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Robotic-assisted surgery for pediatric choledochal cyst: Case report and literature review

World Journal of Clinical Cases 2018年第7期6卷 143-149页

作者： Xian-Qiang Wang Shu-juan xu Zheng Wang Yuan-Hong Xiao Jing xu Zhen-Dong Wang Di-Xiang Chen Department of Pediatrics PLA General Hospital Center of Anesthesia and Operation PLA General Hospital

Our paper describes the key surgical points of pediatric choledochocystectomy performed completely by Da Vinci robotic system. A choledochocystectomy was safely carried out for a girl at our hospital, and without any complication. Then systematic literature review was done to discuss the methods of intestine surgery and intestinal anastomosis, the use of 3 rd robotic arm, the surgical safety and advantages comparing open and laparoscopic surgery. We systematically reviewed choledochocystectomy for children performed by robotic surgery. We included a total of eight domestic and foreign reports and included a total of 86 patients, whose average age was 6.3(0.3-15.9) years; the maleto-female ratio was 1:3.5(19:67). Seven patients experienced conversion to open surgery, and the surgery success rate was 91.9%(79/86). The average total operation time was 426(180-520) min, the operation time on the machine was 302(120-418) min, 11 cases used the number 3 arm, and the remaining mainly used the hitch-stitch technique to suspend the stomach wall and liver. Forty-seven patients underwent pull-through intestine and intestinal anastomosis, and 39 patients underwent complete robotic intestine surgery and intestinal anastomosis. The hospitalization time of roboticassisted choledochocystectomy was 8.8 d. Eight patientshad biliary fistula and were all cured by conservative treatment and continuous observation. One patient had anastomotic stenosis, and one patient had wound dehiscence, both cured by surgery. Choledochocystectomy for children performed by completely robotic surgery and Roux-en-Y hepaticojejunostomy is safe and feasible. The initial experience shows that this surgical approach has a clearer field than the traditional endoscopy, and its operation is more flexible, the surgery is more accurate, and the injury is smaller. With the advancement of technology and the accumulation of surgeons' experience, robotic surgery may become a new trend in this surgical procedure.

关键词： Choledochocystectomy Robotic surgery Pediatrics Congenital choledochal cysts

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The abscisic acid-responsive transcriptional regulatory module CsERF110-CsERF53 orchestrates citrus fruit coloration

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Plant Communications 2024年第11期5卷 157-172页

作者： Quan Sun Zhengchen He Di Feng Ranran Wei Yingzi Zhang Junli Ye Lijun Chai juan xu Yunjiang Cheng Qiang xu Xiuxin Deng National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops Huazhong Agricultural UniversityWuhan 430070China National Research Center for Apple Engineering and Technology Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTaianShandong 271018China Hubei Hongshan Laboratory Wuhan Hubei 430070China These authors contributed equally to this article

Carotenoid biosynthesis is closely associated with abscisic acid(ABA)during the ripening process of non-climacteric fruits,but the regulatory mechanism that links ABA signaling to carotenoid metabolism remains largely ***,we identified two master regulators of ABA-mediated citrus fruit coloration,CsERF110 and CsERF53,which activate the expression of carotenoid metabolism genes(CsGGPPS,CsPSY,CsPDS,CsCRTISO,CsLCYB2,CsLCYE,CsHYD,CsZEP,and CsNCED2)to facilitate carotenoid *** investigations showed that CsERF110 not only activates the expression of CsERF53 by binding to its promoter but also interacts with CsERF53 to form the transcriptional regulatory module *** also discovered a positive feedback regulatory loop between the ABA signal and carotenoid metabolism regulated by the transcriptional regulatory module *** results reveal that the CsERF110-CsERF53 module responds to ABA signaling,thereby orchestrating citrus fruit *** the importance of carotenoid content for citrus and many other carotenoid-rich crops,the revelation of molecular mechanisms that underlie ABA-mediated carotenoid biosynthesis in plants will facilitate the development of transgenic/gene-editing approaches,further contributing to improving the quality of citrus and other carotenoid-rich crops.

