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Inheritance of marsh spot disease resistance in cranberry common bean(Phaseolus vulgaris L.)

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The Crop Journal 2022年第2期10卷 456-467页

作者： Bosen Jia Robert L.Conner Nadeem Khan Anfu Hou xuhua xia Frank M.You Ottawa Research and Development Centre Agriculture and Agri-Food CanadaOttawaOntario K1A 0C6Canada Department of Biology University of OttawaOttawaOntario K1N 6N5Canada Morden Research and Development Centre Agriculture and Agri-Food CanadaMordenManitoba R6M 1Y5Canada

Common bean(Phaseolus vulgaris) is an annual legume crop that is grown worldwide for its edible dry seeds and tender pods. Marsh spot(MS) of the seeds is a physio-genic stress disease affecting seed quality in beans. Studies have suggested that this disease involves a nutritional disorder caused by manganese deficiency, but the inheritance of resistance to this disease has not been reported. A biparental genetic population composed of 138 recombinant inbred lines(RILs) was developed from a cross between an MS resistant cultivar ‘Cran09’ and an MS susceptible cultivar ‘Messina’. The 138 RILs and their two parents were evaluated for MS resistance during five consecutive years from 2015 to 2019 in sandy and heavy clay soils in Morden, Manitoba, Canada. The MS incidence(MSI) and the MS resistance index(MSRI) representing disease severity were shown to be both highly correlated heritable traits that had high broad-sense heritability values(H;) of 86.5% and 83.2%, respectively. No significant differences for MSI and MSRI were observed between the two soil types in all five-(MSI) or four-year(MSRI) data collection, but significant correlations among years were observed despite MS resistance was moderately affected by year. The MSIs and MSRIs displayed a right-skewed distribution, indicating a mixed genetic model involving a few major genes and polygenes. Using the joint segregation analysis method, the same four major genes with additive-epistasis effects showed the best fit for both traits, explaining 84.4% and85.3% of the phenotypic variance for MSI and MSRI, respectively. For both traits, the M1, M2, M3 and m4 acted as the favorable(resistant) alleles for the four genes where M and m represent two alleles of each gene. However, due to epistatic effects, only the individuals of the M1 M2 M3 M4 haplotype appeared to be highly resistant, whereas those of the m1 m2 m3 M4 haplotype were the most susceptible. The m4 allele significantly suppressed the additive effects of M1 M

关键词： Common bean Cranberry bean Marsh spot Resistance Recombinant inbred line(RIL) Joint segregation analysis Major gene Polygene

Correlations between recombination rate and intron distributions along chromosomes of ***

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Progress in Natural Science:Materials International 2009年第4期19卷 517-522页

作者： Hong Li a,b, Guoqing Liu a, xuhua xia b a School of Physical Science and Technology, Inner Mongolia University, Hohhot 010021, China b Department of Biology, University of Ottawa, Ottawa, Ont., Canada K1N 6N5 School of Physical Science and Technology Inner Mongolia University Hohhot 010021 China Department of Biology University of Ottawa Ottawa Ont. Canada K1N 6N5

Generally speaking, the intron size positively correlates with recombination rate in Caenorhabditis elegans genome. Here, we analyze the correlations between recombination rate and some measures of different intron lengths so as to know whether the recombination influences the introns of different lengths in the same way. Results show that the correlation between the recombination rate and the percentage of short introns (<100 bp) is negative, but the correlation between the recombination rate and the percentage of introns that are larger than 500 bp is positive. Average intron length correlates positively with the recombination rate for introns whose length is in the range of 100-1000 bp. We speculate that the recombination mainly exerts impact on introns whose length ranges from 100-1000 bp. We also show that the average intron number per gene correlates negatively with the recombination rate.

关键词： Recombination rate Percentage of introns Intron length Intron number per gene C. elegans

Marsh Spot Disease and Its Causal Factor, Manganese Deficiency in Plants: A Historical and Prospective Review

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Agricultural Sciences 2021年第9期12卷 928-948页

作者： Bosen Jia Penner Waldo Robert Lorne Conner Ismael Moumen Nadeem Khan xuhua xia Anfu Hou Frank Mingan You Ottawa Research and Development Centre Agriculture and Agri-Food Canada Ottawa Canada Department of Biology University of Ottawa Ottawa Canada Morden Research and Development Centre Agriculture and Agri-Food Canada Morden Canada

This review provides an examination of the marsh spot disease in beans and the roles played by its causal factor, manganese (Mn) deficiency. The discovery of the marsh spot disease, its relation with Mn deficiency, and how it can be treated are discussed. Mn serves as a cofactor and a catalyst in various metabolic processes in different cell compartments, such as the oxygen-evolving complex of photosystem II (PSII) or reactive oxygen species scavenging. Some major quantitative trait loci (QTL) and putative candidate genes associated with Mn content in plants, especially in plant seeds, have been identified. Marsh spot disease in cranberry common bean is controlled by several major genes with significant additive and epistatic effects. They provide valuable clues for QTL candidate gene prediction and an improved understanding of the genetic mechanisms responsible for marsh spot resistance in plants.

