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The Main Biological Hazards in Animal Biosafety Level 2 Facilities and Strategies for Control

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Biomedical and Environmental Sciences 2016年第4期29卷 300-304页

作者： LI Xiao Yan xue kang ning JIANG Jin Sheng LU Xuan Cheng Laboratory Animal Center Chinese Center for Disease Control and Prevention Infrastructure Construction Department Chinese Center for Disease Control and Prevention

Concern about the biological hazards involved in microbiological research, especially research involving laboratory animals, has increased in recent years. Working in an animal biosafety level 2 facility （ABSL-2）, commonly used for research on infectious diseases, poses various biological hazards. Here, the regulations and standards related to laboratory biosafety in China are introduced, the potential biological hazards present in ABSL-2 facilities are analyzed, and a series of strategies to control the hazards are presented.

关键词： The Main Biological Hazards in Animal Biosafety Level 2 Facilities and Strategies for Control

Enhancing microbial superoxide generation and conversion to hydroxyl radicals for enhanced bioremediation using iron-binding ligands

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Journal of Environmental Sciences 2025年第1期147卷 597-606页

作者： Yuhan Wang xue ning Jinsong Liang Aijie Wang Jiuhui Qu School of Civil and Environmental Engineering Harbin Institute of TechnologyShenzhen 518055China SFI MaREI Centre for Energy Climate and MarineEnvironmental Research InstituteUniversity College CorkCorkIreland Center for Water and Ecology Tsinghua UniversityBeijing 100084China

Harnessing bacteria for superoxide production in bioremediation holds immense promise,yet its practical application is hindered by slow production rates and the relatively weak redox potential of *** study delves into a cost-effective approach to amplify superoxide production using an Arthrobacter strain,a prevalent soil bacterial *** research reveals that introducing a carbon source along with specific iron-binding ligands,including deferoxamine(DFO),diethylenetriamine pentaacetate(DTPA),citrate,and oxalate,robustly augments microbial superoxide ***,our findings suggest that these iron-binding ligands play a pivotal role in converting superoxide into hydroxyl radicals by modulating the electron transfer rate between Fe(Ⅲ)/Fe(Ⅱ)and ***,among the tested ligands,only DTPA emerges as a potent promoter of this conversion process when complexed with Fe(Ⅲ).We identify an optimal Fe(Ⅲ)to DTPA ratio of approximately 1:1 for enhancing hydroxyl radical production within the Arthrobacter *** research underscores the efficacy of simultaneously introducing carbon sources and DTPA in facilitating superoxide production and its subsequent conversion to hydroxyl radicals,significantly elevating bioremediation ***,our study reveals that DTPA augments superoxide production in cultures of diverse soils,with various soil microorganisms beyond Arthrobacter identified as contributors to superoxide *** emphasizes the universal applicability of DTPA across multiple bacterial *** conclusion,our study introduces a promising methodology for enhancing microbial superoxide production and its conversion into hydroxyl *** findings hold substantial implications for the deployment of microbial reactive oxygen species in bioremediation,offering innovative solutions for addressing environmental contamination challenges.

关键词： Superoxide Hydroxyl radical Iron-binding ligand Bioremediation DTPA

Integration of wearable electronics and heart rate variability for human physical and mental well-being assessment

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Journal of Semiconductors 2025年第1期46卷 58-76页

作者： Feifei Yin Jian Chen Haiying xue Kai kang Can Lu Xinyi Chen Yang Li East China Institute of Photo-Elextro ICs Suzhou 215163China Bengbu Representative Department Bengbu 233000China School of Integrated Circuits Shandong UniversityJinan 250101China

Heart rate variability(HRV)that can reflect the dynamic balance between the sympathetic nervous and parasympathetic nervous of human autonomic nervous system(ANS)has attracted considerable ***,traditional electrocardiogram(ECG)devices for HRV analysis are bulky,and hard wires are needed to attach measuring electrodes to the chest,resulting in the poor wearable experience during the long-term *** with that,wearable electronics enabling continuously cardiac signals monitoring and HRV assessment provide a desirable and promising approach for helping subjects determine sleeping issues,cardiovascular diseases,or other threats to physical and mental *** now,significant progress and advances have been achieved in wearable electronics for HRV monitoring and applications for predicting human physical and mental *** this review,the latest progress in the integration of wearable electronics and HRV analysis as well as practical applications in assessment of human physical and mental health are *** commonly used methods and physiological signals for HRV analysis are briefly ***,we highlighted the research on wearable electronics concerning HRV assessment and diverse applications such as stress estimation,drowsiness detection,***,the current limitations of the integrated wearable HRV system are concluded,and possible solutions in such a research direction are outlined.

