Tissue engineering strategy that combine biomaterials with living cells has shown special advantages in tissue regeneration and promoted the development of regenerative *** particular,the rising of 3D printing technol...
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Tissue engineering strategy that combine biomaterials with living cells has shown special advantages in tissue regeneration and promoted the development of regenerative *** particular,the rising of 3D printing technology further enriched the structural design and composition of tissue engineering scaffolds,which also provided convenience for cell loading and cell delivery of living *** this review,two types of cell-delivery scaffolds for tissue regeneration,including 3D printed scaffolds with subsequent cell-seeding and 3D cells bioprinted scaffolds,are mainly *** devote a major part to present and discuss the recent advances of two 3D printed cell-delivery scaffolds in regeneration of various tissues,involving bone,cartilage,skin tissues *** two types of 3D printed cell-delivery scaffolds have some shortcomings,they do have generally facilitated the exploration of tissue engineering scaffolds in multiple tissue *** is expected that 3D printed cell-delivery scaffolds will be further explored in function mechanism of seeding cells in vivo,precise mimicking of complex tissues and even organ reconstruction under the cooperation of multiple fields in future.
Objective:Estradiol (E2) plays an important role in the development of breast *** postmenopausal women,the estrogen can be synthesized via aromatase (CYP19) pathway and steroid-sulfatase (STS) pathway in periph...
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Objective:Estradiol (E2) plays an important role in the development of breast *** postmenopausal women,the estrogen can be synthesized via aromatase (CYP19) pathway and steroid-sulfatase (STS) pathway in peripheral tissues,when the production in ovary has *** objective of our study was to explore the effects of Shu-Gan-Liang-xue Decoction (SGLXD) on the expressions of CYP19 and STS in estrogen receptor positive breast cancer MCF-7 and T47D ***:The effects of SGLXD on the cell viability of MCF-7 and T47D were analyzed by MTT *** quantitative real-time RT-PCR and Western blot,we evaluated the mRNA and protein expressions of CYP19 and STS in MCF-7 and T47D cells after SGLXD ***:By MTT assay,the cell viability rates of MCF-7 and T47D were significantly inhibited by SGLXD in a dose-dependent manner,the IC50 values were 40.07 mg/ml for MCF-7 cells and 25.62 mg/ml for T47D cells,*** evidenced by real-time PCR and Western blot,the high concentrations of SGLXD significantly down-regulated the expressions of CYP19 and STS both in the transcript level and the protein ***:The results suggest that SGLXD is a potential dual aromatase-sulfatase inhibitor by simultaneously down-regulating the expressions of CYP19 and STS in MCF-7 and T47D cells.
Ban Xiao xue was announced as the China champion of the International Woolmark Prize (IWP) on July 12, Beijing, and will join the IWP global finalists in February 2013 during London Fashion Week.
Ban Xiao xue was announced as the China champion of the International Woolmark Prize (IWP) on July 12, Beijing, and will join the IWP global finalists in February 2013 during London Fashion Week.
Objective: To identify Kadsura interior(Dian Ji xue Teng, Schisandraceae) by using DNA ***: We analyzed five DNA barcodes(ITS, ITS2, psb A-trn H, mat K and rbc L) using DNA barcoding in terms of distance-based,tree-ba...
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Objective: To identify Kadsura interior(Dian Ji xue Teng, Schisandraceae) by using DNA ***: We analyzed five DNA barcodes(ITS, ITS2, psb A-trn H, mat K and rbc L) using DNA barcoding in terms of distance-based,tree-based and character-based identification to distinguish Kadsura interior and its ***: In distance-based and tree-based identification, K. interior could be distinguished easily from the species of Schisandra and K. *** character-based identification, there are two single nucleotide polymorphisms(SNPs) in ITS and one SNP in psb A-trn H which can be used to distinguish K. interior from K. heteroclita and K. ***: The results indicate that DNA barcoding can be used to identify K. interior. ITS and psb A-trnH sequence can be the most ideal DNA barcode for discriminating K. interior and its adulterants by the combination analysis of distance-based, tree-based and character-based identification(SNPs).
Prof. SHI xue-min is the first one who proposes the theory of quantitative needling manipulation. This theory requires corresponding quantitative marker of each point. In order to meet this quantitative requirement, h...
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Prof. SHI xue-min is the first one who proposes the theory of quantitative needling manipulation. This theory requires corresponding quantitative marker of each point. In order to meet this quantitative requirement, he set up strict and rigorous needling methods for each point. Part of his experience in this regard is introduced in this article.
Primary liver cancer is the most common malignant tumor of the ***,intervention,radiotherapy,and chemotherapy are the main treatment methods in the early stage,and the basic principles of post-treatment are palliative...
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Primary liver cancer is the most common malignant tumor of the ***,intervention,radiotherapy,and chemotherapy are the main treatment methods in the early stage,and the basic principles of post-treatment are palliative treatment and symptomatic *** Jing-Dong,a famous Chinese doctor,started from the etiology and pathogenesis of liver cancer,used the methods of promoting blood circulation to remove blood stasis,nourishing blood and replenishing liver,and applied Taohong Siwu Decoction based on long-term clinical experience to cure 1 case of primary liver cancer stage Ⅲa in two *** patient’s survival period can be prolonged and the quality of life can be *** author begins with the diagnosis of stage Ⅲa liver cancer,western medicine treatment methods,and previous treatment experience of traditional Chinese medicine,and briefly describes the diagnosis and treatment of this patient.
