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Deletion of a Dna K protein gene causes seedling green-revertible albino by retarding chloroplast development in rice

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Science Bulletin 2015年第23期60卷 2054-2058页

作者： Weitao Li Junjie Yin Bangquan Ye Chunfang Peng Qinshu cheng Jing Wang Can Yuan Heng Yin Zhixiong chen Min He Yuping Wang Weilan chen Jichun Wang Bingtian Ma Peng Qin Shigui Li xuewei chen Rice Research Institute Key Laboratory of Major Crop DiseasesState Key Laboratory of Hybrid Rice and Collaborative Innovation Center for Hybrid Rice in Yangtze River BasinSichuan Agricultural University

Green-revertible albino mutants are important sources for studying chloroplast structure, chloroplast development, chlorophyll biosynthesis, and plant photosynthesis. In the present study, we characterized a greenrevertible albino mutant gra(k), which was obtained from the tissue-cultured rice Kitaake. The mutant gra(k) exhibited albino on its first three leaves. The leaf color started to turn green at the four-leaf stage. The chlorophyll contents were deeply reduced at the seedling stage, and the chloroplast development was delayed in gra(k). The greenrevertible albino(gra) phenotype of the mutant gra(k) was temperature dependent. The main agronomic traits,including plant height, tilling number per plant, seed setting rate, and thousand-grain weight, slightly decreased in gra(k) comparing to those in the wild-type *** analysis showed that the gra phenotype was controlled by a single recessive nucleic gene. By using 5,168 recessive F2individuals derived from the cross of gra(k) 9 Jodan, the locus of the gene Gra(k) was delimited in a DNA region of 200 kb between the makers B-31 and P11 on chromosome 5. Sequencing analysis indicated thatthe three functionally annotated genes, LOC_Os05g23700,LOC_Os05g23720, and LOC_Os05g23740, were all deleted in the 200 kb region in the mutant gra(k). Transgenic test revealed that the gra(k) plants over-expressing LOC_Os05g23740CDS were restored to normal green as the wild-type Kitaake. Our results proved that the deletion of the Dna K protein gene LOC_Os05g23740(encoding the chaperon protein Os Hsp70CP1) led to the gra phenotype in the mutant gra(k).

关键词：叶绿体发育叶片白化基因缺失水稻幼苗蛋白叶绿素含量基因植入突变体

Characterization and Fine Mapping of a Novel Vegetative Senescence Lethal Mutant Locus in Rice

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Journal of Genetics and Genomics 2015年第9期42卷 511-514页

作者： Junjie Yin Xiaobo Zhu Can Yuan Jing Wang Weitao Li Yuping Wang Min He Qinshu cheng Bangquan Ye Weilan chen Qianyan Linghu Jichun Wang Bingtian Ma Peng Qin Shigui Li xuewei chen Rice Research Institute Key Laboratory of Major Crop Diseases and State Key Laboratory of Hybrid Rice Sichuan Agricultural University

Mutants showing spontaneous cell death in the absence of pathogen attack are called lesion mimic mutants （lmm）（Lorrain et al., 2003）. These mutants usually exhibit typical hypersensitive responses （HRs） within or around the lesion spots, which are frequently observed in plants challenged with avirulent pathogens （Lorrain et al., 2003）. A number of these mutants have been characterized in rice （Zeng et al., 2004）, Arabidopsis （Guo et al., 2013）, maize （Wang et al., 2013） and barley （Persson et al., 2008）. Most lmm show enhanced resistance to various pathogens （Huang et al., 2011）, because HR is usually accompanied with enhanced defense responses, such as reactive oxygen species （ROS） activation （Qiao et al., 2010） and increased expression of pathogenesis-related genes （Lorrain et al., 2003）. Additionally, most lmm exhibit defects in growth and development due to the disordered physiolog- ical and molecular processes caused by the lesion spots. Thus, lmm are powerful tools for the study of the molecular mech- anisms of cell death, plant development and disease resistance （Lorrain et al., 2003; Babu et al., 2011）.

关键词： Characterization and Fine Mapping of a Novel Vegetative Senescence Lethal Mutant Locus in Rice gene

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大坝泄流水体溶解气体饱和度预测方法初探

水力发电学报 2010年第6期29卷 138-143页

作者：程香菊陈雪巍中山大学工学院海洋工程研究中心广州510275

大型水电工程对水生态环境的影响一直是国内外广泛关注的问题。当水库闸坝泄流时,可能会使下游水体溶解气体浓度超饱和,导致鱼类等水生动物患气泡病,威胁它们的生存和繁殖。如何准确而快速地计算或预测高坝泄洪水体溶解气体的浓度迫在... 详细信息

大型水电工程对水生态环境的影响一直是国内外广泛关注的问题。当水库闸坝泄流时,可能会使下游水体溶解气体浓度超饱和,导致鱼类等水生动物患气泡病,威胁它们的生存和繁殖。如何准确而快速地计算或预测高坝泄洪水体溶解气体的浓度迫在眉睫。本文根据泄水建筑物实测数据,计算出泄流水体气体传质速率,并发现在水力条件一定时,闸坝泄水建筑物气体传质速率不变的特性,在此基础上,结合有效饱和浓度、有效水深以及上游溶解气体饱和度等参数,建立了对闸坝下游水体溶解气体饱和度进行预测的方法。预测结果表明,该方法具有计算简便且应用广泛的特点。

关键词：环境水力学预测方法气体传质速率有效饱和浓度溶解气体饱和度有效水深

Molecular mechanism of transcription factors regulating rice blast resistance

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Molecular mechanism of transcription factors regulating rice...

