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Guidelines for application of high-content screening in traditional Chinese medicine:concept,equipment,and troubleshooting

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Acupuncture and Herbal Medicine 2024年第1期4卷 1-15页

作者： xuechun chen Lu Li Mingxu Zhang Jian Yang ChunMing Lyu Yizhou Xu Yang Yang Yi Wang Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhouChina Department of Cardiology Hangzhou First People’s HospitalHangzhouChina National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang UniversityJiaxingChina Molecular Devices Shanghai Corporation ShanghaiChina National Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese MedicineTianjinChina Science and Technology Experiment Center Shanghai University of Traditional Chinese MedicineShanghaiChina Engineering Research Center of Innovative Anticancer Drugs Ministry of Educationhangzhou China

High-content screening(HCS)technology combines automated high-speed imaging hardware and single-cell quantitative analysis.It can greatly accelerate data acquisition in cellular fluorescence imaging and is a powerful research technique in traditional Chinese medicine(TCM).An increasing number of laboratories and platforms,including TCM laboratories,have begun utilizing HCS systems.However,this technology is still in its infancy in TCM research and there is a lack of sufficient experience with the associated concepts,instrument configurations,and analysis methods.To improve the understanding of HCS among researchers in the field of TCM,this paper summarizes the concept of HCS,software and hardware configuration,the overall research process,as well as common problems and related solutions of HCS in TCM research based on our team’s previous research experience,providing several research examples and an outlook on future perspectives,aiming to provide a technical guide for HCS in TCM research.

关键词： High-content imaging High-content screening Traditional Chinese medicine

Deciphering bioactive compounds of complex natural products by tandem mass spectral molecular networking combined with an aggregation-induced emission based probe

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Journal of Pharmaceutical Analysis 2022年第1期12卷 129-135页

作者： Zhenzhong Yang Jun Li xuechun chen Xiaoping Zhao Yi Wang Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou310058China College of Preclinical Medicine Zhejiang Chinese Medical UniversityHangzhou310053China

Natural products are great treasure troves for the discovery of bioactive components.Current bioassay guided fractionation for identification of bioactive components is time-and workload-consuming.In this study,we proposed a robust and convenient strategy for deciphering the bioactive profile of natural products by mass spectral molecular networking combined with rapid bioassay.As a proof-of-concept,the strategy was applied to identify angiotensin converting enzyme(ACE)inhibitors of Fangjihuangqi decoction(FJHQD),a traditional medicine clinically used for the treatment of heart failure.The chemical profile of FJHQD was comprehensively revealed with the assistance of tandem mass spectral molecular networking,and a total of 165 compounds were identified.With characterized constituents,potential clinical applications of FJHQD were predicted by Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine,and a range of cardiovascular related diseases were significantly enriched.ACE inhibitory activities of FJHQD and its constituents were then investigated with an aggregation-induced emission based fluorescent probe.FJHQD exhibited excellent ACE inhibitory effects,and a bioactive molecular network was established to elucidate the ACE inhibitory profile of constituents in FJHQD.This bioactive molecular network provided a panoramic view of FJHQD’s ACE inhibitory activities,which demonstrated that flavones from Astragali Radix and Glycyrrhizae Radix et Rhizoma,saponins from Astragali Radix,and sesquiterpenoids from Atractylodis Macrocephalae Rhizoma were principal components responsible for this effect of FJHQD.Among them,four novel ACE inhibitors were the first to be reported.Our study indicated that the proposed strategy offers a useful approach to uncover the bioactive profile of traditional medicines and provides a pragmatic workflow for exploring bioactive components.

关键词： Bioactive profile Bioactive molecular network Fangjihuangqi decoction Angiotensin converting enzyme inhibitors

Wave effect of gravitational waves intersected with a microlens field:A new algorithm and supplementary study

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Science China(Physics,Mechanics & Astronomy) 2023年第3期66卷 156-168页

作者： Xikai Shan Guoliang Li xuechun chen Wenwen Zheng Wen Zhao Department of Astronomy Beijing Normal UniversityBeijing 100875China Purple Mountain Observatory Chinese Academy of SciencesNanjing 210023China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China CAS Key Laboratory for Researches in Galaxies and Cosmology Department of AstronomyUniversity of Science and Technology of ChinaChinese Academy of SciencesHefei 230026China

The increase in gravitational wave(GW) events has allowed receiving strong lensing image pairs of GWs. However, the wave effect(diffraction and interference) due to the microlens field contaminates the parameter estimation of the image pair, which may lead to a misjudgment of strong lensing signals. To quantify the influence of the microlens field, researchers need a large sample of statistical research. Nevertheless, due to the oscillation characteristic, the Fresnel-Kirchhoff diffraction integral’s computational time hinders this aspect’s study. Although many algorithms are available, most cannot be well applied to the case where the microlens field is embedded in galaxy/galaxy clusters. This work proposes a faster and more accurate algorithm for studying the wave optics effect of microlenses embedded in different types of strong lensing images. Additionally, we provide a quantitative estimation criterion for the lens plane boundary for the Fresnel-Kirchhoff diffraction integral. This algorithm can significantly facilitate the study of wave optics, particularly in the case of microlens fields embedded in galaxy/galaxy clusters.