Objective: To investigate the neuroprotective effect of Bu-Shen-Huo-xue (BSHX) extract, a polyherbal formula, against High Glucose (HG)-induced neurotoxicity in PC12 cells. Methods: Cell viability assay, Lactate...
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Objective: To investigate the neuroprotective effect of Bu-Shen-Huo-xue (BSHX) extract, a polyherbal formula, against High Glucose (HG)-induced neurotoxicity in PC12 cells. Methods: Cell viability assay, Lactate Dehydrogenase (LDH) assay, Reactive Oxygen Species (ROS) detection, Hoechst 33258, Acridine Orange (AO)/Ethidium Bromide (EB) double stain and Mitochondrial Membrane Potential (MMP) assay were performed. In addition, Bax, Bcl-2, caspase-3, cleaved caspase-3, PARP, cleaved PARP, cytochrome c and Mitogen-Activated Protein Kinases (MAPKs) were detected by western blot. Results: BSHX extract increased cell viability and decreased LDH leakage in a concentration-dependent manner in HG-induced PC12 cells. Moreover, BSHX extract decreased the level of intracellular ROS, increased mitochondrial membrane potential, regulated the expressions of Bax and Bcl-2, and inhibited the release of cytochrome c from mitochondria. Furthermore, BSHX extract attenuated the activation of caspase-3 and PARP, and inhibited the phosphorylations of c-Jun N-terminal kinase (JNK) and p38 MAPKs. Conclusion: BSHX extract exhibited significant neuroprotective effect on HG-induced apoptosis in PC12 cells. This effect may be associated with the suppression of ROS generation as well as mitochondria-mediated caspase and JNK/p38 MAPK signaling pathways.
Axonal degeneration underlies many debilitating diseases including hereditary spastic paraplegia(HSP),a genetically and clinically diverse group of disorders characterized by spasticity and weakness of the lower *** i...
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Axonal degeneration underlies many debilitating diseases including hereditary spastic paraplegia(HSP),a genetically and clinically diverse group of disorders characterized by spasticity and weakness of the lower *** is one significant cause of chronic neurodisability due to the lack of effective treatments and a wide range of onset ages from early childhood to 70 years.
Turbulent agglomeration is viewed as a promising technology for enhancing fine particle removal *** better understand particle transport,agglomeration behaviors,and fluid-particle interactions,we numerically explored ...
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Turbulent agglomeration is viewed as a promising technology for enhancing fine particle removal *** better understand particle transport,agglomeration behaviors,and fluid-particle interactions,we numerically explored these phenomena under cylindrical vortex wake influence using a coupled large eddy simulation and discrete element method(LES-DEM) *** validity of the LES approach was verified by comparison with available direct numerical simulation(DNS) *** adopted the Johnson-Kendall-Roberts(JKR) contact model for particle-particle *** particle dispersion and agglomeration characteristics of particles with different diameters(dp=2-20 μm) in the laminar and transition of shear layer(TrSL) flow regimes were *** particles were concentrated at the vortex centers,while larger particles accumulated around the *** agglomeration efficiency exhibited an M-shaped profile spanwise(y-direction).With increasing Reynolds number,the agglomeration efficiency and turbulence intensity *** particle agglomeration efficiency peaks at a certain Reynolds ***,at higher Reynolds numbers,reducing the residence time of particles in the flow field decreases the agglomeration efficiency.
N6-methyladenosine(m^(6)A), the most prevalent and conserved RNA modification in eukaryotic cells, profoundly influences virtually all aspects of mRNA metabolism. mRNA plays crucial roles in neural stem cell genesis a...
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N6-methyladenosine(m^(6)A), the most prevalent and conserved RNA modification in eukaryotic cells, profoundly influences virtually all aspects of mRNA metabolism. mRNA plays crucial roles in neural stem cell genesis and neural regeneration, where it is highly concentrated and actively involved in these processes. Changes in m^(6)A modification levels and the expression levels of related enzymatic proteins can lead to neurological dysfunction and contribute to the development of neurological diseases. Furthermore, the proliferation and differentiation of neural stem cells, as well as nerve regeneration, are intimately linked to memory function and neurodegenerative diseases. This paper presents a comprehensive review of the roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, as well as its implications in memory and neurodegenerative diseases. m^(6)A has demonstrated divergent effects on the proliferation and differentiation of neural stem cells. These observed contradictions may arise from the time-specific nature of m^(6)A and its differential impact on neural stem cells across various stages of development. Similarly, the diverse effects of m^(6)A on distinct types of memory could be attributed to the involvement of specific brain regions in memory formation and recall. Inconsistencies in m^(6)A levels across different models of neurodegenerative disease, particularly Alzheimer's disease and Parkinson's disease, suggest that these disparities are linked to variations in the affected brain regions. Notably, the opposing changes in m^(6)A levels observed in Parkinson's disease models exposed to manganese compared to normal Parkinson's disease models further underscore the complexity of m^(6)A's role in neurodegenerative processes. The roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, and its implications in memory and neurodegenerative diseases, appear contradictory. These inconsistencies may be a
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