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一种新的胰腺癌相关性自身抗原PAA47的纯化和鉴定

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上海免疫学杂志 2003年第1期23卷 32-35页

作者：夏晴仲人前侯晓菁薛菖陈军孔宪涛第二军医大学附属长征医院全军临床免疫中心上海200003

以往的研究发现 6 3 6 4 %的胰腺癌患者具有针对相对分子质量初步鉴定为 4 70 0 0的靶抗原的阳性共带 ,该条带仅在1 9%的正常人中查及 ,本研究旨在纯化并鉴定该靶抗原 ,以深入认识该独特的自身免疫现象在胰腺癌中的意义 ,探索靶抗原的... 详细信息

以往的研究发现 6 3 6 4 %的胰腺癌患者具有针对相对分子质量初步鉴定为 4 70 0 0的靶抗原的阳性共带 ,该条带仅在1 9%的正常人中查及 ,本研究旨在纯化并鉴定该靶抗原 ,以深入认识该独特的自身免疫现象在胰腺癌中的意义 ,探索靶抗原的生物学功能以及在临床诊断中可能应用价值。提取人喉癌上皮细胞 (Hep 2 )蛋白抗原 ,进行双向电泳 ,选取 8份以往在单向蛋白质印迹 (SDS PAGE )中 ,相对分子质量约为 4 70 0 0处有明显抗原抗体反应的胰腺癌患者血清进行免疫印迹分析。比较双向电泳凝胶图像、丽春红染色之蛋白质转印硝酸纤维素滤膜图像以及血清反应后滤膜图像 ,确定靶抗原在凝胶上的斑点 ,判断其等电点。切取胶上蛋白质斑点经胰蛋白酶消化后通过HPLC MS联用分析获得串级质谱数据 ;利用互联网搜索工具MASCOT分析处理数据并进行数据库查询 ,以鉴定靶抗原。结果表明 ,KIAA0 111的基因预测产物是一种新鉴定的胰腺癌相关性自身抗体靶抗原。

关键词：胰腺癌自身抗体免疫印迹法双向电泳

Spraying Arginine at 5 Days before Harvest Delays Postharvest Broccoli Senescence via Nutrient Accumulation

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食品科学 2025年第1期46卷 131-141页

作者： SUN Yupeng chen Ying SHANG Qingwen GUO Yanyin ZHANG Yuxiao WANG Yunqiao xue Qingyue College of Agricultural Engineering and Food Science Shandong University of TechnologyZibo 255049China Zichuan District Agricultural and Rural Affairs Service Center Zibo 255049China

To determine the effects of preharvest arginine spraying on the nutritional level of broccoli and the mechanism of action of arginine in improving the storage quality of broccoli,arginine spraying(5 mmol/L)was conducted at 0,1,3,and 5 days before *** appearance,respiration rate,mass-loss rate,electrolyte leakage,glucosinolate,ascorbic acid,total phenol,total flavonoid,total sugar and sucrose contents,and sucrose phosphate synthase(SPS),invertase(INV),sucrose synthase synthesis(SSS)and cleavage(SSC)activities of broccoli samples were observed after 0,2,4,6,8,and 10 days of *** results showed that spraying arginine at 5 days preharvest(5-ARG)helped to inhibit broccoli respiration during storage,delay electrolyte leakage,and maintain broccoli ***,during the growth stage,total sugar accumulation was higher in the 5-ARG *** addition,during the storage period,sucrose synthesis was accelerated,while sucrose cleavage was inhibited,resulting in more sucrose retention in postharvest *** conclusion,5-ARG resulted in the accumulation of more nutrients during the growth process and effectively delayed the quality decline during storage,thereby prolonging the shelf life of ***,this study provides a theoretical basis for improving postharvest storage characteristics of broccoli through preharvest treatments.

关键词： arginine broccoli preharvest spraying nutrient accumulation storage quality postharvest senescence

Einstein Probe discovery of EP240408a: A peculiar X-ray transient with an intermediate timescale

