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NAs联合乙肝疫苗提高CHB经治患者HBsAg清除率的研究

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中国卫生标准管理 2017年第3期8卷 62-64页

作者：刘江福林琪王星郭如意苏智军余雪平郑怡娟张华堂福建医科大学附属泉州第一医院感染科福建泉州362000

目的分析重组乙肝疫苗治疗提高慢乙肝经治患者HBsAg清除率的有效性。方法选取108例慢乙肝经治患者,分为联合乙肝疫苗组54例,原有抗病毒方案联合接种60μg重组乙肝疫苗每月1次,对照组54例继续原有抗病毒方案。结果治疗结束后联合乙肝疫苗... 详细信息

目的分析重组乙肝疫苗治疗提高慢乙肝经治患者HBsAg清除率的有效性。方法选取108例慢乙肝经治患者,分为联合乙肝疫苗组54例,原有抗病毒方案联合接种60μg重组乙肝疫苗每月1次,对照组54例继续原有抗病毒方案。结果治疗结束后联合乙肝疫苗组HBsAg滴度下降幅度为44.46 IU/ml,联合乙肝疫苗组HBsAg水平下降优于对照组20.89 IU/ml,差异有统计学意义(P=0.03)。HBs Ab滴度上升弧度为26.04 m IU/ml,而对照组HBs Ab反而下降0.20 m IU/ml,差异有统计学意义(P=0.002)。联合乙肝疫苗组HBsAg阴转联合乙肝疫苗组有18例(33.33%),显著高于对照组有2例(3.70%)差异有统计学意义。(χ~2=15.709,P=0.000)。联合乙肝疫苗组HBsAg血清转化19例(35.19%)高于对照组1例(0.02%),差异有统计学意义(χ~2=19.882,P=0.000)。结论联合重组乙肝疫苗治疗慢乙肝经治患者可以有效提高HBsAg清除率及HBsAg血清转换率,价格低廉,安全可靠。

关键词：慢性乙型肝炎乙肝疫苗干扰素核苷类似药物

Mechanistic action of the acute toxicity of Bajitian(Morinda officinalis)in zebrafish embryos

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Journal of Traditional Chinese Medical Sciences 2020年第3期7卷 308-315页

作者： Lirong Tan Min Cheng Gigi Chi Ting Auyeung Oscar yu Suen Chan xueping Chen Infinitus(China)Company Ltd. Guangzhou529100China Vitargent(International)Biotechnology Limited. Hong Kong SAR999077China College of Veterinary Medicine and Life Science City University of Hong KongHong Kong SAR999077China

Objectives:To investigate acute toxicity of Bajitian(Morinda officinalis)in zebrafish ***:Zebrafish embryos at 48-h post fertilization(hpf)were exposed to Bajitian ethanol extract for72 *** causative action of a delay in yolk sac absorption by Bajitian was investigated by RT-PCR analysis of lipid metabolism-related microsomal triglyceride transfer protein(MTP),apolipoprotein CII(ApoC2)and lipogenesis-related liver x receptor(LXR)*** effect of Bajitian eliciting an inflammatory response was studied by exposing 72 hpf myeloperoxidase(MPO):GFP transgenic zebrafish embryos to Bajitian extract for 4 *** was done by TUNEL,caspase-3/7,and RT-PCR analysis of the apoptosis related pathway B-cell lymphoma 2 associated X protein(Bax),Nuclear factor kappa-lightchain-enhancer of activated B cells(NF-k B)genes,neutrophil development-related stem cell leukaemia(SCL)and transcription factor PU.1 genes,to reveal the causative action of Bajitian reducing ***:RT-PCR analysis found that Bajitian extract had no effect on the expression of MTP or ApoC2 genes,but upregulated LXR gene,which might explain the delay in yolk sac *** of the inflammatory response showed that compared with negative controls,Bajitian extract significantly(P<.05)reduced the number of neutrophils in MPO:GFP ***,caspase-3/7,and RT-PCR analysis of Bax and NF-k B genes found that Bajitian extract did not trigger the cell *** RT-PCR analysis found that Bajitian extract did not affect SCL expression,but did lead to down-regulation of *** inhibition of neutrophil development/differentiation may explain the decline in the total number of neutrophils following Bajitian treatment,which could be attributed to the anti-inflammatory effects found clinically for this ***:This study demonstrated that Bajitian caused a delay in yolk sac absorption and a decrease neutrophil in zebrafish embryos,which may be related to the inhibit