中国共产党意识形态建设的三重逻辑:历史、理论与实践

中国植物病理学会2019年学术年会

作者： Li Weitao Zhu Ziwei chen xuewei State Key Laboratory of Discovery and Utilization of Crop Genetic Resources in Southwest China (In preparation) State Key Laboratory of Hybrid RiceKey Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River BasinState Key Laboratory of Hybrid RiceHunan Hybrid Rice Research CenterRice Research InstituteSichuan Agricultural University at Wenjiang

Rice blast is the most devastating disease of *** effectively control this disease,it is necessary to elucidate molecular functions of resistance-related *** have compared the transcriptomic profile between the durably resistant rice variety Digu and the susceptible rice variety Lijiangxintuanheigu in response to infection by Magnaporthe oryzae,prior to full development of the *** identified 37 transcription factors(TFs) which regulate the blast response in the durably resistant rice Digu before the full maturation of the appressorium of ***,we found that many biological processes were specifically activated in Digu shortly after infection,such as extracellular recognition,biosynthesis of antioxidants,terpenes and *** results above indicated that contact and perception were present before the full maturation of the appressorium in the interaction of *** also identified a natural allele of bsr-dl that confers broad-spectrum resistance to blast ***,this allele causes a single nucleotide change in the promoter of the Bsr-dl gene,resulting in reduced expression of this gene due to the enhanced binding by its repressive TF MYBS1 and,consequently,an inhibition of H O degradation and enhanced disease ***,our studies advance and deepen understanding of blast disease resistance.

关键词： Transcription factor Resistance Broad-spectrum Magnaporthe oryzae Rice

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中共福建省委党校（福建行政学院）学报 2024年第3期 63-69页

作者：陈潜陈步云王雪薇福建农林大学马克思主义学院邯郸学院马克思主义学院

意识形态工作是中国共产党的一项极为重要的工作。其建设过程,就是我们党对马克思主义主动选择、不断发展并与时俱进的过程,蕴含着深刻的历史、理论和实践逻辑。其中,历史逻辑是根基,理论逻辑在历史进程中形成,又在实践逻辑中得到发展,... 详细信息

意识形态工作是中国共产党的一项极为重要的工作。其建设过程,就是我们党对马克思主义主动选择、不断发展并与时俱进的过程,蕴含着深刻的历史、理论和实践逻辑。其中,历史逻辑是根基,理论逻辑在历史进程中形成,又在实践逻辑中得到发展,我们应在梳理党百年来意识形态建设的历史脉络中分析和把握其内在的理论遵循和实践要求;实践逻辑包含在具体的历史逻辑中,是对历史逻辑的延伸和发展,又随着时代条件的变化而提出新要求,成为理论创新的源泉和动力,推动理论逻辑的不断发展,彰显着我们党对马克思主义科学理论的真理性认识;历史逻辑和理论逻辑统一于实践逻辑,要求我们在回应实践中积极推进党的意识形态建设。

关键词：中国共产党意识形态建设历史逻辑理论逻辑实践逻辑

同方期刊数据库

基于改进零脉冲插入法的永磁同步电机相电流重构策略

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电机与控制学报 2024年第6期28卷 25-35页

作者：陈思宇向学位李辉周豪蒋鹏重庆大学输配电装备及系统安全与新技术国家重点实验室重庆400044

直流母线单电流采样永磁同步电机系统存在相电流重构盲区,导致电流采样失真,提出一种改进零脉冲插入法,实现相电流重构。首先,分析传统固定零脉冲插入法的缺点,由于各相插入零脉冲作用时间相同,未能发挥“双开关PWM”模式抑制电流纹波... 详细信息

直流母线单电流采样永磁同步电机系统存在相电流重构盲区,导致电流采样失真,提出一种改进零脉冲插入法,实现相电流重构。首先,分析传统固定零脉冲插入法的缺点,由于各相插入零脉冲作用时间相同,未能发挥“双开关PWM”模式抑制电流纹波的优势;其次,通过各相PWM占空比调整零脉冲时间,延长电流采样窗口,提出改进零脉冲插入法重构策略,消除重构盲区,并针对该方法重构盲区出现其他扇区有效电压矢量的情况,分析其空间电压矢量合成关系,证明参考电压矢量合成的准确性;最后,基于戴维南电路法,提出不同电压矢量作用时的电路简化模型,对比两种方法的电流纹波幅值,并搭建永磁同步电机实验平台进行实验。结果表明,改进零脉冲插入法的电流重构误差低于3%,相比传统方法,电流纹波抑制效果达到50%,相电流畸变率从12.72%下降到6.87%。