关键词： gravitational waves gravitational micro lensing Fresnel-Kirchhoff diffraction integral

Experimental investigation of the stimulated Raman scattering effect in high-power nanosecond superfluorescent fiber source

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High Power Laser Science and Engineering 2023年第6期11卷 229-238页

作者： Chaoyu Ning Shuzhen Zou Haijuan Yu Jiexi Zuo xuechun chen Shuang Xu Shifei Han Xinyao Li Wenjuan Wu Chaojian He xuechun Lin Laboratory of All-Solid-State Light Sources Institute of SemiconductorsChinese Academy of SciencesBeijingChina Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijingChina College of Materials Science and Opto-Electronic Technology University of Chinese Academy of SciencesBeijingChina Beijing Engineering Technology Research Center of All-Solid-State Lasers Advanced Manufacturing BeijingChina

In this work,we experimentally investigate the dependence of the stimulated Raman scattering(SRS)effect on the seed linewidth of a high-power nanosecond superfluorescent fiber source(ns-SFS).The results reveal that the SRS in the ns-SFS amplifier is significantly influenced by the full width at half maximum(FWHM)of the ns-SFS seed,and there is an optimal FWHM linewidth of 2 nm to achieve the lowest SRS in our case.The first-order SRS power ratio increases rapidly when the seed’s linewidth deviates from the optimal FWHM linewidth.By power scaling the ns-SFS seed with the optimal FWHM linewidth,a narrowband all-fiberized ns-SFS amplifier is achieved with a maximum average power of 602 W,pulse energy of 24.1 mJ and corresponding peak power of 422.5 kW.This is the highest average power and pulse energy achieved for all-fiberized ns-SFS amplifiers to the best of our knowledge.

关键词： fiber amplifier high power nanosecond superfluorescent fiber source stimulated Raman scattering effect

Rapid and mass manufacturing of soft hydrogel microstructures for cell patterns assisted by 3D printing

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Bio-Design and Manufacturing 2022年第4期5卷 641-659页

作者： Chaofan He xuechun chen Yuan Sun Mingjun Xie Kang Yu Jing He Jinwei Lu Qing Gao Jing Nie Yi Wang Yong He State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027China Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Department of Orthopedic Surgery The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China Key Laboratory of Materials Processing and Mold Zhengzhou UniversityZhengzhou 450002China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310027China Cancer Center Zhejiang UniversityHangzhou 310058China State Key Laboratory of Component-Based Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 300072China

Micro-/nano-patterns on hydrogels are widely used in cell patterning.However,manufacturing molds with traditional lithography is time-consuming and expensive.In addition,the excessive demolding force can easily damage patterns since biocompatible hydrogels are ultra-soft or brittle.Here,we presented a novel method for rapid and mass fabrication of cell patterns.High-precision three-dimensional(3D)printing was used to manufacture a mold with a resolution of 2µm,and the gelatin-based hydrogel was cured by thermal–photo-crosslinking so that the low-concentration and low-substitutionrate hydrogel could be demolded successfully.We found that pre-cooling before illumination made gelatin-based hydrogels resilient due to the partial regain of triple-helix structures.With this method,arbitrarily customized hydrogel patterns with a feature size of 6–80µm can be fabricated stably and at low cost.When cardiomyocytes were seeded on ultra-soft hydrogels with parallel groove structures,a consistent and spontaneous beating with 216 beats per minute(BPM)could be observed,approaching the natural beating rate of rat hearts(300 BPM).Overall,this work provides a general scheme for manufacturing cell patterns which has great potential for cell ethology and tissue repair.

关键词： 3D printing Gelatin-based hydrogel Cell pattern Cardiomyocytes beating

Theoretical Analysis of Random Scattering Induced by Microlensing

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Research in Astronomy and Astrophysics 2023年第8期23卷 178-187页

作者： Wenwen Zheng Hou-Zun chen xuechun chen Guoliang Li Purple Mountain Observatory Chinese Academy of SciencesNanjing 210023China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China Institute for Frontier in Astronomy and Astrophysics Beijing Normal UniversityBeijing 102206China Department of Astronomy Beijing Normal UniversityBeijing 100875China

Theoretical investigations into the deflection angle caused by microlenses offer a direct path to uncovering principles of the cosmological microlensing effect.This work specifically concentrates on the the probability density function(PDF)of the light deflection angle induced by microlenses.We have made several significant improvements to the widely used formula from Katz et al.First,we update the coefficient from 3.05 to 1.454,resulting in a better fit between the theoretical PDF and our simulation results.Second,we developed an elegant fitting formula for the PDF that can replace its integral representation within a certain accuracy,which is numerically divergent unless arbitrary upper limits are chosen.Third,to facilitate further theoretical work in this area,we have identified a more suitable Gaussian approximation for the fitting formula.