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 222-240页

作者： Wenda Zhang Weimin Yuan Zhixing Ling Yong chen Nanda Rea Arne Rau Zhiming Cai Huaqing cheng Francesco Coti Zelati Lixin Dai Jingwei Hu Shumei Jia Chichuan Jin Dongyue Li Paul O’Brien Rongfeng Shen Xinwen Shu Shengli Sun Xiaojin Sun Xiaofeng Wang Lei Yang Bing Zhang chen Zhang Shuang-Nan Zhang Yonghe Zhang Jie An David Buckley Alexis Coleiro Bertrand Cordier Liming Dou Rob Eyles-Ferris Zhou Fan Hua Feng Shaoyu Fu Johan P.U.Fynbo Lluis Galbany Saurabh W.Jha Shuaiqing Jiang Albert Kong Erik Kuulkers Weihua Lei Wenxiong Li Bifang Liu Mingjun Liu Xing Liu Yuan Liu Zhu Liu Chandreyee Maitra Alessio Marino Itumeleng Monageng Kirpal Nandra Jeremy Sanders Roberto Soria Lian Tao junfeng Wang Song Wang Tinggui Wang Zhongxiang Wang Qingwen Wu xuefeng Wu Dong Xu Yanjun Xu Suijian xue Yongquan xue Zijian Zhang Zipei Zhu Hu Zou Congying Bao Fansheng chen Houlei chen Tianxiang chen Wei chen Yehai chen Yifan chen chenzhou Cui Weiwei Cui Yanfeng Dai Dongwei Fan Ju Guan Dawei Han Dongjie Hou Haibo Hu Maohai Huang Jia Huo Zhenqing Jia Bowen Jiang Ge Jin chengkui Li junfei Li Longhui Li Maoshun Li Wei Li Zhengda Li Tianying Lian Congzhan Liu Heyang Liu Huaqiu Liu Fangjun Lu Laidan Luo Jia Ma Xuan Mao Haiwu Pan Xin Pan Liming Song Hui Sun Yunyin Tan Qingjun Tang Yihan Tao Hao Wang Juan Wang Lei Wang Wenxin Wang Yilong Wang Yusa Wang Qinyu Wu Haitao Xu Jingjing Xu Xinpeng Xu Yunfei Xu Zhao Xu Changbin xue Yulong xue Ailiang Yan Haonan Yang Xiongtao Yang Yanji Yang Juan Zhang Mo Zhang Wenjie Zhang Zhen Zhang Zhen Zhang Ziliang Zhang Donghua Zhao Haisheng Zhao Xiaofan Zhao Zijian Zhao Hongyan Zhou Yilin Zhou Yuxuan Zhu Zhencai Zhu National Astronomical Observatories Chinese Academy of Sciences School of Astronomy and Space Sciences University of Chinese Academy of Sciences Key Laboratory of Particle Astrophysics Institute of High Energy Physics Chinese Academy of Sciences Institute of Space Sciences (ICE) CSIC Institut d’Estudis Espacials de Catalunya (IEEC) Max Planck Institute for Extraterrestrial Physics Innovation Academy for Microsatellites Chinese Academy of Sciences Department of Physics University of Hong Kong School of Physics and Astronomy University of Leicester School of Physics and Astronomy Sun Yat-sen University Department of Physics Anhui Normal University Shanghai Institute of Technical Physics Chinese Academy of Sciences Physics Department Tsinghua University Nevada Center for Astrophysics University of Nevada Las Vegas Department of Physics and Astronomy University of Nevada Las Vegas South African Astronomical Observatory Department of Astronomy University of Cape Town Department of Physics University of the Free State Université Paris Cité CNRS Astroparticule et Cosmologie Université Paris Cité CEA Paris-Saclay IRFU/DAp-AIM Department of Astronomy Guangzhou University Cosmic Dawn Center (DAWN) Niels Bohr Institute University of Copenhagen Department of Physics and Astronomy Rutgers the State University of New Jersey Institute for Cosmic Ray Research The University of Tokyo ESA/ESTEC Department of Astronomy School of Physics Huazhong University of Science and Technology INAF-Osservatorio Astrofisico di Torino Sydney Institute for Astronomy School of Physics The University of Sydney Department of Astronomy Xiamen University Institute for Frontiers in Astronomy and Astrophysics Beijing Normal University Department of Astronomy University of Science and Technology School of Astronomy and Space Sciences University of Science and Technology of China Institute of Deep Space Sciences Deep Space Exploration Laboratory Department of Astronomy School of Physics and Astronomy Yunnan University Shanghai Astronomical Observatory Chi

