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Differentially expressed miRNAs in anthers may contribute to the fertility of a novel Brassica napus genic male sterile line CN12A

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Journal of Integrative Agriculture 2020年第7期19卷 1731-1742页

作者： DONG yun WANG Yi JIN Feng-wei XING Lijuan FANG Yan ZHANG Zheng-ying ZOU Jun-jie WANG Lei xu miao-yun Crop Research Institute Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China National Key Facility for Crop Gene Resources and Genetic ImprovementBiotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Gansu Provincial Key Lab of Aridland Crop Sciences/Gansu Agricultural University Lanzhou 730070.P.R.China

In Brassica napus L.(rapeseed),complete genic male sterility(GMS)plays an important role in the utilization of *** microRNAs(miRNAs)play essential regulatory roles during bud development,knowledge of how GMS is regulated by miRNAs in rapeseed is rather *** this study,we obtained a novel recessive GMS system,*** sterile line CN12A has defects in tapetal diferentiation and *** sequencing was employed to examine the expression of miRNAs in the buds of CN12A and the fertile line *** identifed 85 known miRNAs and 120 novel miRNAs that were expressed during rapeseed anther *** comparing the expression levels of miRNAs between CN12A and CN12B,19 and 18 known miRNAs were found to be difrentially expressed in 0.5--1.0 mm buds and in 2.5--3.0 mm buds,*** these,the expression levels of 14 miRNAs were higher and the levels of 23 miRNAs were lower in CN12A compared with *** predicted target genes of these iferentially expressed miRNAS encode protein kinases,F-box domain-containing proteins,MADS-box family proteins,SBP-box gene family members,HD ZIP proteins,floral homeotic protein APETAL A 2(AP2)。and nuclear factor Y,subunit *** targets have previously been reported to be involved in pollen development and male sterility,suggesting that miRNAs might act as regulators of GMS in rapeseed ***,RT-qPCR data suggest that one of the dffrentially expressed miRNAs,bna-miR159,plays a role in tapetal differentiation by regulating the expression of transcription factor BnMYB101 and participates in tapetal degradation and infuences callose degradation by manipulating the expression of *** findings contibute to our understanding of the roles of miRNAs during anther development and the occurrence of GMS in rapeseed.

关键词： Brassica napus L. genic male sterility miRNAs targets molecular regulation network

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Evidence of arrested silk growth in maize at high planting density using phenotypic and transcriptional analyses

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Journal of Integrative Agriculture 2022年第11期21卷 3148-3157页

作者： ZHANG Min XING Li-juan REN Xiao-tian ZOU Jun-jie SONG Fu-peng WANG Lei xu miao-yun Biotechnology Research Institute CAAS/Key Laboratory of Agricultural Genomics(Beijing)Ministry of AgricultureBeijing 100081P.R.China College of Bioscience and Resources of Environment Beijing University of AgricultureBeijing 102206P.R.China National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resource College of Resources and EnvironmentShandong Agricultural UniversityTai′an 271018P.R.China

Increasing the planting density is an effective way to increase the yield of maize(Zea mays L.),although it can also aggravate ovary apical abortion-induced bald tips of the ears,which might,in turn,reduce the *** the mechanism underlying the regulation of drought-related abortion in maize is well established,high planting density-related abortion in maize remains poorly ***,the present study was designed to investigate the mechanism underlying the ovary apical abortion response to high *** was achieved by evaluating the effects of four different plant densities(60000 plants ha^(–1)(60 k),90 k,120 k,and 150 k)on plant traits related to plant architecture,the plant ear,flowering time,and silk development in two inbred lines(Zheng58 and PH4CV)and two hybrid lines(Zhengdan958 and Xianyu335).The phenotypes of both inbred and hybrid plants were observed under different planting density treatments,and the high planting density was found to increase the phenotypic performance values of the evaluated *** anthesis–silking interval(ASI)was extended,and the amount of the silk extruded from husks was reduced upon increasing the planting *** silk emergence resulted in asynchronous flowering and ear bald *** of the silk cells revealed that the silk cells became smaller as planting density *** changes in transcript abundances in the silks involved the genes associated with expansive growth rather than carbon *** findings further our understanding of silk growth regulation under high planting density and provide a theoretical basis for further research on improving high planting density breeding in maize.

关键词： maize(Zea mays L.) high planting density bald tip ASI silk expansive growth

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Mitophagy in acute central nervous system injuries:regulatory mechanisms and therapeutic potentials

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Neural Regeneration Research 2025年第9期20卷 2437-2453页

作者： Siyi xu Junqiu Jia Rui Mao Xiang Cao yun xu Department of Neurology Nanjing Drum Tower HospitalClinical College of Jiangsu UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalChinese Academy of Medical Science&Peking Union Medical CollegeNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina State Key Laboratory of Pharmaceutical Biotechnology and Institute of Translational Medicine for Brain Critical Diseases Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina Nanjing Neurology Medical Center NanjingJiangsu ProvinceChina

Acute central nervous system injuries,including ischemic stro ke,intracerebral hemorrhage,subarachnoid hemorrhage,traumatic brain injury,and spinal co rd injury,are a major global health *** optimal therapies and improving the long-term neurological functions of patients with acute central nervous system injuries are urgent *** are susceptible to damage after acute central nervous system injury,and this leads to the release of toxic levels of reactive oxygen species,which induce cell ***,a selective form of autophagy,is crucial in eliminating redundant or damaged mitochondria during these *** evidence has highlighted the significant role of mitophagy in acute central nervous system *** this review,we provide a comprehensive overview of the process,classification,and related mechanisms of *** also highlight the recent developments in research into the role of mitophagy in various acute central nervous system injuries and drug therapies that regulate *** the final section of this review,we emphasize the potential for treating these disorders by focusing on mitophagy and suggest future research paths in this area.

