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Geometric and Electronic Behavior of C60 on PTCDA Hydrogen Bonded Network

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Chemical Research in Chinese Universities 2020年第1期36卷 81-85页

作者： LI Ling LI xuechao TANG Yanning xu zhichao ZHANG Haiming CHI Lifeng Institute of Functional Nano&Soft Materials(FUNSOM) Soochow UniversitySuzhou 215123P.R.China

Self-assembled supramolecular networks are promising spacer layer for electronic decoupling from the metal ***,the mechanism behind of how the intrinsic electronic structure of spacer layers affects the adsorbate is still *** a hydrogen bonded network composed of n-type semiconducting molecules 3,4,9,10-perylene-tetracarboxylic-dianhydride(PTCDA)is prepared under ultra-high vacuum to serve as a spacer layer for functional organics C60 on Au(111).The geometric and electronic information of C60 was investigated by scanning tunneling microscopy and scanning tunneling spectroscopy(STM/STS)at 5 *** decoupling from the metal surface yields an energy gap of 3.67 eV for C602nd,merely considering the HOMO-LUMO peak *** broadening of resonance peaks in STS measurements however indicates unneglected interlayer interactions in this hetero-organic ***,we scrutinize the nucleation sites of C60 on PTCDA layer and attribute this to the decreased diffusion capability on a less dense molecular arrangement possessing inhomogeneous spatial distribution of unoccupied molecular orbitals.

关键词： Electronic decoupling Scanning tunneling microscopy/spectroscopy C60,3,4,9,10-Perylene-tetracarbo-xylic-dianhydride(PTCDA)

Narrower atomic filter at 422.7 nm based on thermal Ca beam

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Chinese Science Bulletin 2014年第28期59卷 3543-3548页

作者： zhichao xu Xiaobo xue Duo Pan Xiaogang Zhang Wei Zhuang Jingbiao Chen State Key Laboratory of Advanced Optical Communication System and Network School of Electronics Engineering and Computer Science Peking University

So far, the only Ca magneto-optic atomic filters reported are based on a heated Ca cell, and the transmission spectrum obtained is double-peaked with 3 GHz effective passband limited by Doppler broadening. A magneto-optic atomic filter with single-peaked transmission spectrum of sub-Doppler bandwidth based on the thermal Ca atomic beam is firstly experimentally demonstrated. The fast Ca dipole transition(1S0–1P1) at 422.7 nm, which matches a strong solar Fraunhofer line, was utilized. A folded ninebeam traveling wave configuration is employed, and the maximum transmission efficiency is measured to be 7.3 %.A sub-Doppler transmission bandwidth of 590 MHz, much narrower than the 2.2 GHz Doppler width in the heated atomic cell, is obtained for the first time.

关键词：原子束过滤器纳米多普勒加宽钙传输效率原子滤波器多普勒宽度

Comparative Genome Analysis of Scutellaria baicalensis and Scutellaria barbata Reveals the Evolution of Active Flavonoid Biosynthesis

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Genomics, Proteomics & Bioinformatics 2020年第3期18卷 230-240页

作者： zhichao xu Ranran Gao Xiangdong Pu Rong xu Jiyong Wang Sihao Zheng Yan Zeng Jun Chen Chunnian He Jingyuan Song Key Lab of Chinese Medicine Resources Conservation State Administration of Traditional Chinese Medicine of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Engineering Research Center of Chinese Medicine Resource Ministry of EducationBeijing 100193China China National Traditional Chinese Medicine Co. LtdBeijing 102600China

Scutellaria baicalensis(***)and Scutellaria barbata(***)are common medicinal plants of the Lamiaceae *** produce specific flavonoid compounds,including baicalein,scutellarein,norwogonin,and wogonin,as well as their glycosides,which exhibit antioxidant and antitumor ***,we report chromosome-level genome assemblies of *** and *** with quantitative chromosomal variation(2 n=18 and 2 n=26,respectively).The divergence of *** and *** occurred far earlier than previously reported,and a whole-genome duplication(WGD)event was *** insertion of long terminal repeat elements after speciation might be responsible for the observed chromosomal expansion and *** genome analysis of the congeneric species revealed the species-specific evolution of chrysin and apigenin biosynthetic genes,such as the ***-specific tandem duplication of genes encoding phenylalanine ammonia lyase and chalcone synthase,and the ***-specific duplication of genes encoding 4-Co A *** addition,the paralogous duplication,colinearity,and expression diversity of CYP82 D subfamily members revealed the functional divergence of genes encoding flavone hydroxylase between *** and *** these Scutellaria genomes reveals the common and species-specific evolution of flavone biosynthetic ***,these findings would facilitate the development of molecular breeding and studies of biosynthesis and regulation of bioactive compounds.

