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Alphaherpesvirus-vectored vaccines against animal diseases: Current progress

Journal of Integrative Agriculture 2020年第8期19卷 1928-1940页

作者： HU Yang WANG Ming-shu chenG An-chun jia Ren-yong YANG Qiao WU Ying liU Ma-feng ZHAO Xin-xin ZHU De-kang chen Shun ZHANG Sha-qiu WANG Yin GAO qun OU xu-min MAO Sai WEN Xing-jian xu Zhi-wen chen Zheng-li ZHU ling LUO Qi-hui TIAN Bin PAN Lei-chang Mujeeb Ur REHMAN liU yun-ya yu Yan-ling ZHANG ling chen Xiao-yue Institute of Preventive Veterinary Medicine Sichuan Agricultural UniversityChengdu 611130P.R.China Key Laboratory of Animal Disease and Human Health of Sichuan Province Sichuan Agricultural UniversityChengdu 611130P.R.China Avian Disease Research Center College of Veterinary MedicineSichuan Agricultural UniversityChengdu 611130P.R.China

Recombinant virus-vectored vaccines are novel agents that can effectively activate specific and nonspecific immunity,are multivalent and multieffective,and have high safety *** alphaherpesviruses have a large genome,contain multiple nonessential regions that do not affect viral replication and are capable of accepting the insertion of an exogenous gene and expressing the antigen ***,animal alphaherpesviruses have a wide host spectrum,can replicate in the host and continuously stimulate the animal to produce immunity to the corresponding pathogen,thus making them ideal carriers for recombinant virus-vectored *** the development of gene-editing technology,recombinant viruses capable of expressing foreign genes can be constructed by various ***,studies on recombinant virusvectored vaccines constructed based on animal alphaherpesviruses have involved poultry,pigs,cattle,sheep,and companion *** have shown that the construction of recombinant animal alphaherpesviruses enables the acquisition of immunity to multiple *** article mainly summarizes the current progress on animal alphaherpesvirus-vectored vaccines,aiming to provide reference for the development of new animal alphaherpesvirus-vectored vaccines.

关键词： recombinant vectored-vaccine recombinant alphaherpesviruses immunity gene-editing exogenous gene

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To Commemorate the 100^(th) Year Anniversary of Forensic Science Education in China: A Brief Review of the lives and Achievements of Forensic Science Pioneers from Zhejiang Province

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Journal of Forensic Science and Medicine 2015年第2期1卷 163-165页

作者： lin-Sheng yu Yan-Yan Fan Jun-Ge Han Xing-Biao li Guang-hua Ye Xiang-Ping Feng Yi liao Cai-qun xu Qi-yu Bao Feng li Department of Forensic Medicine Wenzhou Medical University Institute of Forensic Science Wenzhou Medical University WenzhouZhejiang ProvinceChina Forensic Medical Management Services NashvilleTennesseeUSA

Looking back at modem China's 100-year history of forensic science education,we have found a number of forensic science pioneers from the Zhejiang Province who have made historic contributions to forensic science *** paper briefly reviews the lives and achievements of these pioneers in chronological *** include Song-Ming xu,Ji-Zu Wang,Lv-Gao chen,Jin-Chang Zhu,Ying-Han xu,Mei-yun Wu,jia-Zhen Zhu,Guang-Zhao Huang,Zhan-Pei Zheng,and Shi-Xian *** is only worth writing about if it teaches us about the *** historical review sets examples for both current and future forensic pathologists to learn from the contributions of these pioneers.

关键词： Contributions education forensic history Zhejiang

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Diagnosis and treatment of melamine-associated urinary calculus complicated with acute renal failure in infants and young children

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Chinese Medical Journal 2009年第3期122卷 245-251页

作者： SUN Ning SHEN Ying SUN Qiang li xu-ran jia li-qun ZHANG Gui-ju ZHANG Wei-ping chen Zhi FAN jian-feng jiaNG Ye-ping FENG Dong-chuan ZHANG Rui-feng ZHU Xiao-yu XIAO Hong-zhan Department of Urology Beijing Children's Hospital Affiliated to Capital Medical University Beijing 100045 China Department of Nephrology Beijing Children's Hospital Affiliated to Capital Medical University Beijing 100045 China Diagnostic Imaging Center Beijing Children's Hospital Affiliated to Capital Medical University Beijing 100045 China Department of Surgery xuzhou Children'sHospital Xuzhou Jiangsu 221006 China Department of Pediatric Internal Medicine xuzhou Children'sHospital Xuzhou Jiangsu 221006 China Beijing Institute of Microchemistry Beijing 100091 China