关键词： citrus abscisic acid ABA fruit coloration carotenoid metabolism ethylene responsive factor ERF transcriptional regulatory module

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近50年云南省怒江、澜沧江流域气象干旱研究

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北京大学学报（自然科学版） 2017年第5期53卷 964-972页

作者：徐娟保山学院资源环境学院保山678000

基于怒江、澜沧江云南段31个气象站点1965—2013年的月降水、气温资料,计算两个流域各生物气候区的标准化降水蒸散指数(SPEI)。分析近50年的年、季节、月尺度的干旱过程、干旱发生频率及强度,揭示怒江、澜沧江云南段干旱发生的时空和强... 详细信息

基于怒江、澜沧江云南段31个气象站点1965—2013年的月降水、气温资料,计算两个流域各生物气候区的标准化降水蒸散指数(SPEI)。分析近50年的年、季节、月尺度的干旱过程、干旱发生频率及强度,揭示怒江、澜沧江云南段干旱发生的时空和强度演变特征。结果表明,自20世纪70年代后期以来,研究区季节、月尺度干旱整体上呈显著增加趋势,尤其是冬季;两个流域内各气候区干旱特征的空间分异不明显,干旱发生与全省性的大范围干旱发生较为一致;近50年来,研究区气温的显著升高可能比降水的微弱减少对其干旱变化贡献更大。

关键词：标准化降水蒸散指数(SPEI) 气象干旱怒江澜沧江

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Construction of Nanocarriers Based on Endogenous Cell Membrane and Their Application in Nanomedicine

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Chinese Journal of Chemistry 2022年第13期40卷 1623-1640页

作者： Yingshu Guo Wenxin Li Shiwei Liu Dan Jing Yifan Wang Qingfang Feng Kaixiang Zhang Jing-juan xu Shandong Provincial Key Laboratory of Molecular Engineering School of Chemistry and Chemical EngineeringQilu University of Technology(Shandong Academyof Sciences)JinanShandong 250353China School of Chemistry and Chemical Engineering Linyi UniversityLinyiShandong 276005China State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Centre of Chemistry for Life Sciences Nanjing University163Xianlin RoadNanjingJiangsu 210023China School of Pharmaceutical Sciences Zhengzhou UniversityZhengzhouHenan 450001China

Inspired by the adaptation of cells to the surrounding environment,the combination of cell membrane and nanomaterials has gradually become a new type of biomimetic *** construction of endogenous membrane biomimetic nanocarriers(EMBNs)not only retains the structure of membrane surface protein,but also has the properties of nanoparticles(NPs),also provides the ability of natural interaction between nanomaterials and organisms,thus overcoming the severe challenges faced by traditional nanodelivery systems in clinical *** this paper,the construction methods of EMBNs are reviewed,focusing on a simple method to prepare membrane ***,EMBNs were classified according a variety of cell ***,the unique advantages of EMBNs in nanomedicine are introduced,including improving biocompatibility,homologous targeting ability,prolonging blood circulation time and immune escape *** nanomedicine,such as targeted delivery,phototherapy,immunotherapy and tumor imaging,multifaceted biological interfaces through membrane masking provide a new approach for the development of multifunctional NPs.

关键词： Biomimeticnanocomposite Membranes Nanomedicine Antitumoragents Drugdelivery

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Warming increases soil carbon input in a Sibiraea angustata-dominated alpine shrub ecosystem

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Journal of Plant Ecology 2022年第2期15卷 335-346页

作者： Mei Liu Jia-Hao Wen Ya-Mei Chen Wen-juan xu Qiong Wang Zhi-Liang Ma Ecological Security and Protection Key Laboratory of Sichuan Province Mianyang Normal UniversityMianyang 621000China Department of Watershed Sciences and the Ecology Center Utah State UniversityLoganUT 84321USA College of Life Sciences China West Normal UniversityNanchong 637009China

Plant-derived carbon(C)inputs via foliar litter,root litter and root exudates are key drivers of soil organic C ***,the responses of these three input pathways to climate warming have rarely been studied in alpine *** employing a 3-year warming experiment(increased by 1.3℃),we investigated the effects of warming on the relative C contributions from foliar litter,root litter and root exudates from Sibiraea angustata,a dominant shrub species in an alpine shrubland on the eastern Qinghai-Tibetan *** soil organic C inputs from foliar litter,root litter and root exudates were 77.45,90.58 and 26.94 g C m^(-2),*** only slightly increased the soil organic C inputs from foliar litter and root litter by 8.04 and 11.13 g C m^(-2),but significantly increased the root exudate C input by 15.40 g C m^(-2).Warming significantly increased the relative C contributions of root exudates to total C inputs by 4.6%but slightly decreased those of foliar litter and root litter by 2.5%and 2.1%,*** results highlight that climate warming may stimulate plant-derived C inputs into soils mainly through root exudates rather than litter in alpine shrublands on the Qinghai-Tibetan Plateau.

