检索结果-南通市图书馆

Seasonal variation and sources of atmospheric polycyclic aromatic hydrocarbons in a background site on the Tibetan Plateau

引用

Journal of Environmental Sciences 2023年第3期125卷 524-532页

作者： xuemei wu Weijun Sun Baojuan Huai LeiWang Chuntan Han Yetang Wang Wenying Mi College of Geography and Environment Shandong Normal UniversityJinan 250014China Qilian Alpine Ecology and Hydrology Research Station Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China MINJIE Institute of Environmental Science and Health Research Geesthacht 21502Germany

The study of atmospheric polycyclic aromatic hydrocarbons(PAHs) in northeastern Tibetan Plateau with fragile ecological environment and complex atmospheric circulation system is blank. To understand the characteristics and sources of persistent organic pollutants in the atmosphere of the northeastern Tibetan Plateau, we monitored levels in the central Qilian Mountain. From 2016 to 2017, we collected 45-pair(particle + gas) samples using active air samplers to investigate the sources, transport paths, and their influencing factors.Sources of PAHs were analysed with a source diagnostic model, and atmospheric transport paths were calculated. The concentration range for Σ15PAHs was 439–4666 pg/m^(3), and the average was 2015 pg/m^(3). The PAHs in central Qilian Mountain are mainly low molecular weight(LMW) PAHs. Winter concentrations of PAHs were higher than those in summer. The transport of PAHs is mainly affected by westerlies, and there are seasonal differences. Source analysis showed that PAHs mainly came from coal and biomass combustion and vehicle emissions, with seasonal differences. This study clarifies the concentration and seasonal variation of PAHs in the northern Tibetan Plateau, which is conducive to understanding the atmospheric transport process and fate of pollutants. The background site of Qilian Mountains located in the Silk Road economic belt has the value and significance of longterm observation of pollutants.

关键词： Long-range atmospheric transport Seasonal variation concentration Meteorological factors Qilian Mountain

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Three novel alleles of OsGS1 developed by base-editing-mediated artificial evolution confer glufosinate tolerance in rice

引用

The Crop Journal 2023年第2期11卷 661-665页

作者： Bin Ren Yongjie Kuang Ziyan Xu xuemei wu Dawei Zhang Fang Yan Xiangju Li Xueping Zhou Guirong Wang Huanbin Zhou Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 440307GuangdongChina State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Scientific Observing and Experimental Station of Crop Pests in Guilin Ministry of Agriculture and Rural AffairsGuilin 541399GuangxiChina State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058ZhejiangChina Ministry of Education Key Laboratory of Bio-Resource and Eco-Environment College of Life SciencesSichuan UniversityChengdu 610065SichuanChina

Only few glufosinate-tolerant genes,such as phosphinothricin acetyltransferase(PAT)and bialaphos resistance(bar)identified from Streptomyces,are currently available for developing genetically modified rice in agricultural application.Following the rapid development of genome editing technology,generation of novel glufosinate-tolerant gene resources through artificial evolution of endogenous genes is more promising and highly desirable in rice molecular breeding program.In this study,the endogenous Glutamine synthetase1(OsGS1)was artificially evolved by base-editing-mediated gene evolution(BEMGE)in rice cells to create novel alleles conferring glufosinate tolerance in rice germplasms.Two novel glufosinate-tolerant OsGS1 alleles(OsGS1-AVPS and OsGS1-+AF)and one reported tolerant allele(OsGS1-SGTA)were successfully identified from approximately 4200 independent hygromycin-tolerant calli.Germination assays and spray tests revealed that these three OsGS1 alleles confer glufosinate tolerance in rice.Furthermore,OsGS1-AVPS and OsGS1-SGTA were quickly deployed into the elite rice cultivar Nangeng 46 through precise base editing.Overall,our results demonstrate the feasibility of developing glufosinate-tolerant rice by editing an endogenous rice gene in molecular breeding programs.

