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Sustained-release genistein from nanostructured lipid carrier suppresses human lens epithelial cell growth

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International Journal of Ophthalmology(English edition) 2016年第5期9卷 643-649页

作者： Jin-Lu liu Wen-Ji Zhang xue-Dong li Na Yang Wei-San Pan Jun Kong Jin-Song Zhang Department of Ophthalmology the Fourth Affiliated Hospital of China Medical UniversityEye Hospital of China Medical UniversityKey Lens Research Laboratory of Liaoning Province Department of Pharmaceutics School of PharmacyShenyang Pharmaceutical University

AIM： To design and investigate the efficacy of a modified nanostructured lipid carrier loaded with genistein（Gen-NLC） to inhibit human lens epithelial cells（HLECs） proliferation.·METHODS： Gen-NLC was made by melt emulsification method. The morphology, particle size（PS）, zeta potentials（ZP）, encapsulation efficiency（EE） and in vitro release were characterized. The inhibition effect of nanostructured lipid carrier（NLC）, genistein（Gen） and Gen-NLC on HLECs proliferation was evaluated by cell counting kit-8（CCK-8） assay, gene and protein expression of the proliferation marker Ki67 were evaluated with real-time quantitative polymerase chain reaction（RT-q PCR） and immunofluorescence analyses.·RESULTS： The mean PS of Gen-NLC was 80.12±1.55 nm with a mean polydispersity index of 0.11±0.02. The mean ZP was-7.14 ±0.38 m V and the EE of Gen in the nanoparticles was 92.3% ±0.73%. Transmission electron microscopy showed that Gen-NLC displayed spherical-shaped particles covered by an outer-layer structure. In vitro release experiments demonstrated a prolonged drug release for 72 h. The CCK-8 assay results showed the NLC had no inhibitory effect on HLECs and Gen-NLC displayed a much more prominent inhibitory effect on cellular growth compared to Gen of the same concentration. The m RNA and protein expression of Ki67 in LECs decreased significantly in Gen-NLC group.·CONCLUSION： Sustained drug release by Gen-NLCs may impede HLEC growth.

关键词： posterior capsular opacification genistein nanostructured lipid carrier human lens epithelial cells

Value of D-Dimers in patients with acute aortic dissection

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Journal of Nanjing Medical University 2007年第2期21卷 86-88页

作者： Chuncai xue Yigang li Department of Cardiology the People ＇s Hospital of Binzhou Binzhou 256610 China Department of Cardiology Xinhua hospital Shanghai JiaoTong university Shanghai 200092 China.

Objective： To evaluatel the value of D-dimers in patients with acute aortic dissection （AAD）. Methods： This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimers were measured by Sta-liatest D-D1 immunoturbidimetric assay. Results： D-dimer level was higher （P 〈 0.001） in patients with AAD（7.91 ± 5.52 μg/ml） than that in non- AAD group（1.57± 1.24 μg/ml）. D-dimer was positive （〉0.4 μg/ml） in all patients with AAD and in 10 control group patients （37%）. Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B （8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P 〈 0.01）. D-dimer values tended to be higher in more extended disease（3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P 〈 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ）. Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion： D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.

关键词： D-dimer aorta dissection coagulation

Targeted delivery of a STING agonist to brain tumors using bioengineered protein nanoparticles for enhanced immunotherapy

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Bioactive Materials 2022年第10期7卷 232-248页

作者： Bin Wang Maoping Tang Ziwei Yuan Zhongyu li Bin Hu Xin Bai Jinxian Chu Xiaoyang Xu xue-Qing Zhang Engineering Research Center of Cell&Therapeutic Antibody Ministry of EducationSchool of PharmacyShanghai Jiao Tong University800 Dongchuan RoadShanghai200240PR China Department of Chemical and Materials Engineering New Jersey Institute of TechnologyNewarkNJ07102USA