关键词： soil organic carbon foliar litter root litter root exudates climate warming alpine shrub Qinghai-Tibetan Plateau

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Clinical characteristics of acute non-varicose upper gastrointestinal bleeding and the effect of endoscopic hemostasis

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World Journal of Clinical Cases 2024年第9期12卷 1597-1605页

作者： Xiao-juan Wang Yu-Peng Shi Li Wang Ya-Ni Li Li-juan xu Yue Zhang Shuang Han Department of Gastroenterology Honghui Hospital Affiliated to Medicine College of Xi’an Jiaotong UniversityXi'an 710054Shaanxi ProvinceChina

BACKGROUND Acute non-variceal upper gastrointestinal bleeding(ANVUGIB)constitutes a prevalent emergency within Gastroenterology,encompassing 80%-90%of all gastrointestinal hemorrhage *** condition is distinguished by its abrupt onset,swift progression,and notably elevated mortality *** To gather clinical data from patients with ANVUGIB at our hospital in order to elucidate the clinical characteristics specific to our institution and analyze the therapeutic effectiveness of endoscopic *** We retrospectively retrieved the records of 532 patients diagnosed with ANVUGIB by endoscopy at our hospital between March 2021 and March 2023,utilizing our medical record *** pertaining to general patient information,etiological factors,disease outcomes,and other relevant variables were meticulously collected and *** Among the 532 patients diagnosed with ANVUGIB,the male-to-female ratio was 2.91:1,with a higher prevalence among ***,43.6%of patients presented with black stool as their primary complaint,while 27.4%had hematemesis as their initial *** admission,17%of patients exhibited both hematemesis and black stool,while most ANVUGIB patients primarily complained of overt gastrointestinal *** routine blood examinations at admission revealed that 75.8%of patients had anemia,with 63.4%experiencing moderate to severe anemia,and 1.5%having extremely severe anemia(hemoglobin<30 g/L).With regard to etiology,53.2%of patients experienced bleeding without a definitive trigger,24.2%had a history of using gastric mucosa-irritating medications,24.2%developed bleeding after alcohol consumption,2.8%attributed it to improper diet,1.7%to emotional excitement,and 2.3%to fatigue preceding the bleeding ***-induced ANVUGIB was more prevalent in the elderly than middle-aged and young individuals,while bleeding due to alcohol consumption showed the opposite ***,diet-related bleeding was more common

关键词： Acute non-varicose upper gastrointestinal bleeding Clinical characteristics Cause of disease Endoscopic homeostatic therapy

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Reappraisement and refinement of zircon U-Pb isotope and trace element analyses by LA-ICP-MS

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Chinese Science Bulletin 2010年第15期55卷 1535-1546页

作者： LIU YongSheng HU ZhaoChu ZONG KeQing GAO ChangGui GAO Shan xu juan CHEN HaiHong State Key Laboratory of Geological Processes and Mineral Resources Faculty of Earth Sciences China University of Geosciences Wuhan 430074 China State Key Laboratory of Continental Dynamics Department of Geology Northwest University Xi'an 710069 China

A protocol was established for simultaneous measurements of zircon U-Pb ages and trace elements by LA-ICP-MS at spot sizes of 16 32 μ*** was accomplished by introducing N 2 into ICP to increase the *** obtained U-Pb ages for zircon standards GJ-1,TEMORA and SK10-2 are consistent with the preferred values within about 1% uncertainty (2σ) by simple external calibration against zircon standard *** data reduction softwares could yield different uncertainties for calculation of U-Pb *** commercially available program GLITTER4.4 could apply an improper uncertainty calculation strategy,but it may yield artificial high precisions for single *** trace element analyses indicate that Si is not an ideal internal standard for zircon when calibrated against the NIST *** against the NIST glasses using Si as an internal standard,a systematic deviation of 10% 30% was found for most trace elements including ***,the trace element compositions of zircon can be accurately measured by calibration against multiple reference materials with natural compositions (e.g.,BCR-2G,BHVO-2G and BIR-1G),or calibration against NIST SRM 610 and using Zr as an internal *** of two pieces of GJ-1 demonstrate that it is relatively homogenous for most trace elements (except for Ti).

关键词： Pb同位素微量元素元素分析 ICP MS法锆石 LA 固体火箭发动机

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Transcription factor CsTT8 promotes fruit coloration by positively regulating the methylerythritol 4-phosphate pathway and carotenoid biosynthesis pathway in citrus (Citrus spp.)

