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广州市某马术俱乐部马源大肠杆菌的分离鉴定、毒力基因检测及耐药性分析

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畜牧与饲料科学 2023年第3期44卷 109-115页

作者：刘鏊马丁允陀海欣李静王超男孙铭飞林栩慧齐萌塔里木大学动物科学与技术学院/新疆生产建设兵团塔里木动物疫病诊断与防控工程实验室新疆阿拉尔843300 广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室广东广州510640

[目的]分离鉴定广州市某马术俱乐部赛马粪便中的大肠杆菌,检测其毒力基因,并进行耐药性分析。[方法]收集广州市某马术俱乐部20份成年赛马粪便样本,通过细菌培养、革兰染色镜检和细菌16S rDNA序列PCR法分离鉴定大肠杆菌,并对分离菌株进... 详细信息

[目的]分离鉴定广州市某马术俱乐部赛马粪便中的大肠杆菌,检测其毒力基因,并进行耐药性分析。[方法]收集广州市某马术俱乐部20份成年赛马粪便样本,通过细菌培养、革兰染色镜检和细菌16S rDNA序列PCR法分离鉴定大肠杆菌,并对分离菌株进行大肠杆菌系统发育群鉴定;采用PCR法检测10种毒力基因和11种耐药基因,应用K-B纸片法开展药物敏感性试验。[结果]经形态学观察和PCR检测,鉴定出13株大肠杆菌,分离率为65.00%(13/20);系统进化分群分别属于A群(23.07%,3/13)、B1群(61.54%,8/13)和D群(15.38%,2/13);共检出5种毒力基因,分别为fimC(61.54%,8/13)、fyuA(7.69%,1/13)、irp2(7.69%,1/13)、stx1(15.38%,2/13)和stx2(7.69%,1/13);共检出9种耐药基因,分别为parC(100%,13/13)、gyrA(100%,13/13)、gyrB(92.31%,12/13)、sul2(76.92%,10/13)、cmlA(30.77%,4/13)、aadA(23.07%,3/13)、tet(B)(15.38%,2/13)、blaTEM(7.69%,1/13)和blaCTX(7.69%,1/13);13株大肠杆菌分离株对头孢西丁、庆大霉素、环丙沙星敏感,对四环素的耐药率为38.46%,对头孢噻呋、阿米卡星、氨苄西林的耐药率均为30.77%,对氯霉素、甲氧苄啶/磺胺异噁唑和亚胺培南也呈现出不同程度的耐药性。[结论]广州市某马术俱乐部赛马源大肠杆菌种群具有多样性,携带多种毒力基因和耐药基因,呈现多重耐药性,提示临床上应加强马大肠杆菌病的防治。

关键词：大肠杆菌系统进化群毒力基因耐药基因马

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中国碳收支:1980~2021年

science Bulletin 2024年第1期69卷 114-124页

作者：夏晓圣任培阳王旭辉刘丹陈修治丹利何斌何洪林居为民梁敏琪陆星劼彭静覃章才夏江周郑博魏静乐旭于贵瑞朴世龙袁文平 School of Atmospheric sciences Guangdong Province Data Center of Terrestrial and Marine Ecosystems Carbon CycleSun Yat-sen UniversityZhuhai 510245China Sino-French institute for Earth System science College of Urban and Environmental SciencesPeking UniversityBeijing 100091China State Key Laboratory of Tibetan Plateau Earth System Resources and EnvironmentInstitute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China Key Laboratory of Regional Climate-Environment for Temperate East Asia Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China State Key Laboratory of Earth Surface Processes and Resource Ecology College of Global Change and Earth System ScienceBeijing Normal UniversityBeijing 100875China Synthesis Research Center of China’s Ecosystem Research Network Key Laboratory of Ecosystem Network Observation and ModelingInstitute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China International institute for Earth System science Nanjing UniversityNanjing 210023China Tianjin Key Laboratory of Water Resources and Environment Tianjin Normal UniversityTianjin 300387China institute of Environment and Ecology Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control Collaborative Innovation Center of Atmospheric Environment and Equipment TechnologySchool of Environmental Science and EngineeringNanjing University of Information Science&TechnologyNanjing 210044China