关键词： Base editing Gene evolution Glutamine synthetase Glufosinate tolerance Rice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Adenine base editor incorporating the N-methylpurine DNA glycosylase MPGv3 enables efficient A-to-K base editing in rice

引用

Plant Communications 2023年第6期4卷 230-233页

作者： xuemei wu Bin Ren Lang Liu Shengqun Qiu Xin’ge Li Peijing Li Fang Yan Honghui Lin Xueping Zhou Dawei Zhang Huanbin Zhou Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment College of Life ScienceState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Scientific Observing and Experimental Station of Crop Pests in Guilin Ministry of Agriculture and Rural AffairsGuilin 541399China Department of Plant Pathology China Agricultural UniversityBeijing 100193China State Key Laboratory of North China Crop Improvement and Regulation College of Plant ProtectionHebei Agricultural UniversityBaoding 071000China School of Life Science and Technology Hubei Engineering UniversityXiaogan 432000China State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China Hainan Yazhou Bay Seed Lab/National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China

Dear Editor,Crop genetic diversity and elite agronomic traits are mainly caused by genetic variants,approximately half of which are single-nucleotide polymorphisms.Precise nucleotide substitution through CRISPR–Cas-mediated base editors has been widely used to correct defective alleles and create novel alleles by artificial evolution for rapid crop genetic improvement.Since 2017,cytosine base editors(CBEs)and adenine base editors(ABEs)have been successively developed in many plant species and have been continuously optimized to generate highly efficient C-to-T and A-to-G transitions,as well as by-product C-to-A/G conversion with low efficiency(Ren et al.,2018;Yan et al.,2021).

关键词： elite base editor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sample Collection,DNA Extraction,and Library Construction Protocols of the Human Microbiome Studies in the International Human Phenome Project

引用

Phenomics 2023年第3期3卷 300-308页

作者： Yetong Wang Ruyi Zhang Yanni Pu Danqi Wang Yanren Wang xuemei wu Yujie Pan Chen Luo Guoping Zhao Zhexue Quan Yan Zheng State Key Laboratory of Genetic Engineering Human Phenome Instituteand School of Life SciencesFudan University2005 Songhu RoadShanghai 200433China Department of Cardiology Shanghai Institute of Cardiovascular DiseaseZhongshan HospitalFudan University1609 Xietu RoadShanghai 200032China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity ScienceSchool of Life SciencesFudan University2005 Songhu RoadShanghai 200433China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China

The human microbiome plays a crucial role in human health.In the past decade,advances in high-throughput sequencing technologies and analytical software have significantly improved our knowledge of the human microbiome.However,most studies concerning the human microbiome did not provide repeatable protocols to guide the sample collection,handling,and processing procedures,which impedes obtaining valid and timely microbial taxonomic and functional results.This protocol provides detailed operation methods of human microbial sample collection,DNA extraction,and library construction for both the amplicon sequencing-based measurements of the microbial samples from the human nasal cavity,oral cavity,and skin,as well as the shotgun metagenomic sequencing-based measurements of the human stool samples among adult par-ticipants.This study intends to develop practical procedure standards to improve the reproducibility of microbiota profiling of human samples.

关键词： Sample collection DNA extraction Library construction Human microbiome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sulforaphane protects primary cultures of cortical neurons against injury induced by oxygen-glucose deprivation/reoxygenation via anti-apoptosis

引用

Neuroscience Bulletin 2012年第5期28卷 509-516页

作者： xuemei wu Jing Zhao Shanshan Yu Yanlin Chen , Jingxian wu Yong Zhao Department of Pathology Department of Pathophysiology Chongqing Medical University Chongqing 400016 China

Objective To determine whether sulforaphane （SFN） protects neurons against injury caused by oxygen-glucose deprivation/reoxygenation （OGD/R） and, if so, to investigate the possible mechanisms. Methods Primary cultures of neurons were prepared from the cerebral cortex of 1-day-old Sprague-Dawley rats. On days 5-6 in vitro, the neurons were exposed to OGD for 1 h, followed by reoxygenation for 24 h. Cells were treated with 0, 0.1, 0.2, 0.5, 1, 2.5, or 5 μmol/L SFN, with or without 10 μmol/L LY294002, a PI3K-specific inhibitor, during OGD/R （a total of 25 h）. After 24-h reoxygenation, MTT was used to assess viability and injury was assessed by Hoechst 33258/propidium iodide （PI） staining; immunofluorescence staining and Western blot were performed to detect molecular events associated with apoptosis. Results The MTT assay showed that 1 μmol/L SFN significantly increased viability, and Hoechst 33258/PI staining showed that the numbers of injured neurons were reduced significantly in the SFN group. Furthermore, immunofluorescence staining and Western blot showed that SFN increased Bcl-2 and decreased cleaved caspase-3 levels. Moreover, LY294002 inhibited the phosphorylated-Akt expression evoked by SFN, decreased Bcl-2 expression and increased cleaved caspase-3 expression. Conclusion SFN protects neurons against injury from OGD/R and this effect may be partly associated with an anti-apoptosis pathway.