Immunotherapy is emerging as a powerful tool for combating many human ***,the application of this life-saving treatment in serious brain diseases,including glioma,is greatly *** major obstacle is the lack of effective technologies for transporting therapeutic agents across the blood-brain barrier(BBB)and achieving targeted delivery to specific cells once across the ***,an iron storage protein,traverses the BBB via receptor-mediated transcytosis by binding to transferrin receptor 1(TfR1)overexpressed on BBB endothelial ***,we developed bioengineered ferritin nanoparticles as drug delivery carriers that enable the targeted delivery of a small-molecule immunomodulator to achieve enhanced immunotherapeutic efficacy in an orthotopic glioma-bearing mouse *** fused different glioma-targeting moieties on self-assembled ferritin nanoparticles via genetic engineering,and RGE fusion protein nanoparticles(RGE-HFn NPs)were identified as the best ***,RGE-HFn NPs encapsulating a stimulator of interferon genes(STING)agonist(SR717@RGE-HFn NPs)maintained stable self-assembled structure and targeting properties even after traversing the *** the glioma-bearing mouse model,SR717@RGE-HFn NPs elicited a potent local innate immune response in the tumor microenvironment,resulting in significant tumor growth inhibition and prolonged ***,this biomimetic brain delivery platform offers new opportunities to overcome the BBB and provides a promising approach for brain drug delivery and immunotherapy in patients with glioma.

关键词： Blood brain barrier Bioengineered protein nanoparticles Dual-targeting property STING agonist Glioma-targeted immunotherapy

Massively prolapsed intervertebral disc herniation with interlaminar endoscopic spine system Delta endoscope: A case series

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World Journal of Clinical Cases 2021年第1期9卷 61-70页

作者： Sheng-Wei Meng Chen Peng Chuan-li Zhou Hao Tao Chao Wang Kai Zhu Meng-Xiong Song xue-XiaoMa Spinal Department The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina

BACKGROUND Surgery is often indicated for patients with massively prolapsed intervertebral disc *** interlaminar endoscopic spine system(iLESSYS)Delta 6-mm working channel endoscope has advantages over other *** aim of this study was to explore the benefits and complications of using the iLESSYS Delta for the treatment of massively prolapsed intervertebral disc *** To explore the clinical benefits of treating massively prolapsed lumbar intervertebral disc herniation with the iLESSYS Delta *** In this study,the data of 37 patients who underwent surgery with the iLESSYS Delta endoscope at The Affiliated Hospital of Qingdao University were retrospectively *** blood loss,operation time,and complications were *** visual analog scale(VAS),oswestry disability index(ODI),and modified MacNab criteria were determined before and at 1 d,3 mo,and 6 mo after *** The mean intraoperative blood loss was 20.4±1.2 *** mean operation time was 97.3±12.4 *** VAS scores for leg and back pain decreased from 68.0±7.3,34.4±8.5 before operation to 2.5±1.7,5.5±1.9 at 6 mo after surgery,*** ODI also decreased from 60.2±7.3 to 17.9±3.4 at 6 mo after *** improvement rate of the MacNab score was 86.4%,which was considered *** spinal dural injury,nerve root injury,secondary protrusion of intervertebral disc,or myeloid hypertension was found during *** The iLESSYS Delta 6-mm working channel endoscope has several advantages in terms of clinical and functional benefits,complications,and low risk of residual vertebral pulp in treating patients with massively prolapsed intervertebral disc herniation.

关键词： Endoscopy Lumbar disc herniation Minimally invasive Spine surgery Retrospective study

Effect of Traditional Chinese Medicine on the Growth and Invasion of Human First Trimester Cytotrophoblasts in vitro

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Journal of Reproduction and Contraception 2008年第2期19卷 73-82页

作者： Hai-yan WANG Qin-di ZOU xue-lian li Sui-qi GUI Gynecology ＆ Obstetrics Hospital Fudan University Shanghai 200011 China

