第24期59卷 243-261页

作者： Xianrui Xie yujie Chen Xiaoyu Wang Xiaoqing Xu yihong Shen Atta ur Rehman Khan Ali Aldalbahi Allison E.Fetz Gary l.Bowlin mohamed El-Newehy Xiumei mo State Key laboratory for modification of Chemical Fibers and Polymer Materials College of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghai201620China Department of Chemistry College of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia Department of Biomedical Engineering University of MemphisMemphisTN38152USA

Tissue engineering is an interdisciplinary field that integrates medical,biological,and engineering expertise to restore or regenerate the functionality of healthy tissues and *** three fundamental pillars of tissue engineering are scaffolds,cells,and *** nanofibers have been successfully used as scaffolds for a variety of tissue engineering applications because they are biomimetic of the natural,fibrous extracellular matrix(ECM)and contain a three-dimensional(3D)network of interconnected *** this review,we provide an overview of the electrospinning process,its principles,and the application of the resultant electrospun nanofibers for tissue *** first briefly introduce the electrospinning process and then cover its principles and standard equipment for biomaterial ***,we highlight the most important and recent advances related to the applications of electrospun nanofibers in tissue engineering,including skin,blood vessels,nerves,bone,cartilage,and tendon/ligament ***,we conclude with current advancements in the fabrication of electrospun nanofiber scaffolds and their biomedical applications in emerging areas.

关键词： Nanofibers Electrospinning Tissue engineering Regeneration Biomedical application

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Antibiotics along an alpine river and in the receiving lake with a catchment dominated by grazing husbandry

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Journal of Environmental Sciences 2022年第5期34卷 374-382页

作者： Jianwei Dong Dandan yan Kangle mo Qiuwen Chen Jianyun Zhang yuchen Chen Zhiyuan Wang State Key laboratory of Hydrology-Water Resources and Hydraulic Engineering Nanjing Hydraulic Research InstituteNanjing 210029China Center for Eco-Environmental Research Nanjing Hydraulic Research InstituteNanjing 210029China yangtze Institute for Conservation and Green Development Nanjing 210029China School of Marine Science and Engineering Nanjing Normal UniversityNanjing 210023China

The livestock breeding industries face overuse of antibiotics,which has been intensively studied in recent ***,the occurrence and fate of antibiotics as well as their potential threats to the aquatic environments in alpine and arid regions remain *** study investigated the relationship of the occurrence and concentrations of antibi-otics between the Kaidu River and Bosten lake in a typical alpine basin in *** spots with antibiotic pollution source were *** antibiotic concentrations in river water and suspended sediment(SPS)were 2.20-99.4 ng/l and 1.03-176 ng/*** dominant antibi-otics were tetracyclines,sulphacetamide,and ofloxacin in river water and sulfonamides,clarithromycin,roxithromycin,and ofloxacin in *** apparent differences in pollution sources and landscapes in different reaches led to the obvious spatial patterns of antibiotics in the Kaidu *** partition coefficient of antibiotic between SPS and water phases for sulfonamides than tetracyclines was because that tetracyclines strongly responded to clay contents while sulfonamides significantly responded to organic carbon contents in *** were significant differences in detected antibiotic categories between the river and the ***(especially ciprofloxacin and enrofloxacin)were detected in the lake while sulphacetamide was only detected in the ***,the surrounding husbandry and aquaculture around the Bosten lake was an important antibiotic pollution source in addition to inputs from the Kaidu *** research suggested that alpine lakes could be an important sink of antibiotics in alpine dry regions,and thus impose greater threats to the aquatic ecosystem.

关键词： Antibiotics lotic-lentic contamination Suspended sediment Partition

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