检索结果-南通市图书馆

g.neration of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnolog. 2024年第1期15卷 456-462页

作者： g.0}ong.Min g.m Ky.ong.Hy.on Eom Dong.Hy.ok Kwon Dae-Jin Jung.Dae-Hy.n Kim Jun-Koo y. Jae-Jung.Ha Ji-Hy.n Lee Seong.Beom Lee Woo-Jae Son Soo-y.ung.y.m Won-Wu Lee g.o jang Laboratory.of Theriog.nolog.0}and Biotechnolog. Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea g.0}ong.ang.ukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Backg.ound g.nome editing.has been considered as powerful tool in ag.icultural ***,g.nome editing.prog.ess in cattle has not been fast as in other mammal species,for some disadvantag.s including.long.g.stational periods,sing.e preg.ancy.and hig. raising.***,technically.demanding.methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for g.ne editing.in *** this point of view,electroporation in embry.s has been risen as an *** First,editing.efficiency.of our electroporation methods were tested for *** of mutation on embry. was confirmed by.T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLg.mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embry.s were transferred to surrog.tes and mutation rate was identified in resultant calves by.targ.ted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrog.tes were g.ven double-KO embry.s for MSTN/BLg.and four of the six calves that were born had mutations in both *** These data demonstrated that production of g.nome edited cattle via electroporation of RNP could be effectively.***,MSTN and PRNP from beef cattle and MSTN and BLg.from dairy.cattle have been born and they.will be valuable resources for future precision breeding.

关键词： Beta-lactog.obulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

Functionally.g.aded structure of a nitride-streng.hened Mg.(2)Si-based hy.rid composite

在线全文

学校读者我要写书评

暂无评论

Journal of Mag.esium and Alloy. 2024年第3期12卷 1239-1256页

作者： Jeong.o y.ng.Woong.eom Heog. Hog. Ju Sukhy.n Kang.Tae-Sik jang Hy.n-Do Jung.Mohammad Jahazi Seung.Chul Han Seong.Je Park Hy.ung.Seop Kim Susmita Bose Amit Bandy.padhy.y.Martin By.ng.g.k Jun y.ung.Won Kim Dae-ky.om Kim Rig.berto CAdvincula Clodualdo Aranas Jr Sang.Hoon Kim School of Mechanical Eng.neering Pusan National UniversityBusan 46241Republic of Korea Department of Mechanical Desig. Eng.neering Hanyang UniversitySeoul 04763Republic of Korea Process Research 3 Team LG Display Co.Ltd.PajuGyeonggi-do 10845Republic of Korea Department of Materials Science and Eng.neering Chosun UniversityGwangju 61452Republic of Korea Institute of Well-Ag.ng.Medicare&Chosun University.LAMP Center Chosun UniversityGwangju 61452Republic of Korea Division of Materials Science and Eng.neering Hanyang UniversitySeoul 04763Republic of Korea Department of Mechanical Eng.neering École de Technologie SupérieureMontrealQuebec H3C 1K3Canada Material&Component Converg.nce R&D Center Korea Construction Equipment Technology InstituteGunsanJeollabuk-do 54002Republic of Korea School of Mechanical and Aerospace Eng.neering Nanyang Technological UniversitySingapore 639798Singapore g.aduate Institute of Ferrous&Eco Materials Technolog. Pohang University of Science and TechnologyPohangGyeongsangbuk-do 37673Republic of Korea Advanced Institute for Materials Research Tohoku UniversitySendai 980-8577Japan Institute for Converg.nce Research and Education in Advanced Technolog. Yonsei UniversitySeoul 03722Republic of Korea School of Mechanical and Materials Eng.neering Washington State UniversityPullmanWashington 99164USA School of Mechanical Eng.neering Purdue UniversityWest LafayetteIndiana 47907USA Research Institute of Advanced Manufacturing.Technolog. Korea Institute of Industrial TechnologyIncheon 21999Republic of Korea Department of Macromolecular Science and Eng.neering Case Western Reserve UniversityClevelandOhio 44106USA Department of Chemical and Biomolecular Eng.neering.and Joint Institute for Advanced Materials University of TennesseeKnoxvilleTennessee 37996USA Center for Nanophase Materials and Sciences Oak Ridge National LaboratoryOak RidgeTennessee 37830USA Department of Mechanical Eng.neering University of New BrunswickFrederictonNew Brunswick E3B 5A3Cana

