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乳酸诱导PLEKHA4的上调参与胶质瘤细胞增殖和凋亡的生物学进程

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南方医科大学学报 2023年第7期43卷 1071-1080页

作者：叶静静徐文琴奚邦生王能乾陈天兵皖南医学院重大疾病非编码RNA转化研究安徽普通高校重点实验室安徽芜湖241001 皖南医学院弋矶山医院中心实验室安徽芜湖241001 皖南医学院弋矶山医院检验科安徽芜湖241001 皖南医学院弋矶山医院儿科安徽芜湖241001

目的探究乳酸诱导的PLEKHA4基因表达对胶质瘤细胞的生物学功能的影响以及潜在分子机制。方法通过GEO数据库以及GEPIA2在线网站分析PLEKHA4表达水平与胶质瘤病理分级的关系,通过RNA小干扰技术设计PLEKHA4 siRNA后分别转染胶质瘤U251和T98... 详细信息

目的探究乳酸诱导的PLEKHA4基因表达对胶质瘤细胞的生物学功能的影响以及潜在分子机制。方法通过GEO数据库以及GEPIA2在线网站分析PLEKHA4表达水平与胶质瘤病理分级的关系,通过RNA小干扰技术设计PLEKHA4 siRNA后分别转染胶质瘤U251和T98G细胞处理1 d,通过实时细胞分析技术以及Edu实验检测胶质瘤细胞的增殖能力,平板克隆实验检测细胞的克隆形成能力,流式分析技术检测胶质瘤细胞的周期和凋亡;PCR检测临床收集的胶质瘤样本和对照以及乳酸和葡萄糖治疗下胶质瘤细胞中PLEKHA4的mRNA表达量的变化;体内异种移植小鼠实验检测裸鼠成瘤能力;Westernblot检测cyclinD1、CDK2、Bcl2、β-catenin表达和MAPK信号通路关键蛋白的磷酸化表达水平。结果GEO数据库和在线网站分析结果显示PLEKHA4在胶质瘤组织中高表达且与不良预后相关;RTCA以及Edu实验显示,敲降PLEKHA4抑制胶质瘤细胞的增殖(P<0.05);平板克隆实验显示,敲降组的细胞克隆数目明显低于对照组(P<0.01);流式细胞术分析结果显示敲低PLEKHA4后细胞周期受到阻滞G1期细胞增加,S期细胞减少,凋亡细胞增加(P<0.01);胶质瘤样本和乳酸和葡萄糖治疗后胶质瘤细胞的PLEKHA4基因的mRNA表达升高(P<0.01);敲降PLEKHA4基因后,裸鼠成瘤能力降低;Westernblot结果显示敲减PLEKHA4后能够抑制cyclinD1、CDK2、Bcl2等功能蛋白的表达,且显著抑制ERK、p38的磷酸化和β-catenin蛋白表达(P<0.01)。结论敲减PLEKHA4基因通过抑制MAPK信号通路的活化和β-Catenin的表达,抑制胶质瘤细胞的增殖促进凋亡,同时糖酵解产生的乳酸诱导PLEKHA4表达上调。

关键词：胶质瘤乳酸 PLEKHA4 增殖凋亡生物学功能

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等离子体表面改性对PTFE织物自润滑衬垫综合性能的影响

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轴承 2022年第4期 59-62,82页

作者：何俊惠庄彩虹徐文钦陈志雄福建龙溪轴承(集团)股份有限公司福建漳州363000 福建省关节轴承企业重点实验室福建漳州363000

采用等离子体对PTFE织物进行表面改性,通过接触角测试、顶破强力、剥离强度和摩擦磨损寿命试验研究等离子体表面改性对PTFE织物亲水性、PTFE织物自润滑衬垫整体强度、剥离强度和摩擦磨损性能的影响。试验结果表明:等离子体表面改性后PTF... 详细信息

采用等离子体对PTFE织物进行表面改性,通过接触角测试、顶破强力、剥离强度和摩擦磨损寿命试验研究等离子体表面改性对PTFE织物亲水性、PTFE织物自润滑衬垫整体强度、剥离强度和摩擦磨损性能的影响。试验结果表明:等离子体表面改性后PTFE织物与水的接触角由135.9°降至91.8°,树脂对织物的浸润效果得到了改善;等离子体表面改性后PTFE织物衬垫的平均顶破强力为未处理衬垫的1.59倍;平均剥离强度为未处理衬垫的2.07倍;平均摩擦磨损寿命为未处理衬垫的1.66倍,衬垫的整体强度和耐磨性均得到了提高。

