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Inhibition of SW620 human colon cancer cells by upregulating mi Rna-145

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World Journal of Gastroenterology 2016年第9期22卷 2771-2778页

作者： Chen Li na xu Yu-Qiang Li Yu Wang Zhi-Tu Zhu Biobank The First Affiliated Hospital of Liaoning Medical University Department of Radiology The First Affiliated Hospital of Liaoning Medical University Department of Oncology The First Affiliated Hospital of Liaoning Medical University

AIM: To investigate the targeted inhibition of proliferation and migration of SW620 human colon cancer cells by upregulating mi Rna-145(mi R-145).METHODS: Forty-five samples of colon cancer tissues and 45 normal control samples were obtained from the biological database of the First Affiliated Hospital of Liaoning Medical University. We performed quantitative analysis of mi R-145 and N-ras expression in tissues; reverse transcriptase polymerase chain reaction analysis of mi R-145 expression in SW620 colon cancer cells and normal colonic epithelial cells; construction of mi R-145 lentiviral vector and determination of mi R-145 expression in SW620 cells transduced with mi R-145 vector; analysis of the effect of mi R-145 overexpression on SW620 cell proliferation; analysis of the effect of mi R-145 overexpression on SW620 cell migration using a wound healing assay; and analysis of the effect ofmi R-145 on N-ras expression using Western blotting. RESULTS: mi R-145 expression was significantly downregulated in colon cancer tissues, with its expression in normal colonic tissues being 4-5-fold higher(two sample t test, P nalysis of variance, P < 0.05). CONCLUSION: mi R-145 is important i

关键词： mi Rna-145 Colorectal cancer Targeted therapy N-ras Lentiviral vector

Activation of CFTR-mediated Cl^- Transport by Magnolin

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Chemical Research in Chinese Universities 2008年第2期24卷 187-191页

作者： JIN Ling-ling LIU Xin SUN Yan LIN Sen ZHOU na xu Li-na YU Bo HOU Shu-guang YANG Hong Liaoning Province Key Laboratory of Biotechnology and Drug Discovery Liaoning Normal UniversityDalian 116029 P. R. China China-Japan Union Hospital Jilin University Changchun 130033P R. China Qianwei Affiliate Hospital Jilin University Changchun 130021 P. R. China

Magnolin is a herbal compound from Magnolia biondii Pamp. It possesses numerous biological activities. Cystic fibrosis transmembrane conductance regulator（CFTR） is an epithelial chloride channel that plays a key role in the fluid secretion of various exocrine organs. In the present study, the activation of CFTR-mediated chloride transport by magnolin is indentified and characterized. In CFTR stably transfected FRT cells, magnolin increases CFTR CI- currents in a concentration-dependent manner. The activation of magnolin on CFTR is rapid, reversible, and cAMP-dependent. Magnolin does not elevate cellular cAMP level, indicating that it activates CFTR by direct binding and interaction with CFTR protein. Magnolin selectively activates wildtype CFTR rather than mutant CFTR. Magnolin may present a novel class of therapeutic lead compound tbr the treatment of diseases associated with reduced CFTR function such as keratoconjunctivitis sicca, idiopathic chronic pancreatiti, and chronic constipation.

关键词： Cystic fibrosis CFTR Mutation Magnolin Activator

Activation Effect of Cathartic natural Compound Rhein to CFTR Chloride Channel

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Chemical Research in Chinese Universities 2006年第3期22卷 312-314页

作者： SHI Lin-lin xu Li-na HOU Shu-guang LIN Sen YANG Hong MA Tong-hui Membrane Channel Research Laboratory Northeast Normal University Changchun 130024 P. R. China Faculty o Life Sciences Liaoning Normal University Dalian 116029 P. R. China China-Japan Union Hospital Jilin University Changchun 130033 P. R. China

The cystic fibrosis transmembrane conductance regulator （CFFR） is a cAMP-activated chloride channel expressed in intestinal exoerine glands, which plays a key role in intestinal fluid secretion. A natural anthraquinone ac tivator of CFTR Cl^- channel, rhein, was identified by screening 217 single compounds from Chinese herbs via a cellbased halide-sensitive fluorescent assay. Rhein activates CFTR Cl^- transportation in a dose-dependent manner in the presence of cAMP with a physiological concentration. This study provides a novel molecular pharmacological mechanism for the laxative drugs in Treditional Chinese Medicine such as aloe, cascara and senna.