As one of the world's largest emitters of greenhouse gases,China has set itself the ambitious goal of achieving carbon peaking and carbon ***,it is crucial to quantify the magnitude and trend of sources and sinks of atmospheric carbon dioxide(CO_(2)),and to monitor China's progress toward these *** state-of-the-art datasets and models,this study comprehensively estimated the anthropogenic CO_(2)emissions from energy,industrial processes and product use,and waste along with natural sources and sinks of CO_(2)for all of China during *** recognize the differences among various methods of estimating greenhouse emissions,the estimates are compared with China's National Greenhouse Gas Inventories(NGHGIs)for 1994,2005,2010,2012,and *** CO_(2)emissions in China have increased by 7.39 times from 1980 to 12.77 Gt CO_(2)a^(-1)in *** benefiting from ecological projects(e.g.,Three Norths Shelter Forest System Project),the land carbon sink in China has reached 1.65 Gt CO_(2)a^(-1)averaged through 2010-2021,which is almost 15.81 times that of the carbon sink in the *** average,China's terrestrial ecosystems offset 14.69%±2.49%of anthropogenic CO_(2)emissions through *** provincial-level administrative regions of China,Xizang and Qinghai,have achieved carbon neutrality according to our estimates,but nearly half of the administrative regions of China have terrestrial carbon sink offsets of less than 10%of anthropogenic CO_(2)*** study indicated a high level of consistency between NGHGIs and various datasets used for estimating fossil CO_(2)emissions,but found notable differences for land carbon *** estimates of the terrestrial carbon sinks of NGHGIs urgently need to be verified with process-based models which integrate the comprehensive carbon cycle processes.

关键词： CO_(2)emission Land carbon sink China Carbon neutrality Carbon budget

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禽类隐孢子虫与其他病原感染相关性研究进展

中国动物传染病学报 2024年第4期32卷 211-218页

作者：李易魏光伟李娟邱茜茜廖申权戚南山吕敏娜林栩慧蔡海明胡俊菁张健騑张浩吉孙铭飞佛山科学技术学院佛山528225 岭南现代农业科学与技术广东省实验室肇庆分中心/茂名分中心广东省畜禽疫病防治研究重点实验室/农业农村部兽用药物与诊断技术广东科学观测实验站农业农村部禽流感等家禽重大疾病防控重点实验室广州510640

隐孢子虫作为一种重要的胞内寄生原虫,感染谱广泛,主要侵犯呼吸道、胃肠道和法氏囊,禽类感染后主要表现为精神萎靡、腹泻、免疫低下和呼吸困难等临床症状。多重病原协同感染是近些年禽病的主要流行形式,禽类隐孢子虫作为一种重要的机会... 详细信息

隐孢子虫作为一种重要的胞内寄生原虫,感染谱广泛,主要侵犯呼吸道、胃肠道和法氏囊,禽类感染后主要表现为精神萎靡、腹泻、免疫低下和呼吸困难等临床症状。多重病原协同感染是近些年禽病的主要流行形式,禽类隐孢子虫作为一种重要的机会性致病病原,其感染与宿主的免疫功能直接相关,当器官/组织伴有其他病原同时感染时,可使疾病风险和防控难度加剧。目前,禽类隐孢子虫流行病学数据相对较少,与其他病原感染相关性研究尚不明朗。本文通过对国内外研究数据的梳理、归纳,对禽类隐孢子虫流行及其与呼吸道、消化道和法氏囊感染的其他病原的相关性进行综述,为进一步研究其间的协同致病机制奠定基础。

