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秋水仙碱对胶质瘤细胞系化疗敏感性的影响

中国临床神经外科杂志 2024年第2期29卷 97-104页

作者：吴宇朱馨艺江洪祥陈谦学武汉大学人民医院神经外科武汉430060

目的探讨秋水仙碱对胶质瘤细胞系化疗敏感性的影响及其机制。方法采用不同浓度的替莫唑胺(TMZ)处理人脑胶质瘤细胞系U87和A172细胞,构建TMZ耐药细胞系U87/TR和A172/TR,再使用秋水仙碱(10、30 ng/ml)干预1、2、3 d,采用细胞计数法、BrdU... 详细信息

目的探讨秋水仙碱对胶质瘤细胞系化疗敏感性的影响及其机制。方法采用不同浓度的替莫唑胺(TMZ)处理人脑胶质瘤细胞系U87和A172细胞,构建TMZ耐药细胞系U87/TR和A172/TR,再使用秋水仙碱(10、30 ng/ml)干预1、2、3 d,采用细胞计数法、BrdU法和流式细胞术检测细胞活力、细胞增殖和细胞周期。生物信息学方法分析胶质瘤组织差异表达的微小RNA(miRNAs),并预测其靶基因;然后,采用qRT-PCR检测U-87/TR和A172/TR细胞中这些miRNAs的表达情况,调控miRNAs的表达以观察秋水仙碱效应的变化。结果秋水仙碱显著抑制U87/TR和A172/TR细胞的增殖活力(P<0.05),呈时间依赖性和剂量依赖性(P<0.05),而且显著阻滞U87/TR和A172/TR的有丝分裂,使细胞阻滞在G0/G1期。30ng/ml秋水仙碱显著增加TMZ对U87/TR和A172/TR细胞增殖的抑制效果(P<0.05),明显降低TMZ的IC50值(P<0.05)。生信分析显示,胶质瘤组织miR-330-3p、miR-491-5p、miR-6782-5p、miR-31-5p、miR-330-5p、miR-137和miR-433-3p呈差异表达,秋水仙碱明显上调U87/TR和A172/TR细胞miR-330-3p的表达(P<0.05)、明显抑制miR-330-3p靶基因ErbB的表达(P<0.05)。抑制miR-330-3p表达明显逆转秋水仙碱的生物学效应(P<0.05)。结论秋水仙碱可以抑制TMZ耐药的胶质瘤细胞的增殖,其机制可能涉及到miR-330-3p/ErbB信号通路的调节。

关键词：胶质瘤 U87细胞 A172细胞替莫唑胺秋水仙碱化疗敏感性

Antigen-presenting effects of effector memory Vγ9Vδ2 T cells in rheumatoid arthritis

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Cellular & Molecular Immunology 2012年第3期9卷 245-254页

作者： Chaoying Hu Liu qian yi Miao Qiuyu Huang Ping Miao Ping Wang Qiwen Yu Hong Nie Jiying Zhang Dongyi He Rong xu xuehua Chen Bingya Liu Dongqing Zhang Shanghai Institute of Immunology Shanghai Jiao Tong University School of MedicineShanghaiChina Central laboratory Shanghai Xuhui Central HospitalShanghaiChina Guanghua Rheumatoid Arthritis Specialized Hospital ShanghaiChina Shanghai Jiao Tong University School of Medicine Ruijin HospitalShanghaiChina

Rheumatoid arthritis is an autoimmune disease that primarily affects the limbs, but the pathogenic mechanism remains unclear. 78 T cells, a T-cell subpopulation, are characterized by multiple biological functions and associated with a variety of diseases. This study investigated the antigen-presenting effects of γδ2 cells and their relationship with rheumatoid arthritis development. We found that Vγ9Vδ2 T cells （the predominant subtype of γδ T cells in peripheral blood） were activated by isopentenyl pyrophosphate to continuously proliferate and differentiate into effector memory cells. The effector memory Vγ9Vδ2 T cells exhibited phenotypic characteristics of specific antigen-presenting cells, including high HLA-DR and CD80/86 expression. These Vγ9Vδ2 T cells could present soluble antigens and synthetic peptides to CD4＋ T cells. Vγ9Vδ2 T cells with different phenotypes showed different cytokine secretion patterns. Effector memoryVγ9Vδ2 T cells simultaneously secreted not only interferon （IFN）-γbut also IL-17. The peripheral blood and joint synovial fluid from RA patients contained numerous heterogeneous γδ T cells that were predominantly effector memory Vγ9Vδ2 T cells with the ability to secrete inflammatory factors. We also found that γδ T cells had a similar antigen-presenting capability to B cells. These results suggest that during the development of rheumatoid arthritis, 78 T cells can aggravate immune dysfunction and produce abnormal immune damage by secreting cytokines and inducing inflammatory cells to participate in synergistic inflammatory responses. Furthermore, γδ T cells can behave similarly to B cells to present viral peptides and autoantigen peptides to CD4＋ T cells, thus sustaining CD4＋ T-cell activation.