Background Infants in some areas of China developed urinary lithiasis after being fed with powdered milk that was tainted with melamine in 2008 and very small proportion of the infants developed acute renal failure caused by urinary tract calculus obstruction. The aim of this article was to summarize clinical characteristics, diagnosis and treatment of infants with urinary calculus and acute renal failure developed after being fed with melamine tainted formula milk. Methods Data of infant patients with urinary calculus and acute renal failure due to melamine tainted formula milk admitted to the Beijing Children＇s Hospital Affiliated to the Capital Medical University and the xuzhou Children＇s Hospital in 2008 were used to analyze the epidemiological characteristics, clinical manifestations, imaging features as well as effects of 4 types of therapies. Results All the 34 infants with urinary calculus were complicated with acute renal failure, their blood urea nitrogen （BUN） was （24.1±8.2） mmol/L and creatinine （Cr） was （384.2±201.2） umol/L. The chemical analysis on the urinary calculus sampled from 15 of the infants showed that the calculus contained melamine and acidum uricum. The time needed for the four types of therapies for returning Cr to normal was （3.5±1.9） days for cystoscopy group, （2.7±1.1） days for lithotomy group, （3.8±2.3） days for dialysis group, and （2.7±1.6） days for medical treatment group, which had no statistically significant difference （P=-0.508）. Renal failure of all the 34 infants was relieved within 1 to 7 days, averaging （3.00±1.78） days. Conclusions Melamine tainted formula milk may cause urinary calculus and obstructive acute renal failure. It is suggested that firstly the patients with urinary calculus complicated with acute renal failure should be treated with dialysis or medication to correct electrolyte disturbance, in particular hyperkalemia, and then relieve the obstruction with available medical and surgical methods as soon as possibl

关键词： calculus, urinary renal failure, acute, melamine, infant child

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Data processing and data products from 2017 to 2019 campaign of astronomical site testing at Ali,Daocheng and Muztagh-ata

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Research in Astronomy and Astrophysics 2020年第6期20卷 109-130页

作者： Zi-Huang Cao Jin-Xin Hao Lu Feng Hugh RAJones jian li Jing xu li-Yong liu Teng-Fei Song jian-Feng Wang Hua-lin chen Yan-Jie xue Huang Shen Yong-Heng Zhao Zhao-Xiang Qi jian-Rong Shi yuan Tian Zheng Wang Xia Wang Jin-liang Hou Jin-Ming Bai Ji Yang xu Zhou Yong-Qiang Yao jia Yin Ali Esamdin Guang-Xin Pu Peng Wei Chun-Hai Bai Guo-Jie Feng Lu Ma xuan Zhang yu liu Chong Pei Zhi-Xia Shen yun-Ying jiang jian-Feng Tian Xian-qun Zeng Zhi-Gang Hou xu Yang Jun-Bo Zhang Shi-Long liao Dong-Wei Fan yun-Fei xu jian-Ping Xiong Tai-Sheng Yan Xi Zhang Chang-Hua li Yi-Han Tao Si-cheng yu Zhi-Song Cao Hong-Shuai Wang National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Shanghai Astronomical Observatory Chinese Academy of SciencesShanghai 200030China Centre for Astrophysics University of HertfordshireHatfieldUK School of Journalism Communication University of ChinaBeijing 100024China Nanjing Institute of Astronomical Optics&Technology Chinese Academy of SciencesNanjing 210042China Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China yunnan Observatories Chinese Academy of SciencesKunming 650216China Purple Mountain Observatory Chinese Academy of SciencesNanjing 210033China Beijing Kaiyang Space Technology Co. LtdBeijing 100085China

Based on previous site testing and satellite cloud data,Ali,Daocheng and Muztagh-ata have been selected as candidate sites for the Large Optical/Infrared Telescope(LOT) in *** present the data collection,processing,management and quality analysis for our site testing based on using similar *** analyze meteorological data,seeing,background light,cloud and precipitable water vapor data from 2017 March 10 to 2019 March *** also investigated the relative usefulness of our all-sky camera data in comparison to that from the meteorological TERRA satellite data based on a night-by-night comparison of the correlation and consistency between *** find a 6% discrepancy arising from a wide range of factors.