关键词： wearable electronics HRV analysis physical and mental well-being machine learning stress detection

Recent Advancements in the Optimization Capacity Configuration and Coordination Operation Strategy of Wind-Solar Hybrid Storage System

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Energy Engineering 2025年第1期122卷 285-306页

作者： Hongliang Hao Caifeng Wen Feifei xue Hao Qiu ning Yang Yuwen Zhang Chaoyu Wang Edwin E.Nyakilla Department of New Energy Storage Peking University Ordos Research Institute of EnergyOrdos017010China School of Energy and Power Engineering Inner Mongolia University of TechnologyHohhot010080China Key Laboratory of Wind Energy and Solar Energy Utilization Technology Inner Mongolia University of TechnologyHohhot010051China

Present of wind power is sporadically and cannot be utilized as the only fundamental load of energy *** paper proposes a wind-solar hybrid energy storage system(HESS)to ensure a stable supply grid for a longer period.A multi-objective genetic algorithm(MOGA)and state of charge(SOC)region division for the batteries are introduced to solve the objective function and configuration of the system capacity,***/Simulink was used for simulation *** optimization results show that for a 0.5 MW wind power and 0.5 MW photovoltaic system,with a combination of a 300 Ah lithium battery,a 200 Ah lead-acid battery,and a water storage tank,the proposed strategy reduces the system construction cost by approximately 18,000 ***,the cycle count of the electrochemical energy storage systemincreases from4515 to 4660,while the depth of discharge decreases from 55.37%to 53.65%,achieving shallow charging and discharging,thereby extending battery life and reducing grid voltage fluctuations *** proposed strategy is a guide for stabilizing the grid connection of wind and solar power generation,capability allocation,and energy management of energy conservation systems.

关键词： Electric-thermal hybrid storage modal decomposition multi-objective genetic algorithm capacity optimization allocation operation strategy

Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

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Neural Regeneration Research 2025年第7期20卷 2116-2128页

作者： Yuxue Mu ning Zhang Dongyu Wei Guoqing Yang Lilingxuan Yao Xinyue Xu Yang Li Junhui xue Zuoming Zhang Tao Chen Aerospace Clinical Medical Center School of Aerospace MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Aviation Medicine Xijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Emergency Medicine Wuhan No.1 HospitalWuhanHubei ProvinceChina Third Regiment School of Basic MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina Fourth Regiment School of Basic MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina

A microgravity environment has been shown to cause ocular damage and affect visual acuity,but the underlying mechanisms remain ***,we established an animal model of weightlessness via tail suspension to examine the pathological changes and molecular mechanisms of retinal damage under *** 4 weeks of tail suspension,there were no notable alterations in retinal function and morphology,while after 8 weeks of tail suspension,significant reductions in retinal function were observed,and the outer nuclear layer was thinner,with abundant apoptotic *** investigate the mechanism underlying the degenerative changes that occurred in the outer nuclear layer of the retina,proteomics was used to analyze differentially expressed proteins in rat retinas after 8 weeks of tail *** results showed that the expression levels of fibroblast growth factor 2(also known as basic fibroblast growth factor)and glial fibrillary acidic protein,which are closely related to Müller cell activation,were significantly *** addition,Müller cell regeneration and Müller cell gliosis were observed after 4 and 8 weeks,respectively,of simulated *** findings indicate that Müller cells play an important regulatory role in retinal outer nuclear layer degeneration during weightlessness.

关键词： glial fibrous acidic protein gliosis Müller cells nerve growth factor neural differentiation neurodegeneration proteomic retinal degeneration retinal outer nuclear layer simulated weightlessness

黄藤素柔性纳米脂质体的制备及体外透黏膜给药研究

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中国药学杂志 2015年第24期50卷 2139-2145页

作者：李伟泽付丽娜赵宁袁琪康雪宁王欢西安医学院药学院西安710021

目的制备黄藤素柔性纳米脂质体(PFNL),并考察其药剂学性质与体外透黏膜给药的行为特点,为进一步应用奠定基础。方法以薄膜分散法制备黄藤素柔性纳米脂质体,采用鱼精蛋白凝聚与高效液相色谱仪测定包封率,考察卵磷脂、胆固醇与丙二醇对脂... 详细信息