We report the discovery of a peculiar X-ray transient,EP240408a,by Einstein Probe （EP） and follow-up studies made with EP,Swift,NICER,GROND,ATCA and other ground-based multiwavelength *** new transient was first detected with Wide-field X-ray Telescope （WXT） on board EP on April 8th,2024,manifested in an intense yet brief X-ray flare lasting for *** flare reached a peak flux of 3.9×10-9erg cm-2s-1in 0.5-4 ke V,～300 times brighter than the underlying X-ray emission detected throughout the *** and more precise follow-up observations by EP/FXT,Swift and NICER confirmed the finding of this new *** X-ray spectrum is non-thermal in 0.5-10 ke V,with a power-law photon index varying *** X-ray light curve shows a plateau lasting for ～4 d,followed by a steep decay till becoming undetectable ～10 d after the initial *** on its temporal property and constraints from previous EP observations,an unusual timescale in the range of 7-23 d is found for EP240408a,which is intermediate between the commonly found fast and long-term *** counterparts have been found in optical and near-infrared,with the earliest observation at 17 h after the initial X-ray detection,suggestive of intrinsically weak emission in these *** demonstrate that the remarkable properties of EP240408a are inconsistent with any of the transient types known so far,by comparison with,in particular,jetted tidal disruption events,gamma-ray bursts,X-ray binaries and fast blue optical *** nature of EP240408a thus remains an *** suggest that EP240408a may represent a new type of transients with intermediate timescales of the order of～10 *** detection and follow-ups of more of such objects are essential for revealing their origin.

关键词： X-ray transients ${\bm~{Einstein~~Probe}}$

Identification of a dual functional betulinic acid analog for the treatment of osteoarthritis by phenotypic screening

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Science China Life Sciences 2025年第1期 300-302页

作者： Ze-Min Lin Mai Xiang Wen-Hui Wei Shu-Hui Fan Li chen Jie Wang Xiao-Qian Yang Chun-Hao Yang Shi-jun He Jian-Ping Zuo Laboratory of Immunopharmacology State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of Sciences School of Pharmacy University of Chinese Academy of Sciences School of Pharmacy Nanjing University of Chinese Medicine State Key Laboratory of Drug Research Department of Medicinal ChemistryShanghai Institute of Materia MedicaChinese Academy of Sciences Innovation Research Institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine

Dear Editor, Phenotypic drug discovery, which observes compound effects on physiology,has given way to targeting specific molecules, but has revived by using modern methods to find drugs based on therapeutic outcomes in disease models(Vincent et al., 2022). Osteoarthritis(OA) is a prevalent chronic joint disorder, primarily affecting knee joints, with osteoclast activation due to low-grade inflammation resulting in increased bone resorption and structural changes in the joint(Katz et al.,2021).

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Stability of Two-Component Bose-Einstein Condensates in Bessel Optical Lattices

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Communications in Theoretical Physics 2008年第6期49卷 1519-1526页

作者： chen Hai-jun LI Hao-Cai xue Ju-Kui College of Physics and Electronics Engineering Northwest Normal University Lanzhou 730070 China

The stability of the ground state of two-component Bose-Einstein condensates （BEGs） loaded into the central well of an axially symmetric Bessel lattices （BLs） potential with attractive or repulsive atoms interactions is studied using the time-dependent Gross-Pitaevskii equation （GPE）. By using the variational method, we find that stable ground state of two-component BEGs can exist in BLs. The BLs＇s depth and the intra-species atom interaction play an important role in the stability of ground state. The collapse of two-component BEGs in BLs is also studied and a collapse condition for trapped two-component BEGs is obtained. It is shown that the two-component BEGs exhibit rich collapse dynamics. That is, the two-component BEGs can collapse in the system with both intra- and inter-attractive, or with intra-attractive and inter-repulsive, or with intra-repulsive and inter-attractive atom interactions. Furthermore, the control of the collapse of the two-component BEGs in BLs is discussed in detail. The stability diagram of the ground state in parameter space is obtained. The results show that the collapse of two-component BEGs can be controlled by temporal modulation of the atom interaction.

关键词： two-component Bose-Einstein condensates Gross-Pitaevskii equation Bessel optical lattice ground state

Reprogrammed CRISPR-Cas9 targeting the conserved regions of HPV6/11 E7 genes inhibits proliferation and induces apoptosis in E7-transformed keratinocytes

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Asian Journal of Andrology 2016年第3期18卷 475-479,I0011,I0012页

作者： Yu-chen Liu Zhi-Ming Cai xue-jun Zhang Institute of Dermatology Department of Dermatology The First Affiliated Hospital Anhui Medical University Hefei China Key Laboratory of Dermatology Ministry of Education State Key-Laboratory of Dermatology Incubation Center Department of Dermatology Anhui Medical University Hefei China Key Laboratory of Medical Reprogramming Technology Department of Urology The Genitourinary Institution of Shenzhen University Shenzhen Second People's Hospital The First Affiliated Hospitalof Shenzhen University Shenzhen China.

