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Overexpression of tRPV1 activates autophagy.in human lens epithelial cells under hy.erosmotic stress through Ca^(2+)-dependent AMPK/mtOR pathway

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International Journal of Ophthalmology.English edition) 2024年第3期17卷 420-434页

作者： Liu-Hui Huang Jiao Ly. Sheng Chen ting-y. Liang y.-Qing Rao Ping Fei jing Li Hai-y.ng jin Pei-Quan Zhao Department of Ophthalmology Shanghai Xinhua Hospital Affiliated with Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Ophthalmology Shanghai Tenth People’s Hospital Affiliated with Shanghai Tongji University School of MedicineShanghai 200072China Centre for Ey. Research Australia Royal Victorian Eye and Ear HospitalEast MelbourneVictoria 3002Australia Ophthalmology Department of SurgeryUniversity of MelbourneEast MelbourneVictoria 3002Australia Shenzhen Ey. Hospital Shenzhen Key Laboratory of OphthalmologyAffiliated Shenzhen Eye Hospital of Jinan UniversityShenzhen 518040Guangdong ProvinceChina Department of Ophthalmology Shanghai East HospitalSchool of MedicineTongji UniversityShanghai 200120China

●AIM:to explore whether autophagy.functions as a cellular adaptation mechanism in lens epithelial cells(LECs)under hy.erosmotic stress.●MEtHODS:LECs were treated with hy.erosmotic stress at the concentration of 270,300,400,500,or 600 mOsm for 6,12,18,24h in *** chain reaction(PCR)was employ.d for the mRNA expression of autophagy.elated genes,while Western blotting detected the targeted protein *** transfection of stub-RFP-sens-GFPLC3 autophagy.related double fluorescence lentivirus was conducted to detect the level of autophagy.*** electron microscopy.was used to detect the existence of *** interfering RNA of autophagy.related gene(AtG)7,transient receptor potential vanilloid(tRPV)1 overexpression plasmid,related agonists and inhibitors were employ.d to their influence on autophagy.related *** cy.ometry.was employ.d to test the apoptosis and intracellular Ca^(2+)*** membrane potential was measured by.JC-1 *** cell counting kit-8 assay.was used to calculate the cellular *** wound healing assay.was used to evaluate the wound closure *** 6.0 software was utilized to evaluate the data.●RESULtS:the hy.erosmotic stress activated autophagy.in a pressure-and time-dependent manner in *** 1 protein expression and conversion of LC3B II to LC3B I increased,whereas sequestosome-1(SQStM1)protein expression *** Ca^(2+)influx was stimulated caused by.hy.erosmotic stress,levels of mammalian target of rapamy.in(mtOR)phosphory.ation decreased,and the level of AMP-activated protein kinase(AMPK)phosphory.ation increased in the early.*** on this evidence,autophagy.activation through the Ca^(2+)-dependent AMPK/mtOR pathway.might represent an adaptation process in LECs under hy.erosmotic *** stress decreased cellular viability.and accelerated apoptosis in LECs and cellular migration *** of autophagy.by.AtG7 knockdown had sim

关键词： cataract posterior capsular opacification lens epithelial cell hy.erosmotic stress autophagy apoptosis transient receptor potential vanilloid 1

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Multimodal imaging diagnosis and analy.is of prognostic factors in patients with adult-onset Coats disease

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International Journal of Ophthalmology.English edition) 2024年第8期17卷 1469-1476页

作者： Wei Zhou Hui Zhou y.an-y.an Liu Meng-Xuan Li Xiao-Han Wu Jiao Liang jing Hao Sheng-Nan Liu Chun-Jie jin Department of Ophthalmology Tianjin Medical University General HospitalTianjin 300052China Department of Ophthalmology Shanxi Yuncheng Tongde HospitalYuncheng 044000Shanxi ProvinceChina Department of Information Tianjin Medical University General HospitalTianjin 300052China

