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癌症 2009年第8期28卷 856-860页

作者：陈学军杨建华郑伟浙江大学医学院附属第二医院妇科浙江杭州310009

背景与目的:水通道蛋白5(aquaporin 5,AQP5)的表达异常与卵巢癌细胞的浸润转移和血管形成有关。核转录因子NF-κB参与细胞的增殖、分化、凋亡,且能影响肿瘤细胞对化疗的敏感性。本研究探讨拓扑替康(topotecan,TPT)对卵巢癌SKOV3细胞中A... 详细信息

背景与目的:水通道蛋白5(aquaporin 5,AQP5)的表达异常与卵巢癌细胞的浸润转移和血管形成有关。核转录因子NF-κB参与细胞的增殖、分化、凋亡,且能影响肿瘤细胞对化疗的敏感性。本研究探讨拓扑替康(topotecan,TPT)对卵巢癌SKOV3细胞中AQP5、核转录因子(nuclear factor kappaB,NF-κB)及其抑制因子(inhibitor kappa B,IκBα)表达的影响。方法:用不同浓度TPT作用SKOV3细胞不同时间后,应用MTT法检测并计算卵巢癌细胞增殖抑制率,蛋白质印迹法检测AQP5、NF-κB及IκBα蛋白表达,并分析各蛋白表达水平的相关性。结果:0.4μg/mLTPT与SKOV3细胞温育24h,AQP5、胞浆和胞核NF-κBp65以及胞浆IκBα表达随着作用时间延长均明显下降,并维持至72h后(P<0.05)。不同浓度(0.2μg/mL、0.4μg/mL、0.6μg/mL、0.8μg/mL)TPT作用于SKOV3细胞24h,随着TPT浓度增加,SKOV3细胞AQP5表达量逐渐减少(P<0.05),0.8μg/mLTPTAQP5表达抑制率为57.9%;同时,细胞核NF-κBp65、IκBα表达量也逐渐减少。TPT对细胞的增殖抑制率与AQP5表达呈负相关(r=-0.965;P<0.05),AQP5表达与NF-κBp65和IκBα呈正相关(r=0.903,0.896;P<0.05)。结论:TPT抑制肿瘤细胞增殖时下调AQP5及核转录因子NF-κB表达。

关键词：卵巢肿瘤水通道蛋白5 NF-κB 拓扑替康

Dynamic Recrystallization Behavior and Microstructural Evolution in SPHC Steel

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Journal of Shanghai Jiaotong university(Science) 2010年第3期15卷 301-306页

作者：伍来智李雪松陈军张鸿冰崔振山 Department of Plasticity Technology Shanghai Jiaotong University

The dynamic recrystallization(DRX) behaviors in SPHC steel were investigated with hot compression tests at deformation temperatures of 950-1 150,strain rates of 0.1-15 s-1,and initial austenite grain sizes of 86-232 μ*** effects of deformation temperature,strain,strain rate and the initial austenite grain size on the microstructural evolution during DRX were studied in *** results show that DRX is observed under the condition of the Zener-Hollomon parameter being less than 1.07×10 13 *** deformation activation energy for SPHC steel is calculated to be 299.4 kJ/mol by regression *** grain size of DRX is refined with decreasing temperature and increasing strain rate under steady state conditions,but it is not influenced by the initial grain *** mathematical equation of DRX grain size of SPHC steel is obtained.

关键词： SPHC steel dynamic recrystallization(DRX) austenite grain size grain refinement

Mechanistic insight into the synergy between nickel single atoms and nanoparticles on N-doped carbon for electroreduction of CO2

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Journal of Energy Chemistry 2025年第1期100卷 327-336页

作者： Mingdong Sun Wenwen Guan Cailing chen Chao Wu Xiaoling Liu Biao Meng Tao chen Yu Han jun Wang Shibo Xi Yu Zhou State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical Engineering Nanjing Tech University Advanced Membranes and Porous Materials Center Physical Sciences and Engineering Division King Abdullah University of Science and Technology Institute of Sustainability for Chemicals Energy and Environment A*STAR (Agency for Science Technology and Research) College of Materials Science and Engineering Sichuan University

The synergy of single atoms （SAs） and nanoparticles （NPs） has demonstrated great potential in promoting the electrocatalytic carbon dioxide reduction reaction （CO2RR）;however,the rationalization of the SAs/NPs proportion remains one challenge for the catalyst ***,a Ni2+-loaded porous poly（ionic liquids）(PIL) precursor synthesized through the free radical self-polymerization of the ionic liquid monomer,1-allyl-3-vinylimidazolium chloride,was pyrolyzed to prepare the Ni,N co-doped carbon materials,in which the proportion of Ni SAs and NPs could be facilely modulated by controlling the annealing *** catalyst Ni-NC-1000 with a moderate proportion of Ni SAs and NPs exhibited high efficiency in the electrocatalytic conversion of CO2into *** Ni K-edge X-ray absorption near-edge structure （XANES） spectra and theoretical calculations were conducted to gain insight into the synergy of Ni SAs and *** charge transfer from Ni NPs to the surrounding carbon layer and then to the Ni SAs resulted in the electron-enriched Ni SAs active *** the electroreduction of CO2,the coexistence of Ni SAs and NPs strengthened the CO2activation and the affinity towards the key intermediate of*COOH,lowering the free energy for the potential-determining*CO2→*COOH step,and therefore promoted the catalysis efficiency.