关键词： Marsh Spot Manganese Deficiency Quantitative Trait Locus (QTL) Mn Transport Cranberry Common Bean

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德兴铜矿废水污染对乐安河上游藻类的影响

南昌大学学报(理科版) 1985年第4期 7-20页

作者：夏旭华江西大学生物系

乐安河是赣东北主要河流之一,源于赣皖边界的怀玉山西麓,流经务源、德兴、乐平、万年、波阳等县,全长279公里,流域面积达8367平方公里。德兴铜矿位于乐安河上游南岸,北纬29°41′,东经117°44′,全矿区总面积为100平方公

关键词：藻类孢子植物乐安河废水污染德兴铜矿

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新生儿侵袭性真菌感染诊治进展

儿科药学杂志 2021年第5期27卷 56-59页

作者：夏旭花(综述) 华子瑜(审校) 重庆医科大学附属儿童医院儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地儿科学重庆市重点实验室国家住院医师规范化培训示范基地重庆400014

近年来,随着新生儿重症监护病房(neonatal intensive care unit,NICU)中高危患儿的增多、广谱抗生素的广泛使用、各种侵入性操作增加及高级生命支持系统的应用,新生儿侵袭性真菌感染(invasive fungal infection,IFI)已成为NICU中早产儿... 详细信息

近年来,随着新生儿重症监护病房(neonatal intensive care unit,NICU)中高危患儿的增多、广谱抗生素的广泛使用、各种侵入性操作增加及高级生命支持系统的应用,新生儿侵袭性真菌感染(invasive fungal infection,IFI)已成为NICU中早产儿晚发型感染的主要病因[1]。发病率高、病死率高,但临床表现缺乏特异性,给临床医务工作者带来了一定的挑战。现分别从流行病学、临床特点、诊断和治疗等方面对新生儿IFI的研究进展进行综述,以期提高临床医务工作者对新生儿IFI的认识。

关键词：侵袭性真菌感染高级生命支持新生儿重症监护病房晚发型侵入性操作 NICU 流行病学广谱抗生素

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用于组织学光镜检查的树脂包埋法

南昌大学学报(理科版) 1985年第1期 64-67页

作者：夏旭华曾小鲁江西大学生物系

用树脂Resin包埋组织块进行薄切片已成为组织学和病理学中的一项常规技术。过去树脂包埋只是用做超薄切片和电镜观察,但目前国外此项技术还专门用做薄切片用光镜观察。虽然不能说树脂包埋可以完全取代石蜡包埋法,但人们逐渐认识到树脂... 详细信息

用树脂Resin包埋组织块进行薄切片已成为组织学和病理学中的一项常规技术。过去树脂包埋只是用做超薄切片和电镜观察,但目前国外此项技术还专门用做薄切片用光镜观察。虽然不能说树脂包埋可以完全取代石蜡包埋法,但人们逐渐认识到树脂包埋法与石蜡包埋法比较有不少优点。它能切得较薄、产生的人工假象较少和微细结构更加清晰。另外如确有必要使用电镜观察时,树脂包埋的标本还能进一步成功地制成超薄切片。Reichert-Jung厂发表的通报介绍了这一方法,为了使能在国内推广使用,我们根据该资料和参阅有关资料,将此方法整理介绍如下。

关键词：包埋法组织块光镜观察环氧树脂万能胶环氧化物包埋剂

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南翼山浅层油藏Ⅲ+Ⅳ油组压裂技术优化

油气井测试 2024年第2期33卷 51-57页

作者：吴明江杨明敏谢泽洪王传峰夏旭华张洲年中国石油集团西部钻探工程有限公司吐哈井下作业公司新疆鄯善838200

青海油田南翼山浅层油藏藻灰岩储层Ⅲ+Ⅳ油组剩余油挖潜程度不高,压裂液对储层伤害大导致压后效果差,通过岩石力学分析、暂堵转向机理研究、压裂液体系优化选择,压裂施工参数优化,形成“机械剖面细分层+层间暂堵转向+缝内暂堵转向”组... 详细信息