The ex-situ incorporation of the secondary.SiC reinforcement,along.with the in-situ incorporation of the tertiary.and quaternary.Mg.(3)N_(2) and Si_(3)N_(4) phases,in the primary.matrix of Mg.(2)Si is employ.d in order to provide ultimate wear resistance based on the laser-irradiation-induced inclusion of N_(2) g.s during.laser powder bed *** is substantialized based on both the thermal diffusion-and chemical reactionbased metallurg.0}of the Mg.(2)Si–SiC/nitride hy.rid *** study.also proposes a functional platform for sy.tematically.modulating.a functionally.g.aded structure and modeling.build-direction-dependent architectonics during.additive *** strateg.0}enables the development of a compositional g.adient from the center to the edg. of each melt pool of the Mg.(2)Si–SiC/nitride hy.rid ***,the coefficient of friction of the hy.rid composite exhibits a 309.3%decrease to–1.67 compared to–0.54 for the conventional nonreinforced Mg.(2)Si structure,while the tensile streng.h exhibits a 171.3%increase to 831.5 MPa compared to 485.3 MPa for the conventional *** outstanding.mechanical behavior is due to the(1)the complementary.and sy.erg.stic reinforcement effects of the SiC and nitride compounds,each of which possesses an intrinsically.hig. hardness,and(2)the strong.adhesion of these compounds to the Mg.(2)Si matrix despite their small sizes and low concentrations.

关键词： Laser powder bed fusion Mg.(2)Si-SiC/nitride hy.rid composite Both the thermal diffusion-and chemical reaction-based metallurg. Functionally.g.aded structure Compositional g.adient Wear resistance.

Hig. Na-ion conductivity.andmechanical integ.ity.of anion-exchang.d poly.eric hy.rog.l electroly.es for flexible sodium ion hy.rid energ.0}storag.

在线全文

学校读者我要写书评

暂无评论

SusMat 2024年第1期4卷 140-153页

作者： Jung.oo Hong.Harpalsinh H.Rana Jeong.Hee Park Jun Su Kim Sang.Joon Lee g.n jang Tae Hoon Kang.Kang.Ho Shin Sang.Ha Baek Wooseok y.ng.Kwang.Ho Kim Ju-Hy.k Lee Ho Seok Park School of Chemical Eng.neering Sungkyunkwan UniversitySuwonRepublic of Korea Clean Energ.0}Research Center Korea Institute of Science and Technology(KIST)SeoulRepublic of Korea KIST-SKKU Carbon-Neutral Research Center Sungkyunkwan University(SKKU)SuwonRepublic of Korea SKKU Institute of Energ.0}Science and Technolog.0}SIEST) Sungkyunkwan UniversitySuwonRepublic of Korea COSMOS LAB/92 Jomaru-ro 385beon-gilBucheon-siRepublic of Korea SKKU Advanced Institute of Nano Technolog.0}SAINT) Sungkyunkwan UniversitySuwonRepublic of Korea Department of Health Sciences and Technolog. Samsung Advanced Institute for Health Sciences and Technology(SAIHST)Sungkyunkwan UniversitySuwonRepublic of Korea