关键词： Bajitian Morinda officinalis Neutrophil Zebrafish PU.1 Liver x receptor(LXR) Anti-inflammation

Better prognostic determination of cT3 rectal cancer through measurement of distance to mesorectal fascia:A multicenter study

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Chinese Journal of Cancer Research 2021年第5期33卷 606-615页

作者： Xiaoyan Zhang Qiaoyuan Lu Xiangjie Guo Wuteng Cao Hongmei Zhang Tao yu Xiaoting Li Zhen Guan xueping Li Ruijia Sun Yingshi Sun Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of RadiologyPeking University Cancer Hospital&InstituteBeijing 100142China Department of Forensic Medicine Shanxi Medical UniversityTaiyuan 030001China Department of Radiology the Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510655China Department of Radiology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Medical Imaging Cancer Hospital of China Medical UniversityShenyang 110042China

Objective:To forward the magnetic resonance imaging(MRI)based distance between the deepest tumor invasion and mesorectal fascia(DMRF),and to explore its prognosis differentiation value in cT3 stage rectal cancer with comparison of cT3 ***:This was a retrospective,multicenter cohort study including cT3 rectal cancer patients undergoing neoadjuvant chemoradiotherapy followed by radical surgery from January 2013 to September *** and cT3 substage were evaluated from baseline *** cutoff of DMRF was determined by disease *** cox regression was used to test the prognostic values of baseline ***:A total of 804 patients were included,of which 226(28.1%)developed progression.A DMRF cutoff of7 mm was *** category,the clock position of the deepest position of tumor invasion(CDTI)and extramural venous invasion(EMVI)were independent predictors for disease progression,and hazard ratios(HRs)were 0.26[95%confidence interval(95%CI),0.13-0.56],1.88(95%CI,1.33-2.65)and 1.57(95%CI,1.13-2.18),***3 substage was not a predictor for disease ***:The measurement of DMRF value on baseline MRI can better distinguish cT3 rectal cancer prognosis rather than cT3 substage,and was recommended in clinical evaluation.

关键词： Rectal cancer T3 stage substage distance to mesorectal fascia magnetic resonance imaging

The symptom difference induced by Tobacco mosaic virus and Tomato mosaic virus in tobacco plants containing the N gene is determined by movement protein gene

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Science China(Life Sciences) 2004年第6期47卷 503-509页

作者： yu Cui1, HU Dongwei1, DONG Jiahong2, CUI Xiaofeng1, WU Junjie1, yu Jialin2 & ZHOU xueping1 1. Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China 2. National Key Laboratory on Agrobiotechnology, China Agricultural University, Beijing 100094, China Correspondence should be addressed to Zhou xueping 1. Institute of Biotechnology Zhejiang University 310029 Hangzhou China 2. National Key Laboratory on Agrobiotechnology China Agricultural University 100094 Beijing China

Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) are two closely related viruses in the genus Tobamovirus, but they induce obviously different sizes of necrotic lesions in tobacco plants containing the N gene. Comparison of the symptoms produced by TMV, ToMV and a chimaeric virus (T/OMP), in which the TMV movement protein (MP) gene was re- placed by the ToMV MP gene, showed T/OMP caused necrotic lesions that were similar in size to those of ToMV in tobacco plants containing the N gene. The coat protein and MP of the three viruses accumulated in planta with similar levels, and the replication level of TMV and T/OMP in protoplasts also had no difference. Comparison of the activities of defense-related enzymes (PAL, POD and PPO) induced by the three viruses also showed that the variability of enzyme activity induced by T/OMP was similar to that induced by TMV, but different from that induced by ToMV. The results indicate that the size difference of necrotic lesions induced by TMV and ToMV in to- bacco plants containing the N gene results from the functional difference of their MP genes.

关键词： Tobacco mosaic virus, Tomato mosaic virus, chimaeric virus, movement protein, necrotic lesions.