关键词：滑动轴承关节轴承等离子体表面改性衬垫接触角剥离强度耐磨性

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Mitophagy in acute central nervous system injuries:regulatory mechanisms and therapeutic potentials

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Neural Regeneration Research 2025年第9期20卷 2437-2453页

作者： Siyi xu Junqiu Jia Rui Mao Xiang Cao Yun xu Department of Neurology Nanjing Drum Tower HospitalClinical College of Jiangsu UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalChinese Academy of Medical Science&Peking Union Medical CollegeNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina State Key Laboratory of Pharmaceutical Biotechnology and Institute of Translational Medicine for Brain Critical Diseases Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina Nanjing Neurology Medical Center NanjingJiangsu ProvinceChina

Acute central nervous system injuries,including ischemic stro ke,intracerebral hemorrhage,subarachnoid hemorrhage,traumatic brain injury,and spinal co rd injury,are a major global health *** optimal therapies and improving the long-term neurological functions of patients with acute central nervous system injuries are urgent *** are susceptible to damage after acute central nervous system injury,and this leads to the release of toxic levels of reactive oxygen species,which induce cell ***,a selective form of autophagy,is crucial in eliminating redundant or damaged mitochondria during these *** evidence has highlighted the significant role of mitophagy in acute central nervous system *** this review,we provide a comprehensive overview of the process,classification,and related mechanisms of *** also highlight the recent developments in research into the role of mitophagy in various acute central nervous system injuries and drug therapies that regulate *** the final section of this review,we emphasize the potential for treating these disorders by focusing on mitophagy and suggest future research paths in this area.

关键词： autophagy intracerebral hemorrhage ischemic stroke mitochondria mitochondrial biogenesis mitochondrial quality control mitophagy spinal cord injury subarachnoid hemorrhage traumatic brain injury

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4,4'-二硝基二苯基脲的合成研究进展

化工生产与技术 2022年第4期28卷 26-29,I0004页

作者：徐文琴苏为科浙江工业大学药学院浙江310014

介绍了尼卡巴嗪中间体4,4'-二硝基二苯基脲的4种合成工艺路线,根据原料来源不同可分为光气法、异氰酸酯法、尿素法和三光气法。主要利用对硝基苯胺和光气、对硝基苯异氰酸酯、尿素以及三光气为原料合成4,4'-二硝基二苯基脲,分析了这些... 详细信息

介绍了尼卡巴嗪中间体4,4'-二硝基二苯基脲的4种合成工艺路线,根据原料来源不同可分为光气法、异氰酸酯法、尿素法和三光气法。主要利用对硝基苯胺和光气、对硝基苯异氰酸酯、尿素以及三光气为原料合成4,4'-二硝基二苯基脲,分析了这些合成路线的优缺点,分析表明,以对硝基苯胺和三光气为原料的合成工艺路线所需反应时间短,收率和纯度较高,适用于工业化生产,但该工艺需使用有机碱为缚酸剂且后处理较复杂,因此亟待提出一种消除或减少缚酸剂的绿色环保合成工艺路线。

关键词： 4,4'-二硝基二苯基脲合成工艺有机中间体

Applications of in-situ solid-state nuclear magnetic resonance in methanol to olefins reaction

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Science China Chemistry 2025年第1期68卷 134-151页

作者： Jing Niu Shutao xu Yingxu Wei Zhongmin Liu National Engineering Research Center of Lower-Carbon Catalysis Technology Dalian Institute of Chemical PhysicsChinese Academy of Sciences State Key Laboratory of Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences

The methanol-to-olefins（MTO） reaction offers an alternative pathway for the production of low-carbon olefins from non-oil feedstocks. Fundamental research has been impeded by a lack of comprehensive understanding of its underlying mechanism,despite the significant progress made in industry. In-situ solid-state nuclear magnetic resonance（ss NMR） spectroscopy has emerged as a pivotal tool, offering crucial insights into key species under real-time operando conditions. Furthermore, the host–guest interaction between zeolites or surface species residing on zeolites and the reactant/active intermediates is revealed by the combination of in-situ13C MAS NMR and 2D correlation spectroscopy. Moreover, recent technological advancements in hyperpolarization（HP） methods, including HP129Xe NMR and dynamic nuclear polarization（DNP）, have significantly improved the sensitivity of ss NMR, enabling detailed structural and kinetic analysis as well as the detection of trace species. In this feature article, we summarized recent advancements in（in-situ） ss NMR spectroscopy applied to MTO reaction processes,encompassing mechanistic investigations at various stages and the intricate host–guest interactions. These theoretical insights into the dynamic evolution of MTO reactions lay a solid foundation for the optimization of catalytic processes and the development of efficient catalysts, thereby advancing the techniques towards more sustainable and economical production route for olefins.

关键词： MTO zeolites in-situ ssNMR reactive intermediates reaction mechanism

闸控河道水污染物通量在线监测系统建设与应用

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环境监测管理与技术 2024年第2期36卷 56-59页

作者：郭有顺许文卿张鹏杨佘维姚晨辉广东省环境科学研究院广东广州510045 广东环科院环境科技有限公司广东广州510045

以练江流域(普宁段)为例,分析了水污染物通量在线监测系统的需求与建设目标,介绍了系统总体架构、数据库、系统功能、监测布点和感知设备配置等设计要点,确定了系统中水污染物通量的计算方法。通过水污染物通量的在线监测与智能分析,实... 详细信息

以练江流域(普宁段)为例,分析了水污染物通量在线监测系统的需求与建设目标,介绍了系统总体架构、数据库、系统功能、监测布点和感知设备配置等设计要点,确定了系统中水污染物通量的计算方法。通过水污染物通量的在线监测与智能分析,实现对河流水质的自动研判和预警,使系统在流域综合治理工作中发挥作用。

关键词：污染物通量在线监测流域治理闸控河道

Approach from the laboratory to the field: New strategies in the control of mosquito-borne infectious diseases

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Science China Life Sciences 2025年第1期68卷 303-305页

作者： Ziqian xu Lianpan Dai Jun Liu National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases (NITFID) National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences

Vector-borne viruses, transmitted among humans and animal hosts through bites of vectors such as mosquitoes and ticks,pose a great and ongoing threat to public health and biosafety(Yang et al., 2022).Among them, dengue virus-induced epidemics, have dramatically increased worldwide in recent decades. The report released by the World Health Organization(WHO) at the end of 2023 showed that dengue epidemic globally surged in2023, with the number of reported cases nearing a historical peak.

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Enhancing insights into Global Cancer Statistics 2022: implications for cancer control

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Science China Life Sciences 2025年第1期68卷 294-296页

作者： Jingtong xu Runtian Wang Xiaoxiang Guan Department of Oncology the First Affiliated Hospital of Nanjing Medical University Jiangsu Key Lab of Cancer Biomarkers Prevention and TreatmentCollaborative Innovation Center for Personalized Cancer MedicineNanjing Medical University

Globally, there has been a marked escalation in tumor morbidity and mortality,cementing tumors as the predominant cause of death in numerous *** the current tumor burden across various regions is imperative to discern potential risk factors and formulate efficacious strategies for tumor ***, the International Agency for Research on Cancer(IARC) provided a status report on the global tumor burden,estimating the incidence and mortality of 36 cancers in 185 countries(Bray et al.,2024).

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Copper-catalyzed C–H bond functionalization enabled by programmed alternating current

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Science China Chemistry 2025年第1期68卷 10-11页

作者： Zhoumei Tan Kun xu Chengchu Zeng Beijing Key Laboratory of Environmental and Viral Oncology College of Chemistry and Life Science Beijing University of Technology

Synthetic electrochemistry has been selected as one of the Top Ten Emerging Technologies in Chemistry in 2023 by International Union of Pure and Applied Chemistry (IUPAC)[1]. It has emerged as a sustainable platform for organic synthesis and has exhibited a wide range of applications from academia to industry [2]. However, electrosynthesis has been predominantly performed by using direct current (DC).

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