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Chinese Science Bulletin 2013年第28期58卷 3535-3540页

作者： BAI WenKun y.ng shaoling SHEN E ZHAng JiZhen SHEN Zhiy.ng HU Bing Shanghai Jiao Tong University Affiliated 6th People's Hospital Shanghai Institute of Ultrasound in Medicine

The objective of the present study was to investigate whether ultrasound combined with microbubbles induces apoptotic cell death in androgen-independent prostate cancer cells and to identify the probable *** used ultrasound in continuous wave mode with a frequency of 21 kHz and a spatial-average temporal-average intensity of 46 mW/*** combined with microbubbles(200μL SonoVue)was used to treat androgen-independent human prostate cancer PC-3 cells for 30 ***-3 cells were divided into three groups:the control group,the ultrasound group and the ultrasound combined with microbubbles *** after treatment,trypan blue exclusion was used to assess cell *** apoptosis at 24 h after treatment was measured using transmission electron microscopy and flow *** blotting was used to evaluate the expression of the apoptosis-related proteins,Bcl-2 and *** combined with microbubbles had a minimal effect on the viability of PC-3cells and induced minimal levels of cell *** level of apoptosis in PC-3 cells induced by this modality was significantly higher than in controls(12.77±0.31%vs.2.56±0.22%,P<0.01).Treatment with ultrasound combined with microbubbles increased the expression of Bax,a pro-apoptotic protein,and decreased the expression of Bcl-2,an anti-apoptotic *** was concluded that ultrasound combined with microbubbles induces apoptotic cell death in human prostate cancer PC-3 cells through down-regulation of Bcl-2 and up-regulation of Bax.

关键词：超声波频率 Bcl-2 细胞死亡结合治疗 Bax 微泡诱导 Western印迹法

PbrRALF2-elicited reactive oxygen species signaling is mediated by the PbrCrRLK1L13-PbrMPK18 module in pear pollen tubes

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Horticulture Research 2021年第1期8卷 3042-3054页

作者： Xiaobing Kou Jiangmei Sun Peng Wang Danqi Wang Peng Cao Jing Lin youhong Chang shaoling Zhang Juyou Wu Center of Pear Engineering Technology Research State Key Laboratory of Crop Genetics and Germplasm EnhancementCollege of HorticultureNanjing Agricultural University210095 NanjingChina Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement 210014 NanjingChina

Rapid alkalinization factors(RALFs)are cysteine-rich peptides that play important roles in a variety of biological processes,such as cell elongation and immune *** studies in Arabidopsis have shown that RALFs regulate pollen tube growth via plasma membrane receptor-like kinases(RLKs).However,the downstream signal transduction mechanisms of RLKs in pollen tubes are ***,we identified PbrRALF2,a pear(Pyrus bretschneideri)pollen RALF peptide that inhibits pollen tube *** found that PbrRALF2 interacts with a malectin-like domain-containing RLK,*** relative affinity between PbrRALF2 and PbrCrRLK1L13 was at the submicromolar level,which is consistent with the values of ligand–receptor kinase pairs and the physiological concentration for PbrRALF2-mediated inhibition of pollen tube *** binding to its extracellular domain,PbrRALF2 activated the phosphorylation of PbrCrRLK1L13 in a dose-dependent *** further showed that the MAP kinase PbrMPK18 is a downstream target of PbrCrRLK1L13 that mediates PbrRALF2-elicited reactive oxygen species(ROS)*** excessive accumulation of ROS inhibits pollen tube *** show that MPK acts as a mediator for CrRLK1L to stimulate ROS production,which might represent a general mechanism by which RALF and CrRLK1L function in signaling pathways.