关键词： LOT Site-selection-data processing

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2022年CHINET三级医院细菌耐药监测

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中国感染与化疗杂志 2024年第3期24卷 277-286页

作者：郭燕胡付品朱德妹汪复蒋晓飞徐英春张小江张峰波季萍谢轶肖玉玲王传清付盼徐元宏黄颖孙自镛陈中举孙景勇陈庆褚云卓田素飞胡志东李金俞云松林洁单斌许云敏郭素芳王艳艳魏莲花李可可张泓潘芬胡云建艾效曼卓超苏丹虹郭大文赵金英喻华黄湘宁刘文恩李艳明金炎邵春红徐雪松李威王山梅楚亚菲张利侠马娟周树平周艳朱镭孟晋华董方吕志勇胡芳芳沈瀚周万青贾伟李刚吴劲松卢月梅李继红孙倩段金菊康建邦马晓波郑燕青郭如意朱焱陈运生孟青王世富胡雪飞黄文辉李娟石泉贵杨娟热孜瓦古丽·阿不力米提黄莉莉邵雪君任晓艳李冬张群陈雪黎日海徐洁丽高凯杰徐璐林琳张卓刘健龙付敏郭映辉张文超王增国贾凯夏云孙珊杨慧敏苗艳周明明张诗海刘红娟陈楠李婵沈继录门万琪王鹏张晓威刘彦彦安勇复旦大学附属华山医院抗生素研究所国家卫健委抗生素临床药理重点实验室上海200040 北京协和医院新疆医科大学第一附属医院四川大学华西医院复旦大学附属儿科医院安徽医科大学第一附属医院华中科技大学同济医学院附属同济医院上海交通大学医学院附属瑞金医院中国医科大学附属第一医院天津医科大学总医院浙江大学医学院附属邵逸夫医院昆明医科大学第一附属医院内蒙古医科大学附属医院甘肃省人民医院上海交通大学医学院附属儿童医院北京医院广州医科大学附属第一医院哈尔滨医科大学附属第一医院四川省人民医院中南大学湘雅医院山东省立医院吉林大学中日联谊医院河南省人民医院陕西省人民医院江西省儿童医院山西省儿童医院首都医科大学附属北京儿童医院贵州省人民医院南京大学医学院附属鼓楼医院宁夏医科大学总医院深圳市人民医院河北医科大学第二医院山西医科大学第二医院厦门大学附属第一医院福建省泉州市第一医院深圳市儿童医院山东大学齐鲁儿童医院南昌大学第一附属医院青海大学附属医院西藏军区总医院乌鲁木齐市第一人民医院(乌鲁木齐儿童医院) 苏州大学附属儿童医院云南省腾冲市人民医院重庆医科大学附属儿童医院广西桂平市人民医院河南省儿童医院沈阳市儿童医院湖南省儿童医院河北省儿童医院西安市儿童医院重庆医科大学附属第一医院陕西省神木市医院浙江省儿童医院安徽省儿童医院天津市儿童医院安徽医科大学第四附属医院长春市儿童医院邢台市第九医院复旦大学附属华山医院检验科国家卫健委抗生素临床药理重点实验室上海200040

目的监测2022年CHINET三级医院临床分离菌对抗菌药物的敏感性。方法国内58所三级医院临床分离菌采用纸片扩散法或商品化药敏试验自动测试仪按CHINET统一监测技术方案进行抗菌药物敏感性试验。结果按CLSI M1002022年版折点标准判断。结... 详细信息