关键词：单电流传感直流母线采样相电流重构重构盲区永磁同步电机零脉冲插入法

维普期刊数据库博看期刊

文化消费对公众幸福感的影响——获得感的中介作用与主观阶层感知的调节作用

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文化产业研究 2023年第1期32卷 103-118页

作者：陈雪薇张铮华东政法大学传播学院清华大学新闻与传播学院清华大学文化创意发展研究院

文化消费是衡量民生福祉的重要指标。本文使用中国综合社会调查数据,试图从获得感的理论视角出发,构建一个有调节的中介模型,旨在探究公众文化消费与获得感、幸福感的关系效应及其边界条件。在以频率与多样性作为文化消费的测量指标的... 详细信息

文化消费是衡量民生福祉的重要指标。本文使用中国综合社会调查数据,试图从获得感的理论视角出发,构建一个有调节的中介模型,旨在探究公众文化消费与获得感、幸福感的关系效应及其边界条件。在以频率与多样性作为文化消费的测量指标的基础上进行研究,结果表明,文化消费与幸福感之间存在显著的正相关关系;文化消费与获得感具有显著的正相关关系;获得感部分中介了文化消费与幸福感之间的正相关关系;主观阶层认同调节了文化消费与幸福感之间的关系以及获得感的中介效应。研究结果拓展了文化消费与民生福祉的关系研究,对丰富人民群众精神文化生活具有启发意义。

关键词：文化消费获得感幸福感主观阶层认同

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高分六号卫星的交叉辐射定标研究

计算机应用与软件 2022年第10期39卷 54-58,146页

作者：纪睿谢勇余涛陈雪薇邵雯南京信息工程大学地理科学学院江苏南京210044 遥感卫星应用国家工程实验室南京研究中心江苏南京210044 中国科学院遥感与数字研究所北京100101 南京信息工程大学电子与信息学院江苏南京210044

为了能够检测高分六号卫星的辐射性能并准确地计算出高分六号卫星的辐射定标系数,利用高分六号卫星的成像特性,主要针对辐射定标区域和卫星自身成像特性对交叉辐射定标方法进行了优化,从而计算得到了高分六号卫星的增益和偏移。结果表... 详细信息

为了能够检测高分六号卫星的辐射性能并准确地计算出高分六号卫星的辐射定标系数,利用高分六号卫星的成像特性,主要针对辐射定标区域和卫星自身成像特性对交叉辐射定标方法进行了优化,从而计算得到了高分六号卫星的增益和偏移。结果表明多场地交叉辐射定标法各场地的不确定度分别为1.94%、1.91%、1.92%、2.77%都在4%以内,远低于单场地交叉辐射定标法得到的不确定度,提高了定标的精度。

关键词：高分六号卫星几何校正交叉定标法不确定度多场地

Identification and characterization of rice blast resistance gene Pid4 by a combination of transcriptomic profiling and genome analysis

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Journal of Genetics and Genomics 2018年第12期45卷 663-672页

作者： Zhixiong chen Wen Zhao Xiaobo Zhu chengdong Zou Junjie Yin Mawsheng Chern Xiaogang Zhou Heng Ying Xin Jiang Yongzhen Li Haicheng Liao Mengping cheng Weitao Li Min He Jing Wang Jichun Wang Bingtian Ma Jirui Wang Shigui Li Lihuang Zhu xuewei chen State Key Laboratory of Crop Genetics of Disease Resistance and Disease Control State Key Laboratory of Hybrid RiceKey Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River BasinRice Research InstituteSichuan Agricultural UniversitySichuan 611130 China Department of Plant Pathology University of CaliforniaDavisCA 95616USA Triticeae Research Institute.Sichuan Agricultural University Sichuan 611130China State Key Laboratory of Plant Genomics National Center for Plant Gene ResearchInstitute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101China

Map-based cloning of plant disease resistance (R) genes is time-consuming. Here, we reported the isolation of blast R gene Pid4 using comparative transcriptomic profiling and genome-wide sequence analysis. Pid4 encodes a coiled-coil nucleotide-binding site leucine-rich repeat(CC-NBS-LRR) protein and is constitutively expressed at diverse developmental stages in the rice variety Digu. The Pid4 protein is localized in both the nucleus and cytoplasm. Introduction of Pid4 into susceptible rice cultivars confers race-specific resistance to leaf and neck blast. Amino acid sequence comparison and blast resistance spectrum tests showed that Pid4 is a novel R gene, different from the previously reported R genes located in the same gene cluster. A Pid4 Indel marker was developed to facilitate the identification of Pid4 in different rice varieties. We demonstrated that a plant R gene can be quickly isolated using transcriptomic profiling coupled with genome-wide sequence analysis.

关键词： Blast disease Genome-wide sequence analysis Resistance Rice Transcriptome