关键词： antigen-presenting function rheumatoid arthritis γδT cell TEM Vγ9Vδ2 T cell

双β-二酮钛系烯烃聚合催化剂的合成、表征及其催化乙烯聚合

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当代化工 2024年第1期53卷 137-140页

作者：高美怡张丹李君华钱建华辽宁石油化工大学辽宁抚顺113001

合成了一种新型β-二酮钛配合物[(acac)_(2)Ti(C_(6)H_(6))_(2)(3a)、(acac)_(2)Ti(C_(6)H_(5)OCH_(3))_(2)(3b)、(acac)_(2)Ti(C_(6)H_(5)CF_(3))_(2)(3c)],采用^(1)H NMR、^(13)C NMR、元素分析等方法进行表征。用配合物作催化剂,以甲基铝氧烷(MAO)为助催化剂催化乙烯聚合,研究了聚合条件(如Al/Ti摩尔比、聚合温度和催化剂结构)对催化活性的影响。其中3a对乙烯聚合活性达6.26×10^(5)g·mol^(-1)·h^(-1),聚合物最大的重均分子量M_(w)为13.47 kg·mol^(-1),分子量分布PDI为2.0。

关键词： β-二酮钛系乙烯聚合 [O,O]双齿配体

Carbazole-Based Donor-Acceptor Conjugated Microporous Polymers for Efficient Visible-Light-Driven Photocatalytic H_(2) Evolution

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Chinese Journal of Chemistry 2023年第19期41卷 2518-2524页

作者： Linchu xu Chen Yang Zhonghua Cheng Qihang Huang Shizhe Zhang Cheng qian Yaozu Liao State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai201620 China

Conjugated microporous polymers (CMPs) featuring extended π-structures, large specific surface area and tailor-made functionalities are a class of promising organic photocatalysts for hydrogen evolution reaction (HER) from water. However, the photocatalytic activities of most CMPs are severely hindered by slow charge transfer rate and fast charge recombination process. Herein, we develop a strategy for the synthesis of donor-acceptor CMPs through nickel(0)-catalyzed Yamamoto cross-coupling of 3,6-dibromo-9-(4-bromophenyl)carbazole (CZ) with 5,5'-dibromo-2,2'-bipyridine (DBPy) for efficient HER from water. The PCZN-4 prepared with a 2 : 3 stoichiometric ratio of CZ to DBPy exhibited the highest photocatalytic hydrogen evolution rate of 7160 μmol·g^(–1)·h^(–1), which was nearly equal to 179 times and 143 times that of PCZN-1 (40 μmol·g^(–1)·h^(–1)) and PCZN-6 (50 μmol·g^(–1)·h^(–1)) obtained by Yamamoto homocoupling of CZ and DBPy, respectively. Compared to the homocoupling counterparts, the enhanced photocatalytic activity of PCZN-4 results from improved separation efficiency of charge carriers. Interestingly, the photocatalytic H2 evolution performance of PCZN-4 could be further improved up to 17080 μmol·g^(–1)·h^(–1) by adjusting pH of the aqueous solution. This work offers a novel approach for improving photocatalytic efficiency by tuning the chemical structures and surrounding microenvironment of the polymer backbone.