目的监测2022年CHINET三级医院临床分离菌对抗菌药物的敏感性。方法国内58所三级医院临床分离菌采用纸片扩散法或商品化药敏试验自动测试仪按CHINET统一监测技术方案进行抗菌药物敏感性试验。结果按CLSI M1002022年版折点标准判断。结果收集2022年1月1日-12月31日上述医院临床分离菌共318013株,其中革兰阳性菌占29.5%,革兰阴性菌占70.5%。金黄色葡萄球菌、表皮葡萄球菌和其他凝固酶阴性葡萄球菌(除假中间葡萄球菌和施氏葡萄球菌外)中甲氧西林耐药株的检出率分别为28.3%、76.7%和77.9%。MRSA中有94.0%的菌株对甲氧苄啶-磺胺甲噁唑敏感;MRSE中有90.8%的菌株对利福平敏感,未发现万古霉素耐药株。肠球菌属中粪肠球菌对多数测试抗菌药物的耐药率均显著低于屎肠球菌,两者中均有少数万古霉素耐药株。2022年儿童和成人中分离的非脑膜炎肺炎链球菌中PSSP的检出率分别为94.2%和95.7%。除克雷伯菌属细菌对碳青霉烯类的耐药率范围为21.7%~23.1%外,其他细菌的耐药率多在13.1%以下。除天然耐药菌株外,其他肠杆菌目细菌对替加环素、黏菌素和多黏菌素B高度敏感,耐药率范围为0.1%~13.3%。铜绿假单胞菌对美罗培南的耐药率从2019年的23.5%下降至2022年的18.0%;鲍曼不动杆菌对美罗培南的耐药率亦从2019年的79.0%下降至2022年的72.5%。结论三级医院临床分离菌对常见抗菌药物的耐药率仍呈增长趋势,但碳青霉烯类耐药肺炎克雷伯菌、铜绿假单胞菌、鲍曼不动杆菌等多种重要耐药细菌的检出率近年来呈现连续下降趋势,提示加强细菌耐药监测,开展多学科联动的方式对控制耐药细菌的流行播散是行之有效的。

关键词：三级医院细菌耐药监测药物敏感性试验碳青霉烯类耐药革兰阴性杆菌

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甲苯浮选2-巯基-β-萘并噻唑分光光度法测定钯

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冶金分析 2006年第3期26卷 77-78页

作者：闫永胜徐婉珍贾立群王斌贝陈婷玉江苏大学化学化工学院江南大学物理系

提出了N2-甲苯浮选,Pd(-2巯-基-β-萘并噻唑(MNT)光度法测定钯的新方法。在硫酸介质中,Pd(与MNT形成组成比为1∶2的红色络合物,该络合物可被N2-甲苯定量浮选,其最大吸收波长位于440 nm处。钯(质量浓度在0.1~100μg/mL范围内符合比尔定律... 详细信息

提出了N2-甲苯浮选,Pd(-2巯-基-β-萘并噻唑(MNT)光度法测定钯的新方法。在硫酸介质中,Pd(与MNT形成组成比为1∶2的红色络合物,该络合物可被N2-甲苯定量浮选,其最大吸收波长位于440 nm处。钯(质量浓度在0.1~100μg/mL范围内符合比尔定律,表观摩尔吸光系数ε440=1.4×***-1,检出限为1.69×10-4mg/L。方法已用于钛合金和天然水中钯的测定。

关键词：浮选 2-巯基-β-萘并噻唑分光光度法钯

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The first data release(DR1) of the LAMOST regular survey

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Research in Astronomy and Astrophysics 2015年第8期15卷 1095-1124页