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Horticulture Research 2023年第11期10卷 71-81页

作者： Quan Sun Zhengchen He Ranran Wei Yingzi Yin Junli Ye Lijun Chai Zongzhou Xie Wenwu Guo juan xu Yunjiang Cheng Qiang xu Xiuxin Deng National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops Huazhong Agricultural UniversityWuhan 430070China National Research Center for Apple Engineering and Technology Shandong Agricultural UniversityTaianShandong 271018China Hubei Hongshan Laboratory Wuhan Hubei 430070China

Carotenoids directly influence citrus fruit color and nutritional value,which is critical to consumer *** the potential molecular mechanism underlying carotenoid metabolism is of great importance for improving fruit *** the well-established carotenoid biosynthetic pathways,the molecular regulatory mechanism underlying carotenoid metabolism remains poorly *** previous studies have reported that the Myc-type basic helix-loop-helix(bHLH)transcription factor(TF)regulates citrus proanthocyanidin *** analyses further showed that overexpression of CsTT8 could significantly promote carotenoid accumulation in transgenic citrus calli,but its regulatory mechanism is still *** the present study,we found that overexpression of CsTT8 enhances carotenoid content in citrus fruit and calli by increasing the expression of CsDXR,CsHDS,CsHDR,CsPDS,CsLCYE,CsZEP,and CsNCED2,which was accompanied by changes in the contents of abscisic acid and *** in vitro and in vivo assays indicated that CsTT8 directly bound to the promoters of CsDXR,CsHDS,and CsHDR,the keymetabolic enzymes of the methylerythritol 4-phosphate(MEP)pathway,thus providing precursors for carotenoid biosynthesis and transcriptionally activating the expression of these three *** addition,CsTT8 activated the promoters of four key carotenoid biosynthesis pathway genes,CsPDS,CsLCYE,CsZEP,and CsNCED2,directly promoting carotenoid *** study reveals a novel network of carotenoid metabolism regulated by *** findings will contribute to manipulating carotenoid metabolic engineering to improve the quality of citrus fruit and other crops.

关键词： citrus Citrus metabolism

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Climate change comparison between Arctic and other areas in the Northern Hemisphere since the last Interstade

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Journal of Geographical Sciences 2007年第1期17卷 43-50页

作者： xu juan WANG Guo ZHANG Baiping Institute of Geographic Sciences and Natural Resources Research CAS Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Based on temperature reconstruction and proxy data from 14 sites in the Northern Hemisphere, this paper focused on comparing the cycles of temperature variations between the Arctic and other areas, including Atlantic, Europe, China, Asia, Pacific, Indian Ocean, and America during the transition from the last Interstade to the Last Glacial Maximum, from the Last Glacial Maximum to megathermal period in Holocene and the transition of the Little Ice Age （LIA） by the methods of Singular Spectrum Analysis （SSA） and Maximum Entropy Spectrum （MES）. The results showed that environmental changes in the Arctic are most similar to that in the North American and better similar to Asia, Atlantic and Pacific, the least similar to Indian Ocean and Europe. The 1500-year oscillation of temperature existed both in Arctic and Europe.

关键词： climatic cycles comparison Arctic Northern Hemisphere last Interstade

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Meteorological Drought in Nujiang and Lancang River Basins during Recent 50 Years

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Journal of Landscape Research 2018年第2期10卷 58-64,68页

作者： xu juan School of Resources and Environment Baoshan University

Drought is one of the main natural disasters that cause economic loss in the basins of international rivers such as Nujiang and Lancang rivers. Based on the monthly precipitation and temperature data of 31 meteorological stations in Nujiang and Lancang river basins in Yunnan Province during 1965-2013, the standardized precipitation evapotranspiration index(SPEI) in each of the two bio-climate zones was calculated. In addition, the drought process in annual, seasonal and monthly scale was analyzed respectively to reveal the spatial and temporal characteristics and the intensity variation of meteorological drought in Nujiang and Lancang river basins in Yunnan Province. The results showed that there was a significant increasing trend in seasonal(especially winter's) and monthly drought since the late 1970 s; the drought occurred in the two bio-climate zones showed no obvious spatial distinction, and it was synchronized with that occurred throughout Yunnan Province; and in the recent 50 years, the significant increase of drought in the study area may be attributed to the significant rise in temperature, rather than the slight decline of the precipitation.

关键词： Standardized precipitation evapotranspiration index(SPEI) Meteorological drought Nujiang River Lancang River

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