关键词： RALF reactive alkali

The osteoclastic activity in apical distal region of molar mesial roots affects orthodontic tooth movement and root resorption in rats

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International Journal of Oral Science 2024年第2期16卷 322-332页

作者： Wenhao Zheng Xiaofeng Lu Guangjin Chen yufeng Shen Xiaofei Huang Jinfeng Peng Jiajia Wang y.ng yin Wencheng Song Mengru Xie shaoling yu Lili Chen Department of Stomatology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina School of Stomatology Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration WuhanChina Department of Stomatology The First Affiliated HospitalSchool of MedicineShihezi UniversityShiheziChina

The utilization of optimal orthodontic force is crucial to prevent undesirable side effects and ensure efficient tooth movement during orthodontic ***,the sensitivity of existing detection techniques is not sufficient,and the criteria for evaluating optimal force have not been yet ***,by employ.ng 3D finite element analysis methodology,we found that the apical distal region(A-D region)of mesial roots is particularly sensitive to orthodontic force in ***-resistant acidic phosphatase(TRAP)-positive osteoclasts began accumulating in the A-D region under the force of 40 grams(g),leading to alveolar bone resorption and tooth *** the force reached 80 g,TRAP-positive osteoclasts started appearing on the root surface in the A-D ***,micro-computed tomography revealed a significant root resorption at 80 ***,the A-D region was identified as a major contributor to whole root *** was determined that 40 g is the minimum effective force for tooth movement with minimal side effects according to the analysis of tooth movement,inclination,and *** findings suggest that the A-D region with its changes on the root surface is an important consideration and sensitive indicator when evaluating orthodontic forces for a rat ***,our investigations into this region would aid in offering valuable implications for preventing and minimizing root resorption during patients’orthodontic treatment.

关键词： movement roots offering

The involvement of PybZIPa in light-induced anthocyanin accumulation via the activation of PyUFGT through binding to tandem G-boxes in its promoter

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Horticulture Research 2019年第1期6卷 82-94页

作者： Hainan Liu Jun Su y.ngfan Zhu Gaifang yao Andrew C.Allan Charles Ampomah-Dwamena Qun Shu Kui Lin-Wang shaoling Zhang Jun Wu Centre of Pear Engineering Technology Research State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural University210095 NanjingChina Institute of Horticulture Yunnan Academy of Agricultural Sciences650205 KunmingChina The New Zealand Institute for Plant&Food Research Limited AucklandNew Zealand School of Biological Sciences University of AucklandAucklandNew Zealand

To gain insight into how anthocyanin biosynthesis is controlled by light in fruit,transcriptome and metabolome analyses were performed in the Chinese sand pear cultivar“Mantianhong”(Pyrus pyrifolia)after bagging and bag *** investigated transcriptional and metabolic changes and gene-metabolite correlation *** tests of anthocyanin content and transcriptional changes revealed that 1,530 transcripts were strongly correlated with 15 anthocyanin derivatives(R2>0.9,P-valueng associated with flavonoid metabolism,transcriptional regulation,and light *** connection network revealed a new photosensitive transcription factor,PybZIPa,that might play an important role during light-induced anthocyanin *** overexpression of PybZIPa promoted anthocyanin accumulation in pear and strawberry fruit as well as tobacco *** luciferase and y1H assays further verified that PybZIPa directly activated the expression of PyUFGT by binding to tandem G-box motifs in the promoter,which was key to differential anthocyanin accumulation in debagged pear skin,and the number of G-box motifs affected the transcriptional activation of PyUFGT by *** results indicate that the light-induced anthocyanin biosynthesis regulatory mechanism in pear differs from that described in previous reports suggesting that a bZIP family member co-regulates anthocyanin biosynthesis with other transcription factors in apple and *** was found that,in response to light,PybZIPa promoted anthocyanin biosynthesis by regulating important transcription factors(PyMyB114,PyMyB10,and PyBBX22)as well as structural genes(PyUFGT)via binding to G-boxes within *** activation was amplified by the self-binding of PybZIPa to activate its own ***,we demonstrate the utility of a multiomics integrative approach for discovering new functional genes and pathways underly.ng light-induced anthocyanin