关键词： gravitational lensing micro-methods analytical-galaxies stellar content

1.2 kW all-fiber narrow-linewidth picosecond MOPA system

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High Power Laser Science and Engineering 2023年第2期11卷 52-58页

作者： Jiexi Zuo Haijuan Yu Shuzhen Zou Zhiyong Dong Chaojian He Shuang Xu Chaoyu Ning xuechun chen Xinyao Li xuechun Lin Laboratory of All-Solid-State Light Sources Institute of SemiconductorsChinese Academy of SciencesBeijingChina Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijingChina College of Materials Science and Opto-Electronic Technology University of Chinese Academy of SciencesBeijingChina Beijing Engineering Technology Research Center of All-Solid-State Lasers Advanced Manufacturing BeijingChina

Achieving an all-fiber ultra-fast system with above kW average power and mJ pulse energy is extremely challenging.This paper demonstrated a picosecond monolithic master oscillator power amplifier system at a 25 MHz repetition frequency with an average power of approximately 1.2 kW,a pulse energy of approximately 48μJ and a peak power of approximately 0.45 MW.The nonlinear effects were suppressed by adopting a dispersion stretched seed pulse(with a narrow linewidth of 0.052 nm)and a multi-mode master amplifier with an extra-large mode area;then an ultimate narrow bandwidth of 1.32 nm and a moderately broadened pulse of approximately 107 ps were achieved.Meanwhile,the great spatio-temporal stability was verified experimentally,and no sign of transverse mode instability appeared even at the maximum output power.The system has shown great power and energy capability with a sacrificed beam propagation product of 5.28 mm·mrad.In addition,further scaling of the peak power and pulse energy can be achieved by employing a lower repetition and a conventional compressor.

关键词： fiber laser nonlinear optics picosecond pulse transverse mode instability

新课标背景下的跨学科学习:现实阻滞与纾解路径

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教育研究与实验 2024年第2期 73-81页

作者：刘志军陈雪纯河南大学教育学部开封475004 河南大学教育考试与评价研究院

跨学科学习是《义务教育课程方案和课程标准(2022年版)》中的一大亮点,但并非课程改革历程中的新事物,它所蕴含的跨学科知识统整思维在以往课标的改革举措中已有所体现。在新课标背景下,核心素养育人目标及理念的明确,赋予了跨学科学习... 详细信息

跨学科学习是《义务教育课程方案和课程标准(2022年版)》中的一大亮点,但并非课程改革历程中的新事物,它所蕴含的跨学科知识统整思维在以往课标的改革举措中已有所体现。在新课标背景下,核心素养育人目标及理念的明确,赋予了跨学科学习实践更丰富、更深刻的内涵及价值。作为核心素养导向下学习方式变革的创新实践,跨学科学习真正落地需以课程、教学及评价的整体性变革为牵引。当前实践中跨学科学习面临着课程结构学科化、教学设计还原化以及评价生态功利化等困境,其综合性、实践性、发展性价值难以充分发挥。为推动核心素养导向下跨学科学习实践落地,需要形成核心素养为目标的主题式课程结构,展开深度学习为基准的大概念项目式教学设计,实施素养发展为导向的表现性学习评价,以及建设多元支持协同的保障机制。

关键词：跨学科学习新课程标准核心素养深度学习

达格列净治疗糖尿病合并消化性溃疡的价值研究

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糖尿病新世界 2024年第7期27卷 90-92,96页

作者：林秋龙陈学淳闽侯县人民医院内科福建福州350119

目的分析达格列净治疗糖尿病合并消化性溃疡的价值。方法选取2022年9月—2023年8月闽侯县人民医院收治的86例糖尿病合并消化性溃疡患者为研究对象,采用随机数表法分为两组,各43例。对照组使用二甲双胍片治疗,研究组在对照组基础上联合... 详细信息

目的分析达格列净治疗糖尿病合并消化性溃疡的价值。方法选取2022年9月—2023年8月闽侯县人民医院收治的86例糖尿病合并消化性溃疡患者为研究对象,采用随机数表法分为两组,各43例。对照组使用二甲双胍片治疗,研究组在对照组基础上联合达格列净治疗。对比两组血糖指标水平、临床疗效、不良事件发生情况、生活质量。结果治疗后,研究组血糖指标、临床疗效、生活质量均优于对照组,差异有统计学意义(P均0.05)。结论达格列净治疗糖尿病合并消化性溃疡,能够降低血糖水平,提高疗效与生活质量,且安全性较高。

关键词：达格列净糖尿病消化性溃疡疗效血糖不良事件

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