The persistence infection of low-risk type （type 6 or type 11） of human papillomavirus （HPV） is the main cause of genital warts. Given the high rate of recurrence after treatment, the use of a new molecular agent is certain to be of value. The aim of this study was to achieve targeted inactivation of viral E7 gene in keratinocytes using the reprogrammed clustered regularly interspaced short palindromic repeats （CRISPR）/CRISPR-associated （Cas） 9 system. To accomplish this, a universal CRISPR-Cas9 system for targeting both HPV6/11 E7 genes was constructed by using a dual guide RNA vector. After transfection of the vector into E7-transfromed keratinocytes, the expression level of E7 protein was measured using western-blot analysis and the sequence of the E7 gene was determined using Sanger sequencing. Cell proliferation was analyzed by CCK-8 assay, and cell apoptosis was evaluated by Hoechst 33258 staining, flow cytometry analysis and ELISA assay. The results indicated that both HPV6/11 E7 genes can be inactivated by the single CRISPR-Cas9 system. Furthermore, silencing of E7 led to inhibition of cell proliferation and induction of apoptosis in E7-transfromed keratinocytes but not in normal keratinocytes. Our data suggested that the reprogrammed CRISPR-Cas9 system has the potential for the development of an adjuvant therapy for genital warts.

关键词： apoptosis CRISPR-Cas9 HPV E7 proliferation

Aerodynamic modeling and stability analysis of a high-speed train under strong rain and crosswind conditions

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第12期12卷 964-970页

作者： xue-ming SHAO jun WAN Da-wei chen Hong-bing XIONG Department of Mechanics Zhejiang University Hangzhou 310027 China National Engineering Laborat ory for System Integration of High-Speed Train （South） CSR Qingdao Sifang Co. Ltd. Qingdao 266111 China

With the development of high-speed train,it is considerably concerned about the aerodynamic characteristics and operation safety issues of the high-speed train under extreme weather *** aerodynamic performance of a high-speed train under heavy rain and strong crosswind conditions are modeled using the Eulerian two-phase model in this *** impact of heavy rainfall on train aerodynamics is investigated,coupling heavy rain and a strong *** show that the lift force,side force,and rolling moment of the train increase significantly with wind speed up to 40 m/s under a rainfall rate of 60 mm/*** considering the rain and wind *** increases of the lift force,side force,and rolling moment may deteriorate the train operating safety and cause the train to overturn.A quasi-static stability analysis based on the moment balance is used to determine the limit safety speed of a train under different rain and wind *** results can provide a frame of reference for the train safe operation under strong rain and crosswind conditions.

关键词： High-speed train Aerodynamic characteristics Multiphase flow Rain Crosswind Overturning

Application of grey relational analysis in sheet metal forming for multi-response quality characteristics

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第5期8卷 805-811页

作者： XIE Yan-min YU Hu-ping chen jun RUAN xue-yu National Die ＆ Mold CAD Engineering Research Center Shanghai Jiao Tong University Shanghai 200030 China

The theory of grey systems is a new technique for performing prediction, relational analysis and decision making in many areas. In this paper, the use of grey relational analysis for optimizing the square hole flanging process parameters with considerations of the multiple response (the average flanging height, regular flanging and maximum strain) is introduced. Various flanging parameters, such as the blank inner radius rb, blank inner width B0, are considered. An orthogonal array is used for the experimental design. Multiple response values are obtained using finite element analysis (FEA). Optimal process parameters are determined by the grey relational grade obtained from the grey relational analysis for multi-performance characteristics (flanging height, regular flanging and maximum strain). Analysis of variance (ANOVA) for the grey relational grade is implemented. The results showed good agreement with the experiment result. Grey relational analysis can be applied in multiple response optimi-zation designs.

关键词： Grey relational analysis Flanging Multiple response Optimization design

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某塑料制品公司职业病危害预评价分析

实用预防医学 2010年第7期17卷 1316-1317页

作者：黎雪军陈朔陈泽香谢松玲黄够多湖南省长沙市雨花区卫生监督所湖南长沙410014

目的对某塑料制品公司新建生产线进行评价分析,为预防职业病危害提供科学依据。方法按照《建设项目职业病危害预评价技术导则》GBZ/T196-2007[1]的要求进行评价,选择该企业老生产线进行类比调查。结果该项目属于一般职业病危害建设项目... 详细信息

目的对某塑料制品公司新建生产线进行评价分析,为预防职业病危害提供科学依据。方法按照《建设项目职业病危害预评价技术导则》GBZ/T196-2007[1]的要求进行评价,选择该企业老生产线进行类比调查。结果该项目属于一般职业病危害建设项目,可能产生职业病危害因素主要为噪声和粉尘。结论该建设项目选址与总体布局、生产设备布局合理,拟采取的职业病防护措施设计均符合国家卫生标准,职业卫生评价合格。

关键词：塑料制品职业病危害预评价