关键词： cultivar activation metabolism

PbMC1a/1b regulates lignification during stone cell development in pear(Pyrus bretschneideri)fruit

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Horticulture Research 2020年第1期7卷 1915-1927页

作者： Xin Gong Zhihua Xie Kaijie Qi Liangyi Zhao yazhou yuan Jiahui Xu Weikang Rui Katsuhiro Shiratake Jianping Bao Shahrokh Khanizadeh shaoling Zhang Shutian Tao College of Horticulture Nanjing Agricultural UniversityNanjingChina Laboratory of Horticultural Science Nagoya UniversityNagoyaJapan College of Plant Science Tarim UniversityAla’er CityChina ELM Consulting Inc. St-LazareCanada Eastern Cereal and Oilseed Research Centre Agriculture and Agri-Food CanadaOttawaCanada State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjingChina

Programmed cell death(PCD)and secondary cell wall(SCW)thickening in pear fruit are accompanied by the deposition of cellulose and lignin to form stone *** is an important protease for development,tissue renewal and *** understanding of the molecular mechanism whereby pear(Pyrus)metacaspase promotes PCD and cell wall lignification is still *** this study,the Metacaspases gene family(PbMCs)from *** was ***1a/1b was associated with lignin deposition and stone cell formation by physiological data,semiquantitative real-time polymerase chain reaction(RT-PCR)and quantitative RT-PCR(qRT-PCR).Relative to wildtype(WT)Arabidopsis,the overexpression of PbMC1a/1b increased lignin deposition and delayed growth,thickened the cell walls of vessels,xylary fibers and interfascicular fibers,and increased the expression of lignin biosynthetic *** two-hybrid(y2H),bimolecular fluorescence complementation(BiFC)and GST pull-down assays indicated that the PbMC1a/1b protein physically interacted with ***,the transient expression of PbMC1a/1b and PbRD21 led to significant changes in the expression of genes and lignin contents in pear fruits and flesh *** results indicate that PbMC1a/1b plays an important role in cell wall lignification,possibly by interacting with PbRD21 to increase the mRNA levels of some lignin synthesis-associated genes and promote the formation of stone cells in pear fruit.

关键词： fibers walls transient

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轻金属与钢异种金属电弧熔钎焊的冶金机理

轻金属与钢异种金属电弧熔钎焊的冶金机理

作者：邵玲兰州理工大学

学位级别：硕士

本文针对铝-钢异种金属焊接过程中极易生成脆性的金属间化合物而降低接头力学性能的问题,提出一种新型高效低热输入的脉冲旁路耦合电弧GMAW(Pulsed Double-electrode gas metal arc welding, Pulsed DE-GMAW)方法,能够精确控制焊接热输... 详细信息