关键词： Carbazole Bipyridine Donor-acceptor Microenvironment Conjugated polymers Photocatalytic hydrogen evolution

Enhanced electrode kinetics and properties via anionic regulation in polyanionic Na_(3+x)V_(2)(PO_(4))_(3-x)(P_(2)O_(7))_(x) cathode material

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Green Energy & Environment 2022年第4期7卷 763-771页

作者： Mei-yi Wang Xin-Xin Zhao Jin-Zhi Guo xue-Jiao Nie Zhen-yi Gu xu Yang Xing-Long Wu National&Local United Engineering Laboratory for Power Batteries Faculty of ChemistryNortheast Normal UniversityChangchunJilin130024China Key Laboratory for UV Light-Emitting Materials and Technology of Ministry of Education Northeast Normal UniversityChangchunJilin130024China

Mixing polyanion cathode materials are promising candidates for the development of next-generation batteries, owing to their structural robustness and low-volume changes, yet low conductivity of polyanion hinders their practical capacity. Herein, the anion-site regulation is proposed to elevate the electrode kinetics and properties of polyanionic cathode. Multivalent anion P_(2)O_(7)^(4-) is selected to substitute the PO_(4)^(3-) in Na_(3)V_(2)(PO_(4))_(3) (NVP) lattice and regulate the ratio of polyanion groups to prepare Na_(3+x)V_(2)(PO_(4))_(3-x)(P_(2)O_(7))_(x)(NVPP_(x), 0 ≤ x ≤ 0.15) *** optimal Na_(3.1)V_(2)(PO_(4))_(2.9)(P_(2)O_(7))_(0.1) (NVPP_(0.1)) material can deliver remarkably elevated specific capacity(104 mAh g^(-1) at 0.1 C, 60 mAh g^(-1) at 20 C, respectively), which is higher than those of NVP. Moreover, NVPP_(0.1) exhibits outstanding cyclic stability(91% capacity retention after 300 cycles at 1 C). Experimental analyses reveal that the regulation of anions improves the structure stability, increases the active Na occupancy in the lattice and accelerates the Na+migration kinetics. The strategy of anion-site regulation provides the researchers a reference for the design of new high-performance polyanionic materials.

关键词： Sodium-ion batteries Cathode Polyanion materials Anionic substitution Electrode kinetics

重庆涪陵页岩气开采区麻溪河流域水和沉积物多环芳烃分布、来源及风险评价

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环境科学学报 2024年第4期44卷 133-144页

作者：张超秦超杰范希周俊鹏钱喆杨秀雯易佳佩赵亚辉刘伟祁士华陈伟中国地质大学(武汉)环境学院武汉430078 中国地质大学(武汉)地质调查研究院武汉430074 中国地质大学(武汉)生物地质与环境地质国家重点实验室武汉430078 中国地质大学(武汉)长江水科学湖北省重点实验室武汉430078

页岩气开采过程中产生的多环芳烃(PAHs)可能通过多种途径对开采区流域水环境产生威胁.为探明页岩气开采活动对周边水环境中PAHs的影响,本研究选取重庆涪陵麻溪河流域为研究对象,分析了区域内地表水及沉积物中PAHs的含量、组成特征和空... 详细信息

页岩气开采过程中产生的多环芳烃(PAHs)可能通过多种途径对开采区流域水环境产生威胁.为探明页岩气开采活动对周边水环境中PAHs的影响,本研究选取重庆涪陵麻溪河流域为研究对象,分析了区域内地表水及沉积物中PAHs的含量、组成特征和空间分布,对其污染来源进行了解析及对其生态风险进行了评估.结果表明,地表水及沉积物中PAHs的浓度范围分别为0.19~119 ng·L^(-1)、12.1~418 ng·g^(-1),处于低至中等污染水平.全流域地表水中PAHs呈麻溪河上游>枧溪河>麻溪河下游>麻溪河中游的空间分布特点;地表沉积物主要受到页岩气开采的影响,PAHs空间分布主要呈现麻溪河中游>麻溪河上游>枧溪河>麻溪河下游的特点.地表水及沉积物中PAHs呈现以低分子量PAHs为主的组成特征.特征比值和主成分分析的结果表明,PAHs主要来自石油源和石油类产品燃烧源.生态风险评估表明流域内部分PAH单体处于中风险水平,高分子量PAHs对水体生态风险贡献较大,低分子量PAHs对沉积物生态风险贡献较大.总体上,麻溪河水体中总PAHs呈中等生态风险等级;地表沉积物中PAHs处于生态环境低风险毒性,对生物造成的有害影响不明显.本研究结果为涪陵页岩气开产区PAHs的污染控制和风险评价提供了理论指导和技术支撑.