关键词： sulforaphane oxygen-glucose deprivation apoptosis neuroprotection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A point mutation of PRL gene in pituitary prolactin- secreting adenoma induced by 17-β-estradiol in SD rats

引用

Chinese Science Bulletin 2000年第5期45卷 435-439页

作者： xuemei wu Rongkun Xu Department of Physiology Institute of Basic Medical Science Chinese Academy of Medical Sciences Beijing China

Animal model bearing pituitary prolactin-secreting adenomas (prolactinoma) induced by 17-β-estradiol (E2) in both eutopic pituitary and ectopic pituitary grafted under the renal capsule was generated. Northern blotting assay indicated that PRL mRNA level in eutopic prolactinomas was higher than that in normal pituitaries and ectopic prolactinomas (P<0.05 - 0.01). By polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) analysis and DNA sequencing, a point mutation from C to A occurring at -36 nt in proximal promoter of rat PRL (rPRL) gene was found only in eutopic prolactinomas. No base change was detected in ectopic prolactinomas. Fusion gene transfection assay in vitro exhibited increased activity of the mutant promoter derived from eutopic prolactinoma (P<0.01). These data suggested that the base change in the proximal promoter of rPRL gene may be associated with hyperexpression of rPRL gene in eutopic prolactinomas. The pathogenesis of eutopic and ectopic prolactinomas

关键词： PCR-SSCP prolactin-secreting adenoma rPRL gene DNA sequencing point mutation.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The control and optimization of macro/micro-structure of ion conductive membranes for energy conversion and storage

引用

Chinese Journal of Chemical Engineering 2016年第5期24卷 558-571页

作者： Xiaoming Yan Wenji Zheng Xuehua Ruan Yu Pan xuemei wu Gaohong He State Key Laboratory of Fine Chemicals School of Petroleum and Chemical EngineeringDalian University of Technology Research and Development Center of Membrane Science and Technology School of Chemical EngineeringDalian University of Technology

Ion conductive membranes(ICMs)are frequently used as separators for energy conversion and storage technologies of fuel cells,flow battery,and hydrogen pump,because of their good ion-selective conduction and low electronic conductivity.Firstly,this feature article reviews the recent studies on the development of new nonfluorinated ICMs with low cost and their macro/micro-structure control.In general,these new nonfluorinated ICMs have lower conductivity than commercial perfluorinated ones,due to their poor ion transport channels.Increasing ion exchange capacity(IEC)would create more continuous hydrophilic channels,thus enhancing the conductivity.However,high IEC also expands the overall hydrophilic domains,weakens the interaction between polymer chains,enhances the mobility of polymer chains,and eventually induces larger swelling.The micro-scale expansion and macro-scale swelling of the ICMs with high IEC could be controlled by limiting the mobility of polymer chains.Based on this strategy,some ef ficient techniques have been developed,including covalent crosslinking,semi-interpenatrating polymer network,and blending.Secondly,this review introduces the optimization of macro/microstructure of both perfluorinated and nonfluorinated ICMs to improve the performance.Macro-scale multilayer composite is an ef ficient way to enhance the mechanical strength and the dimensional stability of the ICMs,and could also decrease the content of per fluorosulfonic acid resin in the membrane,thereby reducing the cost of the perfluorinated ICMs.Long side chain,multiple functionalization,small molecule inducing micro-phase separation,electrospun nano fiber,and organic–inorganic hybrid could construct more ef ficient ion transport channels,improving the ion conductivity of ICMs.

关键词：离子传导存储技术能量转换导电膜宏观控制优化微结构

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Catalytic hydrogenation performance of ZIF-8 carbide for electrochemical reduction of carbon dioxide

引用

Chinese Journal of Chemical Engineering 2021年第11期34卷 144-153页

作者： Shuai Fan Huiyuan Cheng Manman Feng xuemei wu Zihao Fan Dongwei Pan Gaohong He State Key Laboratory of Fine Chemicals Research and Development Center of Membrane Science and TechnologySchool of Chemical EngineeringDalian University of TechnologyDalian 116024China