本文针对铝-钢异种金属焊接过程中极易生成脆性的金属间化合物而降低接头力学性能的问题,提出一种新型高效低热输入的脉冲旁路耦合电弧GMAW(Pulsed Double-electrode gas metal arc welding, Pulsed DE-GMAW)方法,能够精确控制焊接热输入从而控制铝-钢异种金属界面区金属间化合物的生长,来进行铝与镀锌钢焊接试验。此外也用这种方法进行了镁与镀锌钢异种金属的焊接试验。用ER5356铝合金焊丝在镀锌钢板上,在焊丝总电流保持不变,不同的Pulsed DE-GMAW焊接参数下进行平板堆焊试验。结果显示随着旁路电流的增大,焊缝宽度显著变小,且焊缝堆高明显变高。通过对连接界面区的微观组织进行观察和分析,表明连接界面区的金属间化合物由Fe2Al5和FeAl3金属间化合物组成,且母材热输入大时,FeA13析出得多,随着母材热输入的减小,FeAl3析出减少到FeAl3没有析出。对FeAl3金属间化合物的吉布斯自由能进行计算和比较,表明在焊接过程中Fe2Al5金属间化合物先生成,再在冷却过程中FeAl3金属间化合物生成或析出。其次对铝与镀锌钢接头剥落面进行XRD分析,确定有Fe2Al5Zn0.4金属间化合物形成。这表明Zn不仅在铝与镀锌钢界面上起到润湿的作用,它还在铝与镀锌钢熔钎焊过程中参与反应形成化合物。比较含有Fe2Al5Zno.4金属间化合物层与FeA13、 Fe2Al5金属间化合物的维氏硬度,初步确定Fe2Al5Zn0.4为非脆性金属间化合物,并进一步在测试结果的基础上对Fe2A15Zn0,4金属间化合物的形成机理进行探讨,提出Fe2Al5Zn0.4金属间化合物是Zn通过扩散进入A1-Fe金属间化合物中而结合形成的。接着分别用Al-Si焊丝和Al-Mg焊丝进行铝合金和镀锌钢异种金属的搭接试验,获得成形良好,变形小的搭接接头。通过比较Al-Si焊丝和Al-Mg焊丝接头的微观组织,得出两种焊丝与镀锌钢界面的金属间化合物层都由Fe2Al5和FeAl3组成,但它们的形态存在差异。Al-Si焊缝与镀锌钢界面的金属间化合物层在焊缝侧呈针状,在镀锌钢侧呈平齐状;然而Al-Mg焊缝与镀锌钢界面的金属间化合物层在焊缝侧呈锯齿状,在镀锌钢侧呈舌状。在Al-Si焊丝接头的剥落面上检测出Feo.7Al3Sio.3相。结果表明Si通过减少Fe通过Al-Fe-Si金属间化合物的扩散率来抑制Fe2Al5金属间化合物的形成。最后用AZ92A镁合金焊丝在镀锌钢板上进行平板堆焊试验,通过调整焊接工艺参数,能在最佳的焊接参数下得到熔宽均匀,成形良好的焊缝。通过对焊接接头界面区微观组织的观察与分析,及对焊缝剥落面进行物相的确定发现有Mg17Al12、Mgo.97Zno.o3和Fe4Zn9金属间化合物形成。结果表明镁与镀锌钢板异种金属电弧熔钎焊时Mg和Fe都与Zn反应形成了金属间化合物。

关键词：脉冲旁路耦合电弧GMAW熔钎焊铝-镀锌钢焊接接头界面金属间化合物

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基于吸光度加和性的仪器分析实验设计

实验技术与管理 2018年第8期35卷 199-201页

作者：丁明珍彭璟马少玲南京大学金陵学院化学与生命科学学院江苏南京210089

建立了一种同时测定日落黄和柠檬黄2种合成食用色素含量的紫外—可见光谱分析方法。利用吸光度加和性原理,无需分离,可直接对市售饮料中日落黄和柠檬黄含量进行同时测定,操作简单、实验成本低、结果准确。将该方法应用到仪器分析开放性... 详细信息

建立了一种同时测定日落黄和柠檬黄2种合成食用色素含量的紫外—可见光谱分析方法。利用吸光度加和性原理,无需分离,可直接对市售饮料中日落黄和柠檬黄含量进行同时测定,操作简单、实验成本低、结果准确。将该方法应用到仪器分析开放性实验教学中,有利于激发学生的科研兴趣,提高学生的科研技能及创新实践能力。

关键词：吸光度加和性仪器分析实验紫外—可见光谱法日落黄柠檬黄

SiO_2-螺旋纳米碳纤维双相炭黑的制备及结合胶性能研究

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化工新型材料 2018年第1期46卷 133-135,139页

作者：罗少伶陈建代祖洋聂松四川理工学院材料科学与工程学院自贡643000 材料腐蚀与防护四川省重点实验室自贡643000

双相炭黑是新型的填料,螺旋纳米碳纤维(HCNF)是具有特殊结构的炭材料。以气相生长HCNF为基础,通过原位生成法制备了双向炭黑——SiO_2/螺旋纳米碳纤维(SiO_2-HCNF),研究了SiO_2-HCNF的制备与性质。最后与炭黑N330和HCNF进行结合胶性能... 详细信息