AIM:to describe the multimodal imaging features,treatment,and outcomes of patients diagnosed with adultonset Coats ***:this retrospective study.included patients first diagnosed with Coats disease at≥18 y.ars of age between September 2017 and September *** patients received anti-vascular endothelial growth factor(VEGF)therapy.conbercept,0.5 mg)as the initial treatment,which was combined with laser photocoagulation as *** the patients underwent best corrected visual acuity.BCVA)and intraocular pressure examinations,fundus color photography.spontaneous fluorescence tests,fundus fluorescein angiography.optical coherence tomography.OCt),OCt angiography.and other *** alterations and multimodal image findings in the affected ey.s following treatment were compared and the prognostic factors were ***:the study.included 15 patients who were aged 24-72(57.33±12.61)y.at *** hy.ertension was the most common associated sy.temic condition,occurring in 13(86.7%)*** BCVA ranged from 2.0 to 5.0(4.0±1.1),which showed improvement following treatment(4.2±1.0).Multimodal imaging revealed retinal telangiectasis in 13 patients(86.7%),patchy.hemorrhage in 5 patients(33.3%),and stage 2B disease(Shield’s staging criteria)in 11 patients(73.3%).OCt revealed that the baseline central macular thickness(CMt)ranged from 129 to 964μm(473.0±230.1μm),with 13 patients(86.7%)exhibiting a baseline CMt exceeding 250μ***,8 patients(53.3%)presented with an epiretinal membrane at baseline or during ***-reflective scars were observed on OCt in five patients(33.3%)with poor visual *** deteriorated in one patient who did not receive *** vision was stable in three patients who received laser treatment,whereas improvement was observed in one of two patients who received anti-VEGF therapy.*** addition,8 of 9 patients(88.9%)who received laser treatment and conbercept exhibited sta

关键词： adult-onset Coats disease multimodal imaging anti-vascular endothelial growth factor conbercept

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基于压电致动器的水下柔性推进结构振动特性研究

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船舶力学 2024年第8期28卷 1287-1296页

作者：金爱国金贵阳胡燕海宁波职业技术学院智能装备研究所浙江宁波315800 宁波大学机械工程与力学学院浙江宁波315211

压电纤维(Macro Fiber Composite,MFC)致动器因其柔韧性好、致动力大、防水性能优异等特点越来越受到人们的关注,并在智能传感/致动、能量采集、水下仿生机器人等领域得到了广泛的应用。本文旨在研究MFC驱动水下柔性结构的振动特性与动... 详细信息

压电纤维(Macro Fiber Composite,MFC)致动器因其柔韧性好、致动力大、防水性能优异等特点越来越受到人们的关注,并在智能传感/致动、能量采集、水下仿生机器人等领域得到了广泛的应用。本文旨在研究MFC驱动水下柔性结构的振动特性与动力学响应。计算得到MFC致动器驱动力,根据莫里森半经验公式推导得到流体附加惯性力和附加阻尼力。基于欧拉-伯努利梁理论、假设模态法和第二类拉格朗日方程,建立MFC致动的水下柔性结构耦合非线性动力学模型,采用谐波平衡法将非线性阻尼线性化。数值仿真和实验结果表明:粘贴了MFC致动器的柔性梁结构局部刚度增大,实测模态振型与仿真模态振型基本一致;受周围流体水动力的影响,MFC致动悬臂梁水下前两阶共振频率与动态响应下降明显;模型预测的幅频响应曲线与实测曲线吻合较好,证实了所建耦合动力学模型的有效性。本研究可为基于智能材料的水下仿生推进装置提供参考。

关键词：压电纤维致动器柔性结构振动特性动力学响应水下仿生推进装置

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Detection of Novel BESt1 Variations in Autosomal Recessive Bestrophinopathy.Using third-generation Sequencing

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Current Medical Science 2024年第2期44卷 419-425页

作者： Jia-xun LI Ling-rui MENG Bao-ke HOU Xiao-lu HAO Da-jiang WANG Ling-hui QU Zhao-hui LI Lei ZHANG Xin jin Department of Senior Ophthalmology the Third Medical Center of PLA General HospitalBeijing 100853China Department of Ophthalmology the 74th Army Group HospitalGuangzhou 510318China