关键词： autophagy intracerebral hemorrhage ischemic stroke mitochondria mitochondrial biogenesis mitochondrial quality control mitophagy spinal cord injury subarachnoid hemorrhage traumatic brain injury

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Inspires effective alternatives to backpropagation:predictive coding helps understand and build learning

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Neural Regeneration Research 2025年第11期20卷 3215-3216页

作者： Zhenghua xu miao Yu Yuhang Song School of Health Sciences and Biomedical Engineering Hebei University of TechnologyTianjinChina Department of Computer Science University of OxfordOxfordUK

Artificial neural networks are capable of machine learning by simulating the hiera rchical structure of the human *** enable learning by brain and machine,it is essential to accurately identify and correct the prediction errors,referred to as credit assignment(Lillicrap et al.,2020).It is critical to develop artificial intelligence by understanding how the brain deals with credit assignment in neuroscience.

关键词： assignment learning enable

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Interfacial Zn2+-solvation regulator towards reversible and stable Zn anode

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Journal of Energy Chemistry 2025年第1期100卷 684-692页

作者： miao Zhou Xiongbin Luo Hang Li Shan Guo Zhuang Tong Xiaotao Zhou xu Li Zhaohui Hou Shuquan Liang Guozhao Fang College of Mechanical Engineering Key Laboratory of Hunan Province for Advanced Carbon-based Functional Materials Hunan Institute of Science and Technology School of Materials Science and Engineering Key Laboratory of Electronic Packaging and Advanced Functional Materials of Hunan Province Central South University College of Materials and Metallurgy Guizhou University National Energy Metal Resources and New Materials Key Laboratory Central South University

Aqueous zinc-ion batteries （AZIBs） are fundamentally challenged by the instability of the electrode/electrolyte interface,predominantly due to irreversible zinc （Zn） deposition and hydrogen ***,the intricate mechanisms behind the electrochemical discrepancies induced by interfacial Zn2+-solvation and deposition behavior demand comprehensive *** molecules endowed with special functional groups （such as hydroxyl,carboxyl,etc.） have the potential to significantly optimize the solvation structure of Zn2+and regulate the interfacial electric double layer （EDL）.By increasing nucleation overpotential and decreasing interfacial free energy,these functional groups facilitate a lower critical nucleation radius,thereby forming an asymptotic nucleation model to promote uniform Zn ***,this study presents a pioneering approach by introducing trace amounts of n-butanol as solvation regulators to engineer the homogenized Zn （H-Zn） anode with a uniform and dense *** interfacial reaction and structure evolution are explored by in/ex-situ experimental techniques,indicating that the H-Zn anode exhibits dendrite-free growth,no by-products,and weak hydrogen evolution,in sharp contrast to the bare ***,the H-Zn anode achieves a remarkable Zn utilization rate of approximately 20% and simultaneously sustains a prolonged cycle life exceeding 500 ***,the H-Zn//NH4V4O10（NVO） full battery showcases exceptional cycle stability,retaining 95.04%capacity retention after 400 cycles at a large current density of 5 A *** study enlightens solvation-regulated additives to develop Zn anode with superior utilization efficiency and extended operational lifespan.

关键词： Solvation

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大豆2-甲基-6-植基-1,4-苯醌甲基转移酶基因(Gm VTE3)的克隆及对转基因烟草种子中生育酚组成的影响

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中国农业科学 2010年第10期43卷 1994-1999页

作者：徐妙云周建张兰范云六王磊中国农业科学院生物技术研究所

【目的】克隆大豆2-甲基-6-植基-1,4-苯醌甲基转移酶基因(GmVTE3),研究其在烟草中过量表达对转基因烟草种子中生育酚(维生素E)组分的影响。【方法】利用EST拼接和RT-PCR技术,从大豆中分离了大豆2-甲基-6-植基-1,4-苯醌甲基转移酶(VTE3)... 详细信息

【目的】克隆大豆2-甲基-6-植基-1,4-苯醌甲基转移酶基因(GmVTE3),研究其在烟草中过量表达对转基因烟草种子中生育酚(维生素E)组分的影响。【方法】利用EST拼接和RT-PCR技术,从大豆中分离了大豆2-甲基-6-植基-1,4-苯醌甲基转移酶(VTE3)的全长cDNA序列,通过烟草转化研究过量表达VTE3对转基因植株维生素E组分的影响。【结果】GmVTE3全长1026bp,编码342个氨基酸,与其它物种的VTE3氨基酸同源性很高,在烟草中过量表达该基因使烟草种子中γ-生育酚与δ-生育酚的比值最高增加2倍。【结论】首次克隆了大豆2-甲基-6-植基-1,4-苯醌甲基转移酶基因GmVTE3,它能够催化烟草植株中2-甲基-6-植基-1,4-苯醌(MPBQ)的甲基化,从而改变烟草种子中生育酚的组成成分。说明可以利用克隆的GmVTE3来改变植物种子中生育酚的组成和提高α-生育酚的含量。

