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Differentially.expressed gene in osteosarcoma cell lines with different metastatic potentials

Journal of Nanjing Medical University 2009年第5期23卷 352-358页

作者： Xinzhi Li Lin Meng Anming Chen Fengjin Guo zhenqiang Luo Heng Zeng Department of Orthopaedics Renhe Hospital Three Gorges University Yichang 443001 China Department of Orthopaedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Objective： To study.the expression of osteosarcoma metastasis associated gene using a cDNA microarray. and screen new candidate genes related＇to the development, progress and osteosarcoma metastasis. Methods： Total RNA of a low metastatic osteosarcoma and a high metastatic osteosarcoma （M6 and M8 cell lines, respectively. was extracted, purified to mRNA and then reverse transcribed to cDNA. M6 was used as the experimental group and M8 as the control group, and the gene expression of cells from both of these two sublines was investigated using cDNA microarray. containig 8064 cDNA clones. The cDNA of M6 was labeled with cy. and the cDNA of M8 was labeled with cy.. The two sublines were hy.ridized with the cDNA microarray. The hy.ridization signals were scanned with a Generation HI array.scanner and analy.ed by.Imagequant 5.0 software. Results： There were 330 differentially.expressed genes between M6 and M8. In the M6 subline,152 genes were up-regulated and 178 genes were down-regulated compared to the M8 subline. These genes could be classified according to their function. Cell growth-related genes that were down-regulated included CCNG1, CDC2, APC10,and RPA3, while expression of the tumor suppressor genes, CDKN1A and CDKN2D, was up-regulated. Other genes that were differentially.expressed included those that have been implicated in the regulation of signal transduction, metabolism and apoptosis. Conclusion： This study.exploits a cDNA microarray.approach to identify.ng genes that may.be associated with metastasis. The gene expression profiles of osteosarcoma cell lines is a potentially.important index in the search of new candidate genes related to tumor occurrence, development and metastasis.

关键词： Osteosarcoma Gene expression cDNA microarray

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Heavy.color Fine Brush Painting of Ancient China

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China & The World Cultural Exchange 2011年第5期77卷 12-15页

作者： y.n zhenqiang

The Six Principles of Chinese Painting was established by.Xie He, an art historian and critic in 6th-century.China. The six points to consider when judging a painting are: "spirit resonance"

关键词：古代中国中国画美术通信对象艺术

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Love Makes Their Life Worth——Story.about Shao Junke's help for the handicapped

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人权 2008年第2期 24-25页

作者： ZHU zhenqiang

The handicapped,a special group in society.usually.encounter many.difficulties in employ. ment,marriage,and daily.life. Some difficulties are what they.cannot conquer by.*** is also not easy.for them to integrate into ***- er,if there is a person who helps the hand- icapped find a job,build up confidence and make their life worth;if there is a person who helps them set up their families and incorporate in a harmonious community. and if there is a person who takes care of the children with disabilities just as their owns;how noble this person is!

关键词： work Story.about Shao Junke’s help for the handicapped Love Makes Their Life Worth

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巨噬细胞迁移抑制因子对人胚胎干细胞存活、增殖和分化的影响

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中国组织工程研究 2025年第7期29卷 1380-1387页

作者：黄婷郑晓晗钟远吉魏艳召魏绪芳曹旭东冯晓丽赵振强海南医学院第一附属医院神经内科海南省海口市570100 海南医学院海南省海口市570100 海南省热带脑科学研究与转化重点实验室海南省海口市570100 渥太华大学加拿大渥太华K1N6N5

背景:巨噬细胞迁移抑制因子(macrophage migration inhibitory.factor,MIF)是一种具有多效性作用的细胞因子,可以在不同类型干细胞中自分泌并且能调控细胞的增殖、分化和迁移。课题组前期研究证实人胚胎干细胞自分泌MIF,且在培养液中浓... 详细信息