Structural Origin of the Red-Ribbon Style Iron Ores in the Xinyu Iron Deposit,Central Jiangxi Province

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Acta Geologica Sinica(English Edition) 2015年第2期89卷 691-692页

作者： CHEN Zhengle ZHANG Qing CHEN Bailin WANG xueping SHEN Tao WU Junjie Institute of Geomechanics Chinese Academy of Geological Sciences East China Institute of Technology Geological Investigation Team in Western Jiangxi Province

The Xinyu iron deposit, located in central Jiangxi Province, is one of the most important BIF-type deposits in China. It is hosted in the Late Proterozoic volcanic- sedimentary rocks, which are composed of sericite- chlorite pyhllite, magnetite-bearing chlorite phyllite or schist, magnetite quartzite, and schist （yu et al., 1989; Zeng et al., 2011）.

关键词： Structural Origin of the Red-Ribbon Style Iron Ores in the Xinyu Iron Deposit Central Jiangxi Province

Gli1 promotes epithelial-mesenchymal transition and metastasis of non-small cell lung carcinoma by regulating snail transcriptional activity and stability

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Acta Pharmaceutica Sinica B 2022年第10期12卷 3877-3890页

作者： xueping Lei Zhan Li Yihang Zhong Songpei Li Jiacong Chen yuanyu Ke Sha Lv Lijuan Huang Qianrong Pan Lixin Zhao Xiangyu Yang Zisheng Chen Qiudi Deng Xiyong yu Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target&Clinical Pharmacology the NMPA and State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436China Department of Respiratory and Critical Care Medicine the Sixth Affiliated Hospital of Guangzhou Medical UniversityQingyuan People’s HospitalQingyuan 511518China GMU-GIBH Joint School of Life Sciences Guangzhou Medical UniversityGuangzhou 511436China

Metastasis is crucial for the mortality of non-small cell lung carcinoma(NSCLC) *** epithelial-mesenchymal transition(EMT) plays a critical role in regulating tumor ***-associated oncogene 1(Gli1) is aberrantly active in a series of tumor tissues. However, the molecular regulatory relationships between Gli1 and NSCLC metastasis have not yet been identified. Herein,we reported Gli1 promoted NSCLC metastasis. High Gli1 expression was associated with poor survival of NSCLC patients. Ectopic expression of Gli1 in low metastatic A549 and NCI-H460 cells enhanced their migration, invasion abilities and facilitated EMT process, whereas knock-down of Gli1 in high metastatic NCI-H1299 and NCI-H1703 cells showed an opposite effect. Notably, Gli1 overexpression accelerated the lung and liver metastasis of NSCLC in the intravenously injected metastasis model. Further research showed that Gli1 positively regulated Snail expression by binding to its promoter and enhancing its protein stability, thereby facilitating the migration, invasion and EMT of NSCLC. In addition, administration of GANT-61, a Gli1 inhibitor, obviously suppressed the metastasis of NSCLC. Collectively, our study reveals that Gli1 is a critical regulator for NSCLC metastasis and suggests that targeting Gli1 is a prospective therapy strategy for metastatic NSCLC.

关键词： Non-small cell lung carcinoma Metastasis Epithelialemesenchymal transition Glioma-associated oncogene 1 Promote Snail Protein stability GANT-61

一例COVID-19核酸检测阳性个案的应急处置报告

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中国麻风皮肤病杂志 2022年第8期38卷 499-502页

作者：槐鹏程李珍马衍鹏韦宝建杨旭日张兆升王川于功奇王猛程艳如孙远航韩淑馨杜文莉孙乐乐温绪岽刘军孙勇虎于学萍郭来雨曹印发刘红许庆贺张福仁山东第一医科大学附属皮肤病医院(山东省皮肤病医院) 山东省皮肤病性病防治研究所济南250022

背景:2022年4月13日晚8点,笔者所在医院设置在院外的为社会提供COVID-19核酸检测“愿检尽检”样本中发现1管10∶1混检管为阳性,为迅速锁定感染者、控制新冠疫情传播风险,特开展本次应急调查和处置。方法:参照《新型冠状病毒肺炎防控方案... 详细信息