关键词： CFTR chloride channel Anthraquinone Laxative drug

natural Compound Curcumin Corrects the Gating Defect of G551DMutant Cystic Fibrosis Transmembrane Conductance Regulator

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Chemical Research in Chinese Universities 2008年第1期24卷 92-95页

作者： HAO Li-ming LIU Xin xu Li-na ZHU na LIN Sen HOU Shu-guang ZHOU na SHI De-cheng SHANG De-jing MA Tong-hui YANG Hong Membrane Channel Research Laboratory Northeast Normal University Changchun 130024 P. R. China China-Japan Union Hospital Jilin University Changchun 130033 P. R. China School of Basic Medicine Jilin University Changchun 130021 P. R. China College of Life Sciences Liaoning Normal University Dalian 116029 P. R.China

In the present study, we identified the natural compound curcumin to be an effective G551D-CFTR activator by cell-based fluorescent assay and electrophysiological measurement. We demonstrated that curcumin can restore the impaired chloride conductance of G551D mutant CFTR. The activity is rapid, reversible, and cAMP-dependent. Our study identified a new natural lead compound for the pharmacological therapy of cystic fibrosis caused by G551D mutation of CFTR.

关键词： Cystic fibrosis CFTR Mutation natural compound Activator

Effects of Yimu Shenghuasan Preparation on the Cytochrome P450 in Endometrial Cells and Immune Function of Dairy Cows

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Agricultural Sciences in China 2010年第10期9卷 1497-1503页

作者： DU Jin-liang QIN Jian-hua CHU Jing-sheng xu Li-na MA Yu-zhong College of Animal Science and Technology Agricultural University of Hebei Baoding 071001 IER.China

In order to investigate the mode of action ofYimu Shenghuasan preparation in endometrial cells of dairy cows, the primary cultured endometrial cells in cows were isolated and the inflammatory models were made by lipopolysaccharide （LPS） induction. The inflammatory cells were treated with gradient concentration of herbal medicine preparation, Yimu Shenghuasan for 48 and 72 h. The expression of cytochrome P450 （CYP450） was detected by Western blot. The amounts of IgG and lgA in sera were also detected in the endometritis of dairy cows. The expression level of CYP450 in the endometrial cells of dairy cow was increased gradually, and the amounts of IgG, IgA were increased significantly as compared with those in the control group. The expression level of CYP450 in the inflammatory cells was increased significantly in the treatment of 2 000 μg mL^-1 of Yimu Shenghuasan after 48 h of treatment.

关键词： dairy cow endometrial cell CYP450 Yimu Shenghuasan preparation immune function

Crystal growth,structure and laser performance of a new Yb^(3+):KBaY(MoO_(4))_(3) crystal

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Journal of Rare Earths 2021年第10期39卷 1246-1255,I0003页

作者： Yi Yu Shimin Wu Xiurong Zhu Xianke Zhang Huajun Yu Jujun Yuan na xu Yu Dong Yeqing Wang School of Physics and Electronics Information Cannan Normal UniversityGanzhou 341000China Department of Applied Physics East China Jiaotong UniversityNanchang 330013China

A Yb^(3+):KBaY(MoO_(4))_(3)(Yb^(3+):KBYM)crystal with dimensions of 51 mm×27 mm × 10 mm was successfully grown by the TSSG *** characteristics of the crystal structure and probability of good optical properties were *** absorption and emission spectra of Yb^(3+):KBYM crystal exhibit broadened bands,with the maximum absorption cross-sections of 1.17 × 10^(-20),1.44×10^(-20) and 1.37 × 10^(-20) cm^(2) at976 nm for X-,Y-and Z-polarizations,*** corresponding absorption FWHMs are as wide as 77,46 and 55 *** well-known re-absorption effect of Yb^(3+) in the crystal is *** methods,the Fiichtbauer-Ladenburg method(FL)and reciprocity method(RM)were adopted to compute the emission cross-sections and results show a certain discrepancy but the errors are *** laser potentiality of the Yb^(3+):KBYM crystal was also evaluated by calculations of minimum inversion fractionβmin,saturation pump intensity Isat,the minimum pump intensity Imin and gain cross-sections *** experiment was carried out and Watt-level continuous wave laser has been *** indicate that the Yb^(3+):KBYM crystal with a disordered structure may be a potential laser media that can be used to generate tunable and ultrashort pulse laser emissions with high quality beam.