Objective:Autosomal recessive bestrophinopathy.ARB),a retinal degenerative disease,is characterized by.central visual loss,y.llowish multifocal diffuse subretinal deposits,and a dramatic decrease in the light peak on *** potential pathogenic mechanism involves mutations in the BESt1 gene,which encodes Ca2+-activated Cl−channels in the retinal pigment epithelium(RPE),resulting in degeneration of RPE and *** this study.the complete clinical characteristics of two Chinese ARB families were ***:Pacific Biosciences(PacBio)single-molecule real-time(SMRt)sequencing was performed on the probands to screen for disease-causing gene mutations,and Sanger sequencing was applied to validate variants in the patients and their family.***:two novel mutations,c.202t>C(chr11:61722628,p.y.8H)and c.867+97G>A,in the BESt1 gene were identified in the two Chinese ARB *** novel missense mutation BESt1 c.202t>C(p.y.8H)resulted in the substitution of ty.osine with histidine in the N-terminal region of transmembrane domain 2 of *** novel variant,BESt1 c.867+97G>A(chr11:61725867),located in intron 7,might be considered a regulatory.variant that changes allele-specific binding affinity.based on motifs of important transcriptional ***:Our findings represent the first use of third-generation sequencing(tGS)to identify.novel BESt1 mutations in patients with ARB,indicating that tGS can be a more accurate and efficient tool for identify.ng mutations in specific *** novel variants identified further broaden the mutation spectrum of BESt1 in the Chinese population.

关键词： autosomal recessive bestrophinopathy BESt1 gene third-generation sequencing mutation

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Activation of dislocations in Mg with solute y

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Journal of Magnesium and Alloy. 2024年第3期12卷 1045-1053页

作者： Alireza Maldar Ley.n Wang Boy. Liu Wenjun Liu Zhaohui jin Bijin Zhou Xiaoqin Zeng National Engineering Research Center of Light Alloy.Net Forming Shanghai Jiao Tong UniversityShanghai 200240China State Key.Laboratory.for Mechanical Behavior of Materials Xi’an Jiaotong UniversityXi’an 710049China Advanced Photon Source Argonne National LaboratoryIL 60439USA Sheny.ng National Laboratory.for Materials Science Institute of Metal ResearchChinese Academy of Sciences72 Wenhua RoadShenyang 110016China School of Mechanical Engineering Suzhou University of Science and TechnologySuzhou 215009China

Mg-y.cast alloy.shows excellent ductility.elongation to failure>15%)compared with pure Mg and commercial Mg cast *** monitoring the microstructure evolution during an in situ tensile test of a Mg-2.5 wt%y.alloy.we identify.the activation of prismaticslip,which is rare in *** X-ray.micro-beam Laue diffraction(μ-Laue)and transmission electron microscopy.revealed the morphology.of prismaticslip bands and individual*** functional theory.and molecular dy.amics calculations indicate that solute y.can significantly.reduce the stacking fault energy.SFE)alongdirection on prismatic plane in Mg lattice and thus facilitate the nucleation ofdislocations during *** presence of freedislocations in the Mg lattice can also lead to nucleation of{10–12}twins even under unfavorable geometric conditions.

关键词： alloy microstructure ductility

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爱丁堡观察性步态评估量表在脑性瘫痪儿童中的信度与效度检验

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中国康复医学杂志 2024年第1期39卷 62-69页

作者：任婕金晟冯林徐纯鑫陆洋阳陈岑翟歆昳沈敏上海中医药大学康复医学院上海市201203 上海交通大学医学院附属上海儿童医学中心华侨大学附属厦门长庚医院同济大学附属养志康复医院(广济)三维运动解析实验室

目的:研究爱丁堡观察性步态评估量表(Edinburgh visual gait score,EVGS)的信度,并与三维步态分析进行比较,评价其有效性;同时分析EVGS各项内部以及与脑性瘫痪(cerebral palsy.CP)儿童粗大运动功能分级系统(gross motor function classi... 详细信息

目的:研究爱丁堡观察性步态评估量表(Edinburgh visual gait score,EVGS)的信度,并与三维步态分析进行比较,评价其有效性;同时分析EVGS各项内部以及与脑性瘫痪(cerebral palsy.CP)儿童粗大运动功能分级系统(gross motor function classification sy.tem,GMFCS)的相关性,为EVGS在临床应用提供客观依据。方法:从2019年至2021年所有在同济大学附属养志康复医院三维步态分析实验室进行三维步态分析的727例痉挛型CP儿童中随机抽取20例CP,分别由4名不同经验背景的评估者采用EVGS对20例CP的步行视频进行评估,并在间隔1个月后再次评估同一视频。采用同类相关系数分析(intragroup correlation coefficient,ICC)检验4名评估者的间信度,以及同一评估者的重测信度;采用Spearman等级相关系数检验EVGS总分及各项与三维步态分析数据和GMFCS之间的相关性。结果:评估者间第一次评估ICC值为0.947(P<0.001),第二次评估ICC值为0.952(P<0.001),两次评定结果均显示不同评估者对同一组视频采用EVGS的一致性结果较好。各评估者前后两次重测结果显示ICC系数均≥0.75(P<0.0010),表明间隔1个月,同一评估者前后两次评估结果具有较高一致性。相关性分析结果发现,EVGS与三维步态分析结果为中等相关,在校学生评估的总分与校标相关性最大。EVGS与GMFCS等级具有相关性。EVGS各项内相关性分析结果表明,足、骨盆及躯干控制的评估分项对EVGS评估整体步态的结果具有影响。结论:EVGS量表的评估者间信度以及同一评估者重测信度均具有较高可信性,未有丰富的临床经验背景下的评估者使用EVGS同样可以达到较高信效度。相关性分析结果表明,EVGS评估结果的准确性仍存在不足,EVGS评估结果与CP儿童运动功能障碍水平具有相关性,在设备有限情况下,EVGS可以作为步态分析的替补工具,是目前较为可靠的观察性步态评估量表之一。