关键词：多环芳烃页岩气开采区地表水环境污染特征来源解析生态风险

Prolonged stay of spontaneous intracranial hemorrhage patients in the emergency department is correlated with worse outcomes

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World Journal of Emergency Medicine 2022年第3期13卷 222-225页

作者： qian He Rui Guo Ke Zhang Chao You yi Liu Zhi Zeng Department of Emergency West China HospitalSichuan UniversityChengdu 610041China Department of Neurosurgery West China HospitalSichuan UniversityChengdu 610041China Department of Neurosurgery the First People's Hospital in Shuangliu Distract/West China Airport HospitalSichuan UniversityChengdu 610041China

Spontaneous intracerebral hemorrhage(ICH)represents the second most common type of stroke,with high mortality and disability rates.^([1,2])In 2010,there were approximately 5.3 million ICH cases,with 3 million deaths worldwide.^([1])However,there is still no validated medical treatment for ICH,with the role of surgery remaining controversial.^([2,3])Cusack et al^([4])demonstrated that lowering blood pressure rapidly in hypertensive ICH patients may be safe and at least partially effective in inhibiting hematoma expansion.

关键词： patients hypertensive mortality

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几种杀菌剂对元胡霜霉病田间防治效果

农药 2024年第4期63卷 293-295,299页

作者：柳采秀王松琳金怡宗侃侃徐笔奇苍涛浙江省农业科学院农产品质量安全与营养研究所杭州310021 浙江省农业技术推广中心杭州311200 东阳市良种推广中心浙江东阳322100 磐安县中药产业发展促进中心浙江磐安322300

[目的]为了筛选出对元胡霜霉病安全高效的杀菌剂。[方法]采用田间小区试验方法测定了8种杀菌剂在试验设计剂量下对元胡霜霉病的田间防治效果以及对元胡的安全性。[结果]各试验小区的元胡生长正常,无明显影响;70%氟吡菌胺·霜脲氰水分散... 详细信息

[目的]为了筛选出对元胡霜霉病安全高效的杀菌剂。[方法]采用田间小区试验方法测定了8种杀菌剂在试验设计剂量下对元胡霜霉病的田间防治效果以及对元胡的安全性。[结果]各试验小区的元胡生长正常,无明显影响;70%氟吡菌胺·霜脲氰水分散粒剂、23.4%双炔酰菌胺悬浮剂、722 g/L霜霉威盐酸盐水剂3种药剂对元胡霜霉病表现出较好防效,防治效果分别为83.3%、78.5%和73.6%;100 g/L氰霜唑悬浮剂、80%三乙膦酸铝水分散粒剂和80%烯酰吗啉可湿性粉剂3种生产中常用药剂相对表现不佳,防效分别为43.6%、50.9%和51.3%。[结论]70%氟吡菌胺·霜脲氰水分散粒剂和23.4%双炔酰菌胺悬浮剂对元胡霜霉病的防治效果优于722 g/L霜霉威盐酸盐水剂,在防治元胡霜霉病的过程中可推荐使用。

关键词：元胡霜霉病防治效果病叶率

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基于冲蚀-空蚀分析的切断阀流道结构研究

阀门 2024年第9期 1114-1119页

作者：汪栩涵刘闯金志江钱锦远浙江大学化工机械研究所浙江杭州310027

在正常使用过程中,切断阀内部通常存在多相流,造成冲蚀和空蚀,使切断阀受到损伤,导致调节能力下降、使用寿命降低。针对这一现状,本文通过建立流动分析模型、空蚀模型和冲蚀模型,以数值仿真的手段对某一切断阀在不同开度下内部流场、空... 详细信息

在正常使用过程中,切断阀内部通常存在多相流,造成冲蚀和空蚀,使切断阀受到损伤,导致调节能力下降、使用寿命降低。针对这一现状,本文通过建立流动分析模型、空蚀模型和冲蚀模型,以数值仿真的手段对某一切断阀在不同开度下内部流场、空化情况和冲蚀磨损分布开展研究,得到了切断阀的流场分布和流量特性曲线,发现空蚀主要位于切断阀出口壁面,而冲蚀在入口和出口均有分布。这对后续阀门结构和工况参数的改进具有一定指导意义。

关键词：切断阀空蚀冲蚀多相流数值仿真