目的制备黄藤素柔性纳米脂质体(PFNL),并考察其药剂学性质与体外透黏膜给药的行为特点,为进一步应用奠定基础。方法以薄膜分散法制备黄藤素柔性纳米脂质体,采用鱼精蛋白凝聚与高效液相色谱仪测定包封率,考察卵磷脂、胆固醇与丙二醇对脂质体包封率的影响;用透射电镜(TEM)、光子相关光谱仪(PCS)与激光共聚焦显微镜(CLSM)评价脂质体的粒径和结构;通过测定脂质体透过微孔滤膜的相对速率评价其变形性;用恒温电导率法测定脂质体的凝聚速度常数(K)以评价其物理稳定性。采用水平双室扩散池考察黄藤素柔性纳米脂质体通过猪阴道黏膜的给药行为,并考察其对大鼠阴道组织中细胞因子SLPI、LF和SP-D表达的影响,与黄藤素普通脂质体(PCL)及其溶液剂(PL)进行对比。结果卵磷脂、胆固醇和丙二醇的质量分数分别为3%、0.02%、20%时,制备的黄藤素柔性纳米脂质体对黄藤素的包封率为(78±2.13)%;黄藤素柔性纳米脂质体为圆形或椭圆形的多层状囊泡结构,粒径为(185±19)nm,Zeta电位为(-53±2.27)m V,变形性为(79±5.75)%;黄藤素柔性纳米脂质体K值始终低于黄藤素的普通脂质体的K值。6.0 h时,黄藤素柔性纳米脂质体的药物累积透过量分别为黄藤素的普通脂质体和其溶液剂的1.53与2.86倍,且药物在阴道组织中的滞留量也高于黄藤素的普通脂质体和其溶液剂;黄藤素柔性纳米脂质体不影响大鼠阴道组织中SLPI、LF和SP-D表达,组织切片未显示异常。结论黄藤素柔性纳米脂质体对黄藤素的包封率高、稳定性好,能够促进药物通过阴道黏膜并在黏膜组织中形成药物储库,对阴道安全无刺激,因此,可作为黄藤素阴道给药的一种新型给药载体。

关键词：黄藤素柔性纳米脂质体药剂学性质透黏膜给药

Efficient photodegradation of perfluoroalkyl substances under visible light by hexagonal ZnIn_(2)S_(4) nanosheets

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Journal of Environmental Sciences 2025年第2期148卷 116-125页

作者： Fuyu Liu Hui xue Tingshuo kang Qiupei Lei Jiazhe Chen Zhihong Zuo Bin Han Xize Lu Xiao Yang Xiangcheng Shan Xiaoyang Song Qingzhe Zhang Yongguang Yin Yong Cai Shandong Key Laboratory of Environmental Processes and Health Qingdao Key Laboratory of Marine Pollutant PreventionSchool of Environmental Science and EngineeringShandong UniversityQingdao 266237China Research Center for Particle Science and Technology Institute of Frontier and Interdisciplinary ScienceShandong UniversityQingdao 266237China Shenzhen Research Institute of Shandong University A301 Virtual University Park in South DistrictShenzhen 518057China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 10085China Department of Chemistry and Biochemistry Florida International UniversityMiamiFlorida 33199USA

Perfluoroalkyl substances(PFASs)are typical persistent organic pollutants,and their removal is urgently required but *** has shown potential in PFASs degradation due to the redox capabilities of photoinduced charge carriers in ***,hexagonal ZnIn_(2)S_(4)(ZIS)nanosheets were synthesized by a one-pot oil bath method and were well characterized by a series of *** the degradation of sodium p-perfluorous nonenoxybenzenesulfonate(OBS),one kind of representative PFASs,the assynthesized ZIS showed activity superior to P25 TiO_(2) under both simulated sunlight and visible-light *** good photocatalytic performance was attributed to the enhanced light absorption and facilitated charge *** pH conditions were found crucial in the photocatalytic process by influencing the OBS adsorption on the ZIS *** e−and h+were the main active species involved in OBS degradation in the ZIS *** work confirmed the feasibility and could provide mechanistic insights into the degradation and defluorination of PFASs by visible-light photocatalysis.