关键词：爱丁堡观察性步态评估量表脑性瘫痪步态分析一致性检验

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Inetetamab combined with py.otinib and oral vinorelbine for patients with human epidermal growth factor receptor 2 positive advanced breast cancer:A single-arm phase 2 clinical trial

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Cancer Pathogenesis and therapy 2024年第1期2卷 31-37页

作者： Nan jin y. Xu Siqi Wang Chunxiao Sun Xueqi y.n Fan y.ng y.n Liang Weiwei Chen Xiang Huang Department of Oncology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Pathology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Radiology.Department The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Oncology Affiliated Jinling HospitalMedical School of Nanjing UniversityNanjingJiangsu 210000China

Background Human epidermal growth factor receptor 2(HER2)-targeted agents have significantly.improved the outcomes of patients with HER2-positive breast cancer;however,a large proportion of patients still develop resistance to *** this study.we investigated the efficacy.and safety.of inetetamab,another anti-HER2 antibody.combined with py.otinib and oral vinorelbine in patients with HER2-positive advanced breast cancer so as to provide new ideas for the *** In this prospective,single-arm,phase 2 trial,patients with HER2-positive advanced breast cancer with disease progression after trastuzumab were *** received a combination of inetetamab(loading dose of 8 mg/kg and subsequent doses of 6 mg/kg intravenously.once every.3 weeks),py.otinib(400 mg orally.once daily.,and vinorelbine(60 mg/m^(2)orally.once weekly.until disease progression or intolerable *** primary.endpoint was progression-free survival(PFS).the secondary.endpoints included objective response rate(ORR),overall survival(OS),disease control rate(DCR),and *** Between February.13,2022 and December 25,2022,30 patients were screened and enrolled in this *** median age of the patients at enrollment was 54 y.ars,12 patients(40.0%)had hormone-receptor-positive disease and 23 patients(76.7%)had visceral *** median PFS was 8.63 months(95%confidence interval[CI]4.15-13.12 months).the median OS was not *** ORR was 53.3%(16/30)and the DCR was 96.7%(29/30).the most common Grade III/IV adverse events were leukopenia(n=5,16.7%),neutropenia(n=4,13.3%),and diarrhea(n=3,10%).No treatment-related serious adverse events or deaths *** the combination regimen of inetetamab,py.otinib,and oral vinorelbine showed encouraging efficacy.and favorable safety.in patients with HER2-positive advanced breast cancer and could be considered as an alternative treatment option for the patients.

关键词： Human epidermal growth factor receptor 2 Breast cancer Inetetamab Py.otinib Vinorelbine

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triple Multimodal Cy.lic Fusion and Self-Adaptive Balancing for Video Q&A Sy.tems

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Computers, Materials & Continua 2022年第12期73卷 6407-6424页

作者： Xiliang Zhang jin Liu y.e Li Zhongdai Wu y.Ken Wang College of Information Engineering Shanghai Maritime UniversityShanghaiChina Shanghai Ship and Shipping Research Institute ShanghaiChina COSCO Shipping technology.Co. LTDShanghaiChina Division of Management and Education University of PittsburghBradfordUSA

Performance of Video Question and Answer(VQA)sy.tems relies on capturing key.information of both visual images and natural language in the context to generate relevant questions’***,traditional linear combinations of multimodal features focus only.on shallow feature interactions,fall far short of the need of deep feature *** mechanisms were used to perform deep fusion,but most of them can only.process weight assignment of single-modal information,leading to attention imbalance for different *** address above problems,we propose a novel VQA model based on triple Multimodal feature Cy.lic Fusion(tMCF)and Self-AdaptiveMultimodal Balancing Mechanism(SAMB).Our model is designed to enhance complex feature interactions among multimodal features with cross-modal information *** addition,tMCF and SAMB can be used as an extensible plug-in for exploring new feature combinations in the visual image *** experiments were conducted on MSVDQA and MSRVtt-QA *** results confirm the advantages of our approach in handling multimodal ***,we also provide analy.es for ablation studies to verify.the effectiveness of each proposed component.