背景:巨噬细胞迁移抑制因子(macrophage migration inhibitory.factor,MIF)是一种具有多效性作用的细胞因子,可以在不同类型干细胞中自分泌并且能调控细胞的增殖、分化和迁移。课题组前期研究证实人胚胎干细胞自分泌MIF,且在培养液中浓度基本固定。然而,MIF是否参与了人胚胎干细胞的存活、增殖和分化尚不清楚。目的:探究MIF对人胚胎干细胞存活、增殖和分化的作用。方法:(1)培养人胚胎干细胞H9,CCK-8法检测并绘制细胞生长曲线,采用酶联免疫吸附法定量检测培养基中MIF水平。(2)为了明确外源性MIF对人胚胎干细胞存活、增殖的影响,分为:对照组,细胞在干细胞培养基中正常培养;外源性MIF组,在干细胞培养基中分别添加30,100,300 ng/m L的MIF;MIF抑制剂ISO-1组,在干细胞培养基中分别添加2,7,21μmol/L的ISO-1;MIF+ISO-1组,在不同浓度ISO-1组中分别添加100 ng/m L MIF,采用CCK-8法检测上述各组细胞活力。(3)为进一步阐明MIF基因对人胚胎干细胞存活、增殖的影响,采用CRISPRCas9技术构建MIF敲除的H9细胞系,观察建系情况。(4)为了明确高浓度MIF对人胚胎干细胞初步分化是否有影响,在培养基中分别添加100 ng/m L MIF和100 ng/m L CXCR4中和抗体,采用实时荧光定量聚合酶链式反应(RT-q PCR)、免疫细胞荧光、蛋白质印迹法(Western blot)检测干细胞自我更新因子(KLF4、c-My.、NANOG、OCT4、SOX2)及分化转录因子(FOXA2、OTX2)的表达水平。结果与结论:(1)人胚胎干细胞H9的对数生长期为3-6 d,正常生长的情况下自分泌MIF水平约为20 ng/m L,与细胞量无关;(2)与对照组相比,添加不同质量浓度MIF对人胚胎干细胞的增殖无影响(P>0.05);ISO-1明显抑制人胚胎干细胞的增殖,ISO-1浓度越大,抑制越明显(P<0.05);ISO-1中添加MIF可以减少ISO-1的抑制作用(P<0.05);(3)RT-q PCR检测MIF基因敲除约50%后,人胚胎干细胞生长活力显著降低并且无法建系成功;(4)在培养基中添加100 ng/m L外源性MIF,自我更新转录因子KLF4的m RNA、蛋白及荧光表达水平均下降;分化因子FOXA2的m RNA、蛋白及荧光表达水平均上升;(5)在培养基中添加100 ng/m L CXCR4中和抗体,KLF4的m RNA及蛋白表达水平均上升;FOXA2的m RNA及蛋白表达水平均下降,与MIF组表达趋势相反。综上所述,人胚胎干细胞自分泌的MIF是其存活所必需的;培养基中额外添加MIF并不能促进人胚胎干细胞增殖,但可以使自我更新因子KLF4表达下降,转录因子FOXA2表达上升,为下一步探明MIF对人胚胎干细胞分化的影响及机制提供了线索,MIF-CXCR4轴在其中起到一定的调控作用。

关键词：巨噬细胞迁移抑制因子人胚胎干细胞自分泌存活分化 CXCR4 KLF4 FOXA2

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4-Formy.-2-(2H-benzotriazol-2-y.)-phenol: an ESIPT chromophore

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Chinese Science Bulletin 2014年第34期59卷 4741-4751页

作者： y.o Lu y.long Gong Huiy.ng Ma zhenqiang Wang Haili Li Ziping Luo Hongru Li Fang Gao School of Chemistry.and Chemical Engineering Chongqing University

In the present work, excited state intramolecular proton transfer(ESIPT) emission properties of a new benzotriazole derivative 4-formy.-2-(2H-benzotriazol-2-y.)-phenol(C1) were studied. 4-Formy.-2-(2H-benzotriazol-2-y.)-methoxy.benzene(C2), 4-formy.-2-(2H-benzotriazol-2-y.)-pheny. acetate(C3) and 4-methy.-2-(2H-benzotriazol-2-y.)-phenol(C4) were used as the reference molecules.1H NMR chemical shift of hy.roxy.group in C1 was located at more down field than that of C4 or p-hy.roxy.benzaldehy.e(C5), respectively. C1 showed two absorption bands in the range of 260–400 nm zones in various solvents, while C2 and C3 exhibited single absorption band. The equal molar mixtures of C2/C5 or C3/C5 showed single absorption band.C2 and C3 display.d single fluorescence emission band in various solvents, while C1 exhibited dual emission bands in some strong polar solvents. Furthermore, the second emission band in these strong polar solvents showed the large Stokes shift. The results show that the second emission band of C1 was produced by.ESIPT. C2 and C3 could not undergo ESIPT due to no hy.roxy.group. The geometry.optimization calculation of enol and keto forms in the ground and excited states of C1 provided tough theoretical evidences of ESIPT.