关键词： PFASs Defluorination Photocatalysis Visible light ZnIn_(2)S_(4)

The N-terminal domain of gasdermin D induces liver fibrosis by reprogrammed lipid metabolism

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Animal Models and Experimental Medicine 2025年第1期8卷 114-125页

作者： xue Wang Chunyou ning Xingyi Cheng Zhengzhong Wu Dongbo Wu xuemei Ding Cunxiang Ju Zhihang Zhou Lingfeng Wan Wei Zhao Peiliang Shi GemPharmatech Chengdu Co. Ltd.ChengduChina Center of Infectious Diseases West China HospitalSichuan UniversityChengduChina Gempharmatech Shanghai Co. Ltd.ShanghaiChina Department of Gastroenterology the Second Affiliated Hospital of Chongqing Medical UniversityChongqingChina Fatty Liver Disease Center of Integrated Chinese and Western Medicine Affiliated Hospital of Nanjing University of Chinese MedicineNanjingChina School of Clinical Medicine and The First Affiliated Hospital of Chengdu Medical College ChengduChina Department of Clinical Biochemistry School of Laboratory MedicineChengdu Medical CollegeChengduChina GemPharmatech Co. Ltd.GuangdongChina

Background:The emerging incidence of pathogenic liver conditions is turning into a major concern for global *** of pyroptosis in hepatocytes instigates cel-lular disintegration,which in turn liberates substantial quantities of pro-inflammatory intracellular substances,thereby accelerating the advancement of liver ***,directing therapeutic efforts towards inhibiting pyroptosis could po-tentially serve as an innovative approach in managing inflammation related chronic hepatic ***:GSDMD-NT^(ki/wt)mice and Alb-cre^(ki/wt)mice were generated using CRISPR/Cas9 *** crossing the two strains together,we induced conditional cell death by doxycycline to construct a mouse model of liver *** analyzed differ-entially expressed genes by RNA sequencing and explored their biological *** efficacy of obeticholic acid(OCA)in the treatment of liver fibrosis was ***:Doxycycline-treated GSDMD-NT^(ki/wt)×Alb-cre^(ki/wt)mice showed severe liver damage,vacuolation of hepatocytes,increased collagen fibers,and accumulation of lipid *** expression of liver fibrosis related genes was greatly increased in the doxycycline-treated mouse liver compared with untreated mouse ***-sequencing showed that upregulated differentially expressed genes were involved in inflammatory responses,cell activation,and metabolic *** with OCA alleviated the liver fibrosis,with reduced ALT and AST levels seen in the GSDMD-NT^(ki/wt)×Alb-cre^(ki/wt)***:We successfully constructed a novel mouse model for liver *** GSDMD-NT-induced fibrosis may be mediated by abnormal lipid *** re-sults demonstrated that we successfully constructed a mouse model of liver fibrosis,and GSDMD-NT induced fibrosis by mediating lipid metabolism.

关键词： GSDMD-NT lipid metabolism liver fibrosis NASH pyroptosis

Capsaicin(CAP) exerts a protective effect against ethanol-induced oxidative gastric mucosal injury by modulating the chemokine receptor 4 (CCR4)/Src/p47phox signaling pathway both in vitro and in vivo

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Chinese Journal of Natural Medicines 2025年第2期23卷 191-202页

作者： Zhiru Yang Haolin Guo Pengfei Zhang Kairui Liu Junli Ba xue Bai Shiti Shama Bo Zhang Xiaoning Gao Jun kang School of Life Sciences Tianjin University School of Traditional Chinese Medicine Beijing University of Chinese Medicine Tianjin Pharmaceutical and Cosmetic Evaluation and Inspection Center Institute for TCM-X MOE Key Laboratory of Bioinformatics Bioinformatics Division BNRist Department of Automation Tsinghua University Institute of Biomedical Research Henan Academy of Sciences

Ethanol(EtOH) is a common trigger for gastric mucosal diseases, and mitigating oxidative stress is essential for attenuating gastric mucosal damage. Capsaicin(CAP) has been identified as a potential agent to counteract oxidative damage in the gastric mucosa; however, its precise mechanism remains unclear. This study demonstrates that CAP alleviates EtOH-induced gastric mucosal injuries through two primary pathways: by suppressing the chemokine receptor 4(CCR4)/Src/p47phox axis, thereby reducing oxidative stress, and by inhibiting the phosphorylation and nuclear translocation of nuclear factor-κB p65(NF-κB) p65, resulting in diminished inflammatory responses. These findings elucidate the mechanistic pathways of CAP and provide a theoretical foundation for its potential therapeutic application in the treatment of gastric mucosal injuries.

关键词： Capsaicin Chemokine signaling Gastric mucosal damage NF-κB p65