关键词：禽类隐孢子虫感染相关性禽胃肠道病原禽呼吸道病原禽法氏囊病原

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青海省莜麦资源特征及利用途经

青海师范大学学报(自然科学版) 1987年第4期 56-62页

作者：吴学明袁甲正蒋兴元青海师院大学生物系青海省农科院

本文从青海省莜麦的地理分布,种植利用、主要品种、营养成分等方面研究了我省莜麦的资源特征,提出了开发我省莜麦资源的途经。

关键词：莜麦地理分布苏联加拿大裸燕麦麦类作物苏维埃社会主义共和国联盟北美洲早熟品种燕麦品种青海省资源特征

基于大数据的中老年男性前列腺疾病科技服务云平台的构建

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中国当代医药 2022年第3期29卷 131-134页

作者：张旭辉张彬韩帅红郁磊荆强刘一凡靳祯王东文山西医科大学第一医院泌尿外科山西太原030001 山西大学复杂系统研究所山西太原030006 中国医学科学院北京协和医学院肿瘤医院深圳医院泌尿外科广东深圳518116

以中老年男性前列腺疾病为代表的慢性病的患者,多合并基础性疾病且免疫力较差,在我国人口老龄化趋势的当下,不仅具有较高的健康教育需求,而且存在较弱的科技服务接受能力、较低的病史信息传递效率和较差的用户依从性等特点,是中老年慢... 详细信息

以中老年男性前列腺疾病为代表的慢性病的患者,多合并基础性疾病且免疫力较差,在我国人口老龄化趋势的当下,不仅具有较高的健康教育需求,而且存在较弱的科技服务接受能力、较低的病史信息传递效率和较差的用户依从性等特点,是中老年慢性病在健康管理方面的代表性人群。为满足该群体在健康咨询、就医问诊、治疗随访中的科技服务需求,本研究将采集的中老年男性前列腺疾病大数据进行整理、加工后,通过K-Means聚类分析、深度神经网络等算法,绘制出相应的疾病知识图谱,并进行智能分析,从而实现了疾病预防及随诊过程中的人工智能辅助平台搭建。本平台的构建,为进一步探讨中老年男性前列腺疾病科技云服务的设计策略奠定了平台基础,也是中老年慢性病在健康管理上的一次有益尝试。

关键词：中老年男性前列腺疾病大数据人工智能云服务

Adaptive Generalized Synchronization for Chaotic Systems With Unknown Parameters based on Observer

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Adaptive Generalized Synchronization for Chaotic Systems Wit...

两类光照条件年份下遮膜模拟晴天霾情景对水稻生长与产量的影响研究

2010 Chinese Control and Decision Conference

作者： Yubo Duan~1,Luanjie Zhou~1,Ruiyun Wang~1,Keyong Shao~1,Tao Chang~2,Yishu Zhang~1 1.School of Electric and Information Engineering,Daqing Petroleum institute,Daqing,163318 2.Changqing’ Sulige Eastern Gas Ministry of Development Project,Sulige,017300,China

An adaptive synchronization control method based on state observer is proposed as for generalized synchronization for chaotic systems with unknown *** method uses a nonlinear state observer with a controller to realize generalized synchronization and estimate the unknown *** GS velocity can be improved according to configuration of poles,without a limit of stability of linear part of the chaotic *** unified system is taken for verification,and the results of the simulation indicated the efficiency of this method.

关键词： Chaotic System Generalized Synchronization State Observer Adaptive

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气象与环境科学 2022年第6期45卷 34-42页

作者：汪天颖张弥张富存越昆黄笑颖刘寿东肖薇王伟李旭辉湖南省气象科学研究所长沙410118 南京信息工程大学气象灾害预报预警与评估协同创新中心南京210044 气象防灾减灾湖南省重点实验室长沙410118 School of Forestry and Environmental Studies Yale UniversityNew Haven 06511USA

近年来,由于我国灰霾天气增多,灰霾天气对水稻产量的影响已成为粮食安全须关注的热点问题。通过2014年、2015年水稻透明塑料薄膜遮阴大田对比试验,模拟评估了晴天霾情景下太阳总辐射量减少、散射辐射比例增加的太阳光照环境对总光照条... 详细信息