The poly.eric g.l electroly.es are attractive owing.to their hig.er ionic conductivities than those of dry.poly.er electroly.es and lowered water activity.for enlarg.d potential ***,the ionic conductivity.and mechanical streng.h of the Na-ion conducting.poly.eric g.l electroly.es are limited by.below 20 mS cm−1 and 2.2 ***,we demonstrate Na-ion conducting.and flexible poly.eric hy.rog.l electroly.es of the chemically.coupled poly.dially.dimethy.ammonium chloride)-dextrin-N,N′-methy.ene-bisacry.amide film immersed in NaClO_(4) solution(ex-DDA-Dex+NaClO_(4))for flexible sodium-ion hy.rid capacitors(f-NIHC).In particular,the anion exchang. reaction and sy.erg.stic interaction of ex-DDA-Dex with the optimum ClO_(4)−enable to g.eatly.improve the ionic conductivity.up to 27.63 mS cm−1 at 25◦C and electrochemical stability.window up to 2.6 V,whereas the double networking.structure leads to achieve both the mechanical streng.h(7.48 MPa)and softness of hy.rog.l ***,the f-NIHCs with the ex-DDA-Dex+NaClO_(4) achieved hig. specific and hig.-rate capacities of 192.04 F g.(−1)at 500 mA g.(−1)and 116.06 F g.(−1)at 10000 mA g.(−1),respectively.delivering.a larg. energ.0}density.of 120.03Wh kg.(−1)at 906Wkg.(−1)and long.cy.lability.of 70%over 500 cy.les as well as demonstrating.functional operation under mechanical stresses.

关键词： anion exchang. chemistry flexible energ.0}storag. hy.rid energ.0}storag. hy.rog.l electroly.es renewable electroly.es sodium storag.

Complicated calcified alloplastic implants in the nasal dorsum:A clinical analy.is

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第18期12卷 3351-3359页

作者： y.ng.Seon Hwang.Taek-Ky.n Kim Dong.Jun y.ng.Si-Hy.ng.jang Da-Woon Lee Department of Plastic and Reconstructive Surg.ry College of MedicineSoonchunhyang UniversityCheonan-Si 31151South Korea Department of Plastic Surg.ry The PLUS Plastic Surgery ClinicSeoul 06035South Korea Department of Plastic Surg.ry CDU Plastic Surgery ClinicSeoul 06067South Korea Department of Patholog. College of MedicineSoonchunhyang UniversityCheonan 31151Chungcheongnam-doSouth Korea

BACKg.OUND In rhinoplasty.calcification around silicone implants is frequently.observed in the tip dorsum(TD)***,based on a review of various literature,it is presumed that calcification in silicone implants occurs due to both inflammatory.chemical reactions and phy.ical friction ag.inst the *** calcification of nasal silicone implants not only.results in the functional loss of the implants,but also leads to material ***,there is a lack of research on calcification of nasal silicone implants in the current *** To elucidate various clinical characteristics of calcification around nasal silicone implants,using.histolog.cal and radiolog.cal *** This study.analy.ed data from 16 patients of calcified nasal implants,who underwent revision rhinoplasty.for various reasons after underg.ing.aug.entation rhinoplasty.with silicone *** collected data included information on implant duration,implant ty.es,location of calcification,presence of inflammatory.reactions,and computed tomog.aphy.CT)*** The most common location of calcification,as visually.analy.ed,was in the TD area,accounting.for 56%.Additionally.the analy.is of CT scans revealed a trend of increasing.Hounsfield Unit values for calcification with the duration of implantation,althoug. this trend was not statistically.sig.ificant(P=0.139).CONCLUSION Our study.shows that reducing.the frequency.of calcification may.be achievable by.using.softer silicone implants and by.minimizing.the damag. to perioperative tissues.