双相炭黑是新型的填料,螺旋纳米碳纤维(HCNF)是具有特殊结构的炭材料。以气相生长HCNF为基础,通过原位生成法制备了双向炭黑——SiO_2/螺旋纳米碳纤维(SiO_2-HCNF),研究了SiO_2-HCNF的制备与性质。最后与炭黑N330和HCNF进行结合胶性能对比。结果表明:SiO_2-HCNF结合胶的抗拉强度较天然橡胶(NR)提高了0.2MPa,较HCNF结合胶提高了0.098MPa,并且结合胶量也提高了8.27%。

关键词：螺旋纳米碳纤维双相炭黑结合胶

玻璃纤维布/聚偏氟乙烯复合膜的制备与性能研究

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化工新型材料 2016年第12期44卷 94-96页

作者：罗少伶陈建何刚聂松代祖洋四川理工学院材料科学与工程学院自贡643000 材料腐蚀与防护四川省重点实验室自贡643000

通过在玻璃纤维布表面涂覆聚偏氟乙烯/硅烷偶联剂(PVDF/KH550)(质量比9∶1)粘接剂,将无机玻璃纤维与有机PVDF纤维进行复合,制得玻璃纤维/PVDF复合膜。将不同浓度(8%(wt,质量分数,下同)、10%、12%、14%)的涂覆液分别制备复合膜,表征复合... 详细信息

通过在玻璃纤维布表面涂覆聚偏氟乙烯/硅烷偶联剂(PVDF/KH550)(质量比9∶1)粘接剂,将无机玻璃纤维与有机PVDF纤维进行复合,制得玻璃纤维/PVDF复合膜。将不同浓度(8%(wt,质量分数,下同)、10%、12%、14%)的涂覆液分别制备复合膜,表征复合膜表面形貌、抗拉强度、吸液率、孔隙率和离子电导率、电化学稳定性和锂电池放电比容量。对比得出最佳涂覆液浓度10%,此时抗拉强度为35.70MPa,吸液率为177.65%,孔隙率为45.07%,锂离子传导率为0.2mS/cm。首次放电比容量为136mAh/g,在5C下复合膜的放电比容量比商用隔膜Celgard 2400高出47.3%。

关键词：玻璃纤维布/聚偏氟乙烯复合膜隔膜静电纺丝锂电池

静电纺丝制备PMMA/PVDF锂离子电池隔膜

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化工新型材料 2016年第10期44卷 64-66,69页

作者：胥会陈建代文超罗少伶四川理工学院材料腐蚀与防护四川省重点实验室自贡643000

利用电纺法制备了聚偏氟乙烯(PVDF)以及PVDF/聚甲基丙烯酸甲酯(PMMA)纳米纤维膜,用扫描电镜、红外光谱和热重等手段对纤维膜进行结构和性能表征,并测定了膜的孔隙率和吸液率。结果表明:当电压为20kV,接受距离为16cm,纺丝速率为0.001mm/s... 详细信息

利用电纺法制备了聚偏氟乙烯(PVDF)以及PVDF/聚甲基丙烯酸甲酯(PMMA)纳米纤维膜,用扫描电镜、红外光谱和热重等手段对纤维膜进行结构和性能表征,并测定了膜的孔隙率和吸液率。结果表明:当电压为20kV,接受距离为16cm,纺丝速率为0.001mm/s时,纺丝浓度为12%(质量分数),得到的纤维形貌较好。混合纺时PMMA加入没有改变PVDF原有的结构,复合隔膜的耐热温度达到470℃。PMMA/PVDF复合隔膜的吸液率和孔隙率整体提高且远远大于商业隔膜,其中PMMA/PVDF(4∶6)隔膜的吸液率和孔隙率分别达637%、67%,为最优。

关键词：静电纺丝聚偏氟乙烯聚甲基丙烯酸甲酯热性能空隙、吸液率