近年来,由于我国灰霾天气增多,灰霾天气对水稻产量的影响已成为粮食安全须关注的热点问题。通过2014年、2015年水稻透明塑料薄膜遮阴大田对比试验,模拟评估了晴天霾情景下太阳总辐射量减少、散射辐射比例增加的太阳光照环境对总光照条件偏好(2015年)、总光照条件偏差(2014年)这两类典型年份水稻生长及其产量的影响,初步揭示出两类光照条件年份在霾情景对水稻生长规律、特点及其产量结构因子、产量的影响程度与作用机制。对比试验表明,两类光照条件年份在受到霾天气(晴天遮阴处理)影响后:(1)与对照组(CK)相比,处理组(T)在光照多的年份减产(9.95%)和光照少的年份减产(6.81%)均达到显著水平,减产率前者高出后者46.11%;光照多的年份受霾影响后产量甚至低于未曾受到霾影响的光照少的年份的产量。造成减产的主要原因是每平方米成穗数极显著减少,其次为千粒重降低、空秕率增加。(2)T的株高、叶片面积比CK的略有增加,且株高在灌浆开始之后差异较大,上层叶面积在分蘖末期之后差异显著,下层叶面积在灌浆成熟期差异显著;上层叶面积的差距总是大于下层同期的差距。(3)T的成熟期干物质重量一般呈显著减少,光照少的年份减少更为严重;茎秆贮存的干物质比例减少,通过增加叶片要素的比重维持了穗部的分配系数;光照多的年份干物质积累从茎秆转移至叶片,光照少的年份干物质减少而比叶面积增加。(4)T的上层、下层的叶面积均有所增加;成熟期单株叶重在光照少的年份呈极显著减少,在光照多的年份却显著增加;叶分配系数在不加处理时以光照少的年份为高,遮阴处理后以光照多的年份为高;成熟期比叶面积有增加,光照多的年份增加少(1.25%),而光照少的年份增加多(22.97%)。

关键词：太阳总辐射散射辐射比例日照时数水稻生长产量构成因子霾天气影响透明塑料膜遮阴

福建省丘陵区乡村高效生态农业发展思路与技术对策研究

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环境与可持续发展 2018年第1期43卷 118-125页

作者：刘朋虎赵雅静王义祥罗旭辉邢世和翁伯琦福建农林大学福建福州350002 福建省农业科学院福建福州350003 福建省农业科学院农业生态研究所福建福州350013 福建省红壤山地农业生态过程重点实验室福建福州350013

结合福建红壤丘陵山区农业生产实际,提出发展富有区域特色高效生态农业总体思路与战略思考。阐明在新时期发展高效生态农业的重要性与必要性,分析红壤丘陵区发展高效生态农业的优势与短板,以红壤丘陵区高效生态农业发展模式与技术集成... 详细信息

结合福建红壤丘陵山区农业生产实际,提出发展富有区域特色高效生态农业总体思路与战略思考。阐明在新时期发展高效生态农业的重要性与必要性,分析红壤丘陵区发展高效生态农业的优势与短板,以红壤丘陵区高效生态农业发展模式与技术集成研究为切入点,提出发展高效生态农业的主要模式与技术对策,以期为红壤丘陵山区农业转型升级与增效提质提供借鉴与参考。

关键词：丘陵山区红壤山地高效生态农业发展模式技术对策

Efficacy and safety of LY01005 versus goserelin implant in Chinese patients with prostate cancer: A multicenter, randomized, open-label, phase III, non-inferiority trial

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Chinese Medical Journal 2023年第10期136卷 1207-1215页