Objective To investigate the effect of Traditional Chinese Medicine（TCM） on the growth and invasion of human first trimester cytotrophoblasts in vitro.Methods Serum contained drug was collected from rats adiministered with herbs intragastricly. Serum collected from rats administered with saline was used as the control. Isolated from first trimester placental tissues, human cytotrophoblast cultured in media with 5%, 10%, 20% drug-containing serum for a 24 h, 48 h or 72 h period. Then cell morphology was measured by scanning electric microscope, cell viability by MTT assay, cell DNA content by flow cytomester, and cell invasion by transwell assay.Results After a 48 h- and 72 h-culture period in media with drug-containing rat serum, cytotrophoblast showed more and longer cell pseudopodia than control cells. In addition, cytotrophoblast exhibited more cell microvillous than control cells after a 72 h culture with drug-containing serum. Cell viability cultured with drug-containing serum was higher than control cells. Cells cultured with drug-containing serum in S phase were more, by contrast, in sub-G1 and G2/M phase were less than the control. Cells cultured with drug-containing serum for 72 h showed significantly greater invasive ability than control cells.Conclusion TCM had a beneficial role in the growth and invasion of human first trimester cytotrophoblast in vitro.

关键词： Traditional Chinese Medicine(TCM) cytotrophoblast proliferation invasion

A compact inorganic layer for robust anode protection in lithium-sulfur batteries

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InfoMat 2020年第2期2卷 379-388页

作者： Yu-Xing Yao xue-Qiang Zhang Bo-Quan li Chong Yan Peng-Yu Chen Jia-Qi Huang Qiang Zhang Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijingChina Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijingChina

lithium-sulfur(li-S)batteries are one of the most promising candidates for high energy density rechargeable batteries beyond current li-ion ***,severe corrosion of li metal anode and low Coulombic efficiency(CE)induced by the unremitting shuttle of li polysulfides immensely hinder the practical applications of li-S ***,a compact inorganic layer(CIL)formed by ex situ reactions between li anode and ionic liquid emerged as an effective strategy to block li polysulfides and suppress shuttle effect.A CE of 96.7%was achieved in li-S batteries with CIL protected li anode in contrast to 82.4%for bare li anode while no lithium nitrate was ***,the corrosion of li during cycling was effectively *** applied to working batteries,80.6%of the initial capacity after 100 cycles was retained in li-S batteries with CIL-protected ultrathin(33μm)li anode compared with 58.5%for bare li anode,further demonstrating the potential of this strategy for practical *** study presents a feasible interfacial regulation strategy to protect li anode with the presence of li polysulfides and opens avenues for li anode protection in li-S batteries under practical conditions.

关键词： electrochemical energy storage lithium metal anode lithium-sulfur batteries rechargeable battery solid electrolyte interphase

Research on the Applications of Case Studies and Role Playing Methods on the Enterprise Strategic Management Course Teaching

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International Journal of Technology Management 2015年第11期 49-51页

作者： Jing xue Aining li Yantai Nanshan University Longkou City Shandong Province 265713

In this paper, we conduct research on the applications of case studies and role playing methods on the enterprise strategic management course teaching. Training is an important part of the enterprise human resources management. For enterprises is a kind of novel important human capital investment, as well as other capital investments, both benefits. Also be risky, because of the risks and benefits is coexistence, indivisible. To deal with the mentioned challenge, we combine the novel and state-of-the-art training methodology to maximize the effectiveness of the training process. In the article, we cast our new opinion together with the prospect for the future.

关键词： Enterprise Strategic Management Case Studies Role Playing Course and Education.