关键词： Scutellaria Whole-genome duplication Flavonoid biosynthesis Tandem duplication Species-specific evolution

Effects of minocycline on learning and memory of mice following ischemic-hypoxic cerebral injuries

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Neural Regeneration Research 2012年第2期7卷 114-118页

作者： Hongling Fan Yuanyin Zheng Lijuan xu zhichao Zhong Shining Cai Shuling Zhang Quanzhong Chang Department of Physiology Zhuhai Campus of Zunyi Medical College Zhuhai 519041 Guangdong Province China

An ischemic-hypoxic animal model was established using right common carotid artery occlusions and inhalation of low concentrations of oxygen in mice. At 10 days after the ischemic-hypoxic injuries, saline-treated mice exhibited significantly prolonged escape latencies in water-maze tests and significantly shorter memory latencies and more mistakes in step-down tests. In contrast, mice treated with 5 mg/kg minocycline exhibited significant reversals of each of these effects compared with the saline-treated control mice. Moreover, we found that minocycline can relieve brain water content and morphological changes in mice following ischemic-hypoxic cerebral injuries. Accordingly, our findings indicate that minocycline provides some protections against the deleterious effects of these injuries in mice.

关键词： minocycline ischemic-hypoxic cerebral injury learning and memory cognition

A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets

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Virologica Sinica 2018年第2期33卷 131-141页

作者： zhichao xu Huiling Zhong Qingfeng Zhou Yunping Du Li Chen Yun Zhang Chunyi xue Yongchang Cao State Key Laboratory of Biocontrol School of Life ScienceSun Yat-sen University Guangdong Wen's Group Academy Guangdong Wen's Foodstuffs Group Co. Ltd School of Life Science Higher Education Mega Center Sun Yat-sen University

Porcine deltacoronavirus(PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and China's Mainland. Here we isolated a strain of PDCoV, named CHN-GD-2016,from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy. Genomic analysis showed that the PDCoV CHN-GD-2016 was closely related to other Chinese PDCoV strains, with the highest sequence similarity with the strain CHN/Tianjin/2016. Importantly, inoculation of newborn piglets with 1×10~5 TCID_(50) of CHN-GD-2016 by oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, and severe diarrhea in piglets. In addition, the virus RNA in rectal swabs from 1 to 7 days post inoculation was detected, macroscopic and microscopic lesions in small intestine were observed, and viral antigen was also detected in the small intestines with immunohistochemical staining. Collectively, the data show in this study confirms that PDCoV is present in Guangdong,China and is highly pathogenic in newborn piglets.

关键词： Porcine deltacoronavirus (PDCoV) Genomic analysis Pathogenicity Southern China

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春季冻融过程对河滨带湿地土壤酶活性的影响

生态环境学报 2024年第2期33卷 212-221页

作者：曹晓霭张睿温云浩王建徐智超田雅婷王立新刘华民内蒙古大学生态与环境学院内蒙古呼和浩特010021 草原生态安全省部共建协同创新中心内蒙古呼和浩特010021 蒙古高原生态学与资源利用教育部重点实验室内蒙古呼和浩特010021 内蒙古自治区生态环境督察技术支持中心内蒙古呼和浩特010011

土壤酶活性是土壤生态系统物质循环和能量流动的重要参与者,其活性大小对土壤养分和生化反应过程有明显指示作用。北方高纬度湿地经常受到冻融作用的影响,由于土壤温度的变化,土壤酶活性会发生变化。然而,春季冻融对土壤酶活性的影响机... 详细信息

土壤酶活性是土壤生态系统物质循环和能量流动的重要参与者,其活性大小对土壤养分和生化反应过程有明显指示作用。北方高纬度湿地经常受到冻融作用的影响,由于土壤温度的变化,土壤酶活性会发生变化。然而,春季冻融对土壤酶活性的影响机制仍不清楚。为探讨春季冻融过程对河滨带湿地土壤理化性质和土壤酶活性的影响,以及土壤理化性质与土壤酶活性间的关系,选取锡林河河滨带分布最广泛的芦苇(Phragmites australis)和灰脉苔草(Carex appendiculata)这2种典型湿地植物群落为研究对象,分析春季冻融期间土壤脲酶、蔗糖酶、过氧化氢酶活性的变化及其影响因素。在冻结期,河滨带湿地土壤酶活性均保持较高活性,土壤脲酶和蔗糖酶活性在冻融期均出现迅速增高而后降低的趋势,并在土壤融化期又升高,然而土壤过氧化氢酶对冻融作用的响应较小。在冻融过程中,芦苇覆盖下土壤蔗糖酶和过氧化氢酶活性显著高于灰脉苔草群落且表现出明显的空间异质性。通径分析结果表明,春季冻融期河滨带湿地土壤脲酶活性与土壤温度、总碳、总氮显著正相关,土壤蔗糖酶与总碳、总氮呈显著正相关;过氧化氢酶活性除与土壤温度没有显著相关性,与其他理化因子均有显著相关性。冻融期不同植物群落下土壤酶活性与河滨带湿地碳、氮含量密切相关,频繁的冻融对土壤酶活性以及河滨带湿地土壤物质循环产生重要影响。该研究探讨了3种土壤酶活性对春季冻融过程的响应及在植物群落间的差异,为河滨带湿地生态系统碳、氮等营养物质的循环提供理论依据。

关键词：河滨带湿地冻融交替土壤酶活性植物群落锡林河通径分析

Characteristic stress variation and microcrack evolution of granite subjected to uniaxial compression using acoustic emission methods