作者： Chengyuan Gu zengjun Wang Tianxin Lin Zhiyu Liu Weiqing Han xuhui Zhang Chao Liang Hao Liu Yang Yu Zhenzhou Xu Shuang Liu Jingen Wang Linghua Jia Xin Yao Wenfeng Liao Cheng Fu Zhaohui Tan Guohua He Guoxi Zhu Rui Fan Wenzeng Yang Xin Chen Zhizhong Liu Liqiang Zhong Benkang Shi Degang Ding Shubo Chen Junli Wei Xudong Yao Ming Chen Zhanpeng Lu Qun Xie Zhiquan Hu Yinhuai Wang Hongqian Guo Tiwu Fan Zhaozhao Liang Peng Chen Wei Wang Tao Xu Chunsheng Li Jinchun Xing Hong Liao Dalin He Zhibin Wu Jiandi Yu Zhongwen Feng Mengxiang Yang Qifeng Dou Quan zeng Yuanwei Li Xin Gou Guangchen Zhou Xiaofeng Wang Rujian Zhu Zhonghua Zhang Bo Zhang Wanlong Tan Xueling Qu Hongliang Sun Tianyi Gan Dingwei Ye Department of Urology Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Department of Urology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Urology Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhouGuangdong 510120China Department of Urology The Second Affiliated Hospital of Dalian Medical UniversityDalianLiaoning 116023China Department of Urology The Affiliated Cancer Hospital of Xiangya School of MedicineCentral South UniversityHunan Cancer CenterChangshaHunan 410013China Department of Urology First Hospital of Shanxi Medical UniversityTaiyuanShanxi 030000China Department of Urology Jiangxi Provincial People’s HospitalSchool of MedicineNanchang UniversityNanchangJiangxi 330006China Department of Urology Tianjin Medical University Cancer Institute and HospitalTianjin 300060China Department of Urology Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital and InstituteShenyangLiaoning 110043China Department of Urology Inner Mongolia People’s HospitalHohhotInner Mongolia 010010China Department of Urinary Surgery Taizhou Hospital of Traditional Chinese MedicineTaizhouJiangsu 225300China Department of Urology Linfen People’s HospitalLinfenShanxi 041000China Department of Urology Zhengzhou Central Hospital Affiliated to Zhengzhou UniversityZhengzhouHenan 450006China Department of Urology The Affiliated Hospital of Hebei UniversityBaodingHebei 071000China Department of Urology Chifeng Municipal HospitalChifeng Clinical Medical College of Inner Mongolia Medical UniversityChifengInner Mongolia 024000China Department of Urology Baogang HospitalBaotouInner Mongolia 014010China Department of Oncology The Second People’s Hospital of YibinYibinSichuan 644000China Department of Urology Qilu Hospital of Shandong UniversityJinanShandong 250014China Department of Urology Henan Provincial People’s Hos

Background:LY01005(Goserelin acetate sustained-release microsphere injection)is a modified gonadotropin-releasing hormone(GnRH)agonist injected *** phase III trial study aimed to evaluated the efficacy and safety of LY01005 in Chinese patients with prostate ***:We conducted a randomized controlled,open-label,non-inferiority trial across 49 sites in *** study included 290 patients with prostate cancer who received either LY01005 or goserelin implants every 28 days for three *** primary efficacy endpoints were the percentage of patients with testosterone suppression≤50 ng/dL at day 29 and the cumulative probability of testosterone≤50 ng/dL from day 29 to ***-inferiority was prespecified at a margin of-10%.Secondary endpoints included significant castration(≤20 ng/dL),testosterone surge within 72 h following repeated dosing,and changes in luteinizing hormone,follicle-stimulating hormone,and prostate specific antigen ***:On day 29,in the LY01005 and goserelin implant groups,testosterone concentrations fell below medical-castration levels in 99.3%(142/143)and 100%(140/140)of patients,respectively,with a difference of-0.7%(95%confidence interval[CI],-3.9%to 2.0%)between the two *** cumulative probabilities of maintaining castration from days 29 to 85 were 99.3%and 97.8%,respectively,with a between-group difference of 1.5%(95%CI,-1.3%to 4.4%).Both results met the criterion for *** endpoints were similar between *** treatments were ***01005 was associated with fewer injection-site reactions than the goserelin implant(0%vs.1.4%[2/145]).Conclusion:LY01005 is as effective as goserelin implants in reducing testosterone to castration levels,with a similar safety *** registration:***,NCT04563936.

关键词： Prostatic neoplasms LY01005 Gonadotropin-releasing hormone agonist Efficacy Safety