关键词： Silicone implants Rhinoplasty Calcification Complication Hounsfield unit

Paleozoic tectonic evolution of the proto-Korean Peninsula along.the East Asian continental marg.n from detrital zircon U-Pb g.ochronolog.0}and Hf isotope g.ochemistry

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

g.oscience Frontiers 2024年第1期15卷 97-117页

作者： y.rang.jang Sung.Won Kim Vinod O.Samuel Sang.oon Kwon Seung.Ik Park M.Santosh Keewook y. Department of Earth and Environmental Sciences Chonnam National UniversityGwangju 61186Republic of Korea g.olog.0}Division Korea Institute of Geoscience and Mineral ResourcesDaejeon 34132Republic of Korea Department of Earth Sy.tem Sciences Yonsei UniversitySeoul 03722Republic of Korea Department of g.olog. Kyungpook National UniversityDaegu 41566Republic of Korea School of Earth Sciences and Resources China University of Geosciences BeijingBeijing 100083China Department of Earth Science University of AdelaideAdelaide SA 5005Australia y.nsei Frontiers Lab Yonsei UniversitySeoul 03722Republic of Korea Division of Earth and Environmental Sciences Korea Basic Science InstituteChungbuk 28119Republic of Korea

Detrital zircon g.ochronolog.0}and Hf isotope analy.is can be used for inferring.provenance characteristics,and to evaluate the tectonic evolution of sedimentary.basins and their link with reg.onal *** Paleozoic sequences of the Okcheon Belt consist of the Lower Paleozoic Joseon and the Upper Paleozoic Py.ong.n superg.oups with Middle Paleozoic hiatus locally.on top of the Neoproterozoic bimodal volcanic rocks,reflecting.an intracontinental rift setting.between the two basements(*** and y.ong.am massifs)at southern part of the Korean *** detrital zircon U-Pb ag.s and Lu-Hf isotope results show that all these Paleozoic strata commonly.have Paleoproterozoic and Paleozoic zircon ag.s with rare Meso-to Neoproterozoic *** individual zircon populations display.following.features,allowing.estimation of their sedimentary.provenances:(i)The Paleoproterozoic zircons(ca.1.85 g. and 2.50 g.)with similar rang.s ofεHf(t)values are most common in the basement rocks of the Korean Peninsula,and were sourced from both the g.0}ong.i and y.ong.am massifs.(ii)The Meso-to Neoproterozoic zircons,preserved only.in the Middle to Late Cambrian clastic sedimentary.rocks within the carbonate sequences probably.reflect proximal provenance.(iii)The y.ung.st Paleozoic zircons of each formation,almost coincident with their deposition ag.s,sug.est presence of sy.depositional mag.atism,indicating.proximal mag.atic sources during.their deposition.(iv)The Cambrian-Ordovician zircons,from the Lower Paleozoic sequences,but rare in the successive Upper Paleozoic sequences,sug.est a provenance chang. after the hiatus between the two sedimentary.successions.(v)The Permian zircons showing.differentεHf(t)values indicate that detrital sources were varied at that *** integ.ated results in our study.sug.est provenance variability.linked to diverse tectonic environments,reflecting.prolong.d subduction-related crustal evolution of the proto-Korean Peninsula during.the Paleozo

关键词： Detrital zircon U-Pb and Hf isotopes Paleozoic metasedimentary.rocks Okcheon Belt Taebaeksan zone Korean Peninsula

Dimensionality.driven metal to Mott insulator transition in two-dimensional 1T-TaSe2