背景:2022年4月13日晚8点,笔者所在医院设置在院外的为社会提供COVID-19核酸检测“愿检尽检”样本中发现1管10∶1混检管为阳性,为迅速锁定感染者、控制新冠疫情传播风险,特开展本次应急调查和处置。方法:参照《新型冠状病毒肺炎防控方案(第八版)》、《山东省医疗机构发现新冠病毒感染者应急处置指引》及本院《新型冠状病毒肺炎应急处置预案》,进行信息报告、院区封锁、人员追踪、密接筛查、采样消杀、核酸检测、风险评估等应急处置工作。结果:发现COVID-19无症状感染者1例,50岁女性,系医院周边劳务市场的“零工”,病毒毒株基因测序为奥密克戎BA.2。判定密接接触者65例,均落实隔离管控措施。医院全员新冠核酸检测、环境新冠核酸采样检测结果均为阴性,排除院内新冠传播风险,4月14日上午8点,医院在封控12小时后正常开诊。随后7天,对全院职工一天两检,结果均为阴性,之后恢复每天一检。结论:结合文件要求和医院实际制定详细可执行的新冠应急处置预案并参照预案内容进行演练,可提高医院新冠阳性病例的应急处置效率,为控制新冠疫情院内传播风险,恢复医院正常诊疗秩序赢得宝贵时间。

关键词：新型冠状病毒无症状感染者应急处置

Plant and animal positive-sense single-stranded RNA viruses encode small proteins important for viral infection in their negative-sense strand

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Molecular Plant 2023年第11期16卷 1794-1810页

作者： Pan Gong Qingtang Shen Mingzhen Zhang Rui Qiao Jing Jiang Lili Su Siwen Zhao Shuai Fu yu Ma Linhao Ge Yaqin Wang Rosa Lozano-Durán Aiming Wang Fangfang Li xueping Zhou State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina School of Basic Medical Sciences Fujian Medical UniversityFuzhouChina State Key Laboratory of Rice Biology and Breeding Institute of BiotechnologyZhejiang UniversityHangzhouZhejiangChina University of the Chinese Academy of Sciences BeijingChina Department of Plant Biochemistry Center for Plant Molecular Biology(ZMBP)Eberhard Karls University72076 TübingenGermany London Research and Development Center Agriculture and Agri-Food CanadaLondonONCanada

Positive-sense single-stranded RNA(+ssRNA)viruses,the most abundant viruses of eukaryotes in nature,require the synthesis of negative-sense RNA(-RNA)using their genomic(positive-sense)RNA(+RNA)as a template for *** on current evidence,viral proteins are translated via viral+RNAs,whereas-RNA is considered to be a viral replication intermediate without coding ***,we report that plant and animal+ssRNA viruses contain small open reading frames(ORFs)in their-RNA(reverse ORFs[rORFs]).Using turnip mosaic virus(TuMV)as a model for plant+ssRNA viruses,we demonstrate that small proteins encoded by rORFs display specific subcellularlocalizations,and confirm the presence of rORF2 in infected cells through mass spectrometry *** protein encoded by TuMV rORF2 forms punctuate granules that are localized in the perinuclear region and co-localized with viral replication *** rORF2 protein can directly interact with the viral RNA-dependent RNA polymerase,and mutation of rORF2 completely abolishes virus infection,whereas ectopic expression of rORF2 rescues the mutant ***,we show that several rORFs in the-RNA of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)have the ability to suppress type l interferon production and facilitate the infection of ve-sicular stomatitis *** addition,we provide evidence that TuMV might utilize internal ribosome entry sites to translate these small *** together,these findings indicate that the-RNA of+ssRNA vi-ruses can also have the coding capacity and that small proteins encoded therein play critical roles in viral infection,revealing a viral proteome larger than previously thought.

关键词： positive-sense single-stranded RNA viruses small proteins negative-sense RNA turnip mosaic virus severe acute respiratory syndrome coronavirus 2

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含多处裂纹梁的振动分析

应用力学学报 2007年第1期24卷 66-68页

作者：李学平余志武中南大学长沙410075

基于传递矩阵方法,提出了一种计算含有任意处裂纹梁固有频率的新方法。将梁内裂纹模拟为无质量的弯曲弹簧,得到了梁的解析特征方程。通过数值模拟计算,讨论了裂纹数量,以及裂纹位置和裂纹深度对梁的固有频率的影响。通过与文献[4]的计... 详细信息

基于传递矩阵方法,提出了一种计算含有任意处裂纹梁固有频率的新方法。将梁内裂纹模拟为无质量的弯曲弹簧,得到了梁的解析特征方程。通过数值模拟计算,讨论了裂纹数量,以及裂纹位置和裂纹深度对梁的固有频率的影响。通过与文献[4]的计算结果比较,验证了本文方法的有效性。

关键词：多处裂纹梁传递矩阵法振动分析