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第9期16卷 3511-3523页

作者： Yunge Zhao Xibing Li Linqi Huang zhichao Zhang Zihou xu School of Resources and Safety Engineering Central South UniversityChangsha410083China

The size of mineral grain has a significant impact on the initiation and propagation of microcracks within *** this study,fine-,medium-,and coarse-grained granites were used to investigate microcrack evolution and characteristic stress under uniaxial compression using the acoustic emission(AE),digital image correlation(DIC),and nuclear magnetic resonance(NMR)*** experimental results show that the characteristic stress of each granite decreased considerably with increasing grain *** inflection points of the b-value occurred earlier with an increase in grain sizes,indicating that the larger grains promote the generation and propagation of *** distribution characteristics of the average frequency(AF)and the ratio of rise time to amplitude(RA)indicate that the proportion of shear microcracks increases with increasing grain *** NMR results indicate that the porosity and the proportion of large pores increased with increasing grain size,which may intensify the microcrack ***,analysis of the DIC and AE event rates suggests that the high-displacement regions could serve as a criterion for the degree of microcrack *** study found that granites with larger grains had a higher proportion of high-displacement regions,which can lead to larger-scale cracking or even *** findings are not only beneficial to understand the pattern of microcrack evolution with different grain sizes,but also provide guidance for rock monitoring and instability assessment.

关键词： Grain size Acoustic emission(AE) Characteristic stress Microcrack evolution Fracture mode

The Wolfiporia cocos Genome and Transcriptome Shed Light on the Formation of Its Edible and Medicinal Sclerotium

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Genomics, Proteomics & Bioinformatics 2020年第4期18卷 455-467页

作者： Hongmei Luo Jun Qian zhichao xu Wanjing Liu Lei xu Ying Li Jiang xu Jianhong Zhang Xiaolan xu Chang Liu Liu He Jianqin Li Chao Sun Francis Martin Jingyuan Song Shilin Chen Engineering Research Center of Chinese Medicine Resource Ministry of EducationInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China College of Pharmacy Hubei University of Chinese MedicineWuhan 430065China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China INRA Universitéde LorraineUMR 1136 Interactions Arbres/Microorganismes54280 ChampenouxFrance Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Institute of MicrobiologyBeijing Forestry UniversityBeijing 100083China

Wolfiporia cocos(F. A. Wolf) has been praised as a food delicacy and medicine for centuries in China. Here, we present the genome and transcriptome of the Chinese strain CGMCC5.78 of W. cocos. High-confidence functional prediction was made for 9277 genes among the 10,908 total predicted gene models in the W. cocos genome. Up to 2838 differentially expressed genes(DEGs)were identified to be related to sclerotial development by comparing the transcriptomes of mycelial and sclerotial tissues. These DEGs are involved in mating processes, differentiation of fruiting body tissues, and metabolic pathways. A number of genes encoding enzymes and regulatory factors related to polysaccharide and triterpenoid production were strikingly regulated. A potential triterpenoid gene cluster including the signature lanosterol synthase(LSS) gene and its modified components were annotated. In addition, five nonribosomal peptide synthase(NRPS)-like gene clusters,eight polyketide synthase(PKS) gene clusters, and 15 terpene gene clusters were discovered in the genome. The differential expression of the velevt family proteins, transcription factors,carbohydrate-active enzymes, and signaling components indicated their essential roles in the regulation of fungal development and secondary metabolism in W. cocos. These genomic and transcriptomic resources will be valuable for further investigations of the molecular mechanisms controlling sclerotial formation and for its improved medicinal applications.

关键词： Wolfiporia cocos Genome sequencing Sclerotial formation Fungal development Polysaccharide and triter-penoid biosynthesis

Cannabis sativa: origin and history, glandular trichome development, and cannabinoid biosynthesis

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Horticulture Research 2023年第9期10卷 306-320页

作者： Ziyan Xie Yaolei Mi Lingzhe Kong Maolun Gao Shanshan Chen Weiqiang Chen Xiangxiao Meng Wei Sun Shilin Chen zhichao xu Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040China College of Life Science Northeast Forestry UniversityHarbin 150040China Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Institute of Herbgenomics Chengdu University of Traditional Chinese MedicineChengdu 611137China

Is Cannabis a boon or bane?Cannabis sativa has long been a versatile crop for fiber extraction(industrial hemp),traditional Chinese medicine(hemp seeds),and recreational drugs(marijuana).Cannabis faced global prohibition in the twentieth century because of the psychoactive properties of △^(9)-tetrahydrocannabinol;however,recently,the perspective has changed with the recognition of additional therapeutic values,particularly the pharmacological potential of cannabidiol.A comprehensive understanding of the underlying mechanism of cannabinoid biosynthesis is necessary to cultivate and promote globally the medicinal application of Cannabis ***,we comprehensively review the historical usage of Cannabis,biosynthesis of trichome-specific cannabinoids,regulatory network of trichome development,and synthetic biology of *** review provides valuable insights into the efficient biosynthesis and green production of cannabinoids,and the development and utilization of novel Cannabis varieties.

关键词： comprehensive versatile globally