作者： A-li Luo Yong-Heng Zhao Gang Zhao li-Cai Deng Xiao-Wei liu Yi-Peng Jing Gang Wang Hao-Tong Zhang jian-Rong Shi Xiang-qun Cui Yao-Quan Chu Guo-Ping li Zhong-Rui Bai yue Wu Yan Cai Shu-yun Cao Zi-Huang Cao Jeffrey L.Carlin Hai-yuan chen jian-Jun chen Kun-Xin chen li chen xue-Lei chen Xiao-Yan chen Ying chen Norbert Christlieb jia-Ru Chu chen-Zhou Cui Yi-Qiao Dong Bing Du Dong-Wei Fan Lei Feng jian-Ning Fu Peng Gao xue-Fei Gong Bo-Zhong Gu Yan-Xin Guo Zhan-Wen Han Bo-liang He Jin-liang Hou Yong-Hui Hou Wen Hou Hong-Zhuan Hu Ning-Sheng Hu Zhong-Wen Hu Zhi-Ying Huo Lei jia Fang-Hua jiang Xiang jiang Zhi-Bo jiang Ge Jin Xiao Kong xu Kong Ya-Juan Lei Ai-Hua li Chang-Hua li Guang-Wei li Hai-Ning li jian li Qi li Shuang li Sha-Sha li Xin-Nan li Yan li Yin-Bi li Ye-Ping li yuan liang Chien-cheng lin Chao liu Gen-Rong liu Guan-qun liu Zhi-Gang liu Wen-Zhi Lu yu Luo Yin-Dun Mao Heidi Newberg Ji-Jun Ni Zhao-Xiang Qi Yong-Jun Qi Shi-Yin Shen Huo-Ming Shi Jing Song Yi-Han Song Ding-Qiang Su Hong-Jun Su Zheng-Hong Tang Qing-Sheng Tao yuan Tian Dan Wang Da-Qi Wang Feng-Fei Wang Guo-Min Wang Hai Wang Hong-Chi Wang jian Wang jia-Ning Wang jian-ling Wang jian-Ping Wang Jun-Xian Wang Lei Wang Meng-Xin Wang Shou-Guan Wang Shu-Qing Wang Xia Wang Ya-Nan Wang You Wang yue-Fei Wang You-Fen Wang Peng Wei Ming-Zhi Wei Hong Wu Ke-Fei Wu xue-Bing Wu yu-Zhong Wu Xiao-Zheng Xing ling-Zhe xu Xin-Qi xu Yan xu Tai-Sheng Yan De-Hua Yang Hai-Feng Yang Hui-Qin Yang Ming Yang Zheng-Qiu Yao Yong yu Hui yuan Hai-Bo yuan Hai-Long yuan Wei-Min yuan Chao Zhai En-Peng Zhang Hua-Wei Zhang jian-Nan Zhang li-Pin Zhang Wei Zhang Yong Zhang Yan-Xia Zhang Zheng-Chao Zhang Ming Zhao Fang Zhou xu Zhou Jie Zhu Yong-Tian Zhu Si-cheng Zou Fang Zuo National Astronomical Observatories Chinese Academy of Sciences Department of Astronomy Peking University Shanghai Astronomical Observatory Chinese Academy of Sciences Nanjing Institute of Astronomical Optics & Technology National Astronomical ObservatoriesChinese Academy of Sciences University of Science and Technology of China Department of Physics Applied Physics and AstronomyRensselaer Polytechnic InstituteTroyNY 12180USA Department of Physics and Astronomy Earlham CollegeRichmondIN 47374USA Universitt Heidelberg Zentrum für AstronomieLandessternwarteKnigstuhl 1269117 HeidelbergGermany Department of Astronomy Beijing Normal University yunnan Observatories Chinese Academy of Sciences Purple Mountain Observatory Chinese Academy of Sciences

The Large sky Area Multi-Object Fiber Spectroscopic Telescope（LAMOST） general survey is a spectroscopic survey that will eventually cover approximately half of the celestial sphere and collect 10 million spectra of stars, galaxies and QSOs. Objects in both the pilot survey and the first year regular survey are included in the LAMOST DR1. The pilot survey started in October 2011 and ended in June 2012, and the data have been released to the public as the LAMOST Pilot Data Release in August 2012. The regular survey started in September 2012, and completed its first year of operation in June 2013. The LAMOST DR1 includes a total of 1202 plates containing 2 955 336 spectra, of which 1 790 879 spectra have observed signalto-noise ratio（SNR） ≥ 10. All data with SNR ≥ 2 are formally released as LAMOST DR1 under the LAMOST data policy. This data release contains a total of 2 204 696 spectra, of which 1 944 329 are stellar spectra, 12 082 are galaxy spectra and 5017 are quasars. The DR1 not only includes spectra, but also three stellar catalogs with measured parameters： late A,FGK-type stars with high quality spectra（1 061 918 entries）, A-type stars（100 073 entries）, and M-type stars（121 522 entries）. This paper introduces the survey design, the observational and instrumental limitations, data reduction and analysis, and some caveats. A description of the FITS structure of spectral files and parameter catalogs is also provided.

关键词： techniques: spectroscopic survey—data release—catalog

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基于不变网络模型和故障注入的分布式信息系统故障溯源方法

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控制与决策 2020年第11期35卷 2723-2732页

作者：贾庆轩艾冠群高欣李新鹏阎博陈春旭李军良徐建航刘震宇庞博北京邮电大学自动化学院北京100876 国家电网有限公司北京100031 国网冀北电力有限公司北京100054 南瑞集团(国网电力科学研究院)有限公司北京100192 承德供电公司河北承德067000

针对传统分布式信息系统故障溯源算法对于先验知识依赖严重的问题,提出一种基于不变网络与故障注入相结合的故障溯源方法.首先,利用系统日志中收集到的系统组件运行数据,构建系统的不变网络模型,在此基础上进行节点或组件故障注入及扩... 详细信息

针对传统分布式信息系统故障溯源算法对于先验知识依赖严重的问题,提出一种基于不变网络与故障注入相结合的故障溯源方法.首先,利用系统日志中收集到的系统组件运行数据,构建系统的不变网络模型,在此基础上进行节点或组件故障注入及扩散建模,建立故障网络集;然后,根据原始时间序列取值情况,制定数据质量评价规则以甄别数据是否发生突变;最后,利用实际故障网络与故障网络集中故障网络局部拟合的方式进行故障溯源,并利用数据质量评价规则对该结果进行修正,实现对系统故障源的精确定位.在仿真数据集、某开源系统数据集和某电网调度系统实采数据上的实验结果表明,所提出方法具有更高的准确率.