关键词： Molybdate crystal Disordered structure TSSG method Spectroscopic properties Laser performance Rare earths

Effect of Annealing Duration and Substrates on Structure and Property of Vanadium Dioxide Films

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Journal of Wuhan University of Technology(Materials Science) 2014年第6期29卷 1117-1123页

作者：储新宏 TAO Haizheng WAN Meinan WANG Shuo NING Zhiyong xu na 赵修建 State Key Laboratory of Silicate Materials for Architectures (Wuhan University of Technology)

Using the oxidation method from vanadium metal thin films by magnetron sputtering, under the fixed annealing parameters of temperature（400 ℃） and oxygen pressure（103 Pa）, we fabricated a series of vanadium dioxide thin films through the change of annealing durations or substrates（quartz glass or AZOcovered glass）. Characterization of the thermochromic properties together with the X-ray diffraction（XRD） and field emission scanning electron microscopy（FE-SEM） indicates that appropriate annealing duration is a key factor to obtain pure VO2 films and AZO-covered glass is more suitable to obtain the VO2 films with high visible transmittance, good crystallinity and larger near-infrared switching efficiencies（maximum 34% at 2000 nm） compared with the substrate of quartz glass. However, VO2 films on quartz glass exhibit narrower loop（7 ℃） with smart reversible response to temperature. Depth profile XPS spectra further indicate that for the films fabricated on quartz glass from thicker V metal films, the existence of low valence vanadium oxides is inevitable and leads to a lower transmittance in the region of visible light.

关键词： VO2 magnetron sputtering oxidation quartz glass AZO thermal hysteresis loop

Two monofluoride-bridged Dy~Ⅲdimers with different magnetization dynamics

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Chinese Chemical Letters 2023年第6期34卷 454-458页

作者： Mengmeng Wang Xixi Meng Ning Liu Yi-Quan Zhang na xu Wei Shi Peng Cheng Department of Chemistry Key Laboratory of Advanced Energy Materials Chemistry(MOE)and Renewable Energy Conversion and Storage Center(RECAST)College of ChemistryNankai UniversityTianjin 300071China Jiangsu Key Lab for NSLSCS School of Physical Science and TechnologyNanjing Normal UniversityNanjing 210023China

As a monoatomic bridge,fluoride ion can transmit efficient magnetic interaction between lanthanide ions but its effect on tuning the magnetization dynamics has not been well ***,two monofluoride-bridged dinuclear dysprosium complexes[Dy_(2)F(bbpen)_(2)(Et OH)_(2)]Br·Et OH(1)and[Dy_(2)F(bbppy)_(2)]Br·2Et OH(2)with Dy-F-Dy angles of~178°and their diamagnetic-ion diluted analogues 1'and 2'were *** studies reveal that 1 and 1'barely show any magnetization dynamics,but 2 and 2'exhibit strong magnetization *** experimental analysis combined with ab initio calculations reveals that the different magnetization dynamics between 1 and 2 mainly originate from the effect of magnetic anisotropy by terminal ligand and bridging group of the chelating ligand,and the fluoride bridge can effectively suppress the quantum tunneling of the magnetization and turn on Orbach process in 2.

关键词： Fluoride ion bridge Dinuclear Dy~Ⅲcomplexes Magnetization dynamics Single-molecule magnet Suppressing QTM

Removal of copper from aqueous solutions by bentonites and the factors affecting it

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Mining Science and Technology 2009年第4期19卷 489-492页

作者： DING Shu-li SUN Yu-zhuang YANG Cui-na xu Bo-hui Key Laboratory for Resources Reconnaissance of Hebei Province Hebei University of Engineering Handan Hebei 056038 China

In order to study the ability of bentonites to remove heavy metal ions from waste water and its factors affecting it,batch sorption experiments of Cu2+ were conducted on Ca-bentonite and na-bentonite under various *** results show that the adsorption process of bentonite accorded with the Freundlich isotherm model and that the sorption results of na-bentonite are better than those of *** behavior of both bentonites was strongly depending on pH,initial concentration and additional amounts of *** are three kinds of adsorption mechanism at different ranges of pH values:the competition adsorption between Cu2+ and H+(pH8.3).The removal rate of bentonite decreases with an increase in the initial metal ion *** maximum adsorption capacity of na-bentonite was 26 mg/g and that of Ca-bentonite 12 mg/*** removal rate of Cu2+ was practically 100% at the initial concentration of 40 mg/L,when 4 g/L of na-bentonite and 14 g/L of Ca-bentonite were added to the solution.

关键词： copper bentonite adsorption cation exchange waste water sorption isotherm