关键词： Single atom catalyst Synergy effect Carbon catalyst Electrocatalysis

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细菌纤维素制备及吸附金属离子的研究进展

精细化工 2018年第5期35卷 721-727页

作者：李治明荣荣尹学琼陈俊华朱莉海南大学海南省精细化工工程技术研究中心

细菌纤维素（BC）是一种由微生物发酵技术制备的纤维素,分子内存在大量的羟基,具有超细网状纳米纤维结构,比表面积高,其独特的结构和物理化学性质对溶液中金属离子吸附性能起着重要作用。为了加快BC的发展及其对重金属吸附的应用,首先... 详细信息

细菌纤维素（BC）是一种由微生物发酵技术制备的纤维素,分子内存在大量的羟基,具有超细网状纳米纤维结构,比表面积高,其独特的结构和物理化学性质对溶液中金属离子吸附性能起着重要作用。为了加快BC的发展及其对重金属吸附的应用,首先介绍了影响细菌纤维素的制备因素,例如：菌种、常用碳源及添加助剂。其次重点介绍了BC对Cu^2＋、Pb^2＋、Cd^2＋、Cr^6＋及其他金属离子（如Pd^2＋、Ag^＋、Au^3＋、Fe^2＋、Sb^3＋）等的吸附性能。然后对BC吸附金属离子机理进行了综述。最后,对BC的应用前景进行了展望。

关键词：细菌纤维素碳源金属离子吸附机理

Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97

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World Journal of Gastroenterology 2001年第5期7卷 630-636页

作者： Yan Li Zhao-You Tang Sheng-Long Ye Yin-Kun Liu Jie chen Qiong xue jun chen Dong-Mei Gao Wei-Hua Bao Liver Cancer Institute and Zhongshan Hospital of Fudan University (Former Liver Cancer Institute of Shanghai Medical University),Shanghai 200032,China Liver Cancer Institute & Zhongshan Hospital of Fudan University 136 Yixueyuan Road Shanghai 200032China.

AIM: To establish clone cells with different metastatic potential for the study of metastasis-related mechanisms. METHODS: Cloning procedure was performed on parental hepatocellular carcinoma (HCC) cell line MHCC97, and biological characteristics of the target clones selected by in vivo screening were studied. RESULTS: Two clones with high (MHCC97-H) and low (MHCC97-L) metastatic potential were isolated from the parent cell line. Compared with MHCC97-L, MHCC97-H had smaller cell size (average cell diameter 43 microm vs 50 microm) and faster in vitro and in vivo growth rate (tumor cell doubling time was 34.2h vs 60.0h). The main ranges of chromosomes were 55-58 in MHCC97-H and 57-62 in MHCC97-L. Boyden chamber in vitro invasion assay demonstrated that the number of penetrating cells through the artificial basement membrane was (37.5 +/- 11.0) cells/field for MHCC97-H vs (17.7 +/- 6.3)/field for MHCC97-L. The proportions of cells in G0-G1 phase, S phase, and G2-M phase for MHCC97-H/MHCC97-L were 0.56/0.65, 0.28/0.25 and 0.16/0.10, respectively, as measured by flow cytometry. The serum AFP levels in nude mice 5wk after orthotopic implantation of tumor tissue were (246 +/- 66) microg.L(-1) for MHCC97-H and (91 +/- 66) microg.L(-1) for MHCC97-L. The pulmonary metastatic rate was 100% (10/10) vs 40% (4/10). CONCLUSION: Two clones of the same genetic background but with different biological behaviors were established, which could be valuable models for investigation on HCC metastasis.

关键词： Albumins Animals Carcinoma, Hepatocellular Cell Division Chromosomes Clone Cells Flow Cytometry Hepatitis B Hepatitis B Surface Antigens Hepatitis B virus purification Humans Keratin Liver Liver Neoplasms, Experimental Male Mice Mice, Inbred BALB C Mice, Nude Neoplasm Invasiveness Research Support, Non-U.S. Gov't Tumor Cells, Cultured Virus Integration alpha-Fetoproteins

Construction of 3D porous Cu_(1.81)S/nitrogen-doped carbon frameworks for ultrafast and long-cycle life sodium-ion storage

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 191-200页

作者： chen chen Hongyu xue Qilin Hu Mengfan Wang Pan Shang Ziyan Liu Tao Peng Deyang Zhang Yongsong Luo Key Laboratory of Microelectronics and Energy of Henan Province Henan Joint International Research Laboratory of New Energy Storage TechnologyCollege of Physics and Electronic EngineeringXinyang Normal UniversityXinyang 464000China School of Physics and Electronic Engineering Nanyang Normal UniversityNanyang 473061China

Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific ***,the inferior intrinsic conductivity and large volume variation during sodiation-desodiation processes seriously affect its high-rate and long-cyde performance,unbeneficial for the application as fast-charging and long-cycling SIBs ***,the three-dimensional porous Cu_(1.81)S/nitrogen-doped carbon frameworks(Cu_(1.81)S/NC)are synthesized by the simple and facile sol-gel and annealing processes,which can accommodate the volumetric expansion of Cu_(1.81)S nanoparticles and accelerate the transmission of ions and electrons during Na^(+)insertion/extraction processes,exhibiting the excellent rate capability(250.6 mA·g^(-1)at 20.0 A·g^(-1))and outstanding cycling stability(70% capacity retention for 6000 cycles at 10.0 A·g^(-1))for ***,the Na-ion full cells coupled with Na_(3)V_(2)(PO_(4))_(3)/C cathode also demonstrate the satisfactory reversible specific capacity of 330.5 mAh·g^(-1)at 5.0 A·g^(-1)and long-cycle performance with the 86.9% capacity retention at 2.0 A·g^(-1)after 750 *** work proposes a promising way for the conversionbased metal sulfides for the applications as fast-charging sodium-ion battery anode.

关键词： copper sulfide nanoparticles porous carbon framework fast charging long-cycle performance sodium-ion full batteries

Mesospheric tide comparisons at low latitudes observed by two collocated meteor radars

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Earth and Planetary Physics 2025年第1期9卷 54-68页

作者： Jian Li Wen Yi XiangHui xue Jie Zeng HaiLun Ye JianYuan Wang JinSong chen Na Li TingDi chen Chinese Academy of Sciences(CAS)Key Laboratory of Geospace Environment Department of Geophysics and Planetary SciencesUniversity of Science and Technology of ChinaHefei 230026China CAS Center for Excellence in Comparative Planetology Anhui Mengcheng Geophysics National Observation and Research StationUniversity of Science and Technology of ChinaHefei 230026China Hefei National Laboratory University of Science and Technology of ChinaHefei 230026China Collaborative Innovation Center of Astronautical Science and Technology Harbin 150001China National Key Laboratory of Electromagnetic Environment China Research Institute of Radiowave PropagationQingdao 266107China Yunnan Kunming Electromagnetic Environment National Observation and Research Station Qujing Kunming 655000China

Accurate knowledge of mesospheric winds and waves is essential for studying the dynamics and climate in the mesosphere and lower thermosphere(MLT)*** this study,we conduct a comparative analysis of the mesosphere tidal results obtained from two adjacent meteor radars at low latitudes in Kunming,China,from November 2013 to December *** two radars operate at different frequencies of 37.5 MHz and 53.1 MHz,***,overall good agreement is observed between the two radars in terms of horizontal winds and tide *** results show that the dominant tidal waves of the zonal and meridional winds are diurnal and semidiurnal ***,we conduct an exhaustive statistical analysis to compare the tidal amplitudes and vertical wavelengths recorded by the dual radar systems,which reveals a high degree of alignment in tidal *** investigation includes variances and covariances of tidal amplitudes,which demonstrate remarkable consistency across measurements from both *** finding highlights clear uniformity in the mesospheric tidal patterns observed at low latitudes by the two neighboring meteor *** of the comparative analysis specifically underscore the significant correlation in vertical wavelength measurements,validating the robustness of radar observations for tidal research.

关键词： mesosphere and lower thermosphere region meteor radar mesospheric winds tides

一种嵌入项目元数据的跨项目协同过滤推荐算法

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小型微型计算机系统 2018年第4期39卷 707-712页

作者：王秀刘学军陈振春邵帅南京工业大学计算机科学与技术学院南京211816

传统的协同过滤推荐系统中用户相似性通常是由余弦、Pearson等方法来计算.然而这些方法都是基于共同的评分项目,在评分数据稀疏的情况下,用户之间的共同评分项目较少,很难准确地找到与目标用户偏好相似的邻居.针对这一问题,提出一种新... 详细信息

传统的协同过滤推荐系统中用户相似性通常是由余弦、Pearson等方法来计算.然而这些方法都是基于共同的评分项目,在评分数据稀疏的情况下,用户之间的共同评分项目较少,很难准确地找到与目标用户偏好相似的邻居.针对这一问题,提出一种新的方法,通过嵌入项目元数据,利用用户与项目及其属性的交互,采用贝叶斯概率计算项目特征的相似性,并结合EM D方法实现基于元数据表示的跨项目计算相似性.实验结果表明,该方法能够有效地解决用户评分数据稀疏情况下传统的相似性度量方法所存在的不足,提高了推荐的准确性.

关键词：协同过滤数据稀疏项目元数据跨项目

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基于方差不变特性的快速分形图像编码方法

通信学报 2002年第2期23卷 88-91页

作者：王学军陈贺新吉林大学通信工程学院吉林长春130012

本文结合图像子块的自身统计特性,提出一种基于方差不变特性的快速分形编码方法。实验结果表明,该方法可使编码速度有较大提高,而解码图像质量无明显下降;且有相对确定的编码时间,有助于编码系统的硬件实现。

关键词：图像编码方差不变特性快速分形