关键词：苯并三唑甲酰基激发态分子内质子转移苯酚极性溶剂生色发射性能三唑衍生物

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Anshun’s Nuo Opera:a Living Fossil of Drama

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China & The World Cultural Exchange 2010年第7期76卷 38-40页

作者： y.n zhenqiang

Nuo dance,or Nuo opera,is an indigenous dramatic art evolved from ancient religious rites. Nuo opera,deemed the"living fossil of opera,"

关键词：活化石戏剧安顺中国西南地区宗教仪式使用性能 amp 特色化

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Love Makes Their Life Worth——Story.about Shao Junke's help for the handicapped

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The Journal of Human Rights 2008年第2期7卷 24-25页

作者： ZHU zhenqiang

The handicapped, a special group in society. usually.encounter many.difficulties in employ.ent, marriage, and daily.*** difficulties are what they.cannot conquer by.themselves. It is also not easy.for them to integrate into society. However, if there is a person who helps the hand- icapped find a job, build up confidence and make their life worth; if there is a person who helps them set up their families and incorporate in a harmonious community.

关键词： work Story.about Shao Junke’s help for the handicapped Love Makes Their Life Worth

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Feed conversion ratio,residual feed intake and cholecy.tokinin ty.e A receptor gene poly.orphisms are associated with feed intake and average daily.gain in a Chinese local chicken population

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Journal of Animal Science and Biotechnology 2018年第4期9卷 794-802页

作者： Zhenhua y. Xing Li Wen Luo zhenqiang Xu Congliang Ji y.n Zhang Qinghua Nie Dexiang Zhang Xiquan Zhang Department of Animal Genetics Breeding and ReproductionCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Provincial Key.Lab of Agro-Animal Genomics and Molecular Breeding and Key.Laboratory.of Chicken Genetics Breeding and ReproductionMinistry of AgricultureGuangzhou 510642GuangdongChina Wen’s Nanfang Poultry.Breeding Co.Ltd Yunfu 527400GuangdongChina

Background: The feed conversion ratio(FCR) and residual feed intake(RFI) are common indexes in measuring feed efficiency.for livestock. RFI is a feed intake adjusted for requirements for maintenance and production so these two traits are related. Similarly. FCR is related to feed intake and weight gain because it is their ratio. Cholecy.tokinin ty.e A receptor(CCKAR) play. an important role in animal digestive process. We examined the interplay.of these three parameters in a local Chinese chicken ***: The feed intake(FI) and body.weights(BW) of 1,841 individuals were monitored on a daily.basis from 56 to 105 d of age. There was a strong correlation between RFI and average daily.feed intake(ADFI) and a negative correlation between the FCR and daily.gain(r=-0.710). Furthermore, we identified 51 single nucleotide poly.orphisms(SNPs) in the CCKAR and 4 of these resulted in amino acid mutations. The C334A mutation was specifically.associated with FI and the expected feed intake(EFI)(P y.associated with the average daily.gain(ADG)(P y.associated with FI and EFI(P y.to weight selecting, and RFI is more appropriate if the breeding focus is feed intake. And C334A and G1290A of the CCKAR gene can be deemed as candidate markers for feed intake and weight gain.

关键词： CCKAR Chicken FCR RFI SNP

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Feasibility.Study.and Application of V—Fe Alloy.Slag as Refractory.Material

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China's Refractories 1997年第2期6卷 17-21页

作者： zhenqiang XIAOJun 等 PangangRefractory.o. Td.PanzhihuaSichuanChina BeijingPoly.echnieUniversity BeijingChina

The possibility.of using V-Fe alloy.slag as refractory.rqaw material has been investigated,The V-Fe alloy.slag is examinated by.chemical analy.is ,petrographic analy.is and macroex-amination,It is found that the slag contains about 90% Al2O3,and corundum as main litho-facies,which i with high refractoriness (>1790℃) and high bulk density.>3.6 g/cm^3).Instead of extra pure bauxite,the V-Fe alloy.slag has been applied to refractory.brick as aggregate.

关键词：耐火材料钒铁 V Fe 合金炉渣

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Research on Talent Training in Vocational Education Based on Craftsman Spirit

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教育研究前沿（中英文版） 2022年第1期12卷 62-65页

作者： zhenqiang Zhao School of Applied Foreign Languages Zhejiang Yuexiu UniversityShaoxingZhejiang312000China

Craftsman spirit is a professional attitude and spiritual concept,a value orientation and behavior expression of employ.es,and also the spiritual quality.of craftsmen who pursue perfection,keep improving and constantly.*** craftsman spirit is the need of the development of the times,the reform of vocational education and the growth of *** stage of higher vocational education is the key.period for students to understand,and master the craftsman *** colleges should integrate the craftsman spirit into the whole process of talent training,take multiple measures at the same time,and improve the effectiveness of talent training.

关键词： Craftsman Spirit Vocational Education Personnel Training

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