The conversion of CO_(2) electrocatalytic hydrogenation into energy-rich fuel is considered to be the most effective way to carbon recycle.Nitrogen-doping carbonized ZIF-8 is proposed as carrier of the earth-rich Sn catalyst to overcome the limit of electron transfer and CO_(2) adsorption capacity of Sn.Hierarchically porous structure of Sn doped carbonized ZIF-8 is controlled by hydrothermal and carbonization conditions,which induces much higher specific surface area than that of the commercial Sn nanoparticle(1003.174 vs.7.410 m^(2)·g^(-1)).The shift of nitrogen peaks in X-ray Photoelectron Spectroscopy spectra indicates interaction between ZIF-8 and Sn,which induces the shift of electron cloud from Sn to the chemical nitrogen to enhance conductivity and regulate electron transfer from catalyst to CO_(2).Lower mass transfer resistance and Warburg resistance are investigated through EIS,which significantly improves the catalytic activity for CO_(2) reduction reaction(CO_(2)RR).Onset potential of the reaction is reduced from-0.74 V to less than-0.54 V vs.RHE.The total Faraday efficiency of HCOOH and CO reaches 68.9%at-1.14 V vs.RHE,which is much higher than that of the commercial Sn(45.0%)and some other Sn-based catalyst reported in the literature.

关键词： Carbon dioxide Electrochemistry Selective catalytic reduction Electrochemical hydrogen pump Nitrogen-doping carbonized ZIF-8

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Construction of regulatory network for alopecia areata progression and identification of immune monitoring genes based on multiple machine-learning algorithms

引用

Precision Clinical Medicine 2023年第2期6卷 67-80页

作者： Jiachao Xiong Guodong Chen Zhixiao Liu xuemei wu Sha Xu Jun Xiong Shizhao Ji Minjuan wu Department of Histology and Embryology Naval Military Medical UniversityShanghai 200433China Department of Plastic Surgery Shanghai East HospitalTongji University School of MedicineShanghai 200120China Institute of Translational Medicine Naval Military Medical UniversityShanghai 200433China Department of Burn Surgery The First Affiliated Hospital of Naval Medical UniversityShanghai 200433China

Objectives Alopecia areata(AA)is an autoimmune-related non-cicatricial alopecia,with complete alopecia(AT)or generalized alopecia(AU)as severe forms of AA.However,there are limitations in early identification of AA,and intervention of AA patients who may progress to severe AA will help to improve the incidence rate and prognosis of severe AA.Methods We obtained two AA-related datasets from the gene expression omnibus database,identified the differentially expressed genes(DEGs),and identified the module genes most related to severe AA through weighted gene co-expression network analysis.Functional enrichment analysis,construction of a protein–protein interaction network and competing endogenous RNA network,and immune cell infiltration analysis were performed to clarify the underlying biological mechanisms of severe AA.Subsequently,pivotal immune monitoring genes(IMGs)were screened through multiple machine-learning algorithms,and the diagnostic effectiveness of the pivotal IMGs was validated by receiver operating characteristic.Results A total of 150 severe AA-related DEGs were identified;the upregulated DEGs were mainly enriched in immune response,while the downregulated DEGs were mainly enriched in pathways related to hair cycle and skin development.Four IMGs(LGR5,SHISA2,HOXC13,and S100A3)with good diagnostic efficiency were obtained.As an important gene of hair follicle stem cells stemness,we verified in vivo that LGR5 downregulation may be an important link leading to severe AA.Conclusion Our findings provide a comprehensive understanding of the pathogenesis and underlying biological processes in patients with AA,and identification of four potential IMGs,which is helpful for the early diagnosis of severe AA.

关键词： alopecia areata immune response machine learning immune monitoring genes diagnosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High magnetic field helicon plasma discharge for plasma-wall interaction studies

引用

Science China(Physics,Mechanics & Astronomy) 2016年第4期59卷 88-89页

作者： TianYuan Huang ChengGang Jin Jun Yu xuemei wu LanJian Zhuge College of Physics Optoelectronics and Energy & Collaborative Innovation Center of Suzhou Nano Science and TechnologySoochow University Key Lab of Advanced Optical Manufacturing Technologies of Jiangsu Province & Key Lab of Modern Optical Technologies of Education Ministry of China Soochow University Analysis and Testing Center Soochow University

Dear Editors,Plasma-Wall Interactions(PWIs)are important for the development of magnetic confinement fusion devices such as International Thermonuclear Experimental Reactor(ITER),as they can release impurities into th... 详细信息

关键词：放电等离子体相互作用螺旋波等离子体高磁场国际热核实验反应堆核聚变装置 ITER 磁约束

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：