青海油田南翼山浅层油藏藻灰岩储层Ⅲ+Ⅳ油组剩余油挖潜程度不高,压裂液对储层伤害大导致压后效果差,通过岩石力学分析、暂堵转向机理研究、压裂液体系优化选择,压裂施工参数优化,形成“机械剖面细分层+层间暂堵转向+缝内暂堵转向”组合压裂工艺技术,有效解决老井重复压裂挖潜剩余油程度不高难题。现场试验8井次,措施成功率100%,压后初期平均单井日产液13.3 m3/d,日产油3.4 t/d,效果比常规机械分层压裂提高55%,达到预期增油效果,应用效果显著。该技术对同类型储层压裂改造有很好的借鉴意义。

关键词：藻灰岩非均质薄互层机械剖面分层暂堵转向压裂液体系岩石力学剩余油挖潜

Impacts of competition and nitrogen addition on plant stoichiometry and non-structural carbohydrates in two larch species

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Journal of Forestry Research 2021年第5期32卷 2087-2098页

作者： xiaoyi Wu xuhua Du Shiyan Fang Jieyu Kang Zhichao xia Qingxue Guo College of Life and Environmental Sciences Hangzhou Normal UniversityHangzhou 310036People’s Republic of China Key Laboratory of State Forestry Administration on Bamboo Resources and Utilization China National Bamboo Research CenterHangzhou 310012People’s Republic of China

Previous research has shown that competition between plants can have differential effects on leaf stoichiometry and non-structural carbohydrate(NSC)in different ***,little attention has been given to understanding these effects on non-photosynthetic organs,particularly of deciduous tree *** we assess the impact of competition on below and aboveground biomass,stoichiometry,nutrient composition and NSC in pure and mixed forests of two Larch species,Larix kaempferi and *** under nitrogen(N)*** enrichment did not result in stronger intraspecific competition for both species and *** benefited from the presence of *** under different N *** kept relatively stable C/N compared to roots and branches in response to competition,while N addition imposed stronger impacts on N/P of different organs rather than *** contrast to stable C concentrations,starch and soluble sugar concentrations were more easily impacted by competition and the addition of *** forced *** and *** to allocate more carbon into storage by increasing their starch concentration and starch/soluble sugar of stems under ***,no significant differences in stoichiometry and NSC concentration between intra-and interspecific competition were *** and nutrient pools of *** stems,branches and coarse roots consistently declined due to competition regardless of N *** and fine roots of *** accumulated more N when in competition with *** than with a conspecific neighbor under N *** results show that NSC was more sensitive to competition relative to stoichiometric traits(N and P)of non-photosynthetic organs.

关键词： Intraspecific and interspecific competition Non-photosynthetic organs Resource competition Remobilization and storage Mixed forest plantations

肯基亚克油田盐上斜坡带低温低压砂岩油藏压裂技术研究

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石油地质与工程 2024年第4期38卷 77-82页

作者：王勇邓有根夏旭华葸尚勇周海元董世才中国石油西部钻探工程有限公司吐哈井下作业公司新疆鄯善838200

哈萨克斯坦肯基亚克油田盐上斜坡带二叠系砂岩油藏主力储层埋深900~1350 m,原始地层压力系数1.0左右,储层温度25~35℃。由于油藏为高角度单斜油藏,邻井同层连通性差,且油藏南北跨距小,不具备注水开发井网部署条件,区内油井投产后一直采... 详细信息

哈萨克斯坦肯基亚克油田盐上斜坡带二叠系砂岩油藏主力储层埋深900~1350 m,原始地层压力系数1.0左右,储层温度25~35℃。由于油藏为高角度单斜油藏,邻井同层连通性差,且油藏南北跨距小,不具备注水开发井网部署条件,区内油井投产后一直采用衰竭式开采,目前地层压力系数已降至0.61左右,单井产能已低至2~5 t/d。为探索加砂压裂技术在该类油藏的适应性,针对低温、低压油藏压裂压裂液破胶难度大、压后返排困难等技术难题,开展了加砂压裂措施增产潜力分析、低温破胶压裂液体系评价优选、低压油藏压后排液工艺优化等室内研究,并将研究成果于Kxxx9井开展了首口井应用,压裂增产倍数达到8倍以上,措施效果显著,为低温低压油藏开发提供了新的技术思路。

关键词：低温低压单斜油藏衰竭式开采破胶加砂压裂