关键词：信息系统故障溯源时间序列不变网络故障注入局部拟合

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缺血性脑卒中大鼠肠黏膜的损害及小肠运动功能障碍

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首都医科大学学报 2010年第3期31卷 310-314页

作者：啜佳然朱玉群徐立新袁芳徐有青王晨首都医科大学附属北京天坛医院消化内科北京市神经外科研究所病理生理室

目的观察缺血性脑卒中大鼠肠黏膜形态学改变及小肠动力变化,探讨二者之间的关系。方法 48只雄性健康Wistar大鼠分为2组,假手术对照组和缺血性脑卒中模型组,模型组又根据实验终止时间分为5个亚组,每组动物8只。采用改良的Longa线栓法建... 详细信息

目的观察缺血性脑卒中大鼠肠黏膜形态学改变及小肠动力变化,探讨二者之间的关系。方法 48只雄性健康Wistar大鼠分为2组,假手术对照组和缺血性脑卒中模型组,模型组又根据实验终止时间分为5个亚组,每组动物8只。采用改良的Longa线栓法建立大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型。分别于缺血后3h、6h、12h、24h、48h进行光镜及电镜下小肠黏膜病理变化的研究以及利用亚甲蓝染色法检测小肠推进比。结果 MCAO组的小肠推进比与对照组相比显著减低,差异有统计学意义(P<0.05),3h组与6h组比较差异有统计学意义(P<0.01),6h组与12h组、12h组与24h组、24h组与48h组之间比较差异无统计学意义。光镜下MCAO组术后6h即可见肠绒毛肿胀、增粗、缩短,24h见绒毛顶端破损、剥脱,固有层裸露,对照组肠黏膜结构基本完整。电镜下MCAO组小肠绒毛上皮细胞内可见线粒体肿胀、嵴断裂,细胞器空泡变性,细胞间紧密连接增宽,可见凋亡细胞。结论缺血性脑卒中后可出现肠黏膜损伤和小肠动力障碍,二者的发生发展可能互为因果。

关键词：大脑中动脉闭塞模型肠动力肠黏膜

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喜树碱通过抑制HSV-1复制相关基因的表达发挥抗HSV-1作用

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中草药 2018年第17期49卷 4078-4084页

作者：周洁钟鹏禹许群芬陈恬李祖锐崔家旗张倩成都医学院基础医学院四川成都610500 四川大学华西第二医院循证药学中心四川成都610041

目的探讨喜树碱体外抗单纯疱疹病毒Ⅰ型(HSV-1)的作用及其机制。方法不同浓度的喜树碱作用于HSV-1感染的Vero细胞后,采用细胞病变(cytopathologic effects,CPE)法评价喜树碱体外抗HSV-1的作用,计算喜树碱半数毒性浓度(TC_(50))、病毒半... 详细信息

目的探讨喜树碱体外抗单纯疱疹病毒Ⅰ型(HSV-1)的作用及其机制。方法不同浓度的喜树碱作用于HSV-1感染的Vero细胞后,采用细胞病变(cytopathologic effects,CPE)法评价喜树碱体外抗HSV-1的作用,计算喜树碱半数毒性浓度(TC_(50))、病毒半数抑制浓度(IC50)及治疗指数(TI),并通过实时荧光定量PCR(RT-qPCR)法检测喜树碱对HSV-1相关基因m RNA表达的影响。结果喜树碱的TC_(50)为2 153.44 nmol/L,IC_(50)为43.01 nmol/L,TI为50.07。RT-qPCR结果显示,喜树碱能明显抑制HSV-1 UL12、UL42、UL54、TK基因的表达,其抑制作用与喜树碱浓度呈剂量依赖关系。结论喜树碱具有明显抗HSV-1的作用,能抑制HSV-1 UL12、UL42、UL54、TK基因的转录。

关键词：喜树碱单纯疱疹病毒Ⅰ型抗病毒活性实时荧光定量PCR UL12 UL42 UL54 TK

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