Apparent diffusion coefficient-based histogram analysis differentiates histological subtypes of periampullary adenocarcinoma

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World Journal of Gastroenterology 2019年第40期25卷 6116-6128页

作者： Jing-Yu Lu Hao Yu Xian-Lun Zou Zhen li xue-Mei Hu Ya-Qi Shen Dao-Yu Hu Department of Radiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina Department of Radiology the First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina

BACKGROUND For periampullary adenocarcinoma,the histological subtype is a better prognostic predictor than the site of tumor ***-type periampullary adenocarcinoma(IPAC)is reported to have a better prognosis than the pancreatobiliary-type periampullary adenocarcinoma(PPAC).However,the classification of histological subtypes is difficult to determine before *** diffusion coefficient(ADC)histogram analysis is a noninvasive,nonenhanced method with high reproducibility that could help differentiate the two *** To investigate whether volumetric ADC histogram analysis is helpful for distinguishing IPAC from *** Between January 2015 and October 2018,476 consecutive patients who were suspected of having a periampullary tumor and underwent magnetic resonance imaging(MRI)were reviewed in this retrospective *** patients who underwent MRI at 3.0 T with different diffusion-weighted images(b-values=800 and 1000 s/mm^2)and who were confirmed with a periampullary adenocarcinoma were further ***,the mean,5th,10th,25th,50th,75th,90th,and 95th percentiles of ADC values and ADCmin,ADCmax,kurtosis,skewness,and entropy were obtained from the volumetric histogram *** were made by an independent Student's t-test or Mann-Whitney U ***-class receiver operating characteristic curve analysis was performed to determine and compare the diagnostic value of each significant *** In total,40 patients with histopathologically confirmed IPAC(n=17)or PPAC(n=23)were *** mean,5th,25th,50th,75th,90th,and 95th percentiles and ADCmax derived from ADC1000 were significantly lower in the PPAC group than in the IPAC group(P<0.05).However,values derived from ADC800 showed no significant difference between the two *** 75th percentile of ADC1000 values achieved the highest area under the curve(AUC)for differentiating IPAC from PPAC(AUC=0.781;sensitivity,91%;specificity,59%;cut-off value,1.50×10^-3

关键词： Periampullary adenocarcinoma Apparent diffusion coefficient Histogram analysis Histopathology Differential diagnosis

Evaluation of the etiology and risk factors for maternal sepsis: A single center study in Guangzhou, China

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World Journal of Clinical Cases 2021年第26期9卷 7704-7716页

作者： lin lin Lu-Wen Ren xue-Yuan li Wen Sun Yan-Hong Chen Jing-Si Chen Dun-Jin Chen Department of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150Guangdong ProvinceChina Key Laboratories for Major Obstetric Diseases of Guangdong Province Guangzhou 510150Guangdong ProvinceChina Department of Fetal Medicine and Prenatal Diagnosis The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150Guangdong ProvinceChina

BACKGROUND Maternal sepsis is a major cause of gestational morbidity and neonatal mortality worldwide and particularly in *** To evaluate the etiology of maternal sepsis and further identify its risk *** In this retrospective study,we evaluated 70698 obstetric patients who were admitted to the Third Affiliated Hospital of Guangzhou Medical University between January 1,2009 and June 30,*** were divided into sepsis group and non-sepsis group based on the incidence of *** about medical history(surgical and obstetric history)and demographic information were *** Mann-Whitney U test was used to compare patient age,gestational age and duration of hospitalization between the two *** and multivariate logistic regression models were used to analyze the etiology and the risk factors for maternal *** and adjusted odds ratios(OR)are *** A total of 561 of 70698 obstetric patients were diagnosed with infection;of the infected patients,492 had non-sepsis associated infection(87.7%),while 69 had sepsis(12.3%).The morbidity rate of maternal sepsis was 9.76/10000;the fatality rate in the sepsis group was 11.6%(8/69).Emergency admission(OR=2.183)or transfer(OR=2.870),irregular prenatal care(OR=2.953),labor induction(OR=4.665),cervical cerclage(OR=14.214),first trimester(OR=6.806)and second trimester(OR=2.09)were significant risk factors for maternal *** Mode of admission,poor prenatal care,labor induction,cervical cerclage,first trimester and second trimester pregnancy were risk factors for maternal *** coli was the most common causative organism for maternal sepsis,and the uterus was the most common site of infection.

关键词： Maternal sepsis Sepsis-3 Infection Risk factors