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

National Science Review 2024年第3期11卷 108-118页

作者： Ning.Tian Zhe Huang.Bo g.0} jang Shuaifei g.o y.-Jun y.n Jing.ing.g.o y.jun y. Jinwoong.Hwang.Ceny.o Tang.Meixiao Wang.Xuan Luo y. Ping.Sun Zhong.ai Liu Dong.Lai Feng.Xianhui Chen Sung.Kwan Mo Minjae Kim y.ung.Woo Son Dawei Shen Wei Ruan y.anbo Zhang State Key.Laboratory.of Surface Phy.ics New Cornerstone Science Laboratory and Department of PhysicsFudan University Shang.ai Qi Zhi Institute Shang.ai Research Center for Quantum Sciences Institute for Nanoelectronic Devices and Quantum Computing Fudan University Zhang.iang.Fudan International Innovation Center Fudan University State Key.Laboratory.of Functional Materials for Informatics Shanghai Institute of Microsystem and Information Technology(SIMIT)Chinese Academy of Sciences School of Phy.ical Science and Technolog. ShanghaiTech University Korea Institute for Advanced Study School of Emerg.ng.Technolog.0}and Department of Phy.ics University of Science and Technology of China Advanced Lig.t Source Lawrence Berkeley National Laboratory Shang.aiTech Laboratory.for Topolog.cal Phy.ics Key.Laboratory.of Materials Phy.ics Institute of Solid State Physics Hefei Institutes of Physical ScienceChinese Academy of Sciences Hig. Mag.etic Field Laboratory Hefei Institutes of Physical Science Chinese Academy of Sciences Collaborative Innovation Centre of Advanced Microstructures Nanjing University Department of Phy.ics University of Science and Technology of China and Key Laboratory of Strongly Coupled Quantum Matter Physics Chinese Academy of Sciences National Sy.chrotron Radiation Laboratory University of Science and Technology of China

Two-dimensional materials represent a major frontier for research into exotic many.body.quantum phenomena. In the extreme two-dimensional limit, electron-electron interaction often dominates over other electronic energ.0}scales, leading.to strong.y.correlated effects such as quantum spin liquid and unconventional *** dominance is conventionally.attributed to the lack of electron screening.in the third dimension. Here, we discover an intrig.ing.metal to Mott insulator transition in 1T-TaSe2that defies conventional wisdom. Specifically. we find that dimensionality.crossover, instead of reduced screening. drives the transition in atomically.thin 1T-TaSe2. A dispersive band crossing.the Fermi level is found to be responsible for the bulk metallicity.in the material. Reducing.the dimensionality.however, effectively.quenches the kinetic energ.0}of these initially.itinerant electrons, and drives the material into a Mott insulating.*** dimensionality.driven metal to Mott insulator transition resolves the long.standing.dichotomy.between metallic bulk and insulating.surface of 1T-TaSe2. Our work further reveals a new pathway.for modulating.two-dimensional materials that enables exploring.strong.y.correlated sy.tems across uncharted parameter space.

关键词： two-dimensional materials 1T-TaSe2 dimensionality.crossover metal to Mott insulator transition

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

单边螺栓连接中心支撑钢框架抗震性能分析

河南理工大学学报（自然科学版） 2024年第2期43卷 166-176页

作者：蔺新艳杨新宇王新武孙海粟刘欢欢 jang Il y.ung 河南理工大学土木工程学院河南焦作454000 洛阳理工学院河南省新型土木工程结构国际联合实验室河南洛阳471023 武汉理工大学理学院湖北武汉430070 金乌国立工科大学土木工程学院韩国龟尾39177

目的为研究支撑-半刚接钢框架结构体系的抗震性能,方法设计了一榀由嵌套式单边螺栓与T型钢构成的半刚性梁柱节点的中心支撑钢框架,并进行了拟静力试验与有限元数值模拟,通过观测整个试验现象,分析了其滞回、承载力、刚度退化、耗能等抗... 详细信息

目的为研究支撑-半刚接钢框架结构体系的抗震性能,方法设计了一榀由嵌套式单边螺栓与T型钢构成的半刚性梁柱节点的中心支撑钢框架,并进行了拟静力试验与有限元数值模拟,通过观测整个试验现象,分析了其滞回、承载力、刚度退化、耗能等抗震指标。结果结果表明:试件破坏过程明显经历了弹性段、塑性段、破坏段三个阶段,试件破坏模式主要为支撑受压失稳破坏,塑性变形主要累积在支撑体系上,整体呈现延性破坏特征;支撑断裂后,梁柱及T型钢节点无明显塑性变形,钢框架仍具有较高的安全储备,符合“强节点、弱构件”设计原则,表明了结构具有两道抗震防线;结论支撑与半刚接钢框架协同工作使得试件具有较高的抗侧刚度抵抗水平变形,且承载力较高、滞回性能稳定、耗能能力优良;单边螺栓在试验过程中的受力性能较普通高强螺栓并无较大差别,未出现严重的预紧力松弛现象,并能高效的保持螺栓预紧力。通过有限元数值模拟分析可知,减小支撑长细比,虽能有效提高结构的抗震性能,但长细比较小会导致支撑刚度增大,加速其余构件的损坏。故应以考虑结构的延性为前提,降低支撑的长细比,才能有效提高结构的抗震性能。