关键词：大豆 2-甲基-6-植基-1,4-苯醌甲基转移酶(VTE3) 生育酚转基因烟草

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Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases

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Neural Regeneration Research 2025年第1期20卷 6-20页

作者： Xiu-yun Zhao De-En xu Ming-Lei Wu Ji-Chuan Liu Zi-Ling Shi Quan-Hong Ma Department of Neurology and Clinical Research Center of Neurological Disease the Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Neuroscience&Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases Soochow UniversitySuzhouJiangsu ProvinceChina Department of Neurology Jiangnan University Medical CenterWuxiJiangsu ProvinceChina

The endoplasmic reticulum,a key cellular organelle,regulates a wide variety of cellular *** reticulum autophagy,one of the quality control systems of the endoplasmic reticulum,plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover,remodeling,and *** this review,we briefly describe the endoplasmic reticulum quality control system,and subsequently focus on the role of endoplasmic reticulum autophagy,emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular *** also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative *** summary,this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve *** review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

关键词： autophagy endoplasmic reticulum endoplasmic reticulum autophagy endoplasmic reticulum quality control system endoplasmic reticulum receptors endoplasmic reticulum-associated degradation neurodegeneration neurodegenerative disease selective autophagy unfolded protein response

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铁路货车链式手制动机拉杆存在问题的分析及改进建议

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铁道车辆 2010年第5期48卷 42-43页

作者：郁陈陈早明许妙愠上海铁路局杭州北车辆段浙江杭州311100

1 故障情况链式手制动机拉杆3根圆钢组焊处裂纹现象较突出,严重影响了车辆运行安全.运用部门在日常检查中经常发现手制动机拉杆裂纹、折断故障及手制动机拉杆托架座脱焊故障.

关键词：手制动机拉杆铁路货车链式折断故障裂纹现象上海铁路局运行安全

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大豆24kDa油体蛋白基因在大肠杆菌中的表达

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中国农业科学 2005年第2期38卷 234-240页

作者：徐妙云刘德虎李刚强中国农业科学院生物技术研究所中国农业科学院生物技术研究所北京100081

分别构建了含全长大豆24　kDa油体蛋白基因、与大肠杆菌热敏毒素B亚基和定居因子CS6　B亚基基因以串联方式融合的全长大豆24　kDa油体蛋白基因、缺失N端74个氨基酸编码序列的油体蛋白基因及缺失C端152个氨基酸编码序列油体蛋白基因等4... 详细信息

分别构建了含全长大豆24　kDa油体蛋白基因、与大肠杆菌热敏毒素B亚基和定居因子CS6　B亚基基因以串联方式融合的全长大豆24　kDa油体蛋白基因、缺失N端74个氨基酸编码序列的油体蛋白基因及缺失C端152个氨基酸编码序列油体蛋白基因等4个大肠杆菌表达载体。除N端缺失的油体蛋白基因外,其它3个载体的IPTG诱导表达产物对***生长表现出明显的抑制作用,菌液浓度与对照菌株相比,至少降低了3倍;而N端缺失了222个编码核苷酸的油体蛋白基因则对宿主细菌细胞的生长繁殖不再表现有任何毒性。进一步通过SDS-PAGE和Western印迹反应检测各表达载体的IPTG诱导表达产物时发现,仅有N端缺失的24　kDa油体蛋白基因片段在宿主细胞BL21(DE3)和Rosseta(DE3)中有重组油体蛋白积累。

关键词：大豆油体蛋白表达大肠杆菌

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microRNA与植物花发育调控的研究进展

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中国农业科技导报 2011年第2期13卷 9-16页

作者：徐妙云王磊中国农业科学院生物技术研究所国家农作物基因资源与基因改良重大科学工程北京100081

microRNAs(miRNAs)是约21nt的非编码RNA,主要在转录后水平调节基因的活性。miRNAs通过与靶基因的互补位点结合从而降解靶基因mRNA或抑制其翻译。miRNAs参与调控植物生长发育的多个方面,包括生长、开花、代谢、激素应答、生物与非生物胁... 详细信息

microRNAs(miRNAs)是约21nt的非编码RNA,主要在转录后水平调节基因的活性。miRNAs通过与靶基因的互补位点结合从而降解靶基因mRNA或抑制其翻译。miRNAs参与调控植物生长发育的多个方面,包括生长、开花、代谢、激素应答、生物与非生物胁迫。综述了miRNAs在植物花发育中的研究进展,以期为更好地了解miRNA在此过程中的作用机制,并应用于改良植物的农艺性状及培育优良品种奠定基础。

关键词： miRNA 植物调控发育花器官发育

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