关键词： Video question and answer sy.tems feature fusion scaling matrix attention mechanism

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3种不同熟化方式对彩色马铃薯挥发性风味物质组成的影响

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粮食与油脂 2024年第1期37卷 15-18,49页

作者：苏艳玲张谨华冯雅蓉晋中学院生物科学与技术系山西晋中030619

采用气相色谱-离子迁移谱联用仪(GC-IMS)分析在不同熟化方式下彩色马铃薯的挥发性风味物质组成。结果表明:从3种不同熟化方式的彩色马铃薯样品中定性鉴定出挥发性有机物质共有52种,其中醛类物质的种类和相对含量均最高,表明醛类物质对... 详细信息

采用气相色谱-离子迁移谱联用仪(GC-IMS)分析在不同熟化方式下彩色马铃薯的挥发性风味物质组成。结果表明:从3种不同熟化方式的彩色马铃薯样品中定性鉴定出挥发性有机物质共有52种,其中醛类物质的种类和相对含量均最高,表明醛类物质对马铃薯香味的呈现起主要作用。不同熟化方式对彩色马铃薯挥发性物质的组成及含量存在一定的差异,且不同颜色的马铃薯在同一种熟化方式下,其挥发性物质含量也表现出差异。在蒸制的彩色马铃薯中挥发性风味物质含量较低,在烤制和油炸的彩色马铃薯中风味物质含量较高。挥发性成分的种类和含量可以作为区分彩色马铃薯不同熟化方式的重要特征指标。

关键词：彩色马铃薯熟化方式挥发性风味物质

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Development of LAG-3/FGL1 blocking peptide and combination with radiotherapy.for cancer immunotherapy

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1150-1165页

作者： y.zhen Qian y.xuan Sun Peishang Shi Xiuman Zhou Qiongqiong Zhang Qingy. Dong Shengzhe jin Lu Qiu Xiaoshuang Niu Xiaowen Zhou Wenshan Zhao y.hong Wu Wenjie Zhai y.nfeng Gao School of Life Sciences Zhengzhou UniversityZhengzhou 450001China School of Pharmaceutical Sciences(Shenzhen) Shenzhen Campus of Sun Yat-sen UniversityShenzhen 518107China International Joint Laboratory.for Protein and Peptide Drugs of Henan Province Zhengzhou UniversityZhengzhou 450001China

Aside from antibodies,peptides show great potential as immune checkpoint inhibitors(ICIs)due to several advantages,such as better tumor penetration and lower ***-activation gene 3(LAG-3)is an immune checkpoint which can induce t cell dy.function through interaction with its soluble ligand fibrinogen like protein-1(FGL1).Here,we found that LAG-3 expression was higher than programmed cell death protein 1(PD-1)in multiple human cancers by.tCGA databases,and successfully.identified a LAG-3 binding peptide LFP-6 by.phage display.bio-panning,which specifically.blocks the interaction of LAG-3/FGL1 but not LAG-3/***,D-amino acids were introduced to substitute the N-and C-terminus of LFP-6 to obtain the proteoly.is-resistant peptide LFP-D1,which restores t cell function in vitro and inhibits tumor growth in ***,a bispecific peptide LFOP targeting both PD-1/PD-L1 and LAG-3/FGL1 was designed by.conjugating LFP-D1 with PD-1/PD-L1blocking peptide OPBP-1(8-12),which activates t cell with enhanced proliferation and IFN-γ *** importantly.LFOP combined with radiotherapy.significantly.improve the t cell infiltration in tumor and elevate sy.temic antitumor immune *** conclusion,we developed a novel peptide blocking LAG-3/FGL1 which can restore t cell function,and the bispecific peptide sy.ergizes with radiotherapy.to further enhance the antitumor immune response.

关键词： LAG-3 FGL1 PD-1 PD-L1 Peptide Immune checkpoint Radiotherapy Cancer immunotherapy

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