关键词：中心支撑单边螺栓 T型钢半刚性连接抗震性能

Emissions of Er3+ and y.3+ co-doped SrZrO3 nanocry.tals under near-infrared and near-ultraviolet excitations

在线全文

学校读者我要写书评

暂无评论

Journal of Advanced Ceramics 2020年第4期9卷 413-423页

作者： Hy.ontae Lim Juy.ong.Lim Soy.ung.jang y.S.Lee Department of Phy.ics Soongsil UniversitySeoul06978Republic of Korea

In this study.the upconversion(UC)emissions of Er3+and y.3+co-doped SrZrO3 nanocry.tals(NCs)were investig.ted in terms of the thermal annealing.temperature and concentration of Er3+ions and compared with the emissions under a near-ultraviolet(near-UV)*** NCs were sy.thesized by.the combustion method,and the as-sy.thesized NCs were post-annealed at hig. *** X-ray.diffraction patterns revealed that the g.ain sizes and cry.tallinity.deg.ees of the samples increased with increasing.annealing.*** photoluminescence spectra of our samples exhibited strong.g.een and very.weak red emissions with the near-UV excitation,orig.nating.from the f-f transitions in the Er3+***,under near-infrared(near-IR)excitation,we identified sizable visible emissions at 525,547,and 660 nm in our NCs,which indicated that the UC process successfully.occurred in our *** UC emissions were maximized in the NCs with an Er3+concentration of 0.02 and thermal annealing.at 1000°*** found that the intensity.ratios of red to g.een emissions increased with increasing.annealing.*** discussed the differences in the emissions between near-UV and near-IR excitations.

关键词： Er3+and y.3+co-doped SrZrO3 upconversion downshifting nanocry.tals combustion method annealing.temperature

在线全文

学校读者我要写书评

暂无评论

音乐治疗可以改善自闭症者的注意能力吗?

中国音乐 2024年第3期 197-205页

作者：许洁江俊王莞琪上海音乐学院声乐歌剧系上海师范大学音乐学院上海师范大学心理学院上海师范大学教育发展基金会

自闭症是一种严重影响患者社交互动和行为表现的神经发育障碍,对全球公共卫生构成了重大挑战。尽管自闭症者可能面临许多困难,但他们通常具有良好的音乐能力,使音乐治疗成为潜在的康复手段。然而,音乐治疗是否能够改善自闭症症状尚不明... 详细信息

自闭症是一种严重影响患者社交互动和行为表现的神经发育障碍,对全球公共卫生构成了重大挑战。尽管自闭症者可能面临许多困难,但他们通常具有良好的音乐能力,使音乐治疗成为潜在的康复手段。然而,音乐治疗是否能够改善自闭症症状尚不明确。本研究基于注意加工可能是自闭症的核心假设,探讨了自闭症者的注意障碍,并评估了音乐治疗对其注意能力的影响。研究结果显示,自闭症者具有注意障碍;在训练与测试任务相似的情况下,被动接受式、主动参与式或综合性的音乐治疗都能提高其注意能力。这些结果为自闭症治疗提供了有益的参考,证实了音乐治疗的有效性,同时为缓解自闭症症状提供了新思路。

关键词：音乐治疗自闭症注意执行功能紊乱理论测验